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The map label for this gene is asrB [H]

Identifier: 78224621

GI number: 78224621

Start: 3851101

End: 3851946

Strand: Direct

Name: asrB [H]

Synonym: Gmet_3431

Alternate gene names: 78224621

Gene position: 3851101-3851946 (Clockwise)

Preceding gene: 78224620

Following gene: 78224622

Centisome position: 96.34

GC content: 64.89

Gene sequence:

>846_bases
ATGTGCGATCACAGTAAAAACATCTATCTCCCCCAACTCGCCACGATCAAGGAGATCGTCGACGAGACCGCCGACATCCG
CACCTTCCGGCTTGAGTTCCAGGACGAGACGGTGCGGGAGAACTTCACCTTCCGGGCCGGCCAGTTCGCCGAGTACTCGG
CCTTCGGGGCCGGGGAGGCCACCTTCTGCATCGCCTCGGCGCCGACCCGCAAAGGGTACATCGAGTGCTGCTTCCGGGCC
GTGGGGCGGGTCACCGAGGCGCTCCGGCAACTGGAGGTGGGCGACGCCATCGGCGTGCGGGGCCCCTACGGCAACTCCTT
TCCCATCGAGGAGTTCTTCGGCAGGAATCTCGTCTTTGTGGCCGGGGGGATCGCGCTCCCGCCGCTGCGGACCCTCATCG
GGCAGTGCCTGGACTGGCGGGAGAAGTTCGGCGACATTACGATTGTCTACGGCGCCCGCACCGAGGGGGATCTGGTCTAC
AAGCGGGAGCTCACGGAGTGGGAAGAGCGCAGCGACGTGCGGCTCGTGAAGACCGTGGACCCCGGCGGGAACTCACCCGA
CTGGGACGGCAAGGTCGGCTTTGTCCCCACGATCCTGGAAGAGGCGGCTCCCGCGGCGGACAACACCATCGCCCTTGTGT
GCGGCCCCCCCATCATGATCAAGTTCACCCTGCCGGTGCTGGAGAAGCTCGGTTTCAGTGACAACGCCATCTACACCACC
CTGGAAAACCGGATGAAGTGCGGCCTCGGCAAGTGCGGCCGCTGCAACGTAGGGAACGTCTATGTCTGCAAGGACGGCCC
GGTCTTCACTGCCGCTCAGGTCAAGGCCATGCCCCAGGAGTTCTGA

Upstream 100 bases:

>100_bases
AGGGACCGGCAAAGACTAAGAAAGGAACCAGGGACCGGAAGCCCTGGACTGGTAAAGATTTTTACTGGTCCCCGGTCCCC
GGTCCCCAATCACCGATTTT

Downstream 100 bases:

>100_bases
AACCTCCCATCACCCTCACCTCTCAGCAGCCGGCAGCACTGGTCCTTGCCCGTGGGATTTTCCCCCCGGCCCCCTTCCGT
TGCGCCAGAACAGGCCCTTT

Product: oxidoreductase FAD/NAD(P)-binding protein

Products: NAD(P); FMNH2; 2-octaprenyl-6-hydroxyphenol [C]

Alternate protein names: NA

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MCDHSKNIYLPQLATIKEIVDETADIRTFRLEFQDETVRENFTFRAGQFAEYSAFGAGEATFCIASAPTRKGYIECCFRA
VGRVTEALRQLEVGDAIGVRGPYGNSFPIEEFFGRNLVFVAGGIALPPLRTLIGQCLDWREKFGDITIVYGARTEGDLVY
KRELTEWEERSDVRLVKTVDPGGNSPDWDGKVGFVPTILEEAAPAADNTIALVCGPPIMIKFTLPVLEKLGFSDNAIYTT
LENRMKCGLGKCGRCNVGNVYVCKDGPVFTAAQVKAMPQEF

Sequences:

>Translated_281_residues
MCDHSKNIYLPQLATIKEIVDETADIRTFRLEFQDETVRENFTFRAGQFAEYSAFGAGEATFCIASAPTRKGYIECCFRA
VGRVTEALRQLEVGDAIGVRGPYGNSFPIEEFFGRNLVFVAGGIALPPLRTLIGQCLDWREKFGDITIVYGARTEGDLVY
KRELTEWEERSDVRLVKTVDPGGNSPDWDGKVGFVPTILEEAAPAADNTIALVCGPPIMIKFTLPVLEKLGFSDNAIYTT
LENRMKCGLGKCGRCNVGNVYVCKDGPVFTAAQVKAMPQEF
>Mature_281_residues
MCDHSKNIYLPQLATIKEIVDETADIRTFRLEFQDETVRENFTFRAGQFAEYSAFGAGEATFCIASAPTRKGYIECCFRA
VGRVTEALRQLEVGDAIGVRGPYGNSFPIEEFFGRNLVFVAGGIALPPLRTLIGQCLDWREKFGDITIVYGARTEGDLVY
KRELTEWEERSDVRLVKTVDPGGNSPDWDGKVGFVPTILEEAAPAADNTIALVCGPPIMIKFTLPVLEKLGFSDNAIYTT
LENRMKCGLGKCGRCNVGNVYVCKDGPVFTAAQVKAMPQEF

Specific function: This enzyme catalyzes the hydrogen sulfide production from sulfite. It is strictly anaerobic. It is regulated by electron acceptors rather than by cysteine [H]

COG id: COG0543

COG function: function code HC; 2-polyprenylphenol hydroxylase and related flavodoxin oxidoreductases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Homo sapiens, GI49574502, Length=249, Percent_Identity=24.0963855421687, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17563926, Length=171, Percent_Identity=27.4853801169591, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17563928, Length=173, Percent_Identity=30.0578034682081, Blast_Score=68, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6322146, Length=182, Percent_Identity=27.4725274725275, Blast_Score=73, Evalue=4e-14,
Organism=Drosophila melanogaster, GI281366101, Length=177, Percent_Identity=31.0734463276836, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24662934, Length=177, Percent_Identity=31.0734463276836, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24662938, Length=177, Percent_Identity=31.0734463276836, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI116007806, Length=176, Percent_Identity=27.8409090909091, Blast_Score=71, Evalue=8e-13,
Organism=Drosophila melanogaster, GI116007804, Length=176, Percent_Identity=27.8409090909091, Blast_Score=71, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012165
- InterPro:   IPR019480
- InterPro:   IPR017927
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR017938
- InterPro:   IPR014260 [H]

Pfam domain/function: PF10418 DHODB_Fe-S_bind; PF00970 FAD_binding_6; PF00175 NAD_binding_1 [H]

EC number: 1.5.1.29 [C]

Molecular weight: Translated: 31038; Mature: 31038

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
3.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCDHSKNIYLPQLATIKEIVDETADIRTFRLEFQDETVRENFTFRAGQFAEYSAFGAGEA
CCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEEECCCHHHHCCCCCCCCCCHHCCCCCCCE
TFCIASAPTRKGYIECCFRAVGRVTEALRQLEVGDAIGVRGPYGNSFPIEEFFGRNLVFV
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHCCCEEEE
AGGIALPPLRTLIGQCLDWREKFGDITIVYGARTEGDLVYKRELTEWEERSDVRLVKTVD
ECCCCCCHHHHHHHHHHHHHHHHCCEEEEEECCCCCCEEEEHHHHHHHCCCCEEEEEEEC
PGGNSPDWDGKVGFVPTILEEAAPAADNTIALVCGPPIMIKFTLPVLEKLGFSDNAIYTT
CCCCCCCCCCCCCCHHHHHHHCCCCCCCEEEEEECCCEEEEEEHHHHHHCCCCCCEEEEE
LENRMKCGLGKCGRCNVGNVYVCKDGPVFTAAQVKAMPQEF
HHHHHHCCCCCCCCCCCCCEEEECCCCEEEHHHHHCCCCCC
>Mature Secondary Structure
MCDHSKNIYLPQLATIKEIVDETADIRTFRLEFQDETVRENFTFRAGQFAEYSAFGAGEA
CCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEEECCCHHHHCCCCCCCCCCHHCCCCCCCE
TFCIASAPTRKGYIECCFRAVGRVTEALRQLEVGDAIGVRGPYGNSFPIEEFFGRNLVFV
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHCCCEEEE
AGGIALPPLRTLIGQCLDWREKFGDITIVYGARTEGDLVYKRELTEWEERSDVRLVKTVD
ECCCCCCHHHHHHHHHHHHHHHHCCEEEEEECCCCCCEEEEHHHHHHHCCCCEEEEEEEC
PGGNSPDWDGKVGFVPTILEEAAPAADNTIALVCGPPIMIKFTLPVLEKLGFSDNAIYTT
CCCCCCCCCCCCCCHHHHHHHCCCCCCCEEEEEECCCEEEEEEHHHHHHCCCCCCEEEEE
LENRMKCGLGKCGRCNVGNVYVCKDGPVFTAAQVKAMPQEF
HHHHHHCCCCCCCCCCCCCEEEECCCCEEEHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD(P)H2; FMN [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD(P)H2; FMN; 2 2-octaprenylphenol; O2 [C]

Specific reaction: NAD(P)H2 + FMN = NAD(P) + FMNH2 2 2-octaprenylphenol + O2 = 2 2-octaprenyl-6-hydroxyphenol [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1704886; 11677609 [H]