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The map label for this gene is metK

Identifier: 78222504

GI number: 78222504

Start: 1449945

End: 1451114

Strand: Direct

Name: metK

Synonym: Gmet_1290

Alternate gene names: 78222504

Gene position: 1449945-1451114 (Clockwise)

Preceding gene: 78222503

Following gene: 78222507

Centisome position: 36.27

GC content: 61.28

Gene sequence:

>1170_bases
ATGGAGATGAAGGACTATATTTTTACCTCCGAATCGGTTTCCGAGGGACATCCCGACAAGGTCGCGGATCAGGTGTCGGA
TTCCATTCTTGATGCGATTCTTGCACAGGATCCCAGGGCCCGCGTTGCCTGCGAAACCCTGGTAACTACCGGTATGGCGG
TTATTGCCGGCGAGATCACCACGAGTGCCGTGGTGGACTATCCGAAGGTTGTCCGCGAGACCATCCGGGAGATCGGCTAC
AACGACTCGGCCATGGGGTTCGACTGGGAAACCTGCGCGGTGCTCGTTTCCATTGACAAACAGTCCCCTGACATCTCCCA
GGGGGTCACGGAGGGCGAGGGGATGTTCAAGGAGCAGGGGGCCGGCGACCAGGGGCTCATGTTCGGCTATGCCTGCACCG
AAACCCCCGAACTCATGCCGATGTCCATCACCTATGCCCACAAGCTGACCCAGAAGCTGGCCGAGGTGCGCAAGAACGGT
GTGCTGGACTTCCTTCGTCCCGACTCCAAGTCCCAAGTCTCCGTCGAATATGTGGACGACAAGCCGGTGCGCGTCGATAC
GGTCGTCATCTCATCCCAGCATACGCCGGAGGTTTCCTACGAAGCCATCAAGGAAGGGATCATCGAGGAGGTCGTGAAGA
AGATCATCCCGGCCCACCTCATGGACGAACGGACCCGCATGCTCATCAACCCCACCGGCCGCTTTGTCGTCGGCGGTCCC
ATGGGTGACTGCGGCCTGACCGGGCGCAAGATCATCGTCGACAGCTACGGCGGCCATGGTGCCCATGGCGGCGGCGCCTT
CTCCGGCAAGGACCCCTCGAAAGTGGACCGCTCTGCCGCCTACATGGGGCGTTACGTTGCCAAGAACCTGGTAGCCGCCG
GCCTGTGCGAGAAGTGCGAGGTTCAGGTAGCCTACGCCATCGGCGTTGCCGAGCCGGTTTCGGTCATGGTCGATACCGCC
GGCACCGGCAAGATCTCCTCCAAGCGGATTGCCGAGATCGTCCGCGAGGTCTTCGACCTGCGGCCGCGCGCCATCATCGA
GCAGCTGGACCTTCTCCGCCCCATTTATCGCAAGACCGCCGCTTACGGTCACTTTGGCCGTGAGCTGCCCGAGTTCACCT
GGGAGCGGACCGACAAGGTGTATATGATCCGCCAGAAGGCGGGAATCTAG

Upstream 100 bases:

>100_bases
CGTTTTCTTCCTTGACATCCGTTGCTGCCAGCTTATACAATTCACACCTTTGCAAATCATTGAGCAGGCCATTTTCACCA
GCGAGGAGGAACGATCAGCG

Downstream 100 bases:

>100_bases
CGAGATAGTAAGATAGTAAGTAGCGAGATAGTAAAAAGGCCGGATGACTTTGCGTCACCCGGCCTTTTCTCCTTTTGTAT
CCTACTCCTTCACGATCTCA

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase

Number of amino acids: Translated: 389; Mature: 389

Protein sequence:

>389_residues
MEMKDYIFTSESVSEGHPDKVADQVSDSILDAILAQDPRARVACETLVTTGMAVIAGEITTSAVVDYPKVVRETIREIGY
NDSAMGFDWETCAVLVSIDKQSPDISQGVTEGEGMFKEQGAGDQGLMFGYACTETPELMPMSITYAHKLTQKLAEVRKNG
VLDFLRPDSKSQVSVEYVDDKPVRVDTVVISSQHTPEVSYEAIKEGIIEEVVKKIIPAHLMDERTRMLINPTGRFVVGGP
MGDCGLTGRKIIVDSYGGHGAHGGGAFSGKDPSKVDRSAAYMGRYVAKNLVAAGLCEKCEVQVAYAIGVAEPVSVMVDTA
GTGKISSKRIAEIVREVFDLRPRAIIEQLDLLRPIYRKTAAYGHFGRELPEFTWERTDKVYMIRQKAGI

Sequences:

>Translated_389_residues
MEMKDYIFTSESVSEGHPDKVADQVSDSILDAILAQDPRARVACETLVTTGMAVIAGEITTSAVVDYPKVVRETIREIGY
NDSAMGFDWETCAVLVSIDKQSPDISQGVTEGEGMFKEQGAGDQGLMFGYACTETPELMPMSITYAHKLTQKLAEVRKNG
VLDFLRPDSKSQVSVEYVDDKPVRVDTVVISSQHTPEVSYEAIKEGIIEEVVKKIIPAHLMDERTRMLINPTGRFVVGGP
MGDCGLTGRKIIVDSYGGHGAHGGGAFSGKDPSKVDRSAAYMGRYVAKNLVAAGLCEKCEVQVAYAIGVAEPVSVMVDTA
GTGKISSKRIAEIVREVFDLRPRAIIEQLDLLRPIYRKTAAYGHFGRELPEFTWERTDKVYMIRQKAGI
>Mature_389_residues
MEMKDYIFTSESVSEGHPDKVADQVSDSILDAILAQDPRARVACETLVTTGMAVIAGEITTSAVVDYPKVVRETIREIGY
NDSAMGFDWETCAVLVSIDKQSPDISQGVTEGEGMFKEQGAGDQGLMFGYACTETPELMPMSITYAHKLTQKLAEVRKNG
VLDFLRPDSKSQVSVEYVDDKPVRVDTVVISSQHTPEVSYEAIKEGIIEEVVKKIIPAHLMDERTRMLINPTGRFVVGGP
MGDCGLTGRKIIVDSYGGHGAHGGGAFSGKDPSKVDRSAAYMGRYVAKNLVAAGLCEKCEVQVAYAIGVAEPVSVMVDTA
GTGKISSKRIAEIVREVFDLRPRAIIEQLDLLRPIYRKTAAYGHFGRELPEFTWERTDKVYMIRQKAGI

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family

Homologues:

Organism=Homo sapiens, GI5174529, Length=384, Percent_Identity=57.5520833333333, Blast_Score=435, Evalue=1e-122,
Organism=Homo sapiens, GI4557737, Length=374, Percent_Identity=56.951871657754, Blast_Score=427, Evalue=1e-120,
Organism=Escherichia coli, GI1789311, Length=387, Percent_Identity=64.0826873385013, Blast_Score=526, Evalue=1e-151,
Organism=Caenorhabditis elegans, GI17538498, Length=380, Percent_Identity=57.6315789473684, Blast_Score=437, Evalue=1e-123,
Organism=Caenorhabditis elegans, GI17538494, Length=380, Percent_Identity=57.1052631578947, Blast_Score=435, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI25145633, Length=384, Percent_Identity=57.2916666666667, Blast_Score=433, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI17551082, Length=378, Percent_Identity=56.6137566137566, Blast_Score=422, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI32565905, Length=380, Percent_Identity=51.0526315789474, Blast_Score=367, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI32565911, Length=329, Percent_Identity=56.2310030395137, Blast_Score=363, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI25145635, Length=333, Percent_Identity=55.8558558558559, Blast_Score=359, Evalue=2e-99,
Organism=Caenorhabditis elegans, GI32565903, Length=302, Percent_Identity=55.2980132450331, Blast_Score=324, Evalue=4e-89,
Organism=Caenorhabditis elegans, GI32565909, Length=86, Percent_Identity=61.6279069767442, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI32565907, Length=44, Percent_Identity=68.1818181818182, Blast_Score=72, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6320710, Length=381, Percent_Identity=57.4803149606299, Blast_Score=444, Evalue=1e-126,
Organism=Saccharomyces cerevisiae, GI6323209, Length=375, Percent_Identity=58.1333333333333, Blast_Score=444, Evalue=1e-125,
Organism=Drosophila melanogaster, GI45552159, Length=374, Percent_Identity=57.7540106951872, Blast_Score=416, Evalue=1e-116,
Organism=Drosophila melanogaster, GI24580529, Length=374, Percent_Identity=57.7540106951872, Blast_Score=416, Evalue=1e-116,
Organism=Drosophila melanogaster, GI24580537, Length=374, Percent_Identity=57.7540106951872, Blast_Score=416, Evalue=1e-116,
Organism=Drosophila melanogaster, GI24580533, Length=374, Percent_Identity=57.7540106951872, Blast_Score=416, Evalue=1e-116,
Organism=Drosophila melanogaster, GI24580535, Length=374, Percent_Identity=57.7540106951872, Blast_Score=416, Evalue=1e-116,
Organism=Drosophila melanogaster, GI24580531, Length=374, Percent_Identity=57.7540106951872, Blast_Score=416, Evalue=1e-116,
Organism=Drosophila melanogaster, GI24580527, Length=374, Percent_Identity=57.4866310160428, Blast_Score=414, Evalue=1e-116,
Organism=Drosophila melanogaster, GI24580525, Length=374, Percent_Identity=57.4866310160428, Blast_Score=414, Evalue=1e-116,
Organism=Drosophila melanogaster, GI24580541, Length=361, Percent_Identity=56.786703601108, Blast_Score=391, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580539, Length=122, Percent_Identity=59.8360655737705, Blast_Score=152, Evalue=4e-37,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METK_GEOMG (Q39W48)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_384251.1
- ProteinModelPortal:   Q39W48
- SMR:   Q39W48
- STRING:   Q39W48
- GeneID:   3739151
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_1290
- eggNOG:   COG0192
- HOGENOM:   HBG443662
- OMA:   IYYAHRL
- PhylomeDB:   Q39W48
- ProtClustDB:   PRK05250
- BioCyc:   GMET269799:GMET_1290-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00086
- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636
- PANTHER:   PTHR11964
- PIRSF:   PIRSF000497
- TIGRFAMs:   TIGR01034

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N; SSF55973 S-AdoMet_synt

EC number: =2.5.1.6

Molecular weight: Translated: 42541; Mature: 42541

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS00376 ADOMET_SYNTHASE_1; PS00377 ADOMET_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEMKDYIFTSESVSEGHPDKVADQVSDSILDAILAQDPRARVACETLVTTGMAVIAGEIT
CCCCHHEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCHHHHHHHHH
TSAVVDYPKVVRETIREIGYNDSAMGFDWETCAVLVSIDKQSPDISQGVTEGEGMFKEQG
HHHHHCCHHHHHHHHHHCCCCCCCCCCCHHHEEEEEEECCCCCCHHHCCCCCCCCHHHCC
AGDQGLMFGYACTETPELMPMSITYAHKLTQKLAEVRKNGVLDFLRPDSKSQVSVEYVDD
CCCCCEEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHHCCCEEECCCCCCCEEEEEEECC
KPVRVDTVVISSQHTPEVSYEAIKEGIIEEVVKKIIPAHLMDERTRMLINPTGRFVVGGP
CCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEECCC
MGDCGLTGRKIIVDSYGGHGAHGGGAFSGKDPSKVDRSAAYMGRYVAKNLVAAGLCEKCE
CCCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHC
VQVAYAIGVAEPVSVMVDTAGTGKISSKRIAEIVREVFDLRPRAIIEQLDLLRPIYRKTA
EEEEEEECCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
AYGHFGRELPEFTWERTDKVYMIRQKAGI
HHHHHHHHHHHHHHCCCCCEEEEECCCCC
>Mature Secondary Structure
MEMKDYIFTSESVSEGHPDKVADQVSDSILDAILAQDPRARVACETLVTTGMAVIAGEIT
CCCCHHEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCHHHHHHHHH
TSAVVDYPKVVRETIREIGYNDSAMGFDWETCAVLVSIDKQSPDISQGVTEGEGMFKEQG
HHHHHCCHHHHHHHHHHCCCCCCCCCCCHHHEEEEEEECCCCCCHHHCCCCCCCCHHHCC
AGDQGLMFGYACTETPELMPMSITYAHKLTQKLAEVRKNGVLDFLRPDSKSQVSVEYVDD
CCCCCEEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHHCCCEEECCCCCCCEEEEEEECC
KPVRVDTVVISSQHTPEVSYEAIKEGIIEEVVKKIIPAHLMDERTRMLINPTGRFVVGGP
CCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEECCC
MGDCGLTGRKIIVDSYGGHGAHGGGAFSGKDPSKVDRSAAYMGRYVAKNLVAAGLCEKCE
CCCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHC
VQVAYAIGVAEPVSVMVDTAGTGKISSKRIAEIVREVFDLRPRAIIEQLDLLRPIYRKTA
EEEEEEECCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
AYGHFGRELPEFTWERTDKVYMIRQKAGI
HHHHHHHHHHHHHHCCCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA