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The map label for this gene is ptsI [H]

Identifier: 78222503

GI number: 78222503

Start: 1448070

End: 1449836

Strand: Direct

Name: ptsI [H]

Synonym: Gmet_1289

Alternate gene names: 78222503

Gene position: 1448070-1449836 (Clockwise)

Preceding gene: 78222502

Following gene: 78222504

Centisome position: 36.23

GC content: 57.44

Gene sequence:

>1767_bases
ATGGATTCGGCGAGGAATGAAACGAGGATTTTTCGCGGAATAGAAGCCTCGGCCGGCATCGCCATCGGCACGGCACGCTT
GACCGACCGCAGTCGCGTGGCGGTCACCGAGGTTTCCATCGGCGATGAAGAGGTGGAACTGGAGTGCGCCCGCTTCCTCG
CAGCGCTTCAGGATGCCCGGGAAGAGCTCCTTGCTGTCAAGGAGCATCTGGCGTCAAGCCGCGGTCCGGAACACCTCTAT
GTTGTCGATAGCCACCTTCTGATCCTTGAAGATGGGATGCTGACCCGTGGCACCCTTGATCTGATTGCCGGCGAGAAGAT
CAATGCCGAAGCTGCCCTCAAGCGTAACCTCCTAAAGTTCAAAGAATTCTTTTCCGCCATTGAAGATGAGTATCTGCGCG
AACGGGCCGGTGATGTAGAAACCGTCGTCGAGCGTATCATGCGCGCCATGGTGGGAAAACGACAGGAGTGCATCGGCTCC
ATTGACGGTGGCAAGATCGTCATCGTCGCCCACGACCTCTCTCCAACCGACATTCTCCAGATCGACAAGAACAACGTTAT
CGGCTTCATAACTGATCTGGGGGGACGAACATCACACACCGCCATACTGGCCAGGGCACTGGAAATTCCTGCAGTCGTAG
GTCTTGAAACGATTACCGGCGAAGACATCGACGGACTGCCGATAATCATCGATGGCACTGACGGGACGGTGGTCGTCAAT
CCCGACCAGGATACCTTTCGCGATTACCTGCGGCGCAAGCAACGTTACGACTATCTGGAGCAGGAATTTCTTAAACTGCG
GGATCTCCCTGCCGAGACCATAGACGGCCATCGGATTTCACTCATGGGTAACATTGAGTTTGCCGAGGAGATCCCAACGC
TCAAGGGTCACGGAGGTGAGGGGATCGGTCTTTACCGGACCGAGATGCTTTACATGAACCGCCAGGGACTTCCCGACGAA
GAAGAGCAATACTTGGCCTACTCCGCAATCGTCGAAGCAATGGCACCTCTGCCGATAACCATTCGCACCCTCGACGTGGG
GGGCGATAAACTCGCCACCGACCTCCATCTGGAAGACGAGATGAACCCGGCCCTGGGACTGCGCGCCATTCGTCTGTCTC
TCCGTAAACCCGAGGTCTTCAAGACCCAGTTGCGGGCAATTCTGCGCGCCAGCGTACGGGGAAAAGTCCGGATATTCTTC
CCGATGATTTCGGGGGTAGAGGAAGTGCGCGCCGCCAAGGCGATTCTGGAGGAAGCGAAAAATGAGCTCCGTGCTGAAGG
AGTTCCCTTCGACAACGCCATTGATGTTGGCATCATGATCGAGATTCCGTCGGCTGTGCTCGTTGCCGACATTCTTGCCC
GGGAGGTGGATTTCTTCAGCGTCGGCACCAATGACCTCATCCAGTACTCGTTGGCCATCGACCGAACCAATGAGCACCTG
GCGCACCTCTATCAACCGATGCACCCCGCTGTTCTCCGATCTCTCCGGACGGTGGTCGATGCGGCCCATGCCGCCGGAAT
CCGCGCCTGCATGTGTGGCGAGGTGGCGGGCGAGTCACGCTATCTTCCCATACTCCTCGGTTTGGGGTTTGATGAACTGT
CCATGACCGGCGTGTCGATTCCGCGAGTCAAGAAAGTCCTGCGCCGGGCCGTCAAGGCTGACGCCCAGTTGCTCGTCTCC
CGGGCCTTCACTTTTTCCACCGCCGCCGAAGTGGAGGCCTTCCTGAACAGCGAGATTTCCGCACGGTTTTCCGAGAGTTT
CGACTGA

Upstream 100 bases:

>100_bases
ATCATGATGCTGGCCGCGGCCAAGGGAACACTTATCAGGGTGAAAATTGAGGGGGAGGACGAGGCGGAAGCAATGGCCGC
CCTCGGAGAGCTTATAACCA

Downstream 100 bases:

>100_bases
TTTCTTCCCGTTTTCTTCCTTGACATCCGTTGCTGCCAGCTTATACAATTCACACCTTTGCAAATCATTGAGCAGGCCAT
TTTCACCAGCGAGGAGGAAC

Product: phosphoenolpyruvate--protein phosphotransferase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 588; Mature: 588

Protein sequence:

>588_residues
MDSARNETRIFRGIEASAGIAIGTARLTDRSRVAVTEVSIGDEEVELECARFLAALQDAREELLAVKEHLASSRGPEHLY
VVDSHLLILEDGMLTRGTLDLIAGEKINAEAALKRNLLKFKEFFSAIEDEYLRERAGDVETVVERIMRAMVGKRQECIGS
IDGGKIVIVAHDLSPTDILQIDKNNVIGFITDLGGRTSHTAILARALEIPAVVGLETITGEDIDGLPIIIDGTDGTVVVN
PDQDTFRDYLRRKQRYDYLEQEFLKLRDLPAETIDGHRISLMGNIEFAEEIPTLKGHGGEGIGLYRTEMLYMNRQGLPDE
EEQYLAYSAIVEAMAPLPITIRTLDVGGDKLATDLHLEDEMNPALGLRAIRLSLRKPEVFKTQLRAILRASVRGKVRIFF
PMISGVEEVRAAKAILEEAKNELRAEGVPFDNAIDVGIMIEIPSAVLVADILAREVDFFSVGTNDLIQYSLAIDRTNEHL
AHLYQPMHPAVLRSLRTVVDAAHAAGIRACMCGEVAGESRYLPILLGLGFDELSMTGVSIPRVKKVLRRAVKADAQLLVS
RAFTFSTAAEVEAFLNSEISARFSESFD

Sequences:

>Translated_588_residues
MDSARNETRIFRGIEASAGIAIGTARLTDRSRVAVTEVSIGDEEVELECARFLAALQDAREELLAVKEHLASSRGPEHLY
VVDSHLLILEDGMLTRGTLDLIAGEKINAEAALKRNLLKFKEFFSAIEDEYLRERAGDVETVVERIMRAMVGKRQECIGS
IDGGKIVIVAHDLSPTDILQIDKNNVIGFITDLGGRTSHTAILARALEIPAVVGLETITGEDIDGLPIIIDGTDGTVVVN
PDQDTFRDYLRRKQRYDYLEQEFLKLRDLPAETIDGHRISLMGNIEFAEEIPTLKGHGGEGIGLYRTEMLYMNRQGLPDE
EEQYLAYSAIVEAMAPLPITIRTLDVGGDKLATDLHLEDEMNPALGLRAIRLSLRKPEVFKTQLRAILRASVRGKVRIFF
PMISGVEEVRAAKAILEEAKNELRAEGVPFDNAIDVGIMIEIPSAVLVADILAREVDFFSVGTNDLIQYSLAIDRTNEHL
AHLYQPMHPAVLRSLRTVVDAAHAAGIRACMCGEVAGESRYLPILLGLGFDELSMTGVSIPRVKKVLRRAVKADAQLLVS
RAFTFSTAAEVEAFLNSEISARFSESFD
>Mature_588_residues
MDSARNETRIFRGIEASAGIAIGTARLTDRSRVAVTEVSIGDEEVELECARFLAALQDAREELLAVKEHLASSRGPEHLY
VVDSHLLILEDGMLTRGTLDLIAGEKINAEAALKRNLLKFKEFFSAIEDEYLRERAGDVETVVERIMRAMVGKRQECIGS
IDGGKIVIVAHDLSPTDILQIDKNNVIGFITDLGGRTSHTAILARALEIPAVVGLETITGEDIDGLPIIIDGTDGTVVVN
PDQDTFRDYLRRKQRYDYLEQEFLKLRDLPAETIDGHRISLMGNIEFAEEIPTLKGHGGEGIGLYRTEMLYMNRQGLPDE
EEQYLAYSAIVEAMAPLPITIRTLDVGGDKLATDLHLEDEMNPALGLRAIRLSLRKPEVFKTQLRAILRASVRGKVRIFF
PMISGVEEVRAAKAILEEAKNELRAEGVPFDNAIDVGIMIEIPSAVLVADILAREVDFFSVGTNDLIQYSLAIDRTNEHL
AHLYQPMHPAVLRSLRTVVDAAHAAGIRACMCGEVAGESRYLPILLGLGFDELSMTGVSIPRVKKVLRRAVKADAQLLVS
RAFTFSTAAEVEAFLNSEISARFSESFD

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=574, Percent_Identity=41.8118466898955, Blast_Score=450, Evalue=1e-127,
Organism=Escherichia coli, GI48994992, Length=463, Percent_Identity=40.3887688984881, Blast_Score=315, Evalue=6e-87,
Organism=Escherichia coli, GI1788726, Length=505, Percent_Identity=36.8316831683168, Blast_Score=307, Evalue=1e-84,
Organism=Escherichia coli, GI1789193, Length=573, Percent_Identity=35.9511343804538, Blast_Score=305, Evalue=4e-84,
Organism=Escherichia coli, GI1787994, Length=496, Percent_Identity=25.8064516129032, Blast_Score=96, Evalue=6e-21,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 64860; Mature: 64860

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSARNETRIFRGIEASAGIAIGTARLTDRSRVAVTEVSIGDEEVELECARFLAALQDAR
CCCCCCHHHHHHCCCCCCCEEEEEEEECCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHH
EELLAVKEHLASSRGPEHLYVVDSHLLILEDGMLTRGTLDLIAGEKINAEAALKRNLLKF
HHHHHHHHHHHHCCCCCEEEEEECEEEEEECCCCCCCCHHHHCCCCCCHHHHHHHHHHHH
KEFFSAIEDEYLRERAGDVETVVERIMRAMVGKRQECIGSIDGGKIVIVAHDLSPTDILQ
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHCCCCCCEEEEEEECCCCCCEEE
IDKNNVIGFITDLGGRTSHTAILARALEIPAVVGLETITGEDIDGLPIIIDGTDGTVVVN
ECCCCEEEEEECCCCCCHHHHHHHHHHHCCHHCCEEEECCCCCCCCEEEEECCCCEEEEC
PDQDTFRDYLRRKQRYDYLEQEFLKLRDLPAETIDGHRISLMGNIEFAEEIPTLKGHGGE
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCHHHHCCCCCCCCCC
GIGLYRTEMLYMNRQGLPDEEEQYLAYSAIVEAMAPLPITIRTLDVGGDKLATDLHLEDE
CEEEEEHEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHEEEEEECCC
MNPALGLRAIRLSLRKPEVFKTQLRAILRASVRGKVRIFFPMISGVEEVRAAKAILEEAK
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEEHHHCCHHHHHHHHHHHHHHH
NELRAEGVPFDNAIDVGIMIEIPSAVLVADILAREVDFFSVGTNDLIQYSLAIDRTNEHL
HHHHCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHCCEEECCCHHHEEEEEEECCCHHHH
AHLYQPMHPAVLRSLRTVVDAAHAAGIRACMCGEVAGESRYLPILLGLGFDELSMTGVSI
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHCCCCCC
PRVKKVLRRAVKADAQLLVSRAFTFSTAAEVEAFLNSEISARFSESFD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCC
>Mature Secondary Structure
MDSARNETRIFRGIEASAGIAIGTARLTDRSRVAVTEVSIGDEEVELECARFLAALQDAR
CCCCCCHHHHHHCCCCCCCEEEEEEEECCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHH
EELLAVKEHLASSRGPEHLYVVDSHLLILEDGMLTRGTLDLIAGEKINAEAALKRNLLKF
HHHHHHHHHHHHCCCCCEEEEEECEEEEEECCCCCCCCHHHHCCCCCCHHHHHHHHHHHH
KEFFSAIEDEYLRERAGDVETVVERIMRAMVGKRQECIGSIDGGKIVIVAHDLSPTDILQ
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHCCCCCCEEEEEEECCCCCCEEE
IDKNNVIGFITDLGGRTSHTAILARALEIPAVVGLETITGEDIDGLPIIIDGTDGTVVVN
ECCCCEEEEEECCCCCCHHHHHHHHHHHCCHHCCEEEECCCCCCCCEEEEECCCCEEEEC
PDQDTFRDYLRRKQRYDYLEQEFLKLRDLPAETIDGHRISLMGNIEFAEEIPTLKGHGGE
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCHHHHCCCCCCCCCC
GIGLYRTEMLYMNRQGLPDEEEQYLAYSAIVEAMAPLPITIRTLDVGGDKLATDLHLEDE
CEEEEEHEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHEEEEEECCC
MNPALGLRAIRLSLRKPEVFKTQLRAILRASVRGKVRIFFPMISGVEEVRAAKAILEEAK
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEEHHHCCHHHHHHHHHHHHHHH
NELRAEGVPFDNAIDVGIMIEIPSAVLVADILAREVDFFSVGTNDLIQYSLAIDRTNEHL
HHHHCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHCCEEECCCHHHEEEEEEECCCHHHH
AHLYQPMHPAVLRSLRTVVDAAHAAGIRACMCGEVAGESRYLPILLGLGFDELSMTGVSI
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHCCCCCC
PRVKKVLRRAVKADAQLLVSRAFTFSTAAEVEAFLNSEISARFSESFD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]