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The map label for this gene is prfB [H]

Identifier: 78066892

GI number: 78066892

Start: 2492728

End: 2493903

Strand: Direct

Name: prfB [H]

Synonym: Bcep18194_A5423

Alternate gene names: 78066892

Gene position: 2492728-2493903 (Clockwise)

Preceding gene: 78066891

Following gene: 78066893

Centisome position: 67.48

GC content: 63.18

Gene sequence:

>1176_bases
TTGCGTCACCGGGCAACCCTGCCGATCGGCTATAATTCCGCTTTTTTACGAAGCAAGAATAGCGAACGATCATGGAAGCG
GAACGTCTCAACGCGATCGAAAGCTCCCTGCTCGACCTGCGCACCCGCGCGGGCGAGCTTCGGGGGTATCTTTGACTACG
ACGCCAAAGCTGCGCGTCTGACCGAAGTCAACAAGGAACTCGAAGACCCGAACGTCTGGAACGATTCGAAGAACGCCCAG
GCGCTCGGTCGCGAGAAGAAGCTGCTCGAGGGCGTCGTCACGACGCTGACGGCGCTCGATAGCGACCTGCGTGACGCGCT
CGACCTGTTCGAGTTGGCCCGCGAGGAAGGCGACGAGGACACCCTCGTCGCGTCGGAAGAAGACGCCGCGAAGCTCGCGT
CGCGCGTCGGCGACATCGAGTTCCGCCGGATGTTCTCGAACCCGGCCGATCCGAACAACTGCTTCATCGACATCCAGGCC
GGCGCCGGCGGCACCGAGGCGTGCGACTGGGCATCGATGCTGTTGCGCCAGTACCTGCGCTACTGCGAACGCAAGGGCTT
CAAGGCCGAAGTGCTCGAAGAGTCCGACGGCGACGTCGCCGGCATCAAGAATGCGACGGTCAAGGTCTCGGGCGAATACG
CGTACGGCTTCCTGCGCACCGAAACAGGCATCCACCGCCTCGTGCGCAAGTCGCCGTTCGACTCGTCGGGCGGCCGCCAC
ACGTCGTTCTCGTCGGTGTTCGTCTATCCGGAAATCGACGACTCGATCGAAGTCGAGATCAACCCGGCCGACCTGCGCAT
CGACACGTACCGCGCATCGGGCGCGGGCGGTCAGCACATCAACAAGACCGACTCCGCGGTGCGGATCACGCACATGCCGA
CCGGTATCGTCGTGCAGTGCCAGAACGACCGCTCGCAGCACCGCAACCGCGCCGAAGCGATGGCGATGCTGAAATCGCGC
CTGTACGAAGTTGAAATGCGCAAGCGCCAGGCCGAACAGGACAAGCTCGAATCGAGCAAGACCGATGTGGGCTGGGGCCA
CCAGATCCGTTCGTACGTGCTCGACCAGAGCCGCGTGAAGGACCTGCGCACGAACGTCGAAATGAGCAACACGCGCGCCG
TCCTCGACGGCGACCTCGACGACTTCATCAGCGCGAGCCTCAAACAGGGCGTGTAA

Upstream 100 bases:

>100_bases
CGAACACGCGGCCGGCTGAGCGCGGGCCGCCCGGCCTGCGCAGCCCGCCGAGGCATGCACGCCGCGCCGGACGGGGCCGC
GGCGCGCGTGCGAGCACCGT

Downstream 100 bases:

>100_bases
GCGCCGCGGCGCGACCCTGCCGGGTCGCGCCCCCTTGCCCTTCCCGTTTGCGTTGCCGCACCCACTCCGAATTCCGACCA
TCATGACCGAACCGACCCAA

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MRHRATLPIGYNSAFLRSKNSERSWKRNVSTRSKAPCSTCAPARASFGGIFDYDAKAARLTEVNKELEDPNVWNDSKNAQ
ALGREKKLLEGVVTTLTALDSDLRDALDLFELAREEGDEDTLVASEEDAAKLASRVGDIEFRRMFSNPADPNNCFIDIQA
GAGGTEACDWASMLLRQYLRYCERKGFKAEVLEESDGDVAGIKNATVKVSGEYAYGFLRTETGIHRLVRKSPFDSSGGRH
TSFSSVFVYPEIDDSIEVEINPADLRIDTYRASGAGGQHINKTDSAVRITHMPTGIVVQCQNDRSQHRNRAEAMAMLKSR
LYEVEMRKRQAEQDKLESSKTDVGWGHQIRSYVLDQSRVKDLRTNVEMSNTRAVLDGDLDDFISASLKQGV

Sequences:

>Translated_391_residues
MRHRATLPIGYNSAFLRSKNSERSWKRNVSTRSKAPCSTCAPARASFGGIFDYDAKAARLTEVNKELEDPNVWNDSKNAQ
ALGREKKLLEGVVTTLTALDSDLRDALDLFELAREEGDEDTLVASEEDAAKLASRVGDIEFRRMFSNPADPNNCFIDIQA
GAGGTEACDWASMLLRQYLRYCERKGFKAEVLEESDGDVAGIKNATVKVSGEYAYGFLRTETGIHRLVRKSPFDSSGGRH
TSFSSVFVYPEIDDSIEVEINPADLRIDTYRASGAGGQHINKTDSAVRITHMPTGIVVQCQNDRSQHRNRAEAMAMLKSR
LYEVEMRKRQAEQDKLESSKTDVGWGHQIRSYVLDQSRVKDLRTNVEMSNTRAVLDGDLDDFISASLKQGV
>Mature_391_residues
MRHRATLPIGYNSAFLRSKNSERSWKRNVSTRSKAPCSTCAPARASFGGIFDYDAKAARLTEVNKELEDPNVWNDSKNAQ
ALGREKKLLEGVVTTLTALDSDLRDALDLFELAREEGDEDTLVASEEDAAKLASRVGDIEFRRMFSNPADPNNCFIDIQA
GAGGTEACDWASMLLRQYLRYCERKGFKAEVLEESDGDVAGIKNATVKVSGEYAYGFLRTETGIHRLVRKSPFDSSGGRH
TSFSSVFVYPEIDDSIEVEINPADLRIDTYRASGAGGQHINKTDSAVRITHMPTGIVVQCQNDRSQHRNRAEAMAMLKSR
LYEVEMRKRQAEQDKLESSKTDVGWGHQIRSYVLDQSRVKDLRTNVEMSNTRAVLDGDLDDFISASLKQGV

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=249, Percent_Identity=39.7590361445783, Blast_Score=174, Evalue=2e-43,
Organism=Homo sapiens, GI34577120, Length=252, Percent_Identity=38.4920634920635, Blast_Score=155, Evalue=4e-38,
Organism=Homo sapiens, GI166795305, Length=149, Percent_Identity=41.6107382550336, Blast_Score=123, Evalue=4e-28,
Organism=Escherichia coli, GI2367172, Length=343, Percent_Identity=63.5568513119534, Blast_Score=470, Evalue=1e-134,
Organism=Escherichia coli, GI1787462, Length=246, Percent_Identity=43.9024390243902, Blast_Score=213, Evalue=1e-56,
Organism=Caenorhabditis elegans, GI17542784, Length=239, Percent_Identity=34.7280334728033, Blast_Score=114, Evalue=9e-26,
Organism=Saccharomyces cerevisiae, GI6321295, Length=249, Percent_Identity=34.5381526104418, Blast_Score=148, Evalue=2e-36,
Organism=Drosophila melanogaster, GI19921226, Length=304, Percent_Identity=36.8421052631579, Blast_Score=166, Evalue=3e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 43594; Mature: 43594

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHRATLPIGYNSAFLRSKNSERSWKRNVSTRSKAPCSTCAPARASFGGIFDYDAKAARL
CCCCEECCCCCCHHHHCCCCCCHHHHHCCCCCCCCCCCCCCCCHHCCCCEECCCCHHHHH
TEVNKELEDPNVWNDSKNAQALGREKKLLEGVVTTLTALDSDLRDALDLFELAREEGDED
HHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
TLVASEEDAAKLASRVGDIEFRRMFSNPADPNNCFIDIQAGAGGTEACDWASMLLRQYLR
CEEECCHHHHHHHHHHCCHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHH
YCERKGFKAEVLEESDGDVAGIKNATVKVSGEYAYGFLRTETGIHRLVRKSPFDSSGGRH
HHHHCCCEEEECCCCCCCEECCCCCEEEEECCEEEEEEEHHHHHHHHHHCCCCCCCCCCC
TSFSSVFVYPEIDDSIEVEINPADLRIDTYRASGAGGQHINKTDSAVRITHMPTGIVVQC
CCCCEEEEEECCCCCEEEEECCCCEEEEEEECCCCCCCCCCCCCCEEEEEECCCCEEEEE
QNDRSQHRNRAEAMAMLKSRLYEVEMRKRQAEQDKLESSKTDVGWGHQIRSYVLDQSRVK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
DLRTNVEMSNTRAVLDGDLDDFISASLKQGV
HHHHCCCCCCCEEEECCCHHHHHHHHHHHCC
>Mature Secondary Structure
MRHRATLPIGYNSAFLRSKNSERSWKRNVSTRSKAPCSTCAPARASFGGIFDYDAKAARL
CCCCEECCCCCCHHHHCCCCCCHHHHHCCCCCCCCCCCCCCCCHHCCCCEECCCCHHHHH
TEVNKELEDPNVWNDSKNAQALGREKKLLEGVVTTLTALDSDLRDALDLFELAREEGDED
HHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
TLVASEEDAAKLASRVGDIEFRRMFSNPADPNNCFIDIQAGAGGTEACDWASMLLRQYLR
CEEECCHHHHHHHHHHCCHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHH
YCERKGFKAEVLEESDGDVAGIKNATVKVSGEYAYGFLRTETGIHRLVRKSPFDSSGGRH
HHHHCCCEEEECCCCCCCEECCCCCEEEEECCEEEEEEEHHHHHHHHHHCCCCCCCCCCC
TSFSSVFVYPEIDDSIEVEINPADLRIDTYRASGAGGQHINKTDSAVRITHMPTGIVVQC
CCCCEEEEEECCCCCEEEEECCCCEEEEEEECCCCCCCCCCCCCCEEEEEECCCCEEEEE
QNDRSQHRNRAEAMAMLKSRLYEVEMRKRQAEQDKLESSKTDVGWGHQIRSYVLDQSRVK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
DLRTNVEMSNTRAVLDGDLDDFISASLKQGV
HHHHCCCCCCCEEEECCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA