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The map label for this gene is lysS

Identifier: 78066893

GI number: 78066893

Start: 2493938

End: 2495512

Strand: Direct

Name: lysS

Synonym: Bcep18194_A5424

Alternate gene names: 78066893

Gene position: 2493938-2495512 (Clockwise)

Preceding gene: 78066892

Following gene: 78066894

Centisome position: 67.51

GC content: 64.7

Gene sequence:

>1575_bases
TTGCCCTTCCCGTTTGCGTTGCCGCACCCACTCCGAATTCCGACCATCATGACCGAACCGACCCAAACGCAGCCCGCCGT
CACCGCGGACGAAAACCAGATCATCGCCGAGCGCCGCGAGAAGCTGCGCGCCCTGCGCGAGCAAGGCGTCGCCTACCCGA
ACGATTTCCGCCCGACGCATCACGCAGCCGATCTGCAGACGACCTTCGCCGACTCGGACAAGGCCGCGCTGGAAGCGAAC
CCGGTCGAGGTGTCGGTTGCCGGCCGGATGATGCTCAAGCGCGTGATGGGCAAGGCAAGCTTCGCGACGGTGCAGGACGG
TTCGGGCCAGATCCAGTTCTTCGTGACGCCGAACGACGTCGGCGCGGAAACCTACGACGCATTCAAGAAGTGGGACCTCG
GCGACATCGTCGCCGCGCGCGGCGTGCTGTTCCGCACCAACAAGGGCGAGCTGTCGGTCCAGTGCAAGGAACTGCGCCTG
CTGTCGAAGGCGCTGCGTCCGCTGCCGGACAAGTTCCACGGCCTGTCGGACCAGGAAATGCGCTACCGCCAGCGCTACGT
CGACCTGATCGTCACGCCGGAAACGCGTGATACGTTCCGCGCCCGCACCAAGACGATCGCGTCGATCCGCAAGTTCATGG
ACAACGCCGAGTTCATGGAAGTCGAGACGCCGATGCTGCACCCGATCCCGGGCGGCGCGGCCGCGAAGCCGTTCGTCACG
CATCACAACGCGCTCGACATGCAGATGTTCCTGCGCATCGCACCGGAGCTGTACCTGAAGCGCCTGATCGTCGGCGGCTT
CGAACGCGTGTTCGAAATCAACCGCAACTTCCGGAATGAAGGCGTGTCGCCGCGTCACAACCCGGAATTCACGATGATGG
AGTTCTACGCCGCGTACACCGACTATCGCTGGCTGATGGACTTCACCGAGCAACTGATCCGCCAGGCGGCGATCGATGCG
CTCGGCACCGCGACGATCCAGTACCAGGGCCGCGAGCTCGACCTCGCGAAGCCGTTCCACCGCCTGACGATCACGCAGGC
GATCCAGAAGTACGCGCCGGACTACACCGCCGGCCAGCTGTCGGACGACGCGTTCCTGCGCACCGAACTGAAGCGCTTCG
GCGTCGACGTATCGCAGCCGGCGTTCCTGAACGCGGGCATCGGCGCGCTGCAACTCGCGCTGTTCGAGGAAACGGCCGAA
GCGCAGCTGTGGGAGCCGACCTTCATCATCGACTACCCGGTCGAAGTGTCGCCGCTCGCACGTGCATCGGATACGGTGCC
GGGCATCACCGAGCGTTTCGAGCTGTTCATGACCGGCCGCGAAATCGCGAACGGCTTCTCGGAGCTGAACGATCCGGAAG
ACCAGGCTGCGCGCTTCAAGAAGCAGGTCGAGCAGAAGGATGCGGGCGACGAGGAAGCGATGTTCTTCGACGCCGACTAC
ATCCGCGCACTCGAATACGGGATGCCTCCGACCGGCGGCTGCGGGATCGGCATCGACCGTCTCGTGATGCTGCTGACCGA
CAGCCCGACGATCCGCGACGTGCTGCTGTTCCCGCATCTGCGCCGCGAAGACTGA

Upstream 100 bases:

>100_bases
ACGCGCGCCGTCCTCGACGGCGACCTCGACGACTTCATCAGCGCGAGCCTCAAACAGGGCGTGTAAGCGCCGCGGCGCGA
CCCTGCCGGGTCGCGCCCCC

Downstream 100 bases:

>100_bases
TCGCCGCGCCCGCGTGTGCGCCGCGCCCTGCGGCGCGCACGCATCAGCTTTGTAACCACGCGTAAATCCCGCCAGCCGGC
GTCCGCCGGCTTTCGACCCC

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS [H]

Number of amino acids: Translated: 524; Mature: 523

Protein sequence:

>524_residues
MPFPFALPHPLRIPTIMTEPTQTQPAVTADENQIIAERREKLRALREQGVAYPNDFRPTHHAADLQTTFADSDKAALEAN
PVEVSVAGRMMLKRVMGKASFATVQDGSGQIQFFVTPNDVGAETYDAFKKWDLGDIVAARGVLFRTNKGELSVQCKELRL
LSKALRPLPDKFHGLSDQEMRYRQRYVDLIVTPETRDTFRARTKTIASIRKFMDNAEFMEVETPMLHPIPGGAAAKPFVT
HHNALDMQMFLRIAPELYLKRLIVGGFERVFEINRNFRNEGVSPRHNPEFTMMEFYAAYTDYRWLMDFTEQLIRQAAIDA
LGTATIQYQGRELDLAKPFHRLTITQAIQKYAPDYTAGQLSDDAFLRTELKRFGVDVSQPAFLNAGIGALQLALFEETAE
AQLWEPTFIIDYPVEVSPLARASDTVPGITERFELFMTGREIANGFSELNDPEDQAARFKKQVEQKDAGDEEAMFFDADY
IRALEYGMPPTGGCGIGIDRLVMLLTDSPTIRDVLLFPHLRRED

Sequences:

>Translated_524_residues
MPFPFALPHPLRIPTIMTEPTQTQPAVTADENQIIAERREKLRALREQGVAYPNDFRPTHHAADLQTTFADSDKAALEAN
PVEVSVAGRMMLKRVMGKASFATVQDGSGQIQFFVTPNDVGAETYDAFKKWDLGDIVAARGVLFRTNKGELSVQCKELRL
LSKALRPLPDKFHGLSDQEMRYRQRYVDLIVTPETRDTFRARTKTIASIRKFMDNAEFMEVETPMLHPIPGGAAAKPFVT
HHNALDMQMFLRIAPELYLKRLIVGGFERVFEINRNFRNEGVSPRHNPEFTMMEFYAAYTDYRWLMDFTEQLIRQAAIDA
LGTATIQYQGRELDLAKPFHRLTITQAIQKYAPDYTAGQLSDDAFLRTELKRFGVDVSQPAFLNAGIGALQLALFEETAE
AQLWEPTFIIDYPVEVSPLARASDTVPGITERFELFMTGREIANGFSELNDPEDQAARFKKQVEQKDAGDEEAMFFDADY
IRALEYGMPPTGGCGIGIDRLVMLLTDSPTIRDVLLFPHLRRED
>Mature_523_residues
PFPFALPHPLRIPTIMTEPTQTQPAVTADENQIIAERREKLRALREQGVAYPNDFRPTHHAADLQTTFADSDKAALEANP
VEVSVAGRMMLKRVMGKASFATVQDGSGQIQFFVTPNDVGAETYDAFKKWDLGDIVAARGVLFRTNKGELSVQCKELRLL
SKALRPLPDKFHGLSDQEMRYRQRYVDLIVTPETRDTFRARTKTIASIRKFMDNAEFMEVETPMLHPIPGGAAAKPFVTH
HNALDMQMFLRIAPELYLKRLIVGGFERVFEINRNFRNEGVSPRHNPEFTMMEFYAAYTDYRWLMDFTEQLIRQAAIDAL
GTATIQYQGRELDLAKPFHRLTITQAIQKYAPDYTAGQLSDDAFLRTELKRFGVDVSQPAFLNAGIGALQLALFEETAEA
QLWEPTFIIDYPVEVSPLARASDTVPGITERFELFMTGREIANGFSELNDPEDQAARFKKQVEQKDAGDEEAMFFDADYI
RALEYGMPPTGGCGIGIDRLVMLLTDSPTIRDVLLFPHLRRED

Specific function: Unknown

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI5031815, Length=514, Percent_Identity=39.8832684824903, Blast_Score=353, Evalue=2e-97,
Organism=Homo sapiens, GI194272210, Length=516, Percent_Identity=39.922480620155, Blast_Score=352, Evalue=3e-97,
Organism=Homo sapiens, GI40789249, Length=330, Percent_Identity=24.2424242424242, Blast_Score=81, Evalue=3e-15,
Organism=Escherichia coli, GI1789256, Length=506, Percent_Identity=58.300395256917, Blast_Score=605, Evalue=1e-174,
Organism=Escherichia coli, GI1790571, Length=506, Percent_Identity=58.102766798419, Blast_Score=601, Evalue=1e-173,
Organism=Escherichia coli, GI87082379, Length=328, Percent_Identity=31.7073170731707, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1788173, Length=281, Percent_Identity=24.9110320284698, Blast_Score=85, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17535925, Length=531, Percent_Identity=41.4312617702448, Blast_Score=365, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI17535927, Length=531, Percent_Identity=41.4312617702448, Blast_Score=365, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI71994340, Length=498, Percent_Identity=42.7710843373494, Blast_Score=363, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI17551876, Length=475, Percent_Identity=22.9473684210526, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI32566633, Length=282, Percent_Identity=24.822695035461, Blast_Score=82, Evalue=9e-16,
Organism=Saccharomyces cerevisiae, GI6320242, Length=519, Percent_Identity=41.6184971098266, Blast_Score=365, Evalue=1e-102,
Organism=Saccharomyces cerevisiae, GI6324256, Length=551, Percent_Identity=30.8529945553539, Blast_Score=196, Evalue=1e-50,
Organism=Saccharomyces cerevisiae, GI6321807, Length=455, Percent_Identity=24.1758241758242, Blast_Score=80, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6325153, Length=325, Percent_Identity=23.6923076923077, Blast_Score=63, Evalue=9e-11,
Organism=Drosophila melanogaster, GI24640849, Length=518, Percent_Identity=40.7335907335907, Blast_Score=367, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24640851, Length=518, Percent_Identity=40.7335907335907, Blast_Score=367, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24584738, Length=343, Percent_Identity=25.3644314868805, Blast_Score=89, Evalue=1e-17,

Paralogues:

None

Copy number: 1200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.6 [H]

Molecular weight: Translated: 59346; Mature: 59215

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFPFALPHPLRIPTIMTEPTQTQPAVTADENQIIAERREKLRALREQGVAYPNDFRPTH
CCCCCCCCCCCCCCEEECCCCCCCCCEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
HAADLQTTFADSDKAALEANPVEVSVAGRMMLKRVMGKASFATVQDGSGQIQFFVTPNDV
CCCCCHHHHCCCCCCEEECCCEEEEHHHHHHHHHHHCCCCEEEEECCCCEEEEEECCCCC
GAETYDAFKKWDLGDIVAARGVLFRTNKGELSVQCKELRLLSKALRPLPDKFHGLSDQEM
CCHHHHHHHCCCHHHHHHHCCEEEECCCCCEEEHHHHHHHHHHHHHCCCHHHCCCCHHHH
RYRQRYVDLIVTPETRDTFRARTKTIASIRKFMDNAEFMEVETPMLHPIPGGAAAKPFVT
HHHHHHEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCEEE
HHNALDMQMFLRIAPELYLKRLIVGGFERVFEINRNFRNEGVSPRHNPEFTMMEFYAAYT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
DYRWLMDFTEQLIRQAAIDALGTATIQYQGRELDLAKPFHRLTITQAIQKYAPDYTAGQL
HHHHHHHHHHHHHHHHHHHHHCCEEEEECCCEECCCCHHHHHHHHHHHHHHCCCCCCCCC
SDDAFLRTELKRFGVDVSQPAFLNAGIGALQLALFEETAEAQLWEPTFIIDYPVEVSPLA
CCHHHHHHHHHHHCCCCCCCCHHHCCHHHHHHHHHHHHHHCEECCCEEEEECCCCCCCCC
RASDTVPGITERFELFMTGREIANGFSELNDPEDQAARFKKQVEQKDAGDEEAMFFDADY
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEECHHH
IRALEYGMPPTGGCGIGIDRLVMLLTDSPTIRDVLLFPHLRRED
HHHHHCCCCCCCCCCCCHHHHEEEECCCCCHHHHHHCCCCCCCC
>Mature Secondary Structure 
PFPFALPHPLRIPTIMTEPTQTQPAVTADENQIIAERREKLRALREQGVAYPNDFRPTH
CCCCCCCCCCCCCEEECCCCCCCCCEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
HAADLQTTFADSDKAALEANPVEVSVAGRMMLKRVMGKASFATVQDGSGQIQFFVTPNDV
CCCCCHHHHCCCCCCEEECCCEEEEHHHHHHHHHHHCCCCEEEEECCCCEEEEEECCCCC
GAETYDAFKKWDLGDIVAARGVLFRTNKGELSVQCKELRLLSKALRPLPDKFHGLSDQEM
CCHHHHHHHCCCHHHHHHHCCEEEECCCCCEEEHHHHHHHHHHHHHCCCHHHCCCCHHHH
RYRQRYVDLIVTPETRDTFRARTKTIASIRKFMDNAEFMEVETPMLHPIPGGAAAKPFVT
HHHHHHEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCEEE
HHNALDMQMFLRIAPELYLKRLIVGGFERVFEINRNFRNEGVSPRHNPEFTMMEFYAAYT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
DYRWLMDFTEQLIRQAAIDALGTATIQYQGRELDLAKPFHRLTITQAIQKYAPDYTAGQL
HHHHHHHHHHHHHHHHHHHHHCCEEEEECCCEECCCCHHHHHHHHHHHHHHCCCCCCCCC
SDDAFLRTELKRFGVDVSQPAFLNAGIGALQLALFEETAEAQLWEPTFIIDYPVEVSPLA
CCHHHHHHHHHHHCCCCCCCCHHHCCHHHHHHHHHHHHHHCEECCCEEEEECCCCCCCCC
RASDTVPGITERFELFMTGREIANGFSELNDPEDQAARFKKQVEQKDAGDEEAMFFDADY
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEECHHH
IRALEYGMPPTGGCGIGIDRLVMLLTDSPTIRDVLLFPHLRRED
HHHHHCCCCCCCCCCCCHHHHEEEECCCCCHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA