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The map label for this gene is pyrB

Identifier: 78065439

GI number: 78065439

Start: 885882

End: 887027

Strand: Direct

Name: pyrB

Synonym: Bcep18194_A3965

Alternate gene names: 78065439

Gene position: 885882-887027 (Clockwise)

Preceding gene: 78065438

Following gene: 78065440

Centisome position: 23.98

GC content: 66.23

Gene sequence:

>1146_bases
ATGCGCAGTTCACGCTGCGCGTCGAGGCGCACGGCGCCTGAACGACACGCGAACCGTATCCCGGGCCGCTGGTTTGCACC
GCGGCCCGTTTGCATAGTTGGAATCACGCACACCATGACCACCGACACCACTGGCCGCACCGGCAATCCCGCTGCGACCG
CGAGCCCCGACCGGTTCCGCTACGGCTTCCTGAAGGGCAACCCGCAGCTCACGAAAAACGGCGAGCTGAAGCACCTGCTG
TCGATCGAGGGCCTGCCGCGCTCGATCGTCAACCACATCCTCGATACGGCCGAGCAGTTCGTCAGCGTGACGGACCGTGA
AGTGAAGAAAGTGCCGCTGCTGCGCGGCAAGTCCGTGTTCAACCTGTTCTTCGAGAACTCGACGCGCACGCGCACGACCT
TCGAGATCGCGGCGACGCGCCTGTCGGCCGACGTGCTGAACCTGAACATCAACGCATCGTCGACGAGCAAGGGCGAATCG
CTGCTCGACACGATCAACAACCTGTCGGCGATGCATGCCGACCTGTTCGTCGTGCGTCACGCGTCGAGCGGCGCACCGTA
CCTGATCGCCGAGCACTGCGCACCGCACGTGCACGTGATCAACGCCGGCGACGGCCGCCATGCGCACCCGACGCAGGGCC
TGCTCGACATGTACACGATCCGTCACTACAAGCGCGACTTCACGAAGCTGCGCGTGGCGATCGTCGGCGACATCCTGCAT
TCGCGCGTCGCGCGCTCCGACATTCACGCGCTCACCACGCTCGGCGTGCCGGAAGTGCGCGCGATCGGCCCGCGCACGCT
GCTGCCGGGTGGCCTCGAGCAGATGGGCGTGAAGGTGTTCCACAACCTCGACGAAGGGCTGAAGGGCGTCGACGTGATCA
TCATGCTGCGCCTGCAGAACGAGCGGATGAGCGGCGCGCTGCTGCCGTCGGCGCAGGAATACTTCAAGACCTGGGGCCTG
ACGCCCGAGCGCCTCGCGCTCGCCGCGCCCGACGCGATCGTGATGCACCCGGGCCCGATGAACCGCGGCGTCGAGATCGA
TTCGCAGGTCGCCGATGGCCCGCAGTCGGTGATCCTCAACCAGGTCACGTTCGGTATCGCCGTGCGGATGGCGGTGATGG
GCATCGTCGCCGGCAACAGCGACTGA

Upstream 100 bases:

>100_bases
CTCGCCGATCGCGGCGGCCGCGAGCTGCCGGTCGCCGCGCGTTTCGCCGGCGGCGCGCTCGACGTGCCGGCCGACGCGAC
GCTCGTGCTCGCACGCGACG

Downstream 100 bases:

>100_bases
GCCCGCTTTCGCGCATCCTTATTCACGCAATCAACGCATTCACAGACAGCGCATGAAGATTCATATCAAAGGCGGCACGC
TGATCGACCCGGTCGCCGGC

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase [H]

Number of amino acids: Translated: 381; Mature: 381

Protein sequence:

>381_residues
MRSSRCASRRTAPERHANRIPGRWFAPRPVCIVGITHTMTTDTTGRTGNPAATASPDRFRYGFLKGNPQLTKNGELKHLL
SIEGLPRSIVNHILDTAEQFVSVTDREVKKVPLLRGKSVFNLFFENSTRTRTTFEIAATRLSADVLNLNINASSTSKGES
LLDTINNLSAMHADLFVVRHASSGAPYLIAEHCAPHVHVINAGDGRHAHPTQGLLDMYTIRHYKRDFTKLRVAIVGDILH
SRVARSDIHALTTLGVPEVRAIGPRTLLPGGLEQMGVKVFHNLDEGLKGVDVIIMLRLQNERMSGALLPSAQEYFKTWGL
TPERLALAAPDAIVMHPGPMNRGVEIDSQVADGPQSVILNQVTFGIAVRMAVMGIVAGNSD

Sequences:

>Translated_381_residues
MRSSRCASRRTAPERHANRIPGRWFAPRPVCIVGITHTMTTDTTGRTGNPAATASPDRFRYGFLKGNPQLTKNGELKHLL
SIEGLPRSIVNHILDTAEQFVSVTDREVKKVPLLRGKSVFNLFFENSTRTRTTFEIAATRLSADVLNLNINASSTSKGES
LLDTINNLSAMHADLFVVRHASSGAPYLIAEHCAPHVHVINAGDGRHAHPTQGLLDMYTIRHYKRDFTKLRVAIVGDILH
SRVARSDIHALTTLGVPEVRAIGPRTLLPGGLEQMGVKVFHNLDEGLKGVDVIIMLRLQNERMSGALLPSAQEYFKTWGL
TPERLALAAPDAIVMHPGPMNRGVEIDSQVADGPQSVILNQVTFGIAVRMAVMGIVAGNSD
>Mature_381_residues
MRSSRCASRRTAPERHANRIPGRWFAPRPVCIVGITHTMTTDTTGRTGNPAATASPDRFRYGFLKGNPQLTKNGELKHLL
SIEGLPRSIVNHILDTAEQFVSVTDREVKKVPLLRGKSVFNLFFENSTRTRTTFEIAATRLSADVLNLNINASSTSKGES
LLDTINNLSAMHADLFVVRHASSGAPYLIAEHCAPHVHVINAGDGRHAHPTQGLLDMYTIRHYKRDFTKLRVAIVGDILH
SRVARSDIHALTTLGVPEVRAIGPRTLLPGGLEQMGVKVFHNLDEGLKGVDVIIMLRLQNERMSGALLPSAQEYFKTWGL
TPERLALAAPDAIVMHPGPMNRGVEIDSQVADGPQSVILNQVTFGIAVRMAVMGIVAGNSD

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=312, Percent_Identity=34.9358974358974, Blast_Score=162, Evalue=4e-40,
Organism=Escherichia coli, GI2367364, Length=309, Percent_Identity=33.3333333333333, Blast_Score=135, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI193204318, Length=333, Percent_Identity=32.1321321321321, Blast_Score=125, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6322331, Length=313, Percent_Identity=31.629392971246, Blast_Score=132, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24642586, Length=314, Percent_Identity=34.3949044585987, Blast_Score=144, Evalue=1e-34,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.2 [H]

Molecular weight: Translated: 41643; Mature: 41643

Theoretical pI: Translated: 10.17; Mature: 10.17

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSSRCASRRTAPERHANRIPGRWFAPRPVCIVGITHTMTTDTTGRTGNPAATASPDRFR
CCCCCCCHHCCCCHHHHHCCCCCCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCCCCEE
YGFLKGNPQLTKNGELKHLLSIEGLPRSIVNHILDTAEQFVSVTDREVKKVPLLRGKSVF
EEEEECCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEE
NLFFENSTRTRTTFEIAATRLSADVLNLNINASSTSKGESLLDTINNLSAMHADLFVVRH
EEEECCCCCCEEEHHHHHHHHEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHEEEEEEE
ASSGAPYLIAEHCAPHVHVINAGDGRHAHPTQGLLDMYTIRHYKRDFTKLRVAIVGDILH
CCCCCCEEECCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SRVARSDIHALTTLGVPEVRAIGPRTLLPGGLEQMGVKVFHNLDEGLKGVDVIIMLRLQN
HHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEEECC
ERMSGALLPSAQEYFKTWGLTPERLALAAPDAIVMHPGPMNRGVEIDSQVADGPQSVILN
CCCCCCCCCCHHHHHHHHCCCHHHEEEECCCEEEECCCCCCCCCEECCHHCCCHHHHHHH
QVTFGIAVRMAVMGIVAGNSD
HHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MRSSRCASRRTAPERHANRIPGRWFAPRPVCIVGITHTMTTDTTGRTGNPAATASPDRFR
CCCCCCCHHCCCCHHHHHCCCCCCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCCCCEE
YGFLKGNPQLTKNGELKHLLSIEGLPRSIVNHILDTAEQFVSVTDREVKKVPLLRGKSVF
EEEEECCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEE
NLFFENSTRTRTTFEIAATRLSADVLNLNINASSTSKGESLLDTINNLSAMHADLFVVRH
EEEECCCCCCEEEHHHHHHHHEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHEEEEEEE
ASSGAPYLIAEHCAPHVHVINAGDGRHAHPTQGLLDMYTIRHYKRDFTKLRVAIVGDILH
CCCCCCEEECCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SRVARSDIHALTTLGVPEVRAIGPRTLLPGGLEQMGVKVFHNLDEGLKGVDVIIMLRLQN
HHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEEECC
ERMSGALLPSAQEYFKTWGLTPERLALAAPDAIVMHPGPMNRGVEIDSQVADGPQSVILN
CCCCCCCCCCHHHHHHHHCCCHHHEEEECCCEEEECCCCCCCCCEECCHHCCCHHHHHHH
QVTFGIAVRMAVMGIVAGNSD
HHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA