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The map label for this gene is pyrC' [H]

Identifier: 78065440

GI number: 78065440

Start: 887080

End: 888357

Strand: Direct

Name: pyrC' [H]

Synonym: Bcep18194_A3966

Alternate gene names: 78065440

Gene position: 887080-888357 (Clockwise)

Preceding gene: 78065439

Following gene: 78065441

Centisome position: 24.01

GC content: 70.81

Gene sequence:

>1278_bases
ATGAAGATTCATATCAAAGGCGGCACGCTGATCGACCCGGTCGCCGGCACCGAGCAGCAGGCCGACGTATTCGTCGCAGG
CGGCACGATCGCCGCGCTCGGCACGGCGCCGGCCGGTTTCAACGCGGACAAGACGATCGACGCCGCGGGCCTCATCGTCG
CACCCGGCCTCGTCGACCTGTGCGCGCGGCTGCGCGAGCCCGGCTACGAGCATAAGGCGACGCTCGCGTCCGAGATGGCC
GCAGCCGTCGCGGGCGGCGTCACGACCCTCGTGTGCCCGCCGGATACCGACCCCGTGCTCGACGAGCCGGGCCTCGTCGA
GATGCTCAAGTACCGCGCACGCAACCTGCACCAGGCGAACGTGCATCCGCTCGGCGCGCTGACGGTCGGCCTCAAGGGTG
AAGTGATCACCGAGATGGTCGCGCTGACCGAGTCCGGCTGCGTCGGCTTCACCCATGCCAACGTGCCGGTGCGCGACACG
CAGGTGCTGCTGCGTGCGCTGCAGTACGCGAGCACCTACGACTACACCACGTGGCTGCGCCCGCAAGACGCATTCATCGG
CCGCGGCGGCGTCGCCGCGAGCGGCGCGCTGGCGTCGCGGCTCGGGCTGTCCGGCGTGCCGGTCGCGGCCGAGACGATCG
CGCTGCACACGATTTTCGAACTGATGCGCGTGACGGGCGCACGCGTGCACCTCGCGCGGCTGTCGTCGTCGGCCGGGCTC
GCGCTCGTGCGCGAGGCGAAGGCCGAGGGGCTGCCCGTGACCTGCGACGTCGGCGTGAATCACCTGCATCTGATCGACGT
CGACATCGGCTACTTCGATTCGCAGTTCCGGCTCGATCCGCCGCTGCGCAGCGAGCGCGATCGCGAAGCGATTCGCGTGG
CGCTCGCCGACGGCACGATCGACGCGATCTGCTCCGACCACACGCCGGTCGACGACGACGAGAAGCTGCTGCCGTTCGGC
GAAGCGACGCCGGGCGCGACGGGGCTCGAACTGCTGCTGTCGCTGACGCTGAAGTGGGCCGACGAGACGCGTACGCCGCT
TGCGCAGGCGTTGCGCCGCATCACGTCCGCACCGGCCGACGTGCTGAAGCTGCCGGCGGGCCGCCTCACCGAAGGCGGCG
CGGCCGACCTGTGCGTGTTCGACCCGCGCGCACACTGGCGCGTCGAGCCGCGTGCGCTGAAGAGCCAGGGCCACAACTCG
CCGTTCCTCGGCTACGAACTGCCCGCCAGCGTGCGCACGACGCTCGTCGCCGGGCAGATCGCGTTCGAGCGCTCCTGA

Upstream 100 bases:

>100_bases
GCCGTGCGGATGGCGGTGATGGGCATCGTCGCCGGCAACAGCGACTGAGCCCGCTTTCGCGCATCCTTATTCACGCAATC
AACGCATTCACAGACAGCGC

Downstream 100 bases:

>100_bases
ACCACGCCGGACCTTCGCCATGATTGCCCTTCGCAAGCTGCGTCTCGTTTTCCACCTGTTGCGCGGCATGGCGATCGTCG
CGCTGCGTTTCTCGCACGTC

Product: dihydroorotase

Products: Allantoate; H+ [C]

Alternate protein names: Aspartate carbamoyltransferase 44 kDa Non-catalytic chain [H]

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MKIHIKGGTLIDPVAGTEQQADVFVAGGTIAALGTAPAGFNADKTIDAAGLIVAPGLVDLCARLREPGYEHKATLASEMA
AAVAGGVTTLVCPPDTDPVLDEPGLVEMLKYRARNLHQANVHPLGALTVGLKGEVITEMVALTESGCVGFTHANVPVRDT
QVLLRALQYASTYDYTTWLRPQDAFIGRGGVAASGALASRLGLSGVPVAAETIALHTIFELMRVTGARVHLARLSSSAGL
ALVREAKAEGLPVTCDVGVNHLHLIDVDIGYFDSQFRLDPPLRSERDREAIRVALADGTIDAICSDHTPVDDDEKLLPFG
EATPGATGLELLLSLTLKWADETRTPLAQALRRITSAPADVLKLPAGRLTEGGAADLCVFDPRAHWRVEPRALKSQGHNS
PFLGYELPASVRTTLVAGQIAFERS

Sequences:

>Translated_425_residues
MKIHIKGGTLIDPVAGTEQQADVFVAGGTIAALGTAPAGFNADKTIDAAGLIVAPGLVDLCARLREPGYEHKATLASEMA
AAVAGGVTTLVCPPDTDPVLDEPGLVEMLKYRARNLHQANVHPLGALTVGLKGEVITEMVALTESGCVGFTHANVPVRDT
QVLLRALQYASTYDYTTWLRPQDAFIGRGGVAASGALASRLGLSGVPVAAETIALHTIFELMRVTGARVHLARLSSSAGL
ALVREAKAEGLPVTCDVGVNHLHLIDVDIGYFDSQFRLDPPLRSERDREAIRVALADGTIDAICSDHTPVDDDEKLLPFG
EATPGATGLELLLSLTLKWADETRTPLAQALRRITSAPADVLKLPAGRLTEGGAADLCVFDPRAHWRVEPRALKSQGHNS
PFLGYELPASVRTTLVAGQIAFERS
>Mature_425_residues
MKIHIKGGTLIDPVAGTEQQADVFVAGGTIAALGTAPAGFNADKTIDAAGLIVAPGLVDLCARLREPGYEHKATLASEMA
AAVAGGVTTLVCPPDTDPVLDEPGLVEMLKYRARNLHQANVHPLGALTVGLKGEVITEMVALTESGCVGFTHANVPVRDT
QVLLRALQYASTYDYTTWLRPQDAFIGRGGVAASGALASRLGLSGVPVAAETIALHTIFELMRVTGARVHLARLSSSAGL
ALVREAKAEGLPVTCDVGVNHLHLIDVDIGYFDSQFRLDPPLRSERDREAIRVALADGTIDAICSDHTPVDDDEKLLPFG
EATPGATGLELLLSLTLKWADETRTPLAQALRRITSAPADVLKLPAGRLTEGGAADLCVFDPRAHWRVEPRALKSQGHNS
PFLGYELPASVRTTLVAGQIAFERS

Specific function: Non-functional DHOase [H]

COG id: COG0044

COG function: function code F; Dihydroorotase and related cyclic amidohydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHOase family. PyrC' subfamily [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=397, Percent_Identity=27.2040302267003, Blast_Score=111, Evalue=2e-24,
Organism=Escherichia coli, GI1786722, Length=444, Percent_Identity=26.3513513513513, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI87082175, Length=441, Percent_Identity=25.3968253968254, Blast_Score=97, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI193204318, Length=403, Percent_Identity=25.0620347394541, Blast_Score=98, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6322218, Length=438, Percent_Identity=23.972602739726, Blast_Score=88, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24642586, Length=376, Percent_Identity=26.8617021276596, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI18859883, Length=210, Percent_Identity=30.4761904761905, Blast_Score=85, Evalue=9e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006680
- InterPro:   IPR004722
- InterPro:   IPR011059 [H]

Pfam domain/function: PF01979 Amidohydro_1 [H]

EC number: 3.5.2.5 [C]

Molecular weight: Translated: 44898; Mature: 44898

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIHIKGGTLIDPVAGTEQQADVFVAGGTIAALGTAPAGFNADKTIDAAGLIVAPGLVDL
CEEEECCCCEECCCCCCCCCCCEEEECCEEEEECCCCCCCCCCCCCCCCCEEECCCHHHH
CARLREPGYEHKATLASEMAAAVAGGVTTLVCPPDTDPVLDEPGLVEMLKYRARNLHQAN
HHHHHCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCC
VHPLGALTVGLKGEVITEMVALTESGCVGFTHANVPVRDTQVLLRALQYASTYDYTTWLR
CCCCEEEEECCCHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCEEECC
PQDAFIGRGGVAASGALASRLGLSGVPVAAETIALHTIFELMRVTGARVHLARLSSSAGL
CCHHHCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCE
ALVREAKAEGLPVTCDVGVNHLHLIDVDIGYFDSQFRLDPPLRSERDREAIRVALADGTI
EEEEHHHCCCCCEEEECCCCEEEEEEEECCCCCCCEEECCCCCCCCCCCEEEEEEECCCE
DAICSDHTPVDDDEKLLPFGEATPGATGLELLLSLTLKWADETRTPLAQALRRITSAPAD
EEHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHH
VLKLPAGRLTEGGAADLCVFDPRAHWRVEPRALKSQGHNSPFLGYELPASVRTTLVAGQI
HHHCCCCCCCCCCCCCEEEECCCCCEEECHHHHHCCCCCCCCCCEECCHHHHHHEEEEEE
AFERS
EECCC
>Mature Secondary Structure
MKIHIKGGTLIDPVAGTEQQADVFVAGGTIAALGTAPAGFNADKTIDAAGLIVAPGLVDL
CEEEECCCCEECCCCCCCCCCCEEEECCEEEEECCCCCCCCCCCCCCCCCEEECCCHHHH
CARLREPGYEHKATLASEMAAAVAGGVTTLVCPPDTDPVLDEPGLVEMLKYRARNLHQAN
HHHHHCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCC
VHPLGALTVGLKGEVITEMVALTESGCVGFTHANVPVRDTQVLLRALQYASTYDYTTWLR
CCCCEEEEECCCHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCEEECC
PQDAFIGRGGVAASGALASRLGLSGVPVAAETIALHTIFELMRVTGARVHLARLSSSAGL
CCHHHCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCE
ALVREAKAEGLPVTCDVGVNHLHLIDVDIGYFDSQFRLDPPLRSERDREAIRVALADGTI
EEEEHHHCCCCCEEEECCCCEEEEEEEECCCCCCCEEECCCCCCCCCCCEEEEEEECCCE
DAICSDHTPVDDDEKLLPFGEATPGATGLELLLSLTLKWADETRTPLAQALRRITSAPAD
EEHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHH
VLKLPAGRLTEGGAADLCVFDPRAHWRVEPRALKSQGHNSPFLGYELPASVRTTLVAGQI
HHHCCCCCCCCCCCCCEEEECCCCCEEECHHHHHCCCCCCCCCCEECCHHHHHHEEEEEE
AFERS
EECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Allantoin; H2O [C]

Specific reaction: Allantoin + H2O --> Allantoate + H+ [C]

General reaction: Carboxylic acid amide hydrolysis [C]

Inhibitor: Dithiothreitol; p-Chloromercuribenzene-sulphonic acid [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]