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The map label for this gene is tyrS [H]

Identifier: 78065245

GI number: 78065245

Start: 664401

End: 665642

Strand: Direct

Name: tyrS [H]

Synonym: Bcep18194_A3769

Alternate gene names: 78065245

Gene position: 664401-665642 (Clockwise)

Preceding gene: 78065243

Following gene: 78065246

Centisome position: 17.99

GC content: 65.62

Gene sequence:

>1242_bases
ATGAGCACCGAACCCAGTTCCAAGCCTGTTTTCCCGATCACCGACGAAGTCCGGCATGCGCTCGCCGTCACGAAGCGCGG
CGTCGACGAGCTGCTGGTCGAGGAAGAGTTCGCGCAGAAGCTCGCGCGCAGCGCGGCGACGGGCACGCCGCTTCGCATCA
AGCTCGGCCTCGACCCGACGGCGCCTGACATCCACATCGGCCATACGGTCGTGCTGAACAAGATGCGTCAGCTGCAGGAC
CTCGGCCACACGGTGATCTTCCTGATCGGCGATTTCACGTCGCTGATCGGCGACCCGTCGGGCCGCAACGCGACGCGCCC
GCCGCTCACGCGCGAGCAGATCGAGTCGAACGCGAAGACCTACTTCGAGCAGGCCGCACTCGTGCTCGACCGCGACAAGA
CCGAAATCCGCTACAACAGCGAATGGTCGATGCCGCTCGGCGCGGACGGGATGATCAAGCTCGCGTCGCGCTACACGGTC
GCGCGGATCCTCGAGCGCGAGGATTTCACGAAGCGCTTCCAGGGCGGCGTGCCGATCTCGATCCACGAATTCCTGTACCC
GCTGATGCAGGGCTACGACTCGGTCGCGCTGAACGCCGACCTCGAGCTCGGCGGCACCGACCAGAAGTTCAACCTGCTGG
TCGGCCGCGAACTGCAGAAGCAGTACGGCCAGGAACAGCAGTGCATTCTGACGATGCCGCTGCTCGAAGGTCTCGACGGC
GTCGAGAAGATGTCGAAGTCGAAGGGCAACTACGTCGGCATCAGCGAGCAGCCGACCGACATGTTCGGCAAGCTGATGAG
CATCTCGGACACGCTGATGTGGCGGTACTTCGAACTGCTGTCGTTCCGCCCGATGGACGAGATCGGCGGCTTCAAGCGCG
AGATCGAAGGCGGCCGCAACCCGCGCGACTTCAAGGTGCTGCTCGCGCAGGAAATCGTCGCGCGCTTCCACTCGCAAGCC
GACGCCGAGCGTGCGCTCGAAGATTTCAACCACCGCGCGAAGGGCGGCGTGCCGGACGACATTCCGTCGGTCACGCTCGC
GGGCGCGCCGCTGGCGATCGGCCAGTTGCTGAAGCAGGCCGGCCTCGTGCCGTCGACGAGCGAGGCGCTGCGCAACATCG
AGCAGGGCGGCGTGAAGATCGATGGCGCGACCGTGTCGGACAAGGGCCTGAAGGTCGAAGCCGGCGAGTTCGTCGTGCAG
GTCGGCAAGCGCCGCTTCGCGCGCGTCACGCTGACCGCATGA

Upstream 100 bases:

>100_bases
GCCTTGCGCCCGATTATCCGCGCAACGTGACGCGCCGTTAAGCGATGGGCGCGCAAGTTATGGGAAAATCCCTGTCTTTG
CGACATTCTTCCTGGCACCG

Downstream 100 bases:

>100_bases
TCGCGCTGATCCAGCGCGTGAAGCGCGCCGACGTGCGCGTCGGCGACCGTACGACCGGCGAGATCGGCGCGGGCCTGCTC
GCGCTGGTCTGCGCGGAGCG

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS [H]

Number of amino acids: Translated: 413; Mature: 412

Protein sequence:

>413_residues
MSTEPSSKPVFPITDEVRHALAVTKRGVDELLVEEEFAQKLARSAATGTPLRIKLGLDPTAPDIHIGHTVVLNKMRQLQD
LGHTVIFLIGDFTSLIGDPSGRNATRPPLTREQIESNAKTYFEQAALVLDRDKTEIRYNSEWSMPLGADGMIKLASRYTV
ARILEREDFTKRFQGGVPISIHEFLYPLMQGYDSVALNADLELGGTDQKFNLLVGRELQKQYGQEQQCILTMPLLEGLDG
VEKMSKSKGNYVGISEQPTDMFGKLMSISDTLMWRYFELLSFRPMDEIGGFKREIEGGRNPRDFKVLLAQEIVARFHSQA
DAERALEDFNHRAKGGVPDDIPSVTLAGAPLAIGQLLKQAGLVPSTSEALRNIEQGGVKIDGATVSDKGLKVEAGEFVVQ
VGKRRFARVTLTA

Sequences:

>Translated_413_residues
MSTEPSSKPVFPITDEVRHALAVTKRGVDELLVEEEFAQKLARSAATGTPLRIKLGLDPTAPDIHIGHTVVLNKMRQLQD
LGHTVIFLIGDFTSLIGDPSGRNATRPPLTREQIESNAKTYFEQAALVLDRDKTEIRYNSEWSMPLGADGMIKLASRYTV
ARILEREDFTKRFQGGVPISIHEFLYPLMQGYDSVALNADLELGGTDQKFNLLVGRELQKQYGQEQQCILTMPLLEGLDG
VEKMSKSKGNYVGISEQPTDMFGKLMSISDTLMWRYFELLSFRPMDEIGGFKREIEGGRNPRDFKVLLAQEIVARFHSQA
DAERALEDFNHRAKGGVPDDIPSVTLAGAPLAIGQLLKQAGLVPSTSEALRNIEQGGVKIDGATVSDKGLKVEAGEFVVQ
VGKRRFARVTLTA
>Mature_412_residues
STEPSSKPVFPITDEVRHALAVTKRGVDELLVEEEFAQKLARSAATGTPLRIKLGLDPTAPDIHIGHTVVLNKMRQLQDL
GHTVIFLIGDFTSLIGDPSGRNATRPPLTREQIESNAKTYFEQAALVLDRDKTEIRYNSEWSMPLGADGMIKLASRYTVA
RILEREDFTKRFQGGVPISIHEFLYPLMQGYDSVALNADLELGGTDQKFNLLVGRELQKQYGQEQQCILTMPLLEGLDGV
EKMSKSKGNYVGISEQPTDMFGKLMSISDTLMWRYFELLSFRPMDEIGGFKREIEGGRNPRDFKVLLAQEIVARFHSQAD
AERALEDFNHRAKGGVPDDIPSVTLAGAPLAIGQLLKQAGLVPSTSEALRNIEQGGVKIDGATVSDKGLKVEAGEFVVQV
GKRRFARVTLTA

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=398, Percent_Identity=25.8793969849246, Blast_Score=102, Evalue=5e-22,
Organism=Escherichia coli, GI1787925, Length=424, Percent_Identity=29.0094339622642, Blast_Score=134, Evalue=7e-33,
Organism=Caenorhabditis elegans, GI25145802, Length=210, Percent_Identity=29.0476190476191, Blast_Score=82, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6325160, Length=383, Percent_Identity=25.8485639686684, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI20130383, Length=450, Percent_Identity=25.3333333333333, Blast_Score=127, Evalue=1e-29,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 45658; Mature: 45527

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS50889 S4 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTEPSSKPVFPITDEVRHALAVTKRGVDELLVEEEFAQKLARSAATGTPLRIKLGLDPT
CCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCC
APDIHIGHTVVLNKMRQLQDLGHTVIFLIGDFTSLIGDPSGRNATRPPLTREQIESNAKT
CCCCCCCHHHHHHHHHHHHHCCCEEEEHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
YFEQAALVLDRDKTEIRYNSEWSMPLGADGMIKLASRYTVARILEREDFTKRFQGGVPIS
HHHHHHHHEECCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
IHEFLYPLMQGYDSVALNADLELGGTDQKFNLLVGRELQKQYGQEQQCILTMPLLEGLDG
HHHHHHHHHCCCCCEEEECCEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEHHHHHCCHH
VEKMSKSKGNYVGISEQPTDMFGKLMSISDTLMWRYFELLSFRPMDEIGGFKREIEGGRN
HHHHHHCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCHHHHHCCCCC
PRDFKVLLAQEIVARFHSQADAERALEDFNHRAKGGVPDDIPSVTLAGAPLAIGQLLKQA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCHHHHHHHHHHC
GLVPSTSEALRNIEQGGVKIDGATVSDKGLKVEAGEFVVQVGKRRFARVTLTA
CCCCCHHHHHHHHHHCCEEECCCEECCCCCEEEHHHHHHHHHHHHEEEEEECC
>Mature Secondary Structure 
STEPSSKPVFPITDEVRHALAVTKRGVDELLVEEEFAQKLARSAATGTPLRIKLGLDPT
CCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCC
APDIHIGHTVVLNKMRQLQDLGHTVIFLIGDFTSLIGDPSGRNATRPPLTREQIESNAKT
CCCCCCCHHHHHHHHHHHHHCCCEEEEHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
YFEQAALVLDRDKTEIRYNSEWSMPLGADGMIKLASRYTVARILEREDFTKRFQGGVPIS
HHHHHHHHEECCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
IHEFLYPLMQGYDSVALNADLELGGTDQKFNLLVGRELQKQYGQEQQCILTMPLLEGLDG
HHHHHHHHHCCCCCEEEECCEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEHHHHHCCHH
VEKMSKSKGNYVGISEQPTDMFGKLMSISDTLMWRYFELLSFRPMDEIGGFKREIEGGRN
HHHHHHCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCHHHHHCCCCC
PRDFKVLLAQEIVARFHSQADAERALEDFNHRAKGGVPDDIPSVTLAGAPLAIGQLLKQA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCHHHHHHHHHHC
GLVPSTSEALRNIEQGGVKIDGATVSDKGLKVEAGEFVVQVGKRRFARVTLTA
CCCCCHHHHHHHHHHCCEEECCCEECCCCCEEEHHHHHHHHHHHHEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA