Click here to switch to the map view.

The map label for this gene is leuS

Identifier: 78065216

GI number: 78065216

Start: 629348

End: 631942

Strand: Direct

Name: leuS

Synonym: Bcep18194_A3740

Alternate gene names: 78065216

Gene position: 629348-631942 (Clockwise)

Preceding gene: 78065215

Following gene: 78065217

Centisome position: 17.04

GC content: 65.86

Gene sequence:

>2595_bases
ATGCACGAGAGATACGTACCCGCCGACGTCGAAGCCGCCGCCCAGGGCGACTGGCGCGCAGCCGATGCCTACAAGACGCA
GGAAGATTCGCAGAAGCCGAAGTTCTACTGCGTGTCGATGCTGCCGTACCCGTCCGGCAAGCTGCACATGGGTCACGTGC
GCAACTACACGATCAACGACGTGATGTACCGCTATCTGCGGATGAACGGCTACAACACGCTGATGCCGATGGGTTGGGAT
GCGTTCGGGATGCCGGCCGAGAACGCCGCGATGGCGAACGGCGTGCCGCCCGCGAAGTGGACCTACGACAACATCGACTA
CATGAAGGGCCAGATGCAGTCGATGGGCCTCGCGATCGACTGGTCGCGCGAAATCGCGACGTGCAAGCCCGACTACTACA
AGTGGAACCAGTGGCTGTTCCTGAAGATGCTCGAGAAGGGCATCGCGTACAAGAAGACGGGCACCGTGAACTGGGACCCG
GTCGACCAGACCGTGCTCGCGAACGAGCAGGTGATCGACGGCCGCGGCTGGCGGTCGGGCGCGCTCGTCGAGAAGCGCGA
AATCCCGATGTACTACCTGCGCATCACGCAGTACGCGGATGAGCTGCTGAACGACCTCGACGGCCTCGGCTGGCCCGAGC
GCGTGAAGATCATGCAGCAGAACTGGATCGGCAAGAGCTTCGGCGTGAACTTCGGCTTCCCGTACGAACTCGACGGCGAG
AAGAAGCTGCTGCGCGTGTTCACGACGCGCGCCGACACGATCATGGGCGTGACCTTCTGCGCGATCGCGGCCGAGCATCC
GCTCGCCACGCGCCTCGCGCAGGACAAGCCGGAACTGCTCACGTTCATCGACGAATGCAAGCGCGGCGGCGTCGCCGAGG
CCGACGTTGCGACGATGGAGAAGAAGGGCGTCGCGACGGGCTTCTCGGTCGCACACCCGCTGACCGGCGAGCCGGTCGAG
GTGTGGATCGGCAACTACGTGCTGATGAGCTATGGCGAAGGCGCGGTGATGGGCGTGCCGGGCCACGACGAGCGCGATTT
CGCGTTCGCGAAGAAATACGACCTGCCGATCAAGCAGGTGATCTCGGCCGAAGGCCAGCAGTACTCGCTCGACGCATGGC
AGGAGTGGTACGGCGACAAGGAAACCGCGGTCTGCGTGAACAGCGGCAAGTACGACGGCCTGCGCTACGCGGAAGCCGTC
GACGCGGTCGCGGCCGACCTGAACGCCGGCGGCTTCGGCGACAAGCAGGTCACGTGGCGCCTGCGCGACTGGGGCGTATC
GCGCCAGCGCTACTGGGGCACGCCGATCCCGATCATCCACTGCCCGTCGTGCGGCGACGTGCCGGTGCCGGAGCAGGATC
TCCCCGTCGTGCTGCCGGAAGACCTCGTGCCGGACGGCTCGGGCAACCCGCTCGCGAAGTCGGAAGCGTTCCTGAACTGC
GCGTGCCCGAAGTGCGGCGCGGCAGCCAAGCGCGAAACCGACACGATGGATACCTTCGTCGATTCGTCGTGGTACTTCTC
GCGCTACACGGCGCCGGACGCCGAGACGATGGTCGATGCGCGCACCGATTACTGGATGCCGATGGATCAGTACATCGGCG
GCATCGAGCACGCGATCCTGCACTTGCTGTATTCGCGTTTCTGGACCAAGGTGATGCGCGACCTCGGCCTCGTGAAGTTC
GGCGAGCCGGCGAAGAACCTGCTCACGCAGGGCATGGTGCTGAACGAGACGTTCTACCGCGAGGATGCATCGGGCAAGAA
GACCTGGTACAACCCGCTCGACGTGACCGTCACGCACGACGACAAGGGCCGCCCGGTCGGCGCGACGCTGAACACGGACG
GCCAGCCGGTCGTGCTCGGCGGCATCGAGAAGATGTCGAAGTCGAAGAACAACGGCGTCGATCCGCAGCTGTTGATCGAC
CAGTACGGCGCCGATACCGCGCGCCTGTTCACGATGTTCGCTGCACCGCCCGAGCAGCAGCTCGAGTGGTCGGGTGCGGG
CGTCGAGGGCGCAAGCCGCTTCCTGCGCCGCGTGTGGAGCTTCGGTGCGGCGAACCGCGAAGCGCTCGCCGCGCGCGCGG
GCTTCGATGCGGCCGCGCTCGGCGAAGCCGACAAGGCGCTGCGCCGCGAGATCTACAGCGTGCTGAAGCAGGCCGATTTC
GACTACCAGCGCCTGCAGTACAACACGGTGGTGTCGGCTGCGATGAAGATGCTGAACGCGATCGACGGCGCGAAGGGTGC
GACGCCCGGCGTGCTGCGTGAAACGTACGGTGTGCTGCTGCGCGTGCTGTACCCGGTCGTGCCGCACGTCACGTTCGAGC
TGTGGAAGGCGCTGGGCTACGCGGACGAATTCGGCCCGCTGCTCGACGCACCGTGGCCGAAGGTCGACGAGGCCGCGCTC
GAGCAGGCCGAGATCGAACTCGTGCTGCAGGTGAACGGCAAGGTGCGCGGCGCGCTGAAGGTGGCAAAGGACGCGAGCCG
CGAGGCGATCGAAGCCGCGGCAGTGGCCGACGACGCGTTCGCGAAGTTCAGCGACGGCAAGCCGGCGAAGAAGATCGTCG
TCGTGCCGGGCCGCCTCGTGAACATCGTCGTCTGA

Upstream 100 bases:

>100_bases
CGGCAACAGCGAACCTGCAGGTCCGCCGGGCAAGCACGATATTCGATCCACTCCTGCGCGCGCGCCGTCGCGCCGCTTAA
AGCCTAGTCCGAACCACACC

Downstream 100 bases:

>100_bases
CGGCCGCTGGCCGTCGGACGTACCCAGAAGGAGCGAAGGTGATCCGCAGATCGTTTTTGATGCTCGCTGTCGGCAGCGCG
GTTGCGCTGTCGGCATGCGG

Product: leucyl-tRNA synthetase

Products: NA

Alternate protein names: Leucine--tRNA ligase; LeuRS [H]

Number of amino acids: Translated: 864; Mature: 864

Protein sequence:

>864_residues
MHERYVPADVEAAAQGDWRAADAYKTQEDSQKPKFYCVSMLPYPSGKLHMGHVRNYTINDVMYRYLRMNGYNTLMPMGWD
AFGMPAENAAMANGVPPAKWTYDNIDYMKGQMQSMGLAIDWSREIATCKPDYYKWNQWLFLKMLEKGIAYKKTGTVNWDP
VDQTVLANEQVIDGRGWRSGALVEKREIPMYYLRITQYADELLNDLDGLGWPERVKIMQQNWIGKSFGVNFGFPYELDGE
KKLLRVFTTRADTIMGVTFCAIAAEHPLATRLAQDKPELLTFIDECKRGGVAEADVATMEKKGVATGFSVAHPLTGEPVE
VWIGNYVLMSYGEGAVMGVPGHDERDFAFAKKYDLPIKQVISAEGQQYSLDAWQEWYGDKETAVCVNSGKYDGLRYAEAV
DAVAADLNAGGFGDKQVTWRLRDWGVSRQRYWGTPIPIIHCPSCGDVPVPEQDLPVVLPEDLVPDGSGNPLAKSEAFLNC
ACPKCGAAAKRETDTMDTFVDSSWYFSRYTAPDAETMVDARTDYWMPMDQYIGGIEHAILHLLYSRFWTKVMRDLGLVKF
GEPAKNLLTQGMVLNETFYREDASGKKTWYNPLDVTVTHDDKGRPVGATLNTDGQPVVLGGIEKMSKSKNNGVDPQLLID
QYGADTARLFTMFAAPPEQQLEWSGAGVEGASRFLRRVWSFGAANREALAARAGFDAAALGEADKALRREIYSVLKQADF
DYQRLQYNTVVSAAMKMLNAIDGAKGATPGVLRETYGVLLRVLYPVVPHVTFELWKALGYADEFGPLLDAPWPKVDEAAL
EQAEIELVLQVNGKVRGALKVAKDASREAIEAAAVADDAFAKFSDGKPAKKIVVVPGRLVNIVV

Sequences:

>Translated_864_residues
MHERYVPADVEAAAQGDWRAADAYKTQEDSQKPKFYCVSMLPYPSGKLHMGHVRNYTINDVMYRYLRMNGYNTLMPMGWD
AFGMPAENAAMANGVPPAKWTYDNIDYMKGQMQSMGLAIDWSREIATCKPDYYKWNQWLFLKMLEKGIAYKKTGTVNWDP
VDQTVLANEQVIDGRGWRSGALVEKREIPMYYLRITQYADELLNDLDGLGWPERVKIMQQNWIGKSFGVNFGFPYELDGE
KKLLRVFTTRADTIMGVTFCAIAAEHPLATRLAQDKPELLTFIDECKRGGVAEADVATMEKKGVATGFSVAHPLTGEPVE
VWIGNYVLMSYGEGAVMGVPGHDERDFAFAKKYDLPIKQVISAEGQQYSLDAWQEWYGDKETAVCVNSGKYDGLRYAEAV
DAVAADLNAGGFGDKQVTWRLRDWGVSRQRYWGTPIPIIHCPSCGDVPVPEQDLPVVLPEDLVPDGSGNPLAKSEAFLNC
ACPKCGAAAKRETDTMDTFVDSSWYFSRYTAPDAETMVDARTDYWMPMDQYIGGIEHAILHLLYSRFWTKVMRDLGLVKF
GEPAKNLLTQGMVLNETFYREDASGKKTWYNPLDVTVTHDDKGRPVGATLNTDGQPVVLGGIEKMSKSKNNGVDPQLLID
QYGADTARLFTMFAAPPEQQLEWSGAGVEGASRFLRRVWSFGAANREALAARAGFDAAALGEADKALRREIYSVLKQADF
DYQRLQYNTVVSAAMKMLNAIDGAKGATPGVLRETYGVLLRVLYPVVPHVTFELWKALGYADEFGPLLDAPWPKVDEAAL
EQAEIELVLQVNGKVRGALKVAKDASREAIEAAAVADDAFAKFSDGKPAKKIVVVPGRLVNIVV
>Mature_864_residues
MHERYVPADVEAAAQGDWRAADAYKTQEDSQKPKFYCVSMLPYPSGKLHMGHVRNYTINDVMYRYLRMNGYNTLMPMGWD
AFGMPAENAAMANGVPPAKWTYDNIDYMKGQMQSMGLAIDWSREIATCKPDYYKWNQWLFLKMLEKGIAYKKTGTVNWDP
VDQTVLANEQVIDGRGWRSGALVEKREIPMYYLRITQYADELLNDLDGLGWPERVKIMQQNWIGKSFGVNFGFPYELDGE
KKLLRVFTTRADTIMGVTFCAIAAEHPLATRLAQDKPELLTFIDECKRGGVAEADVATMEKKGVATGFSVAHPLTGEPVE
VWIGNYVLMSYGEGAVMGVPGHDERDFAFAKKYDLPIKQVISAEGQQYSLDAWQEWYGDKETAVCVNSGKYDGLRYAEAV
DAVAADLNAGGFGDKQVTWRLRDWGVSRQRYWGTPIPIIHCPSCGDVPVPEQDLPVVLPEDLVPDGSGNPLAKSEAFLNC
ACPKCGAAAKRETDTMDTFVDSSWYFSRYTAPDAETMVDARTDYWMPMDQYIGGIEHAILHLLYSRFWTKVMRDLGLVKF
GEPAKNLLTQGMVLNETFYREDASGKKTWYNPLDVTVTHDDKGRPVGATLNTDGQPVVLGGIEKMSKSKNNGVDPQLLID
QYGADTARLFTMFAAPPEQQLEWSGAGVEGASRFLRRVWSFGAANREALAARAGFDAAALGEADKALRREIYSVLKQADF
DYQRLQYNTVVSAAMKMLNAIDGAKGATPGVLRETYGVLLRVLYPVVPHVTFELWKALGYADEFGPLLDAPWPKVDEAAL
EQAEIELVLQVNGKVRGALKVAKDASREAIEAAAVADDAFAKFSDGKPAKKIVVVPGRLVNIVV

Specific function: Unknown

COG id: COG0495

COG function: function code J; Leucyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI7661872, Length=868, Percent_Identity=36.6359447004608, Blast_Score=528, Evalue=1e-149,
Organism=Escherichia coli, GI1786861, Length=874, Percent_Identity=50, Blast_Score=865, Evalue=0.0,
Organism=Escherichia coli, GI1790708, Length=386, Percent_Identity=23.8341968911917, Blast_Score=84, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI71997510, Length=267, Percent_Identity=40.8239700374532, Blast_Score=220, Evalue=3e-57,
Organism=Caenorhabditis elegans, GI71997517, Length=267, Percent_Identity=40.8239700374532, Blast_Score=220, Evalue=3e-57,
Organism=Caenorhabditis elegans, GI212645227, Length=338, Percent_Identity=30.1775147928994, Blast_Score=142, Evalue=7e-34,
Organism=Saccharomyces cerevisiae, GI6323414, Length=835, Percent_Identity=40.1197604790419, Blast_Score=559, Evalue=1e-160,
Organism=Saccharomyces cerevisiae, GI6321531, Length=401, Percent_Identity=23.4413965087282, Blast_Score=92, Evalue=4e-19,
Organism=Drosophila melanogaster, GI21355409, Length=897, Percent_Identity=35.4515050167224, Blast_Score=454, Evalue=1e-127,
Organism=Drosophila melanogaster, GI281366294, Length=367, Percent_Identity=21.7983651226158, Blast_Score=68, Evalue=3e-11,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002300
- InterPro:   IPR002302
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155
- InterPro:   IPR009008 [H]

Pfam domain/function: PF08264 Anticodon_1; PF00133 tRNA-synt_1 [H]

EC number: =6.1.1.4 [H]

Molecular weight: Translated: 96090; Mature: 96090

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHERYVPADVEAAAQGDWRAADAYKTQEDSQKPKFYCVSMLPYPSGKLHMGHVRNYTIND
CCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCEEECCCCCCCHHH
VMYRYLRMNGYNTLMPMGWDAFGMPAENAAMANGVPPAKWTYDNIDYMKGQMQSMGLAID
HHHHHHHHCCCCEECCCCCCCCCCCCCCCHHCCCCCCCCEECCCHHHHHHHHHHCCEEEE
WSREIATCKPDYYKWNQWLFLKMLEKGIAYKKTGTVNWDPVDQTVLANEQVIDGRGWRSG
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEECCCCCCCCCCCHHHCCCEEECCCCCCCC
ALVEKREIPMYYLRITQYADELLNDLDGLGWPERVKIMQQNWIGKSFGVNFGFPYELDGE
CCEECCCCCEEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHCCCCCCCCEEECCH
KKLLRVFTTRADTIMGVTFCAIAAEHPLATRLAQDKPELLTFIDECKRGGVAEADVATME
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHH
KKGVATGFSVAHPLTGEPVEVWIGNYVLMSYGEGAVMGVPGHDERDFAFAKKYDLPIKQV
HCCCCCCCCCCCCCCCCCCEEEECCEEEEECCCCCEEECCCCCCCCCHHHHHCCCCHHHH
ISAEGQQYSLDAWQEWYGDKETAVCVNSGKYDGLRYAEAVDAVAADLNAGGFGDKQVTWR
HHCCCCEEEHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEE
LRDWGVSRQRYWGTPIPIIHCPSCGDVPVPEQDLPVVLPEDLVPDGSGNPLAKSEAFLNC
EHHCCCCCHHCCCCCCCEEECCCCCCCCCCCCCCCEECCHHCCCCCCCCCCCCCCHHEEE
ACPKCGAAAKRETDTMDTFVDSSWYFSRYTAPDAETMVDARTDYWMPMDQYIGGIEHAIL
CCCCCCCCCCCCCCHHHHHHCCCCEEEEECCCCHHHHHHCCCCCCCCHHHHHCHHHHHHH
HLLYSRFWTKVMRDLGLVKFGEPAKNLLTQGMVLNETFYREDASGKKTWYNPLDVTVTHD
HHHHHHHHHHHHHHCCCEECCCHHHHHHHCCCCCCHHHHCCCCCCCCCCCCCEEEEEEEC
DKGRPVGATLNTDGQPVVLGGIEKMSKSKNNGVDPQLLIDQYGADTARLFTMFAAPPEQQ
CCCCEECEEECCCCCEEEEECHHHHHCCCCCCCCHHHHHHHCCCCHHHHHHEECCCCHHH
LEWSGAGVEGASRFLRRVWSFGAANREALAARAGFDAAALGEADKALRREIYSVLKQADF
CEECCCCHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHCHHHHHHHHHHHHHHHHCCC
DYQRLQYNTVVSAAMKMLNAIDGAKGATPGVLRETYGVLLRVLYPVVPHVTFELWKALGY
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
ADEFGPLLDAPWPKVDEAALEQAEIELVLQVNGKVRGALKVAKDASREAIEAAAVADDAF
CHHHCCCCCCCCCCCCHHHHHHCCEEEEEEECCCEEHHHHHHHHHHHHHHHHHHHHHHHH
AKFSDGKPAKKIVVVPGRLVNIVV
HHCCCCCCCCEEEECCCCEEEEEC
>Mature Secondary Structure
MHERYVPADVEAAAQGDWRAADAYKTQEDSQKPKFYCVSMLPYPSGKLHMGHVRNYTIND
CCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCEEECCCCCCCHHH
VMYRYLRMNGYNTLMPMGWDAFGMPAENAAMANGVPPAKWTYDNIDYMKGQMQSMGLAID
HHHHHHHHCCCCEECCCCCCCCCCCCCCCHHCCCCCCCCEECCCHHHHHHHHHHCCEEEE
WSREIATCKPDYYKWNQWLFLKMLEKGIAYKKTGTVNWDPVDQTVLANEQVIDGRGWRSG
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEECCCCCCCCCCCHHHCCCEEECCCCCCCC
ALVEKREIPMYYLRITQYADELLNDLDGLGWPERVKIMQQNWIGKSFGVNFGFPYELDGE
CCEECCCCCEEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHCCCCCCCCEEECCH
KKLLRVFTTRADTIMGVTFCAIAAEHPLATRLAQDKPELLTFIDECKRGGVAEADVATME
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHH
KKGVATGFSVAHPLTGEPVEVWIGNYVLMSYGEGAVMGVPGHDERDFAFAKKYDLPIKQV
HCCCCCCCCCCCCCCCCCCEEEECCEEEEECCCCCEEECCCCCCCCCHHHHHCCCCHHHH
ISAEGQQYSLDAWQEWYGDKETAVCVNSGKYDGLRYAEAVDAVAADLNAGGFGDKQVTWR
HHCCCCEEEHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEE
LRDWGVSRQRYWGTPIPIIHCPSCGDVPVPEQDLPVVLPEDLVPDGSGNPLAKSEAFLNC
EHHCCCCCHHCCCCCCCEEECCCCCCCCCCCCCCCEECCHHCCCCCCCCCCCCCCHHEEE
ACPKCGAAAKRETDTMDTFVDSSWYFSRYTAPDAETMVDARTDYWMPMDQYIGGIEHAIL
CCCCCCCCCCCCCCHHHHHHCCCCEEEEECCCCHHHHHHCCCCCCCCHHHHHCHHHHHHH
HLLYSRFWTKVMRDLGLVKFGEPAKNLLTQGMVLNETFYREDASGKKTWYNPLDVTVTHD
HHHHHHHHHHHHHHCCCEECCCHHHHHHHCCCCCCHHHHCCCCCCCCCCCCCEEEEEEEC
DKGRPVGATLNTDGQPVVLGGIEKMSKSKNNGVDPQLLIDQYGADTARLFTMFAAPPEQQ
CCCCEECEEECCCCCEEEEECHHHHHCCCCCCCCHHHHHHHCCCCHHHHHHEECCCCHHH
LEWSGAGVEGASRFLRRVWSFGAANREALAARAGFDAAALGEADKALRREIYSVLKQADF
CEECCCCHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHCHHHHHHHHHHHHHHHHCCC
DYQRLQYNTVVSAAMKMLNAIDGAKGATPGVLRETYGVLLRVLYPVVPHVTFELWKALGY
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
ADEFGPLLDAPWPKVDEAALEQAEIELVLQVNGKVRGALKVAKDASREAIEAAAVADDAF
CHHHCCCCCCCCCCCCHHHHHHCCEEEEEEECCCEEHHHHHHHHHHHHHHHHHHHHHHHH
AKFSDGKPAKKIVVVPGRLVNIVV
HHCCCCCCCCEEEECCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA