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The map label for this gene is tgt [H]

Identifier: 78048251

GI number: 78048251

Start: 3051654

End: 3052799

Strand: Reverse

Name: tgt [H]

Synonym: XCV2695

Alternate gene names: 78048251

Gene position: 3052799-3051654 (Counterclockwise)

Preceding gene: 78048252

Following gene: 78048250

Centisome position: 58.95

GC content: 66.67

Gene sequence:

>1146_bases
ATGTCCCGACTCCAGTTCCAGCTCCAAGCCACCGACGGGCATGCCCGACGTGGGCGCCTGACCTTTCCGCGTGGCACGGT
CGAAACCCCTGCGTTCATGCCGGTCGGCACCTATGGCTCGGTCAAGGGCATCCTGCCCGAGCAGATCCGCGCGCTGGGTG
CGGAGATCATCCTGGGCAACACCTTCCACCTGTATCTGCGCCCGGGCCTGGAGGTCATCGGCGATCACGGCGGGCTGCAC
GGATTTGCGCGCTGGGATGGGCCCATCCTCACCGACTCCGGCGGGTTCCAGGTGTTTTCGCTGGCGCATCGCCGCAAGAT
CACCGAGCAGGGCGTGACCTTTTCCTCGCCGACCGATGGCGCGCGGGTGTTCCTGGGCCCCGAAGAGAGCATGAAAATTC
AAAAGGTGCTCGATTCGGACATCGTGATGATCTTCGACGAGTGCACGCCGTACCCGGCCACCGAGGACGTCGCGCGCCGC
TCGATGGAGCTGAGCCTGCGCTGGGCGCAGCGCTCGCGCGACGCGCACGACGGGCTGGGCAACGATGCGGCGCTGTTCGG
CATCGTGCAGGGTGGGGTGCATCCGGACCTACGCAGCCGCTCGCTGGATGGCCTGCAAGGCATCGGTTTCGACGGGTATG
CGATCGGCGGGCTGGCAGTGGGCGAGCCCGAGCACGAGCGCAACGCCATGCTCGAACACCTGCATCCGCGCCTGCCGGCC
GAGCGCCCGCGTTACCTGATGGGCGTGGGCCGCCCGGAAGACCTGGTCGAAGGCGTGGCACGCGGGGTGGACATGTTCGA
TTGCGTCATGCCCACCCGCAATGCGCGCAACGGCCACTATTTCACCTCGTTCGGCACCGTGCGCATCCGCAACGCAAAGT
ACGAACGCGACCTGGACACCATCGAGCCGGGCTGCGGCTGCCATGCCTGCAGCAGCGGCTATACGCGCGCCTACCTGCGC
CATCTGGACCGCTGCAACGAAATGCTGGCGCCGATGCTGGGCACCCTGCACAACCTCTGCTACTACGAAAAACTGATGGC
CGACATGCGTGCGGCGATCGCTTCGGGAACCTTTGTGGAGTTCCGGCGGTCCTTCTATGCGGCACGCGGCGCCACCACGC
CGCCGTTGCCGGGGGAAACCAGCTGA

Upstream 100 bases:

>100_bases
CGGGAATGGGGAATCGTAAGGCGGGACCCGCCGAACGATCAGCCACCTGCCAATCGCTCTTCCCATTCCCGATTCCCGAC
TCCCCATTCCCGGCCCCTCA

Downstream 100 bases:

>100_bases
GACCGGCCGTCCGCGCCCCCGGCGCGGACGGCGTGGAACCTGCGTGGCATAATTCCCGGCTGTTCCGCCCCGGCGGTACC
TGAATAGCGAGCGGGCCCAT

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 381; Mature: 380

Protein sequence:

>381_residues
MSRLQFQLQATDGHARRGRLTFPRGTVETPAFMPVGTYGSVKGILPEQIRALGAEIILGNTFHLYLRPGLEVIGDHGGLH
GFARWDGPILTDSGGFQVFSLAHRRKITEQGVTFSSPTDGARVFLGPEESMKIQKVLDSDIVMIFDECTPYPATEDVARR
SMELSLRWAQRSRDAHDGLGNDAALFGIVQGGVHPDLRSRSLDGLQGIGFDGYAIGGLAVGEPEHERNAMLEHLHPRLPA
ERPRYLMGVGRPEDLVEGVARGVDMFDCVMPTRNARNGHYFTSFGTVRIRNAKYERDLDTIEPGCGCHACSSGYTRAYLR
HLDRCNEMLAPMLGTLHNLCYYEKLMADMRAAIASGTFVEFRRSFYAARGATTPPLPGETS

Sequences:

>Translated_381_residues
MSRLQFQLQATDGHARRGRLTFPRGTVETPAFMPVGTYGSVKGILPEQIRALGAEIILGNTFHLYLRPGLEVIGDHGGLH
GFARWDGPILTDSGGFQVFSLAHRRKITEQGVTFSSPTDGARVFLGPEESMKIQKVLDSDIVMIFDECTPYPATEDVARR
SMELSLRWAQRSRDAHDGLGNDAALFGIVQGGVHPDLRSRSLDGLQGIGFDGYAIGGLAVGEPEHERNAMLEHLHPRLPA
ERPRYLMGVGRPEDLVEGVARGVDMFDCVMPTRNARNGHYFTSFGTVRIRNAKYERDLDTIEPGCGCHACSSGYTRAYLR
HLDRCNEMLAPMLGTLHNLCYYEKLMADMRAAIASGTFVEFRRSFYAARGATTPPLPGETS
>Mature_380_residues
SRLQFQLQATDGHARRGRLTFPRGTVETPAFMPVGTYGSVKGILPEQIRALGAEIILGNTFHLYLRPGLEVIGDHGGLHG
FARWDGPILTDSGGFQVFSLAHRRKITEQGVTFSSPTDGARVFLGPEESMKIQKVLDSDIVMIFDECTPYPATEDVARRS
MELSLRWAQRSRDAHDGLGNDAALFGIVQGGVHPDLRSRSLDGLQGIGFDGYAIGGLAVGEPEHERNAMLEHLHPRLPAE
RPRYLMGVGRPEDLVEGVARGVDMFDCVMPTRNARNGHYFTSFGTVRIRNAKYERDLDTIEPGCGCHACSSGYTRAYLRH
LDRCNEMLAPMLGTLHNLCYYEKLMADMRAAIASGTFVEFRRSFYAARGATTPPLPGETS

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=360, Percent_Identity=43.6111111111111, Blast_Score=290, Evalue=1e-78,
Organism=Escherichia coli, GI1786607, Length=373, Percent_Identity=64.0750670241287, Blast_Score=503, Evalue=1e-143,
Organism=Caenorhabditis elegans, GI17544680, Length=363, Percent_Identity=40.495867768595, Blast_Score=259, Evalue=1e-69,
Organism=Caenorhabditis elegans, GI25146502, Length=142, Percent_Identity=28.8732394366197, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI19920510, Length=370, Percent_Identity=42.4324324324324, Blast_Score=291, Evalue=5e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 42029; Mature: 41898

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLQFQLQATDGHARRGRLTFPRGTVETPAFMPVGTYGSVKGILPEQIRALGAEIILGN
CCCEEEEEEECCCCCCCCEEECCCCCCCCCCCEECCCCCCCCCCCHHHHHHCCCEEEECC
TFHLYLRPGLEVIGDHGGLHGFARWDGPILTDSGGFQVFSLAHRRKITEQGVTFSSPTDG
EEEEEECCCCCEECCCCCCCEEEECCCCEEECCCCCEEHHHHHHHHHHHCCCEECCCCCC
ARVFLGPEESMKIQKVLDSDIVMIFDECTPYPATEDVARRSMELSLRWAQRSRDAHDGLG
CEEEECCCHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCHHCCCC
NDAALFGIVQGGVHPDLRSRSLDGLQGIGFDGYAIGGLAVGEPEHERNAMLEHLHPRLPA
CCCEEEEEECCCCCCCHHHCCCCCCCCCCCCCEEECCEECCCCCHHHHHHHHHHCCCCCC
ERPRYLMGVGRPEDLVEGVARGVDMFDCVMPTRNARNGHYFTSFGTVRIRNAKYERDLDT
CCCCEEEECCCHHHHHHHHHCCCCHHHHHCCCCCCCCCEEEEEECEEEEECCCCCCCCCC
IEPGCGCHACSSGYTRAYLRHLDRCNEMLAPMLGTLHNLCYYEKLMADMRAAIASGTFVE
CCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
FRRSFYAARGATTPPLPGETS
HHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
SRLQFQLQATDGHARRGRLTFPRGTVETPAFMPVGTYGSVKGILPEQIRALGAEIILGN
CCEEEEEEECCCCCCCCEEECCCCCCCCCCCEECCCCCCCCCCCHHHHHHCCCEEEECC
TFHLYLRPGLEVIGDHGGLHGFARWDGPILTDSGGFQVFSLAHRRKITEQGVTFSSPTDG
EEEEEECCCCCEECCCCCCCEEEECCCCEEECCCCCEEHHHHHHHHHHHCCCEECCCCCC
ARVFLGPEESMKIQKVLDSDIVMIFDECTPYPATEDVARRSMELSLRWAQRSRDAHDGLG
CEEEECCCHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCHHCCCC
NDAALFGIVQGGVHPDLRSRSLDGLQGIGFDGYAIGGLAVGEPEHERNAMLEHLHPRLPA
CCCEEEEEECCCCCCCHHHCCCCCCCCCCCCCEEECCEECCCCCHHHHHHHHHHCCCCCC
ERPRYLMGVGRPEDLVEGVARGVDMFDCVMPTRNARNGHYFTSFGTVRIRNAKYERDLDT
CCCCEEEECCCHHHHHHHHHCCCCHHHHHCCCCCCCCCEEEEEECEEEEECCCCCCCCCC
IEPGCGCHACSSGYTRAYLRHLDRCNEMLAPMLGTLHNLCYYEKLMADMRAAIASGTFVE
CCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
FRRSFYAARGATTPPLPGETS
HHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]