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The map label for this gene is queA [H]

Identifier: 78048252

GI number: 78048252

Start: 3052881

End: 3053951

Strand: Reverse

Name: queA [H]

Synonym: XCV2696

Alternate gene names: 78048252

Gene position: 3053951-3052881 (Counterclockwise)

Preceding gene: 78048253

Following gene: 78048251

Centisome position: 58.97

GC content: 68.53

Gene sequence:

>1071_bases
TTGAAGAAGTCCGATTTCCATTACGAGCTGCCCGAAGAACTGATCGCCCAGGCGCCGCTGGCCGAACGTGCCGCCAGCCG
CCTGCTGGTGGTGCCGCCGGCGCCAGCGGCCTTCGGCGACCGCCAGGTGCGCGATCTGCCCGAGCTGCTGCAGCCCGGCG
ATCTGCTGATCTTCAACGACACCCGGGTCATTCCGGCGCGCTTGTTCGGGCAGAAGGCCAGCGGCGGGCGAGTGGAGATC
CTGATCGAGCGCCTGCTTGGCGAGCGCGAGGCGCGCGTACAGATCGGCGCCAGCAAGTCGCCCAAGGCCGGCAGCGTGAT
CGCGCTCGATGCCGGCGGCCAGGCCGAGGTGCTGGGCCGCGATGGCGAGTTCTATCTGCTGCGTTTCCAGATCCCGACCC
CGCTGGAGCACTGGCTGCTCGAGGCTGGCCGCCTGCCGCTGCCGCCCTATATCCGCCGCGAGCCGGGTGTCGAAGACCGC
GAGCGCTATCAGACCGTGTTCGCGCGCGAAGTCGGCGCGGTCGCCGCGCCCACCGCCGGGCTGCACTTCGACGAGCCGCT
GCTGGCGCGCCTGCGCGAGCGCGGGGTGGAGTTCGGCCACGTCACCTTGCATGTGGGCGCCGGCACCTTCCAGCCGGTGC
GGGTGGACAAGCTCGACCAGCACGTGATGCACAAGGAGTGGCTCAACGTCGGTGCCACGCTGGTCGAACAGGTACGCCGC
ACCCGCGCCCGCGGCGGCCGCGTGATCGCGGTGGGCACGACAGTGGTGCGCTCGCTGGAAAGCGCCTGGCGCAAGACCGA
GGCCGCGCCCGAAGGCGAATTGCAGCCATTTGCCGGCGAAACCCAGATCTTCATCCTGCCCGGCTACCGCATCCGCAGCG
TCGACGCGATGGTCACCAACTTCCATCTGCCCGAAAGCACCTTGATGATGATGGTCTGCGCCTTCGCCGGCCGCGAGCGC
ATCTTCGCCGCCTACCACCACGCCATCGCGCAGCGCTATCGCTTCTTTTCGTACGGCGATGCGATGTTGTTGTGGGGCGG
GGAATCGGGAGTCGGGAATGGGGAATCGTAA

Upstream 100 bases:

>100_bases
CGACGATGAAGCGCTGCCGCCCGTCCGATTCCCCATTCCCAATTCTCAATTCCCGGCCGCAGTTACACTATGCGGCTACT
CCGCCAACGATCCTGCGGCC

Downstream 100 bases:

>100_bases
GGCGGGACCCGCCGAACGATCAGCCACCTGCCAATCGCTCTTCCCATTCCCGATTCCCGACTCCCCATTCCCGGCCCCTC
AATGTCCCGACTCCAGTTCC

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA [H]

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MKKSDFHYELPEELIAQAPLAERAASRLLVVPPAPAAFGDRQVRDLPELLQPGDLLIFNDTRVIPARLFGQKASGGRVEI
LIERLLGEREARVQIGASKSPKAGSVIALDAGGQAEVLGRDGEFYLLRFQIPTPLEHWLLEAGRLPLPPYIRREPGVEDR
ERYQTVFAREVGAVAAPTAGLHFDEPLLARLRERGVEFGHVTLHVGAGTFQPVRVDKLDQHVMHKEWLNVGATLVEQVRR
TRARGGRVIAVGTTVVRSLESAWRKTEAAPEGELQPFAGETQIFILPGYRIRSVDAMVTNFHLPESTLMMMVCAFAGRER
IFAAYHHAIAQRYRFFSYGDAMLLWGGESGVGNGES

Sequences:

>Translated_356_residues
MKKSDFHYELPEELIAQAPLAERAASRLLVVPPAPAAFGDRQVRDLPELLQPGDLLIFNDTRVIPARLFGQKASGGRVEI
LIERLLGEREARVQIGASKSPKAGSVIALDAGGQAEVLGRDGEFYLLRFQIPTPLEHWLLEAGRLPLPPYIRREPGVEDR
ERYQTVFAREVGAVAAPTAGLHFDEPLLARLRERGVEFGHVTLHVGAGTFQPVRVDKLDQHVMHKEWLNVGATLVEQVRR
TRARGGRVIAVGTTVVRSLESAWRKTEAAPEGELQPFAGETQIFILPGYRIRSVDAMVTNFHLPESTLMMMVCAFAGRER
IFAAYHHAIAQRYRFFSYGDAMLLWGGESGVGNGES
>Mature_356_residues
MKKSDFHYELPEELIAQAPLAERAASRLLVVPPAPAAFGDRQVRDLPELLQPGDLLIFNDTRVIPARLFGQKASGGRVEI
LIERLLGEREARVQIGASKSPKAGSVIALDAGGQAEVLGRDGEFYLLRFQIPTPLEHWLLEAGRLPLPPYIRREPGVEDR
ERYQTVFAREVGAVAAPTAGLHFDEPLLARLRERGVEFGHVTLHVGAGTFQPVRVDKLDQHVMHKEWLNVGATLVEQVRR
TRARGGRVIAVGTTVVRSLESAWRKTEAAPEGELQPFAGETQIFILPGYRIRSVDAMVTNFHLPESTLMMMVCAFAGRER
IFAAYHHAIAQRYRFFSYGDAMLLWGGESGVGNGES

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA) [H]

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family [H]

Homologues:

Organism=Escherichia coli, GI1786606, Length=346, Percent_Identity=52.8901734104046, Blast_Score=359, Evalue=1e-100,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003699 [H]

Pfam domain/function: PF02547 Queuosine_synth [H]

EC number: 5.-.-.- [C]

Molecular weight: Translated: 39473; Mature: 39473

Theoretical pI: Translated: 7.39; Mature: 7.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKSDFHYELPEELIAQAPLAERAASRLLVVPPAPAAFGDRQVRDLPELLQPGDLLIFND
CCCCCCCCCCCHHHHHCCCHHHHHHCCEEEECCCCCCCCCCHHHHHHHHHCCCCEEEECC
TRVIPARLFGQKASGGRVEILIERLLGEREARVQIGASKSPKAGSVIALDAGGQAEVLGR
CCEEHHHHCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEECCCCCEEEEEC
DGEFYLLRFQIPTPLEHWLLEAGRLPLPPYIRREPGVEDRERYQTVFAREVGAVAAPTAG
CCCEEEEEEECCCCHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCC
LHFDEPLLARLRERGVEFGHVTLHVGAGTFQPVRVDKLDQHVMHKEWLNVGATLVEQVRR
CCCCCHHHHHHHHCCCCEEEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHH
TRARGGRVIAVGTTVVRSLESAWRKTEAAPEGELQPFAGETQIFILPGYRIRSVDAMVTN
HHHCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCEEEHHHHHHHC
FHLPESTLMMMVCAFAGRERIFAAYHHAIAQRYRFFSYGDAMLLWGGESGVGNGES
CCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHEECCCEEEEECCCCCCCCCCC
>Mature Secondary Structure
MKKSDFHYELPEELIAQAPLAERAASRLLVVPPAPAAFGDRQVRDLPELLQPGDLLIFND
CCCCCCCCCCCHHHHHCCCHHHHHHCCEEEECCCCCCCCCCHHHHHHHHHCCCCEEEECC
TRVIPARLFGQKASGGRVEILIERLLGEREARVQIGASKSPKAGSVIALDAGGQAEVLGR
CCEEHHHHCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEECCCCCEEEEEC
DGEFYLLRFQIPTPLEHWLLEAGRLPLPPYIRREPGVEDRERYQTVFAREVGAVAAPTAG
CCCEEEEEEECCCCHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCC
LHFDEPLLARLRERGVEFGHVTLHVGAGTFQPVRVDKLDQHVMHKEWLNVGATLVEQVRR
CCCCCHHHHHHHHCCCCEEEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHH
TRARGGRVIAVGTTVVRSLESAWRKTEAAPEGELQPFAGETQIFILPGYRIRSVDAMVTN
HHHCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCEEEHHHHHHHC
FHLPESTLMMMVCAFAGRERIFAAYHHAIAQRYRFFSYGDAMLLWGGESGVGNGES
CCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHEECCCEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]