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The map label for this gene is potF [H]

Identifier: 78048210

GI number: 78048210

Start: 2990617

End: 2991732

Strand: Reverse

Name: potF [H]

Synonym: XCV2654

Alternate gene names: 78048210

Gene position: 2991732-2990617 (Counterclockwise)

Preceding gene: 78048211

Following gene: 78048209

Centisome position: 57.77

GC content: 61.56

Gene sequence:

>1116_bases
ATGACGCTGCGACTGCTCACCCTGACGCTGTCCATCACCCTGTTGTCCGGCTGTGGCGGCGCTGGCGCGCCCGGCAAGAC
CGACGCGCAGGCCAAGGTGCTCAACGTCTACAACTATTCCGATTACATCGCCGAGGACACCATTCCCGGGTTCGAAAAGA
GCACCGGCATCAAGGTCACCTACGACGTCTTCGACAGCGACGAAATGGTGGAAACCAAGTTGCTGGCCGGCGGCAGCGAT
TACGACGTGGTGGTGCCCACGCTGAACTTCTTCGGCCGGCAGATCCAGGCCGGCGTCTTCCTGCCGCTGGACAAGAGCAA
GATCCCCAATCTGGTCAATCTGGATCCGGACGTGATGCGCCGCATCGCCGCGCAGGACCCGAACAATGCCTATGGCGTGC
CGTACATGATCGGCACCACCGGCATCGGCTACAACGTCGACAAGCTCAAGGCGATCTTCGGCAACACCGATGTCGCCAAC
AGCTGGGATCTGGTGTTCAAGCCGGAGAATCTGTCCAAACTCAAGGACTGCGGCGTGACCATCCTGGACACGCCTTCGGA
CATGATTCCGATTGCGCTCAATTATCTCGGGCTCGATCCGCACAGCACGGCGCCGGCCGAGATCGAAAAGGCCGCCGCGC
TGATCAAGGCCATTCGCCCGTACGTGCAGAACTTCCACTCCAGCCAGTACGTGACCTCGCTGGCCAACGGCAATACCTGC
CTGGCGGTGGGCTGGTCCGGCGACATCATCCAGGCGCGCGACCGTGCCGTGGAAGCCGGCAACAACATCAAGGTGGCTTA
CGCCATTCCGAAGGAAGGCGCGCCGCAGTGGTTCGACATGCTGGCCATTCCCAAGGATGCCAAGCACCCTGACAACGCCT
ACGCCTTCATCAATTATCTGTTGAAGTCGGACGTGGCCGCGGCCAACACCAACTTCATCCATTACGCCAACCCGGTGCGC
ACGGCCACGCCGTTGGTGGATGCGGCGATCCGCAACGACCCCACCATCTACCCGCCGCCGGAAGTGACGGCCAAGATGTT
TACCTACGCGATCAATCCGCCGGACGTGGACCGGCTGTACACGCGGCTGTGGACCGAGATCAAGACCGGGCATTGA

Upstream 100 bases:

>100_bases
TGCCGGTCCCGCTGCGCTGGGCAAACCCGGGCGTGGTGAGTAGGCTGGGAAGAGACCGGATTCGATACCGGCCGCCCCAT
CGCGCTGCCTGGAGACCCAC

Downstream 100 bases:

>100_bases
CCGGCTGCGTCGGCGGCGGGTGCTGCGCCCGAGCGCAGTGCGGCCCCGTGCGACGGCTCCTCCCACGGGCCACGCGCATG
GCGTGGGAATGGCACCGTCG

Product: ABC transporter putrescine-binding protein precursor

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 371; Mature: 370

Protein sequence:

>371_residues
MTLRLLTLTLSITLLSGCGGAGAPGKTDAQAKVLNVYNYSDYIAEDTIPGFEKSTGIKVTYDVFDSDEMVETKLLAGGSD
YDVVVPTLNFFGRQIQAGVFLPLDKSKIPNLVNLDPDVMRRIAAQDPNNAYGVPYMIGTTGIGYNVDKLKAIFGNTDVAN
SWDLVFKPENLSKLKDCGVTILDTPSDMIPIALNYLGLDPHSTAPAEIEKAAALIKAIRPYVQNFHSSQYVTSLANGNTC
LAVGWSGDIIQARDRAVEAGNNIKVAYAIPKEGAPQWFDMLAIPKDAKHPDNAYAFINYLLKSDVAAANTNFIHYANPVR
TATPLVDAAIRNDPTIYPPPEVTAKMFTYAINPPDVDRLYTRLWTEIKTGH

Sequences:

>Translated_371_residues
MTLRLLTLTLSITLLSGCGGAGAPGKTDAQAKVLNVYNYSDYIAEDTIPGFEKSTGIKVTYDVFDSDEMVETKLLAGGSD
YDVVVPTLNFFGRQIQAGVFLPLDKSKIPNLVNLDPDVMRRIAAQDPNNAYGVPYMIGTTGIGYNVDKLKAIFGNTDVAN
SWDLVFKPENLSKLKDCGVTILDTPSDMIPIALNYLGLDPHSTAPAEIEKAAALIKAIRPYVQNFHSSQYVTSLANGNTC
LAVGWSGDIIQARDRAVEAGNNIKVAYAIPKEGAPQWFDMLAIPKDAKHPDNAYAFINYLLKSDVAAANTNFIHYANPVR
TATPLVDAAIRNDPTIYPPPEVTAKMFTYAINPPDVDRLYTRLWTEIKTGH
>Mature_370_residues
TLRLLTLTLSITLLSGCGGAGAPGKTDAQAKVLNVYNYSDYIAEDTIPGFEKSTGIKVTYDVFDSDEMVETKLLAGGSDY
DVVVPTLNFFGRQIQAGVFLPLDKSKIPNLVNLDPDVMRRIAAQDPNNAYGVPYMIGTTGIGYNVDKLKAIFGNTDVANS
WDLVFKPENLSKLKDCGVTILDTPSDMIPIALNYLGLDPHSTAPAEIEKAAALIKAIRPYVQNFHSSQYVTSLANGNTCL
AVGWSGDIIQARDRAVEAGNNIKVAYAIPKEGAPQWFDMLAIPKDAKHPDNAYAFINYLLKSDVAAANTNFIHYANPVRT
ATPLVDAAIRNDPTIYPPPEVTAKMFTYAINPPDVDRLYTRLWTEIKTGH

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787078, Length=358, Percent_Identity=51.9553072625698, Blast_Score=399, Evalue=1e-112,
Organism=Escherichia coli, GI1787367, Length=340, Percent_Identity=33.2352941176471, Blast_Score=165, Evalue=6e-42,
Organism=Escherichia coli, GI1787711, Length=327, Percent_Identity=27.217125382263, Blast_Score=72, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 40425; Mature: 40294

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLRLLTLTLSITLLSGCGGAGAPGKTDAQAKVLNVYNYSDYIAEDTIPGFEKSTGIKVT
CEEEEEEEEEEEHHHCCCCCCCCCCCCCCCEEEEEEECCHHHHHHCCCCCCCCCCCCEEE
YDVFDSDEMVETKLLAGGSDYDVVVPTLNFFGRQIQAGVFLPLDKSKIPNLVNLDPDVMR
EEECCCCHHHHHHHCCCCCCCEEEECCHHHCCHHHCCCEEEECCHHHCCCCCCCCHHHHH
RIAAQDPNNAYGVPYMIGTTGIGYNVDKLKAIFGNTDVANSWDLVFKPENLSKLKDCGVT
HHHHCCCCCCCCCCEEEECCCCCCCHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHCCEE
ILDTPSDMIPIALNYLGLDPHSTAPAEIEKAAALIKAIRPYVQNFHSSQYVTSLANGNTC
EECCCCCCEEHEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCEE
LAVGWSGDIIQARDRAVEAGNNIKVAYAIPKEGAPQWFDMLAIPKDAKHPDNAYAFINYL
EEECCCCCEEHHHHHHHHCCCCEEEEEECCCCCCCHHHHHEECCCCCCCCCHHHHHHHHH
LKSDVAAANTNFIHYANPVRTATPLVDAAIRNDPTIYPPPEVTAKMFTYAINPPDVDRLY
HHHCHHHCCCCEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCHHHHH
TRLWTEIKTGH
HHHHHHHCCCC
>Mature Secondary Structure 
TLRLLTLTLSITLLSGCGGAGAPGKTDAQAKVLNVYNYSDYIAEDTIPGFEKSTGIKVT
EEEEEEEEEEEHHHCCCCCCCCCCCCCCCEEEEEEECCHHHHHHCCCCCCCCCCCCEEE
YDVFDSDEMVETKLLAGGSDYDVVVPTLNFFGRQIQAGVFLPLDKSKIPNLVNLDPDVMR
EEECCCCHHHHHHHCCCCCCCEEEECCHHHCCHHHCCCEEEECCHHHCCCCCCCCHHHHH
RIAAQDPNNAYGVPYMIGTTGIGYNVDKLKAIFGNTDVANSWDLVFKPENLSKLKDCGVT
HHHHCCCCCCCCCCEEEECCCCCCCHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHCCEE
ILDTPSDMIPIALNYLGLDPHSTAPAEIEKAAALIKAIRPYVQNFHSSQYVTSLANGNTC
EECCCCCCEEHEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCEE
LAVGWSGDIIQARDRAVEAGNNIKVAYAIPKEGAPQWFDMLAIPKDAKHPDNAYAFINYL
EEECCCCCEEHHHHHHHHCCCCEEEEEECCCCCCCHHHHHEECCCCCCCCCHHHHHHHHH
LKSDVAAANTNFIHYANPVRTATPLVDAAIRNDPTIYPPPEVTAKMFTYAINPPDVDRLY
HHHCHHHCCCCEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCHHHHH
TRLWTEIKTGH
HHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: Polyamine Binding Protein [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503; 9298646; 9651355 [H]