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The map label for this gene is argD [C]

Identifier: 78048211

GI number: 78048211

Start: 2991952

End: 2993319

Strand: Reverse

Name: argD [C]

Synonym: XCV2655

Alternate gene names: 78048211

Gene position: 2993319-2991952 (Counterclockwise)

Preceding gene: 78048212

Following gene: 78048210

Centisome position: 57.8

GC content: 66.37

Gene sequence:

>1368_bases
GTGGATCCTTCGGTGCTCAAGGCGTTGCAGCGGCTCGACGCCGCCCATCATCTGCATCCGTTCAACGACAACGCGGCATT
GGCGCACAAGGGCACGCGCATCCTGACGCGCGGTGAGGGCGTGTACGTGTGGGATGCCGACGGCAATCAGTTTCTGGATG
CGTTCGGCGGACTGTGGTGCGTCAATGTCGGCTACGGGCGCGAGGAACTGGCGCAGGCAGCGGCGCGTCAGATGGAGCAG
CTGGCCTACTACAACAGCTTCTTCCAGTGCACCACCGAACCCACCATCCGGCTGGCGGCCAAGCTGGCGGCGTTGGCGCC
GGGCGATTTGAATCATGCGTTCTTCGCCAACTCCGGGTCCGAGGCCAACGACACCATCCTGCGCCTGGTGCGGCATTTCT
GGGCCGTGCAGGGGCAGCCGCAAAAACGCATCTTCATCGGCCGCCACGATGGCTATCACGGCACCACCATGGCCGGCGCC
AGCCTGGGCGGCATGAAGGGGATGCACCGGCAGGGCGGGTTGCCGATTGCCGATATCCATCACATCGCACCGCCGTATCA
CTTCGGCGATGGCGGGCAGATGGATCCGGAGGACTACGGGTTGCTGGCGGCACGTCGTCTGGAGGACAAGATTCTGGAAC
TCGGCCCCGAACATGTGGCTGCGTTCATCGGTGAGCCGATCATGGGCGCGATCGGCGTCTATATCCCACCGCGCAGTTAC
TGGCCGGAAATCGAGCGGATTTGTCGGCGCTACGACGTCCTGCTGGTCGCCGATGAAGTGATCTGCGGTTTCGGCCGCAC
CGGCGAGTGGTTCGGTGCGGAGTACTTCGGCTTCCAGCCCGACGTGATGACGCTGGCCAAAGGCATCACCTCCGGCTACA
TCCCGCTGGCGGCAGCCATGTTCAACGACCGCGTGGCCGGTGTACTCAAGACCCAAGGCGGCGAGCTGGCGCACGGTGGT
ACCTATGCCGGGCATCCGGTGTGCGCGGCGGTGGCGCTGGAAAACCTGCGCCTGCTGCAGGACGAGGGCATTGTCGAGAC
CGCGCGCACGCAGGTTGCGCCGTACCTGGCGCAGCGCTGGGCCGCCCTGGGCGAGCACCGGTTGGTGGGCGAGGCACGCA
TCGCCGGGCTGGTCGGGGCGCTGGAGCTGGTGCCGGACAAGCAGCGCCGTGGTGCGTATTTCCCCGGGCGCGGGCGGGTA
GGGGCGATGTGTCGCGATTTCGCGCTGCGCCGCGGGCTGATCCTGCGCGCGACCTACGATGCCATGCTGCTGTCGCCGCC
GTTGATCATTACCCGGGAGCAGGTCGACGAACTGTTCGACAAGGCCTGGGCCGCGCTGGACGACACCGCGCACGCCTTGG
GTCGATAG

Upstream 100 bases:

>100_bases
GCCGGGTGTGCAAGCGATTGGCCGGGCGTTCTGCAAAACCGTCCACGGTCCGGCAACAGGGCCTGGGTATGCTTGTAACC
CCTCACGCGCGGAGCAGGTC

Downstream 100 bases:

>100_bases
CTCCTGCACAAGTCGTCCCACCGCACGGCAGCGGGCAGTGACGAGGTCGGTGAGCACGCATGCGCGCAGCGGTGCCTTCG
TGGCGGCTGCCGCGCTGGTC

Product: putative aminotransferase

Products: N-Acetyl-L-glutamate 5-semialdehyde; L-Glutamate [C]

Alternate protein names: NA

Number of amino acids: Translated: 455; Mature: 455

Protein sequence:

>455_residues
MDPSVLKALQRLDAAHHLHPFNDNAALAHKGTRILTRGEGVYVWDADGNQFLDAFGGLWCVNVGYGREELAQAAARQMEQ
LAYYNSFFQCTTEPTIRLAAKLAALAPGDLNHAFFANSGSEANDTILRLVRHFWAVQGQPQKRIFIGRHDGYHGTTMAGA
SLGGMKGMHRQGGLPIADIHHIAPPYHFGDGGQMDPEDYGLLAARRLEDKILELGPEHVAAFIGEPIMGAIGVYIPPRSY
WPEIERICRRYDVLLVADEVICGFGRTGEWFGAEYFGFQPDVMTLAKGITSGYIPLAAAMFNDRVAGVLKTQGGELAHGG
TYAGHPVCAAVALENLRLLQDEGIVETARTQVAPYLAQRWAALGEHRLVGEARIAGLVGALELVPDKQRRGAYFPGRGRV
GAMCRDFALRRGLILRATYDAMLLSPPLIITREQVDELFDKAWAALDDTAHALGR

Sequences:

>Translated_455_residues
MDPSVLKALQRLDAAHHLHPFNDNAALAHKGTRILTRGEGVYVWDADGNQFLDAFGGLWCVNVGYGREELAQAAARQMEQ
LAYYNSFFQCTTEPTIRLAAKLAALAPGDLNHAFFANSGSEANDTILRLVRHFWAVQGQPQKRIFIGRHDGYHGTTMAGA
SLGGMKGMHRQGGLPIADIHHIAPPYHFGDGGQMDPEDYGLLAARRLEDKILELGPEHVAAFIGEPIMGAIGVYIPPRSY
WPEIERICRRYDVLLVADEVICGFGRTGEWFGAEYFGFQPDVMTLAKGITSGYIPLAAAMFNDRVAGVLKTQGGELAHGG
TYAGHPVCAAVALENLRLLQDEGIVETARTQVAPYLAQRWAALGEHRLVGEARIAGLVGALELVPDKQRRGAYFPGRGRV
GAMCRDFALRRGLILRATYDAMLLSPPLIITREQVDELFDKAWAALDDTAHALGR
>Mature_455_residues
MDPSVLKALQRLDAAHHLHPFNDNAALAHKGTRILTRGEGVYVWDADGNQFLDAFGGLWCVNVGYGREELAQAAARQMEQ
LAYYNSFFQCTTEPTIRLAAKLAALAPGDLNHAFFANSGSEANDTILRLVRHFWAVQGQPQKRIFIGRHDGYHGTTMAGA
SLGGMKGMHRQGGLPIADIHHIAPPYHFGDGGQMDPEDYGLLAARRLEDKILELGPEHVAAFIGEPIMGAIGVYIPPRSY
WPEIERICRRYDVLLVADEVICGFGRTGEWFGAEYFGFQPDVMTLAKGITSGYIPLAAAMFNDRVAGVLKTQGGELAHGG
TYAGHPVCAAVALENLRLLQDEGIVETARTQVAPYLAQRWAALGEHRLVGEARIAGLVGALELVPDKQRRGAYFPGRGRV
GAMCRDFALRRGLILRATYDAMLLSPPLIITREQVDELFDKAWAALDDTAHALGR

Specific function: Involved In Both The Arginine And Lysine Biosynthetic Pathways. [C]

COG id: COG0161

COG function: function code H; Adenosylmethionine-8-amino-7-oxononanoate aminotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI13994255, Length=440, Percent_Identity=27.9545454545455, Blast_Score=136, Evalue=5e-32,
Organism=Homo sapiens, GI37574042, Length=431, Percent_Identity=27.8422273781903, Blast_Score=135, Evalue=8e-32,
Organism=Homo sapiens, GI4557809, Length=431, Percent_Identity=26.6821345707657, Blast_Score=134, Evalue=3e-31,
Organism=Homo sapiens, GI226442705, Length=431, Percent_Identity=28.0742459396752, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI226442709, Length=403, Percent_Identity=27.7915632754342, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI24119277, Length=432, Percent_Identity=27.0833333333333, Blast_Score=119, Evalue=7e-27,
Organism=Homo sapiens, GI284507298, Length=324, Percent_Identity=28.0864197530864, Blast_Score=105, Evalue=9e-23,
Organism=Escherichia coli, GI1789759, Length=404, Percent_Identity=27.970297029703, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI145693181, Length=407, Percent_Identity=28.992628992629, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1786991, Length=450, Percent_Identity=28.2222222222222, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI1788044, Length=416, Percent_Identity=28.3653846153846, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1789016, Length=418, Percent_Identity=27.2727272727273, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI1787560, Length=314, Percent_Identity=30.2547770700637, Blast_Score=123, Evalue=3e-29,
Organism=Escherichia coli, GI1786349, Length=318, Percent_Identity=25.7861635220126, Blast_Score=84, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI32564660, Length=457, Percent_Identity=28.0087527352298, Blast_Score=144, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI25144271, Length=426, Percent_Identity=27.4647887323944, Blast_Score=139, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI71992977, Length=443, Percent_Identity=26.86230248307, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI25144274, Length=247, Percent_Identity=29.9595141700405, Blast_Score=113, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6323470, Length=454, Percent_Identity=26.431718061674, Blast_Score=145, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6324386, Length=491, Percent_Identity=25.6619144602851, Blast_Score=121, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6324432, Length=320, Percent_Identity=24.6875, Blast_Score=109, Evalue=7e-25,
Organism=Drosophila melanogaster, GI21356575, Length=417, Percent_Identity=30.6954436450839, Blast_Score=150, Evalue=1e-36,
Organism=Drosophila melanogaster, GI21357415, Length=431, Percent_Identity=27.3781902552204, Blast_Score=138, Evalue=1e-32,
Organism=Drosophila melanogaster, GI161085790, Length=449, Percent_Identity=28.9532293986637, Blast_Score=136, Evalue=3e-32,
Organism=Drosophila melanogaster, GI28574759, Length=462, Percent_Identity=28.1385281385281, Blast_Score=135, Evalue=6e-32,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: 2.6.1.11 [C]

Molecular weight: Translated: 49751; Mature: 49751

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDPSVLKALQRLDAAHHLHPFNDNAALAHKGTRILTRGEGVYVWDADGNQFLDAFGGLWC
CCHHHHHHHHHHHHHHCCCCCCCCCEEEECCCEEEEECCEEEEECCCCCHHHHHHCCEEE
VNVGYGREELAQAAARQMEQLAYYNSFFQCTTEPTIRLAAKLAALAPGDLNHAFFANSGS
EECCCCHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHCCCCCCCCEEEECCCC
EANDTILRLVRHFWAVQGQPQKRIFIGRHDGYHGTTMAGASLGGMKGMHRQGGLPIADIH
CHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCHHCCCCCHHHHH
HIAPPYHFGDGGQMDPEDYGLLAARRLEDKILELGPEHVAAFIGEPIMGAIGVYIPPRSY
HCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHCEEECCCCC
WPEIERICRRYDVLLVADEVICGFGRTGEWFGAEYFGFQPDVMTLAKGITSGYIPLAAAM
CHHHHHHHHHHCEEEEECHHHHCCCCCCCCCCCHHCCCCCHHHHHHHHHCCCCHHHHHHH
FNDRVAGVLKTQGGELAHGGTYAGHPVCAAVALENLRLLQDEGIVETARTQVAPYLAQRW
HHHHHHHHEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
AALGEHRLVGEARIAGLVGALELVPDKQRRGAYFPGRGRVGAMCRDFALRRGLILRATYD
HHHCCHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCHHHHHHHHHHHCCEEEEEEHH
AMLLSPPLIITREQVDELFDKAWAALDDTAHALGR
HHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDPSVLKALQRLDAAHHLHPFNDNAALAHKGTRILTRGEGVYVWDADGNQFLDAFGGLWC
CCHHHHHHHHHHHHHHCCCCCCCCCEEEECCCEEEEECCEEEEECCCCCHHHHHHCCEEE
VNVGYGREELAQAAARQMEQLAYYNSFFQCTTEPTIRLAAKLAALAPGDLNHAFFANSGS
EECCCCHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHCCCCCCCCEEEECCCC
EANDTILRLVRHFWAVQGQPQKRIFIGRHDGYHGTTMAGASLGGMKGMHRQGGLPIADIH
CHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCHHCCCCCHHHHH
HIAPPYHFGDGGQMDPEDYGLLAARRLEDKILELGPEHVAAFIGEPIMGAIGVYIPPRSY
HCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHCEEECCCCC
WPEIERICRRYDVLLVADEVICGFGRTGEWFGAEYFGFQPDVMTLAKGITSGYIPLAAAM
CHHHHHHHHHHCEEEEECHHHHCCCCCCCCCCCHHCCCCCHHHHHHHHHCCCCHHHHHHH
FNDRVAGVLKTQGGELAHGGTYAGHPVCAAVALENLRLLQDEGIVETARTQVAPYLAQRW
HHHHHHHHEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
AALGEHRLVGEARIAGLVGALELVPDKQRRGAYFPGRGRVGAMCRDFALRRGLILRATYD
HHHCCHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCHHHHHHHHHHHCCEEEEEEHH
AMLLSPPLIITREQVDELFDKAWAALDDTAHALGR
HHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Pyridoxal 5-phosphate [C]

Metal ions: Ni2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 3.1 {N2-acetyl-L-ornithine}} 1.54 {N2-acetyl-L-ornithine}} 1.3 {N2-acetyl-L-ornithine}} 1.1 {N2-acetyl-L-ornithine}} 0.48 {N2-acetyl-L-ornithine}} 0.34 {N2-acetyl-L-ornithine}} 2.5 {2-oxoglutarate}} [C]

Substrates: N2-Acetyl-L-ornithine; 2-Oxoglutarate [C]

Specific reaction: N2-Acetyl-L-ornithine + 2-Oxoglutarate <==> N-Acetyl-L-glutamate 5-semialdehyde + L-Glutamate [C]

General reaction: Amino group transfer [C]

Inhibitor: Cu2+; Ornithine; p-Chloromercuribenzoate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8796346; 9163424 [H]