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The map label for this gene is emrA [H]

Identifier: 78048209

GI number: 78048209

Start: 2989165

End: 2990367

Strand: Reverse

Name: emrA [H]

Synonym: XCV2653

Alternate gene names: 78048209

Gene position: 2990367-2989165 (Counterclockwise)

Preceding gene: 78048210

Following gene: 78048208

Centisome position: 57.75

GC content: 67.08

Gene sequence:

>1203_bases
TTGAGCAACCACGACCATCAGTCCAATGACGGCCAGTCCTCGGCCACGGATGCGCAGGGCGCCGGCGCGCGCGACGAGCA
GCAGGCGCCCAAGCCCTCGCCGTTGAAGAACCCCAAGGTGAAGTGGACGCTGATCCTGGTCGGGGTGCTGGTGCTGATCC
TGCTCGTGGTGTGGCTGGCCTATTACCTGATCAAGGGCCGCTACATGCAGGACACCAACAACGCCTATCTGCAGGCCGAT
TCGGTGGCGGTGGCACCGCGCGTCAGCGGCTACGTGACCAAGGTGCTGGTGAGCGACAACCAGATCGTGGAAGCCGGCCA
GCCGTTGCTGCAGATCGACGATCGCACCTACCAGGCCACCCTGCAGCAAGCCGAAGCCGCCATTGCCGCACGCCAGGCCG
ATATCGTCGCCGCCACCGCCAATGTCTCCGCGCAGGAATCGGCGCTGCTGCAGGCGCGCACCCAGGTCACCGCCGCTGCT
GCCAGCCTCAAGTTCGCCCAGGCCGAAGTGAAGCGCTTCGCGCCGTTGGCCGCCTCCGGCGCAGACACCCACGAACATCA
GGAAAGCCTGCAGCACGATCTGGCACGCGCACGTGCGCAGTACGACGCCGCGCAGGCGCAGGCCAAGGCCGGCGAGAGCC
AGATCCAGGCCAGCCGCGCGCAACTGGAACAGGCACAGGCCGGCGTGAAACAGGCCACCGCCGATGCCGACCAGGCGCGG
GTGGCGGTGGAAGACACCCGTCTGACCAGCCGTATCCACGGCCGGGTGGGCGACAAGACCGTGCAGGTGGGCCAGTTCCT
CGGCGCCGGCACCCGCACCATGACCATCGTGCCGCAGCAATCGCTGTATCTGGTTGCCAATTTCAAGGAAACCCAGGTCG
GCCTGATGCGTCCCGGCCAGCCGGCCGAGATCGAGGTCGATGCGCTGTCCGGAGTGAAGTTGCACGGCAAGGTCGAGAGC
CTGTCGCCGGGCACCGGCTCGCAGTTCGCCTTGCTACCGCCGGAAAACGCCACCGGCAATTTCACCAAGGTGGTACAGCG
TGTGCCGGTGCGCATCCGTGTGCTGGCCGGTGAAGAGGCGCGCAAGGTGCTGGTGCCGGGCATGTCGGTGGAGGTCACCG
TGGACACGCGCTCGGCCAAGGACGCCAAGCAGCGTGCCGAAGAAGAATCCGACCGCGTGCAGGAGCAGGAGAGCGCGCGA
TGA

Upstream 100 bases:

>100_bases
CCGGGCAGTTCAAATAATTGTATGGTTGCGCCGCCATTGCCGACGCCGCCGAGACGGCCGCTGCGTGGCGCGCCTCGCCC
ATCACACAGGACGCATCCTT

Downstream 100 bases:

>100_bases
GTGCAGCGGCCGCCACCGGCCCGCGTGCCGGCGGCAGCGCCCAGCGCGAGAAGGCCGAGCCGGGCGCCTGGCTGGCGGTG
CTGGCCGGCACCATCGGCTC

Product: membrane fusion protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MSNHDHQSNDGQSSATDAQGAGARDEQQAPKPSPLKNPKVKWTLILVGVLVLILLVVWLAYYLIKGRYMQDTNNAYLQAD
SVAVAPRVSGYVTKVLVSDNQIVEAGQPLLQIDDRTYQATLQQAEAAIAARQADIVAATANVSAQESALLQARTQVTAAA
ASLKFAQAEVKRFAPLAASGADTHEHQESLQHDLARARAQYDAAQAQAKAGESQIQASRAQLEQAQAGVKQATADADQAR
VAVEDTRLTSRIHGRVGDKTVQVGQFLGAGTRTMTIVPQQSLYLVANFKETQVGLMRPGQPAEIEVDALSGVKLHGKVES
LSPGTGSQFALLPPENATGNFTKVVQRVPVRIRVLAGEEARKVLVPGMSVEVTVDTRSAKDAKQRAEEESDRVQEQESAR

Sequences:

>Translated_400_residues
MSNHDHQSNDGQSSATDAQGAGARDEQQAPKPSPLKNPKVKWTLILVGVLVLILLVVWLAYYLIKGRYMQDTNNAYLQAD
SVAVAPRVSGYVTKVLVSDNQIVEAGQPLLQIDDRTYQATLQQAEAAIAARQADIVAATANVSAQESALLQARTQVTAAA
ASLKFAQAEVKRFAPLAASGADTHEHQESLQHDLARARAQYDAAQAQAKAGESQIQASRAQLEQAQAGVKQATADADQAR
VAVEDTRLTSRIHGRVGDKTVQVGQFLGAGTRTMTIVPQQSLYLVANFKETQVGLMRPGQPAEIEVDALSGVKLHGKVES
LSPGTGSQFALLPPENATGNFTKVVQRVPVRIRVLAGEEARKVLVPGMSVEVTVDTRSAKDAKQRAEEESDRVQEQESAR
>Mature_399_residues
SNHDHQSNDGQSSATDAQGAGARDEQQAPKPSPLKNPKVKWTLILVGVLVLILLVVWLAYYLIKGRYMQDTNNAYLQADS
VAVAPRVSGYVTKVLVSDNQIVEAGQPLLQIDDRTYQATLQQAEAAIAARQADIVAATANVSAQESALLQARTQVTAAAA
SLKFAQAEVKRFAPLAASGADTHEHQESLQHDLARARAQYDAAQAQAKAGESQIQASRAQLEQAQAGVKQATADADQARV
AVEDTRLTSRIHGRVGDKTVQVGQFLGAGTRTMTIVPQQSLYLVANFKETQVGLMRPGQPAEIEVDALSGVKLHGKVESL
SPGTGSQFALLPPENATGNFTKVVQRVPVRIRVLAGEEARKVLVPGMSVEVTVDTRSAKDAKQRAEEESDRVQEQESAR

Specific function: The emr locus confers resistance to substances of high hydrophobicity. EmrA probably participate in a transport system to extrude toxins and drugs from the cell [H]

COG id: COG1566

COG function: function code V; Multidrug resistance efflux pump

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789041, Length=357, Percent_Identity=34.453781512605, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI1788711, Length=338, Percent_Identity=30.7692307692308, Blast_Score=138, Evalue=6e-34,
Organism=Escherichia coli, GI1790519, Length=291, Percent_Identity=34.020618556701, Blast_Score=128, Evalue=5e-31,
Organism=Escherichia coli, GI87081951, Length=331, Percent_Identity=25.3776435045317, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1789637, Length=351, Percent_Identity=27.6353276353276, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1790024, Length=300, Percent_Identity=26.6666666666667, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1789900, Length=310, Percent_Identity=25.1612903225806, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1790012, Length=300, Percent_Identity=26.6666666666667, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005694
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 42896; Mature: 42764

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNHDHQSNDGQSSATDAQGAGARDEQQAPKPSPLKNPKVKWTLILVGVLVLILLVVWLA
CCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHH
YYLIKGRYMQDTNNAYLQADSVAVAPRVSGYVTKVLVSDNQIVEAGQPLLQIDDRTYQAT
HHHHCCCEEECCCCCEEEECCEEECCCCCCCEEEEEECCCCEECCCCCEEEECCHHHHHH
LQQAEAAIAARQADIVAATANVSAQESALLQARTQVTAAAASLKFAQAEVKRFAPLAASG
HHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
ADTHEHQESLQHDLARARAQYDAAQAQAKAGESQIQASRAQLEQAQAGVKQATADADQAR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHE
VAVEDTRLTSRIHGRVGDKTVQVGQFLGAGTRTMTIVPQQSLYLVANFKETQVGLMRPGQ
EEEHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEEECCCCEEEEEECCCCEEECCCCCC
PAEIEVDALSGVKLHGKVESLSPGTGSQFALLPPENATGNFTKVVQRVPVRIRVLAGEEA
CCEEEEECCCCEEEECEECCCCCCCCCEEEEECCCCCCCHHHHHHHHCCEEEEEEECCCC
RKVLVPGMSVEVTVDTRSAKDAKQRAEEESDRVQEQESAR
CEEEECCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SNHDHQSNDGQSSATDAQGAGARDEQQAPKPSPLKNPKVKWTLILVGVLVLILLVVWLA
CCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHH
YYLIKGRYMQDTNNAYLQADSVAVAPRVSGYVTKVLVSDNQIVEAGQPLLQIDDRTYQAT
HHHHCCCEEECCCCCEEEECCEEECCCCCCCEEEEEECCCCEECCCCCEEEECCHHHHHH
LQQAEAAIAARQADIVAATANVSAQESALLQARTQVTAAAASLKFAQAEVKRFAPLAASG
HHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
ADTHEHQESLQHDLARARAQYDAAQAQAKAGESQIQASRAQLEQAQAGVKQATADADQAR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHE
VAVEDTRLTSRIHGRVGDKTVQVGQFLGAGTRTMTIVPQQSLYLVANFKETQVGLMRPGQ
EEEHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEEECCCCEEEEEECCCCEEECCCCCC
PAEIEVDALSGVKLHGKVESLSPGTGSQFALLPPENATGNFTKVVQRVPVRIRVLAGEEA
CCEEEEECCCCEEEECEECCCCCCCCCEEEEECCCCCCCHHHHHHHHCCEEEEEEECCCC
RKVLVPGMSVEVTVDTRSAKDAKQRAEEESDRVQEQESAR
CEEEECCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1409590; 9205837; 9278503; 8203018 [H]