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The map label for this gene is smpB [H]

Identifier: 78047112

GI number: 78047112

Start: 1756183

End: 1756686

Strand: Reverse

Name: smpB [H]

Synonym: XCV1556

Alternate gene names: 78047112

Gene position: 1756686-1756183 (Counterclockwise)

Preceding gene: 78047115

Following gene: 78047109

Centisome position: 33.92

GC content: 63.49

Gene sequence:

>504_bases
ATGAGCAAGAAACAGACCAAGGATAAAGCAAACGGCGCGAAGGCCACGAAAACCATCGCGTTGAACAAGCGTGCGCGCCA
CGAATACCACCTGGAAGAACGCTACGAAGCCGGCCTGGCACTGCAGGGCTGGGAGGTCAAGGCGATCCGCGCCGGCCGCG
CCAACATCGTCGACGGCTATGCCTATGTGCGCTCCGGCGAGATCTACCTGATCGGCGCACAGATCACGCCGCTGATCCAG
GCCTCCACCCACACGGTGCCAGTGGAACGGCGCGACCGTAAACTGCTGCTGCACCGGGCCGAGATCGACAAGGTGCTCAC
CCGCGTGGAGCGCGAAGGCTACACCCTGGTGCCTACCGCGCTGTACTGGAGCAGCAACAAGGTCAAGCTGGAAATCGCCC
TGGCCAAGGGCAAGCAGAATCACGACAAGCGCGACGCCGCCAAGGACCGCGACTGGCAGCGCGACAAGCAGCGCGTGATG
CGGCGGCATAACCGCGATGCGTGA

Upstream 100 bases:

>100_bases
CGCTGTGTTCGACCAAGGCGCTGCGACGGATGGTAGGCATTCGCTAACTTGTGAAAGACGGAAGATCGTTTGACGACACC
GAATCGGCGACAATGGGAGG

Downstream 100 bases:

>100_bases
TGAGCGGGGATTGGTGATTCGGGATTAGGGATTGGTTAGAGCGGCTGTGCGTGGCCTTTTGCACTGCTGTCCTGCGATCC
ACACCTGCAGAGCGGCGAGG

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 167; Mature: 166

Protein sequence:

>167_residues
MSKKQTKDKANGAKATKTIALNKRARHEYHLEERYEAGLALQGWEVKAIRAGRANIVDGYAYVRSGEIYLIGAQITPLIQ
ASTHTVPVERRDRKLLLHRAEIDKVLTRVEREGYTLVPTALYWSSNKVKLEIALAKGKQNHDKRDAAKDRDWQRDKQRVM
RRHNRDA

Sequences:

>Translated_167_residues
MSKKQTKDKANGAKATKTIALNKRARHEYHLEERYEAGLALQGWEVKAIRAGRANIVDGYAYVRSGEIYLIGAQITPLIQ
ASTHTVPVERRDRKLLLHRAEIDKVLTRVEREGYTLVPTALYWSSNKVKLEIALAKGKQNHDKRDAAKDRDWQRDKQRVM
RRHNRDA
>Mature_166_residues
SKKQTKDKANGAKATKTIALNKRARHEYHLEERYEAGLALQGWEVKAIRAGRANIVDGYAYVRSGEIYLIGAQITPLIQA
STHTVPVERRDRKLLLHRAEIDKVLTRVEREGYTLVPTALYWSSNKVKLEIALAKGKQNHDKRDAAKDRDWQRDKQRVMR
RHNRDA

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes [H]

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family [H]

Homologues:

Organism=Escherichia coli, GI1788973, Length=160, Percent_Identity=56.25, Blast_Score=182, Evalue=1e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000037
- InterPro:   IPR020081
- ProDom:   PD004488 [H]

Pfam domain/function: PF01668 SmpB [H]

EC number: NA

Molecular weight: Translated: 19269; Mature: 19138

Theoretical pI: Translated: 10.75; Mature: 10.75

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKQTKDKANGAKATKTIALNKRARHEYHLEERYEAGLALQGWEVKAIRAGRANIVDGY
CCCCCCCCCCCCCCHHEEEEECCHHHHHHHHHHHHCCCCEECCCEEEEEECCCCCEEECE
AYVRSGEIYLIGAQITPLIQASTHTVPVERRDRKLLLHRAEIDKVLTRVEREGYTLVPTA
EEEECCCEEEECCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEE
LYWSSNKVKLEIALAKGKQNHDKRDAAKDRDWQRDKQRVMRRHNRDA
EEECCCEEEEEEEEECCCCCCCHHHHHCCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SKKQTKDKANGAKATKTIALNKRARHEYHLEERYEAGLALQGWEVKAIRAGRANIVDGY
CCCCCCCCCCCCCHHEEEEECCHHHHHHHHHHHHCCCCEECCCEEEEEECCCCCEEECE
AYVRSGEIYLIGAQITPLIQASTHTVPVERRDRKLLLHRAEIDKVLTRVEREGYTLVPTA
EEEECCCEEEECCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEE
LYWSSNKVKLEIALAKGKQNHDKRDAAKDRDWQRDKQRVMRRHNRDA
EEECCCEEEEEEEEECCCCCCCHHHHHCCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]