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The map label for this gene is moeB [H]

Identifier: 78043264

GI number: 78043264

Start: 1126293

End: 1126898

Strand: Direct

Name: moeB [H]

Synonym: CHY_1264

Alternate gene names: 78043264

Gene position: 1126293-1126898 (Clockwise)

Preceding gene: 78045152

Following gene: 78044019

Centisome position: 46.9

GC content: 37.13

Gene sequence:

>606_bases
ATGAGCTCCTTTAATTTAATGCTAAAAAATTATTTTAATGATGATTTGTTGCAGAAACTAAGCAGAGTAAAAGTACTAAT
TATCGGCTGTGGAGGGCTTGGGTCACACATTGCCTGGATGCTAATTCGTTGCGGGTTCCAGAACTTGAAGATTGTTGATT
TTGATCGGGTGGAGCTTAAGAATTTAAATAGGCAAGGTTATTTTTTTACGCAGGTGGGTCTACCTAAGGTATTAGCCTTA
AAAGATATTCTTACCCAAATTAATCCAGGGGCTAATATTGCTATAGCAGTTGAAAAAATTGATGGTAAAAATGTTGAAAA
AATAATTATGTCCTATGATGTTATTGTGGAGGCTGTAGATGATGAAACAACAAAAGCTTTAATTTTCGAAGCAGCTATGA
AACTAAACAAAAAGGTTGTTGCAGCTTCCGGAGTAGCTGGCTTTGGCGATTGTGAGCGTATAAAAATCAAAAGAGGTAGG
AACTTTGTTATTGTTGGTGATTTTGCAACTTCTGTCAAAGATAAAAAACCTTATGCTCCTAAAGTAACGGCCGTTGCTGC
AATTCAGGCCGATGAGGTGTTAAGGATGGTGGTGGAGGATGAGTAA

Upstream 100 bases:

>100_bases
GAAGTTGAAGATCGAAGAATGGTAGCTGAGATAATTGAGGTTATAAGAAAAAAAGGGTTGAGGCCAGAGTTTACAAATTG
GATAAGAGGTGTTAGACCTT

Downstream 100 bases:

>100_bases
GCTGGAAAAGTTAAGACTTTTGGAAGATTATAATATTTACTGCATAACTGCTGAGAAGTTTTCTCGTGGTAGAGAAAATC
TTAAAGTTGTTTCTGAGATG

Product: thiamine biosynthesis protein ThiF

Products: ThiS-COSH; AMP; ThiS-COAMP; pyrophosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 201; Mature: 200

Protein sequence:

>201_residues
MSSFNLMLKNYFNDDLLQKLSRVKVLIIGCGGLGSHIAWMLIRCGFQNLKIVDFDRVELKNLNRQGYFFTQVGLPKVLAL
KDILTQINPGANIAIAVEKIDGKNVEKIIMSYDVIVEAVDDETTKALIFEAAMKLNKKVVAASGVAGFGDCERIKIKRGR
NFVIVGDFATSVKDKKPYAPKVTAVAAIQADEVLRMVVEDE

Sequences:

>Translated_201_residues
MSSFNLMLKNYFNDDLLQKLSRVKVLIIGCGGLGSHIAWMLIRCGFQNLKIVDFDRVELKNLNRQGYFFTQVGLPKVLAL
KDILTQINPGANIAIAVEKIDGKNVEKIIMSYDVIVEAVDDETTKALIFEAAMKLNKKVVAASGVAGFGDCERIKIKRGR
NFVIVGDFATSVKDKKPYAPKVTAVAAIQADEVLRMVVEDE
>Mature_200_residues
SSFNLMLKNYFNDDLLQKLSRVKVLIIGCGGLGSHIAWMLIRCGFQNLKIVDFDRVELKNLNRQGYFFTQVGLPKVLALK
DILTQINPGANIAIAVEKIDGKNVEKIIMSYDVIVEAVDDETTKALIFEAAMKLNKKVVAASGVAGFGDCERIKIKRGRN
FVIVGDFATSVKDKKPYAPKVTAVAAIQADEVLRMVVEDE

Specific function: Catalyzes the adenylation by ATP of the carboxyl group of the C-terminal glycine of sulfur carrier protein ThiS [H]

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesA/moeB/thiF family [H]

Homologues:

Organism=Escherichia coli, GI87082356, Length=133, Percent_Identity=36.0902255639098, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1787048, Length=199, Percent_Identity=28.1407035175879, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI1789177, Length=173, Percent_Identity=27.7456647398844, Blast_Score=62, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17540406, Length=129, Percent_Identity=32.5581395348837, Blast_Score=73, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6321903, Length=177, Percent_Identity=29.9435028248588, Blast_Score=73, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6321790, Length=157, Percent_Identity=28.0254777070064, Blast_Score=65, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24582879, Length=128, Percent_Identity=32.8125, Blast_Score=72, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012731
- InterPro:   IPR007901
- InterPro:   IPR009036
- InterPro:   IPR016040
- InterPro:   IPR000594 [H]

Pfam domain/function: PF05237 MoeZ_MoeB; PF00899 ThiF [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 22239; Mature: 22108

Theoretical pI: Translated: 9.13; Mature: 9.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSFNLMLKNYFNDDLLQKLSRVKVLIIGCGGLGSHIAWMLIRCGFQNLKIVDFDRVELK
CCCHHHHHHHHCCHHHHHHHHCEEEEEEECCCHHHHHHHHHHHCCCCCCEEEECCHHHHH
NLNRQGYFFTQVGLPKVLALKDILTQINPGANIAIAVEKIDGKNVEKIIMSYDVIVEAVD
CCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHEEEECC
DETTKALIFEAAMKLNKKVVAASGVAGFGDCERIKIKRGRNFVIVGDFATSVKDKKPYAP
CCHHHHHHHHHHHHHCCEEEEECCCCCCCCCCEEEEECCCEEEEEECCHHCCCCCCCCCC
KVTAVAAIQADEVLRMVVEDE
CEEEEEEHHHHHHHHHHHCCC
>Mature Secondary Structure 
SSFNLMLKNYFNDDLLQKLSRVKVLIIGCGGLGSHIAWMLIRCGFQNLKIVDFDRVELK
CCHHHHHHHHCCHHHHHHHHCEEEEEEECCCHHHHHHHHHHHCCCCCCEEEECCHHHHH
NLNRQGYFFTQVGLPKVLALKDILTQINPGANIAIAVEKIDGKNVEKIIMSYDVIVEAVD
CCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHEEEECC
DETTKALIFEAAMKLNKKVVAASGVAGFGDCERIKIKRGRNFVIVGDFATSVKDKKPYAP
CCHHHHHHHHHHHHHCCEEEEECCCCCCCCCCEEEEECCCEEEEEECCHHCCCCCCCCCC
KVTAVAAIQADEVLRMVVEDE
CEEEEEEHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ThiS-COAMP; L-cysteine; ThiS protein; ATP [C]

Specific reaction: ThiS-COAMP + L-cysteine = ThiS-COSH + AMP ThiS protein + ATP = ThiS-COAMP + pyrophosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8432721; 8265357; 9278503; 9632726; 10082377 [H]