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The map label for this gene is thiH [H]

Identifier: 78045152

GI number: 78045152

Start: 1125185

End: 1126306

Strand: Direct

Name: thiH [H]

Synonym: CHY_1263

Alternate gene names: 78045152

Gene position: 1125185-1126306 (Clockwise)

Preceding gene: 78043230

Following gene: 78043264

Centisome position: 46.85

GC content: 35.83

Gene sequence:

>1122_bases
GTGCTTGATTATTTACAAAAATGTTTTGAACTTTATTTTAGGTATGAGCAATATGTTCCTTCCTTTTCTGAAGCTTTGGA
TATTTTAGCAAAAGACTATTTGGATAAAATTGACTTAGTCAAGCTTTTAAATGTTGAGGATGAAGAAATAATAAAGTTTA
TGGCAAAAAAAGCCAAAAGAATTACGGAACTTAATTTTGGAAAAGTTATTTTATTGTATGCTCCATTGTATATAGCTAAC
TTTTGTGAAAATGGGTGTGTTTACTGTGGTTTTTCCAAACTAAGAAAATACCCACGGGAAAAACTTAGTTTAGAGCAAAT
GGAAGAAGAAATGCAGCAGATAAAAAGTGAGGGAATTGACTCTATTTTACTTTTAACCGGTGAGGACAGGAAAAATTCGC
CTTTTGCCTATATAAAAAATGCCTGTAAGCTTGCCACTAAATATTTTTCAGAGGTTTCAATCGAAGTTTATCCCCTTTCC
AAAGAAGAGTACGAAGAATTAGCAAGAATTGGGGTTATAGGTACTACCATTTATCAAGAAACGTATATAAAGAAAGATTA
TGAAAAGCTGCATCTTTTTGGTCCAAAAAAAGATTATGAATTTAGGCTTTATACTCCGGAAAGAGCATTAAAAGCGGGGT
TTAAAGCGGCAAGTATAGGACCGCTTTTAGGATTATCTTTGCCCAAACTGGATGTGTATTCGGCAATTTTACATGCTGAC
TATTTAATGAAAAAGTATCCTCAAGCGGAGATTGCGATTTCTTTTCCAAGACTAAGGGCTGCCAATACGGGATTTAAGGC
CAAACATGTGGTATCCGATAAAGAGTTTATTAAGTTTTTGCTTGTGACCAGAATCTATTTGCCCCGGATTGGTATTAATC
TTTCGACGCGGGAGAGGCCAAGCTTACGAGATGCTCTTCTGGACATTTGCATTACAAAGATGTCAGCCGGTTCCAAAACA
ACTGTCGGAGGGTATTTTAGCAAAAAGGAGGATTCCCAAGGGCAGTTTGAAGTTGAAGATCGAAGAATGGTAGCTGAGAT
AATTGAGGTTATAAGAAAAAAAGGGTTGAGGCCAGAGTTTACAAATTGGATAAGAGGTGTTAGACCTTATGAGCTCCTTT
AA

Upstream 100 bases:

>100_bases
GACTATTAAAAGAGTATGAATATGCTCAGGCATCGTCTCCTCTTGAAGATTTTATAAAGGGATAAAGAGTTTAATTTAAA
AAGGTTAGAAGGTGTTAGCT

Downstream 100 bases:

>100_bases
TTTAATGCTAAAAAATTATTTTAATGATGATTTGTTGCAGAAACTAAGCAGAGTAAAAGTACTAATTATCGGCTGTGGAG
GGCTTGGGTCACACATTGCC

Product: thiamine biosynthesis protein ThiH

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: Dehydroglycine synthase; Tyrosine lyase [H]

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MLDYLQKCFELYFRYEQYVPSFSEALDILAKDYLDKIDLVKLLNVEDEEIIKFMAKKAKRITELNFGKVILLYAPLYIAN
FCENGCVYCGFSKLRKYPREKLSLEQMEEEMQQIKSEGIDSILLLTGEDRKNSPFAYIKNACKLATKYFSEVSIEVYPLS
KEEYEELARIGVIGTTIYQETYIKKDYEKLHLFGPKKDYEFRLYTPERALKAGFKAASIGPLLGLSLPKLDVYSAILHAD
YLMKKYPQAEIAISFPRLRAANTGFKAKHVVSDKEFIKFLLVTRIYLPRIGINLSTRERPSLRDALLDICITKMSAGSKT
TVGGYFSKKEDSQGQFEVEDRRMVAEIIEVIRKKGLRPEFTNWIRGVRPYELL

Sequences:

>Translated_373_residues
MLDYLQKCFELYFRYEQYVPSFSEALDILAKDYLDKIDLVKLLNVEDEEIIKFMAKKAKRITELNFGKVILLYAPLYIAN
FCENGCVYCGFSKLRKYPREKLSLEQMEEEMQQIKSEGIDSILLLTGEDRKNSPFAYIKNACKLATKYFSEVSIEVYPLS
KEEYEELARIGVIGTTIYQETYIKKDYEKLHLFGPKKDYEFRLYTPERALKAGFKAASIGPLLGLSLPKLDVYSAILHAD
YLMKKYPQAEIAISFPRLRAANTGFKAKHVVSDKEFIKFLLVTRIYLPRIGINLSTRERPSLRDALLDICITKMSAGSKT
TVGGYFSKKEDSQGQFEVEDRRMVAEIIEVIRKKGLRPEFTNWIRGVRPYELL
>Mature_373_residues
MLDYLQKCFELYFRYEQYVPSFSEALDILAKDYLDKIDLVKLLNVEDEEIIKFMAKKAKRITELNFGKVILLYAPLYIAN
FCENGCVYCGFSKLRKYPREKLSLEQMEEEMQQIKSEGIDSILLLTGEDRKNSPFAYIKNACKLATKYFSEVSIEVYPLS
KEEYEELARIGVIGTTIYQETYIKKDYEKLHLFGPKKDYEFRLYTPERALKAGFKAASIGPLLGLSLPKLDVYSAILHAD
YLMKKYPQAEIAISFPRLRAANTGFKAKHVVSDKEFIKFLLVTRIYLPRIGINLSTRERPSLRDALLDICITKMSAGSKT
TVGGYFSKKEDSQGQFEVEDRRMVAEIIEVIRKKGLRPEFTNWIRGVRPYELL

Specific function: Catalyzes the radical-mediated cleavage of tyrosine to 2-iminoacetate and 4-cresol [H]

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. ThiH family [H]

Homologues:

Organism=Escherichia coli, GI1790423, Length=336, Percent_Identity=33.0357142857143, Blast_Score=216, Evalue=2e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR007197
- InterPro:   IPR012726 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 43222; Mature: 43222

Theoretical pI: Translated: 8.92; Mature: 8.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDYLQKCFELYFRYEQYVPSFSEALDILAKDYLDKIDLVKLLNVEDEEIIKFMAKKAKR
CHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
ITELNFGKVILLYAPLYIANFCENGCVYCGFSKLRKYPREKLSLEQMEEEMQQIKSEGID
HHHCCCCCCHHHHHHHHHHHHHCCCCEEECHHHHHHCCHHHCCHHHHHHHHHHHHHCCCC
SILLLTGEDRKNSPFAYIKNACKLATKYFSEVSIEVYPLSKEEYEELARIGVIGTTIYQE
EEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCEEEEEECCHHHHHHHHHHHHHHHHHHHH
TYIKKDYEKLHLFGPKKDYEFRLYTPERALKAGFKAASIGPLLGLSLPKLDVYSAILHAD
HHHHHHHHHHEECCCCCCCCEEEECHHHHHHCCCCHHCCCHHHCCCCCHHHHHHHHHHHH
YLMKKYPQAEIAISFPRLRAANTGFKAKHVVSDKEFIKFLLVTRIYLPRIGINLSTRERP
HHHHHCCCCCEEEECCHHHCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCC
SLRDALLDICITKMSAGSKTTVGGYFSKKEDSQGQFEVEDRRMVAEIIEVIRKKGLRPEF
HHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHCCCCCCH
TNWIRGVRPYELL
HHHHCCCCCCCCC
>Mature Secondary Structure
MLDYLQKCFELYFRYEQYVPSFSEALDILAKDYLDKIDLVKLLNVEDEEIIKFMAKKAKR
CHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
ITELNFGKVILLYAPLYIANFCENGCVYCGFSKLRKYPREKLSLEQMEEEMQQIKSEGID
HHHCCCCCCHHHHHHHHHHHHHCCCCEEECHHHHHHCCHHHCCHHHHHHHHHHHHHCCCC
SILLLTGEDRKNSPFAYIKNACKLATKYFSEVSIEVYPLSKEEYEELARIGVIGTTIYQE
EEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCEEEEEECCHHHHHHHHHHHHHHHHHHHH
TYIKKDYEKLHLFGPKKDYEFRLYTPERALKAGFKAASIGPLLGLSLPKLDVYSAILHAD
HHHHHHHHHHEECCCCCCCCEEEECHHHHHHCCCCHHCCCHHHCCCCCHHHHHHHHHHHH
YLMKKYPQAEIAISFPRLRAANTGFKAKHVVSDKEFIKFLLVTRIYLPRIGINLSTRERP
HHHHHCCCCCEEEECCHHHCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCC
SLRDALLDICITKMSAGSKTTVGGYFSKKEDSQGQFEVEDRRMVAEIIEVIRKKGLRPEF
HHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHCCCCCCH
TNWIRGVRPYELL
HHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609 [H]