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The map label for this gene is thiE [H]

Identifier: 78044019

GI number: 78044019

Start: 1126918

End: 1127565

Strand: Direct

Name: thiE [H]

Synonym: CHY_1265

Alternate gene names: 78044019

Gene position: 1126918-1127565 (Clockwise)

Preceding gene: 78043264

Following gene: 78044138

Centisome position: 46.93

GC content: 37.5

Gene sequence:

>648_bases
TTGGAAGATTATAATATTTACTGCATAACTGCTGAGAAGTTTTCTCGTGGTAGAGAAAATCTTAAAGTTGTTTCTGAGAT
GCTAAAAGCTGGAATAAGGATAATTCAGTATCGAGAGAAATATAAGTCACTGAAGGAAAAATATTATGAATGCCTCAAAA
TAAGAGAACTGACAAGACAATATGGGGCTATTTTGATTGTAAATGACCATGTGGATTTGTGTCAAATGGTTGACGCGGAT
GGGGTTCATCTTGGCCAAGACGATTATCCGGTTAAAGAGGCTCGGAGGATTTTAGGGGATGAATATATAATTGGAGTTTC
TACTCATTCTCCCGAACAGCTGAAAAAAGCAGCAAAGGAAGGAGCTGATTATGCAGGTGTTGGACCGCTTTTTGCCACGA
ATACTAAAGATAATGCAATACCCCCGGTTGGACTGGAATATCTAAAATGGGCAGTTGCAAACTCAAGTATACCCTTTGTT
GCTATAGGTGGGATAAAAGAATATAACATTCAGGAGGTTCTCGACTTGGGATCCAAGTGTATAGCTCTTGTTACAGAGAT
TGTTGGGGCTGAGGATATTAAAGATAAAATTGATAGACTAAATAAGATTTTAGAAAGATATAGGGTTAGTAACAAAGCCT
TCGATTAA

Upstream 100 bases:

>100_bases
AAACCTTATGCTCCTAAAGTAACGGCCGTTGCTGCAATTCAGGCCGATGAGGTGTTAAGGATGGTGGTGGAGGATGAGTA
AGCTGGAAAAGTTAAGACTT

Downstream 100 bases:

>100_bases
AGAGACGGGTATTAGTCCTAACGTTAATTGAAAAATACCGATATCTCTCCTTCTAATAATTTTCATAAGATAAATTAATT
GGACAAGAAAATTTTTCTAT

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 215; Mature: 215

Protein sequence:

>215_residues
MEDYNIYCITAEKFSRGRENLKVVSEMLKAGIRIIQYREKYKSLKEKYYECLKIRELTRQYGAILIVNDHVDLCQMVDAD
GVHLGQDDYPVKEARRILGDEYIIGVSTHSPEQLKKAAKEGADYAGVGPLFATNTKDNAIPPVGLEYLKWAVANSSIPFV
AIGGIKEYNIQEVLDLGSKCIALVTEIVGAEDIKDKIDRLNKILERYRVSNKAFD

Sequences:

>Translated_215_residues
MEDYNIYCITAEKFSRGRENLKVVSEMLKAGIRIIQYREKYKSLKEKYYECLKIRELTRQYGAILIVNDHVDLCQMVDAD
GVHLGQDDYPVKEARRILGDEYIIGVSTHSPEQLKKAAKEGADYAGVGPLFATNTKDNAIPPVGLEYLKWAVANSSIPFV
AIGGIKEYNIQEVLDLGSKCIALVTEIVGAEDIKDKIDRLNKILERYRVSNKAFD
>Mature_215_residues
MEDYNIYCITAEKFSRGRENLKVVSEMLKAGIRIIQYREKYKSLKEKYYECLKIRELTRQYGAILIVNDHVDLCQMVDAD
GVHLGQDDYPVKEARRILGDEYIIGVSTHSPEQLKKAAKEGADYAGVGPLFATNTKDNAIPPVGLEYLKWAVANSSIPFV
AIGGIKEYNIQEVLDLGSKCIALVTEIVGAEDIKDKIDRLNKILERYRVSNKAFD

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=186, Percent_Identity=32.258064516129, Blast_Score=82, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6325042, Length=232, Percent_Identity=34.051724137931, Blast_Score=116, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 24296; Mature: 24296

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEDYNIYCITAEKFSRGRENLKVVSEMLKAGIRIIQYREKYKSLKEKYYECLKIRELTRQ
CCCCEEEEEEHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YGAILIVNDHVDLCQMVDADGVHLGQDDYPVKEARRILGDEYIIGVSTHSPEQLKKAAKE
HCEEEEEECCHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCEEEEECCCCHHHHHHHHHC
GADYAGVGPLFATNTKDNAIPPVGLEYLKWAVANSSIPFVAIGGIKEYNIQEVLDLGSKC
CCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHCCHHH
IALVTEIVGAEDIKDKIDRLNKILERYRVSNKAFD
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MEDYNIYCITAEKFSRGRENLKVVSEMLKAGIRIIQYREKYKSLKEKYYECLKIRELTRQ
CCCCEEEEEEHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YGAILIVNDHVDLCQMVDADGVHLGQDDYPVKEARRILGDEYIIGVSTHSPEQLKKAAKE
HCEEEEEECCHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCEEEEECCCCHHHHHHHHHC
GADYAGVGPLFATNTKDNAIPPVGLEYLKWAVANSSIPFVAIGGIKEYNIQEVLDLGSKC
CCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHCCHHH
IALVTEIVGAEDIKDKIDRLNKILERYRVSNKAFD
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA