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The map label for this gene is purH [H]

Identifier: 78043098

GI number: 78043098

Start: 954362

End: 955876

Strand: Direct

Name: purH [H]

Synonym: CHY_1078

Alternate gene names: 78043098

Gene position: 954362-955876 (Clockwise)

Preceding gene: 78045060

Following gene: 78044438

Centisome position: 39.74

GC content: 46.67

Gene sequence:

>1515_bases
ATGGGAAGTAAGCGGGCTTTAATTTCCGTTTACGATAAAAAGGGTATAGTGGAATTTGCCGGGAAATTAGTAGATTTAGG
CTTTGAAATAATTTCTACCGGGGGAAGTTATAAAGTTTTAAAAGAGGCCGGTATCCCGGTTACGTATATAAGTGATATTA
CCGGTTTTCCGGAAATTTTAGAAGGACGGGTAAAGACTTTGCATCCGGCAATTCACGGGGGTATCCTGGCCAAAAGAACG
GAGGAGCATTTAAAGGAAATTTCAGCCCAGGGTATAAAGCCTATTGATTTGGTGATAGTAAACCTGTATCCATTCCGGGA
AACGGTAGCGCGGGGAGCTGATCGTGACGAAATCATTGAAAATATTGACATAGGTGGCCCCACTTTAATCCGGGCAGCAG
CCAAAAACCACCGGTACGTTACGGTGGTGGTCGATCCGGAAGATTATGCGGAAGTGGCCGAACATCTGAAAAAGGGAGAG
GTGCCGCCTGAGGTCAGGGAAAGGTTAGCTTTAAAAGCGTTCCGCCATACTGCCAGTTACGACATTGCTATTGCCAATTA
TTTTGCGGGAAGGTTAGAGGATGGTGGTTTTCGGAAAAAATTATATATTGAAGCGGAACAGGTGGAAATGCTGCGGTACG
GAGAAAATCCCCACCAGCGGGGAGCGGTTTACCGGGTGGAGGGGACTTTTGGACTGGTTGATGCGGAAATTTTAAACGGT
AAAGCCATGAGCTTTAATAATTACCTTGATGCCGATGCAGCGTACCGGTTAATCTGGGAGTTTCGTGAGCCGGCGGCAGC
CATAATCAAGCACACCGTACCCTGCGGTGTGGCTACCGGGGAAAATTTAAAGGAAGCTTACGAAAAAGCGTATGCCGGTG
ATCCGGTGTCGGCTTACGGGGGAATTGTTGCTTTTAACGGTGCGGTTGATGAAAAGGTGGCTGAGCTTTTGGTTCAGCAT
TTTTACGAGGTAATAATTGCTCCGGACTTTTCCGATGAAGCCTTAAAAATCTTGCGGGAAAAGAAGAATTTACGGGTTAT
AAAGGCAAAACCGCTTGCTTTAGAGGAACTTAATTTAAGGCAAATTAGCGGAGGACTGTTGCTTCAGGAAAATGATGGGA
AGCTTTATGAAGAATTAAAGGTGGTAGCGGGTAATGATTTAACCGAAGGGCAAAGGGAAGATATAGTTTTTGGTTTAAAA
GTAGTGAAATACGTTAAATCCAATGCCATAGCTGTGGTAAAGGACGGGATGCTTTTAGGAGCTGGCGGTGGGCAGACCAG
CCGGATTGATGCGGTAAAAATTGCATTAAATAAAGCCGGGGAAAGAGCCCGGGGAGCGGTGGTGGTTTCCGATGCCTTCT
TCCCCTTTGCCGATTCCATCGAGGAACTGGCCAAAGCGGGGGTTTCGGTGGTAGTAGAGCCGGGAGGAGCGGTGCGCGAC
CGGGAAGTAATCGAAGAAGCGGCAAAAAGAAATGTAACTCTCGTTTTTACGGGAGTAAGGCATTTCTTACATTAG

Upstream 100 bases:

>100_bases
GAACACCGAATTTTGGTAGAGGCGCTTAGGCTTTTAAGTGAAAACCGCTTATTGGTAGAAGGACGGCGGGTAAGAATCTT
GCCGGACGGGGGTGAAGGAC

Downstream 100 bases:

>100_bases
GAGGGTTAGTGGATGAAGGTTCTGGTAGTGGGAAGTGGCGGCCGGGAGCATGCTATAGTAAAAAAACTTAAGGAAAGTCC
AAAAGTTACGGAGGTATTGG

Product: bifunctional purine biosynthesis protein PurH

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 504; Mature: 503

Protein sequence:

>504_residues
MGSKRALISVYDKKGIVEFAGKLVDLGFEIISTGGSYKVLKEAGIPVTYISDITGFPEILEGRVKTLHPAIHGGILAKRT
EEHLKEISAQGIKPIDLVIVNLYPFRETVARGADRDEIIENIDIGGPTLIRAAAKNHRYVTVVVDPEDYAEVAEHLKKGE
VPPEVRERLALKAFRHTASYDIAIANYFAGRLEDGGFRKKLYIEAEQVEMLRYGENPHQRGAVYRVEGTFGLVDAEILNG
KAMSFNNYLDADAAYRLIWEFREPAAAIIKHTVPCGVATGENLKEAYEKAYAGDPVSAYGGIVAFNGAVDEKVAELLVQH
FYEVIIAPDFSDEALKILREKKNLRVIKAKPLALEELNLRQISGGLLLQENDGKLYEELKVVAGNDLTEGQREDIVFGLK
VVKYVKSNAIAVVKDGMLLGAGGGQTSRIDAVKIALNKAGERARGAVVVSDAFFPFADSIEELAKAGVSVVVEPGGAVRD
REVIEEAAKRNVTLVFTGVRHFLH

Sequences:

>Translated_504_residues
MGSKRALISVYDKKGIVEFAGKLVDLGFEIISTGGSYKVLKEAGIPVTYISDITGFPEILEGRVKTLHPAIHGGILAKRT
EEHLKEISAQGIKPIDLVIVNLYPFRETVARGADRDEIIENIDIGGPTLIRAAAKNHRYVTVVVDPEDYAEVAEHLKKGE
VPPEVRERLALKAFRHTASYDIAIANYFAGRLEDGGFRKKLYIEAEQVEMLRYGENPHQRGAVYRVEGTFGLVDAEILNG
KAMSFNNYLDADAAYRLIWEFREPAAAIIKHTVPCGVATGENLKEAYEKAYAGDPVSAYGGIVAFNGAVDEKVAELLVQH
FYEVIIAPDFSDEALKILREKKNLRVIKAKPLALEELNLRQISGGLLLQENDGKLYEELKVVAGNDLTEGQREDIVFGLK
VVKYVKSNAIAVVKDGMLLGAGGGQTSRIDAVKIALNKAGERARGAVVVSDAFFPFADSIEELAKAGVSVVVEPGGAVRD
REVIEEAAKRNVTLVFTGVRHFLH
>Mature_503_residues
GSKRALISVYDKKGIVEFAGKLVDLGFEIISTGGSYKVLKEAGIPVTYISDITGFPEILEGRVKTLHPAIHGGILAKRTE
EHLKEISAQGIKPIDLVIVNLYPFRETVARGADRDEIIENIDIGGPTLIRAAAKNHRYVTVVVDPEDYAEVAEHLKKGEV
PPEVRERLALKAFRHTASYDIAIANYFAGRLEDGGFRKKLYIEAEQVEMLRYGENPHQRGAVYRVEGTFGLVDAEILNGK
AMSFNNYLDADAAYRLIWEFREPAAAIIKHTVPCGVATGENLKEAYEKAYAGDPVSAYGGIVAFNGAVDEKVAELLVQHF
YEVIIAPDFSDEALKILREKKNLRVIKAKPLALEELNLRQISGGLLLQENDGKLYEELKVVAGNDLTEGQREDIVFGLKV
VKYVKSNAIAVVKDGMLLGAGGGQTSRIDAVKIALNKAGERARGAVVVSDAFFPFADSIEELAKAGVSVVVEPGGAVRDR
EVIEEAAKRNVTLVFTGVRHFLH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=474, Percent_Identity=40.084388185654, Blast_Score=288, Evalue=1e-77,
Organism=Escherichia coli, GI1790439, Length=523, Percent_Identity=45.697896749522, Blast_Score=440, Evalue=1e-125,
Organism=Caenorhabditis elegans, GI71985564, Length=474, Percent_Identity=36.2869198312236, Blast_Score=271, Evalue=8e-73,
Organism=Caenorhabditis elegans, GI71985574, Length=304, Percent_Identity=30.2631578947368, Blast_Score=107, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6323768, Length=469, Percent_Identity=38.3795309168444, Blast_Score=286, Evalue=6e-78,
Organism=Saccharomyces cerevisiae, GI6323056, Length=469, Percent_Identity=38.3795309168444, Blast_Score=284, Evalue=2e-77,
Organism=Drosophila melanogaster, GI24649832, Length=471, Percent_Identity=37.3673036093418, Blast_Score=270, Evalue=2e-72,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 55167; Mature: 55036

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSKRALISVYDKKGIVEFAGKLVDLGFEIISTGGSYKVLKEAGIPVTYISDITGFPEIL
CCCCCCEEEEECCCCHHHHHHHHHHCCHHHEECCCCEEEHHHCCCCEEEEHHCCCCHHHH
EGRVKTLHPAIHGGILAKRTEEHLKEISAQGIKPIDLVIVNLYPFRETVARGADRDEIIE
HCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEEEEECCHHHHHHCCCCHHHHHH
NIDIGGPTLIRAAAKNHRYVTVVVDPEDYAEVAEHLKKGEVPPEVRERLALKAFRHTASY
HCCCCCHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCC
DIAIANYFAGRLEDGGFRKKLYIEAEQVEMLRYGENPHQRGAVYRVEGTFGLVDAEILNG
EEEEHHHHHCCCCCCCCCEEEEEEHHHHHHHHCCCCCCCCCCEEEEECCEEEEEHHHHCC
KAMSFNNYLDADAAYRLIWEFREPAAAIIKHTVPCGVATGENLKEAYEKAYAGDPVSAYG
CEEECCCCCCHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHCC
GIVAFNGAVDEKVAELLVQHFYEVIIAPDFSDEALKILREKKNLRVIKAKPLALEELNLR
CEEEECCCHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHCCCCEEEEECCCCHHHCCHH
QISGGLLLQENDGKLYEELKVVAGNDLTEGQREDIVFGLKVVKYVKSNAIAVVKDGMLLG
EECCCEEEECCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCEEEE
AGGGQTSRIDAVKIALNKAGERARGAVVVSDAFFPFADSIEELAKAGVSVVVEPGGAVRD
CCCCCCCCHHHEEEEHHHCCCCCCCEEEEECCCCCCHHHHHHHHHCCCEEEECCCCCCCH
REVIEEAAKRNVTLVFTGVRHFLH
HHHHHHHHHCCEEEEEECHHHHCC
>Mature Secondary Structure 
GSKRALISVYDKKGIVEFAGKLVDLGFEIISTGGSYKVLKEAGIPVTYISDITGFPEIL
CCCCCEEEEECCCCHHHHHHHHHHCCHHHEECCCCEEEHHHCCCCEEEEHHCCCCHHHH
EGRVKTLHPAIHGGILAKRTEEHLKEISAQGIKPIDLVIVNLYPFRETVARGADRDEIIE
HCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEEEEECCHHHHHHCCCCHHHHHH
NIDIGGPTLIRAAAKNHRYVTVVVDPEDYAEVAEHLKKGEVPPEVRERLALKAFRHTASY
HCCCCCHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCC
DIAIANYFAGRLEDGGFRKKLYIEAEQVEMLRYGENPHQRGAVYRVEGTFGLVDAEILNG
EEEEHHHHHCCCCCCCCCEEEEEEHHHHHHHHCCCCCCCCCCEEEEECCEEEEEHHHHCC
KAMSFNNYLDADAAYRLIWEFREPAAAIIKHTVPCGVATGENLKEAYEKAYAGDPVSAYG
CEEECCCCCCHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHCC
GIVAFNGAVDEKVAELLVQHFYEVIIAPDFSDEALKILREKKNLRVIKAKPLALEELNLR
CEEEECCCHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHCCCCEEEEECCCCHHHCCHH
QISGGLLLQENDGKLYEELKVVAGNDLTEGQREDIVFGLKVVKYVKSNAIAVVKDGMLLG
EECCCEEEECCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCEEEE
AGGGQTSRIDAVKIALNKAGERARGAVVVSDAFFPFADSIEELAKAGVSVVVEPGGAVRD
CCCCCCCCHHHEEEEHHHCCCCCCCEEEEECCCCCCHHHHHHHHHCCCEEEECCCCCCCH
REVIEEAAKRNVTLVFTGVRHFLH
HHHHHHHHHCCEEEEEECHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA