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The map label for this gene is purD [H]

Identifier: 78044438

GI number: 78044438

Start: 955889

End: 957157

Strand: Direct

Name: purD [H]

Synonym: CHY_1079

Alternate gene names: 78044438

Gene position: 955889-957157 (Clockwise)

Preceding gene: 78043098

Following gene: 78043299

Centisome position: 39.8

GC content: 51.38

Gene sequence:

>1269_bases
ATGAAGGTTCTGGTAGTGGGAAGTGGCGGCCGGGAGCATGCTATAGTAAAAAAACTTAAGGAAAGTCCAAAAGTTACGGA
GGTATTGGCCGCTCCGGGTAATGCCGGCATTGCCCGGGAGGCTCGGGTGGTGCCGGTGGAAGTGGATAACCTGTCCGGGC
TTTTGGAAATTGCCCTGCGGGAGAAAGTGGATTTAACGGTGGTGGGACCCGAGCTTCCCTTAACCCTGGGGATTGTCGAT
TATTTTAACGAACATGGTCTTTTAGTCTTTGGCCCAGGCCGGGATGCGGCCCAAATTGAGGGCAGTAAAAGTTTTGCCAA
GGAACTGATGCGGGAAAACGGTATTCCTACGGCTTTTTATGAAACTTTTACCGATTATAACGAAGCCAGGGAGTACGTTT
ACCGGAAAGGGGTGCCGATAGTCATTAAAGCCGACGGCCTGGCGGCGGGTAAAGGGGTTTACGTTTGCCAGGAGTTGGAG
GAAGCGATAAAAGCTTTAAACGATATTTTCGTTAAGCGGATTTTTGGGGCTGCGGGAAATAAAGTGGTCATTGAAGAATA
TCTCGAGGGGCAAGAGGTTAGCGTCCTCGCTTTTACCGATGGGGAAACGGTGGTGCAAATGGTGCCGGCACAGGACCATA
AAAGGGTGTTTAACAACGACCAGGGACCCAATACCGGGGGAATGGGAGCCTATGCCCCGGCTCCCGTATATACTCCGGAA
ATTGCCGAAAGGGTCCAGCGGGAAATCCTGCTGCCCACATTAAAGGGGCTTAAAGGGAAAGGAATAACCTACCGGGGAGT
TTTGTATGCGGGGCTTATCTTAACCCGGGAGGGGCCAAAAGTTTTGGAGTTTAATGCCCGCTTTGGTGATCCCGAAACCC
AGGTTATCCTGCCCCTGTTAAAAACCGATTTGGTAGAGATTATGCTGGCGGTGGTGGAAAAAAGGCTTTCCGAGATAAAC
ATCGAATTTCTGCCCAAGGCCTGTGCCACGGTAGTATTGGCCTCCGGCGGGTATCCCGGAAAATACGAAACCGGCAAAGT
TATCCATGGTCTTGACCGGTTACCGCCGGGGGTGGTGGCGTATCATGCCGGAACCGCTTTCAATGGGGATGATATTGTTA
CCGCCGGTGGCCGGGTATTAAATATAACGGCGGTAGGGGATACCCTCAAGGCGGCCGTAGAACTTGCCTATGAAGGAGTT
AAGGCCGTATCCTTTGAAGGGATGCACTACCGCACCGATATTGGCCAAAAAGCTTTGAAATTACAATAG

Upstream 100 bases:

>100_bases
AGCGGTGCGCGACCGGGAAGTAATCGAAGAAGCGGCAAAAAGAAATGTAACTCTCGTTTTTACGGGAGTAAGGCATTTCT
TACATTAGGAGGGTTAGTGG

Downstream 100 bases:

>100_bases
GGCTGTTATTCAATTCGATAAGTACTTACTTCTTCCGAAGCCATAGGTATAACCTCTATGGCTTCTTTATTTTTCGGATT
ATTTATCCTGTAAAAAGTTA

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MKVLVVGSGGREHAIVKKLKESPKVTEVLAAPGNAGIAREARVVPVEVDNLSGLLEIALREKVDLTVVGPELPLTLGIVD
YFNEHGLLVFGPGRDAAQIEGSKSFAKELMRENGIPTAFYETFTDYNEAREYVYRKGVPIVIKADGLAAGKGVYVCQELE
EAIKALNDIFVKRIFGAAGNKVVIEEYLEGQEVSVLAFTDGETVVQMVPAQDHKRVFNNDQGPNTGGMGAYAPAPVYTPE
IAERVQREILLPTLKGLKGKGITYRGVLYAGLILTREGPKVLEFNARFGDPETQVILPLLKTDLVEIMLAVVEKRLSEIN
IEFLPKACATVVLASGGYPGKYETGKVIHGLDRLPPGVVAYHAGTAFNGDDIVTAGGRVLNITAVGDTLKAAVELAYEGV
KAVSFEGMHYRTDIGQKALKLQ

Sequences:

>Translated_422_residues
MKVLVVGSGGREHAIVKKLKESPKVTEVLAAPGNAGIAREARVVPVEVDNLSGLLEIALREKVDLTVVGPELPLTLGIVD
YFNEHGLLVFGPGRDAAQIEGSKSFAKELMRENGIPTAFYETFTDYNEAREYVYRKGVPIVIKADGLAAGKGVYVCQELE
EAIKALNDIFVKRIFGAAGNKVVIEEYLEGQEVSVLAFTDGETVVQMVPAQDHKRVFNNDQGPNTGGMGAYAPAPVYTPE
IAERVQREILLPTLKGLKGKGITYRGVLYAGLILTREGPKVLEFNARFGDPETQVILPLLKTDLVEIMLAVVEKRLSEIN
IEFLPKACATVVLASGGYPGKYETGKVIHGLDRLPPGVVAYHAGTAFNGDDIVTAGGRVLNITAVGDTLKAAVELAYEGV
KAVSFEGMHYRTDIGQKALKLQ
>Mature_422_residues
MKVLVVGSGGREHAIVKKLKESPKVTEVLAAPGNAGIAREARVVPVEVDNLSGLLEIALREKVDLTVVGPELPLTLGIVD
YFNEHGLLVFGPGRDAAQIEGSKSFAKELMRENGIPTAFYETFTDYNEAREYVYRKGVPIVIKADGLAAGKGVYVCQELE
EAIKALNDIFVKRIFGAAGNKVVIEEYLEGQEVSVLAFTDGETVVQMVPAQDHKRVFNNDQGPNTGGMGAYAPAPVYTPE
IAERVQREILLPTLKGLKGKGITYRGVLYAGLILTREGPKVLEFNARFGDPETQVILPLLKTDLVEIMLAVVEKRLSEIN
IEFLPKACATVVLASGGYPGKYETGKVIHGLDRLPPGVVAYHAGTAFNGDDIVTAGGRVLNITAVGDTLKAAVELAYEGV
KAVSFEGMHYRTDIGQKALKLQ

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI28416899, Length=423, Percent_Identity=47.7541371158392, Blast_Score=385, Evalue=1e-107,
Organism=Homo sapiens, GI4503915, Length=423, Percent_Identity=47.7541371158392, Blast_Score=385, Evalue=1e-107,
Organism=Homo sapiens, GI209869995, Length=423, Percent_Identity=47.7541371158392, Blast_Score=385, Evalue=1e-107,
Organism=Homo sapiens, GI209869993, Length=423, Percent_Identity=47.7541371158392, Blast_Score=385, Evalue=1e-107,
Organism=Escherichia coli, GI1790438, Length=426, Percent_Identity=50.7042253521127, Blast_Score=410, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI17567511, Length=422, Percent_Identity=43.8388625592417, Blast_Score=321, Evalue=4e-88,
Organism=Saccharomyces cerevisiae, GI6321203, Length=440, Percent_Identity=44.0909090909091, Blast_Score=364, Evalue=1e-101,
Organism=Drosophila melanogaster, GI62471619, Length=431, Percent_Identity=47.0997679814385, Blast_Score=352, Evalue=3e-97,
Organism=Drosophila melanogaster, GI24582400, Length=431, Percent_Identity=47.0997679814385, Blast_Score=350, Evalue=1e-96,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 45488; Mature: 45488

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVLVVGSGGREHAIVKKLKESPKVTEVLAAPGNAGIAREARVVPVEVDNLSGLLEIALR
CEEEEECCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCCEEEEEEECCHHHHHHHHHH
EKVDLTVVGPELPLTLGIVDYFNEHGLLVFGPGRDAAQIEGSKSFAKELMRENGIPTAFY
CCCEEEEECCCCCEEEEHHHHHCCCCEEEEECCCCCCEECCCHHHHHHHHHHCCCCHHHH
ETFTDYNEAREYVYRKGVPIVIKADGLAAGKGVYVCQELEEAIKALNDIFVKRIFGAAGN
HHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
KVVIEEYLEGQEVSVLAFTDGETVVQMVPAQDHKRVFNNDQGPNTGGMGAYAPAPVYTPE
EEEEHHHHCCCCEEEEEEECCCEEEEECCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCHH
IAERVQREILLPTLKGLKGKGITYRGVLYAGLILTREGPKVLEFNARFGDPETQVILPLL
HHHHHHHHHHHHHHCCCCCCCEEEHHHEEEEEEEECCCCCEEEEECCCCCCCHHEEHHHH
KTDLVEIMLAVVEKRLSEINIEFLPKACATVVLASGGYPGKYETGKVIHGLDRLPPGVVA
HHHHHHHHHHHHHHHHHHCCHHHHCHHHHEEEEECCCCCCCCCCCHHHHHHHCCCCCEEE
YHAGTAFNGDDIVTAGGRVLNITAVGDTLKAAVELAYEGVKAVSFEGMHYRTDIGQKALK
EECCCCCCCCCEEECCCEEEEEEECCHHHHHHHHHHHHCCCEEEECCCEEECCCCCHHHC
LQ
CC
>Mature Secondary Structure
MKVLVVGSGGREHAIVKKLKESPKVTEVLAAPGNAGIAREARVVPVEVDNLSGLLEIALR
CEEEEECCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCCEEEEEEECCHHHHHHHHHH
EKVDLTVVGPELPLTLGIVDYFNEHGLLVFGPGRDAAQIEGSKSFAKELMRENGIPTAFY
CCCEEEEECCCCCEEEEHHHHHCCCCEEEEECCCCCCEECCCHHHHHHHHHHCCCCHHHH
ETFTDYNEAREYVYRKGVPIVIKADGLAAGKGVYVCQELEEAIKALNDIFVKRIFGAAGN
HHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
KVVIEEYLEGQEVSVLAFTDGETVVQMVPAQDHKRVFNNDQGPNTGGMGAYAPAPVYTPE
EEEEHHHHCCCCEEEEEEECCCEEEEECCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCHH
IAERVQREILLPTLKGLKGKGITYRGVLYAGLILTREGPKVLEFNARFGDPETQVILPLL
HHHHHHHHHHHHHHCCCCCCCEEEHHHEEEEEEEECCCCCEEEEECCCCCCCHHEEHHHH
KTDLVEIMLAVVEKRLSEINIEFLPKACATVVLASGGYPGKYETGKVIHGLDRLPPGVVA
HHHHHHHHHHHHHHHHHHCCHHHHCHHHHEEEEECCCCCCCCCCCHHHHHHHCCCCCEEE
YHAGTAFNGDDIVTAGGRVLNITAVGDTLKAAVELAYEGVKAVSFEGMHYRTDIGQKALK
EECCCCCCCCCEEECCCEEEEEEECCHHHHHHHHHHHHCCCEEEECCCEEECCCCCHHHC
LQ
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]