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The map label for this gene is purN [H]

Identifier: 78045060

GI number: 78045060

Start: 953743

End: 954372

Strand: Direct

Name: purN [H]

Synonym: CHY_1077

Alternate gene names: 78045060

Gene position: 953743-954372 (Clockwise)

Preceding gene: 78044305

Following gene: 78043098

Centisome position: 39.71

GC content: 48.41

Gene sequence:

>630_bases
ATGTTAAACTTAGGGGTTTTGGCTTCGGGACGGGGGAGTAACTTTCAGGCGATTATTGATGCGATTGCGTGGGGAGTTTT
GCCGGCAAAAATTAAAGTGCTGGTGACCGATAATCCTGAAGCTTACGCTATCGAACGGGCCAGGCGGGCAGGTATACCCT
GGCATTACTTTGACCCGAAAGGTTTTAAAAATAAAGAGGAATACGAAAAGGAGATAGTGAAAACCTTACTTTCCTATGAA
GTTGATACGGTGTGTCTTGCGGGCTACATGCGCTTAATTGGCAAACCTTTACTTTCTTCTTTTCCCATGAGGATTATTAA
TATCCATCCGGCGCTGCTACCGGCGTTCCCCGGGCTTCATGCCCAAAAGCAGGCTCTTGATTACGGGGTGAAAATTGCCG
GCTGTACCGTGCATTTTGTCGATGAGGGGATGGATACCGGCCCCATTATTTTGCAGGCAGCGGTTCCGGTGTACGATGAC
GACAGTGAGGAAAGCTTAAGTGAGCGGATCTTAGAACAGGAACACCGAATTTTGGTAGAGGCGCTTAGGCTTTTAAGTGA
AAACCGCTTATTGGTAGAAGGACGGCGGGTAAGAATCTTGCCGGACGGGGGTGAAGGACATGGGAAGTAA

Upstream 100 bases:

>100_bases
CAATTAAAACGTTAGAGAGTTTTAATTATAAGGCATGGGTAATTGGAGAAATTACCGCGGGGGAACGCAGGGTGATTATT
AATGAATAGGGAGGAAGAAA

Downstream 100 bases:

>100_bases
GCGGGCTTTAATTTCCGTTTACGATAAAAAGGGTATAGTGGAATTTGCCGGGAAATTAGTAGATTTAGGCTTTGAAATAA
TTTCTACCGGGGGAAGTTAT

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 209; Mature: 209

Protein sequence:

>209_residues
MLNLGVLASGRGSNFQAIIDAIAWGVLPAKIKVLVTDNPEAYAIERARRAGIPWHYFDPKGFKNKEEYEKEIVKTLLSYE
VDTVCLAGYMRLIGKPLLSSFPMRIINIHPALLPAFPGLHAQKQALDYGVKIAGCTVHFVDEGMDTGPIILQAAVPVYDD
DSEESLSERILEQEHRILVEALRLLSENRLLVEGRRVRILPDGGEGHGK

Sequences:

>Translated_209_residues
MLNLGVLASGRGSNFQAIIDAIAWGVLPAKIKVLVTDNPEAYAIERARRAGIPWHYFDPKGFKNKEEYEKEIVKTLLSYE
VDTVCLAGYMRLIGKPLLSSFPMRIINIHPALLPAFPGLHAQKQALDYGVKIAGCTVHFVDEGMDTGPIILQAAVPVYDD
DSEESLSERILEQEHRILVEALRLLSENRLLVEGRRVRILPDGGEGHGK
>Mature_209_residues
MLNLGVLASGRGSNFQAIIDAIAWGVLPAKIKVLVTDNPEAYAIERARRAGIPWHYFDPKGFKNKEEYEKEIVKTLLSYE
VDTVCLAGYMRLIGKPLLSSFPMRIINIHPALLPAFPGLHAQKQALDYGVKIAGCTVHFVDEGMDTGPIILQAAVPVYDD
DSEESLSERILEQEHRILVEALRLLSENRLLVEGRRVRILPDGGEGHGK

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=187, Percent_Identity=41.7112299465241, Blast_Score=161, Evalue=4e-40,
Organism=Homo sapiens, GI209869995, Length=187, Percent_Identity=41.7112299465241, Blast_Score=161, Evalue=4e-40,
Organism=Homo sapiens, GI209869993, Length=187, Percent_Identity=41.7112299465241, Blast_Score=161, Evalue=4e-40,
Organism=Escherichia coli, GI1788846, Length=189, Percent_Identity=40.7407407407407, Blast_Score=166, Evalue=1e-42,
Organism=Escherichia coli, GI1787483, Length=177, Percent_Identity=31.638418079096, Blast_Score=96, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17567511, Length=181, Percent_Identity=37.5690607734807, Blast_Score=127, Evalue=5e-30,
Organism=Saccharomyces cerevisiae, GI6320616, Length=204, Percent_Identity=30.8823529411765, Blast_Score=81, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24582400, Length=193, Percent_Identity=45.5958549222798, Blast_Score=175, Evalue=2e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 23197; Mature: 23197

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNLGVLASGRGSNFQAIIDAIAWGVLPAKIKVLVTDNPEAYAIERARRAGIPWHYFDPK
CCCCCEEECCCCCCHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHCCCCEEECCCC
GFKNKEEYEKEIVKTLLSYEVDTVCLAGYMRLIGKPLLSSFPMRIINIHPALLPAFPGLH
CCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCHHHHHHCCEEEEEECHHHHHCCCCCC
AQKQALDYGVKIAGCTVHFVDEGMDTGPIILQAAVPVYDDDSEESLSERILEQEHRILVE
HHHHHHHCCEEEEEEEEEEECCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHH
ALRLLSENRLLVEGRRVRILPDGGEGHGK
HHHHHCCCCEEEECCEEEEEECCCCCCCC
>Mature Secondary Structure
MLNLGVLASGRGSNFQAIIDAIAWGVLPAKIKVLVTDNPEAYAIERARRAGIPWHYFDPK
CCCCCEEECCCCCCHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHCCCCEEECCCC
GFKNKEEYEKEIVKTLLSYEVDTVCLAGYMRLIGKPLLSSFPMRIINIHPALLPAFPGLH
CCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCHHHHHHCCEEEEEECHHHHHCCCCCC
AQKQALDYGVKIAGCTVHFVDEGMDTGPIILQAAVPVYDDDSEESLSERILEQEHRILVE
HHHHHHHCCEEEEEEEEEEECCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHH
ALRLLSENRLLVEGRRVRILPDGGEGHGK
HHHHHCCCCEEEECCEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377 [H]