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The map label for this gene is tnsB [H]

Identifier: 77461941

GI number: 77461941

Start: 6414895

End: 6417066

Strand: Reverse

Name: tnsB [H]

Synonym: Pfl01_5724

Alternate gene names: 77461941

Gene position: 6417066-6414895 (Counterclockwise)

Preceding gene: 77461942

Following gene: 77461940

Centisome position: 99.67

GC content: 46.13

Gene sequence:

>2172_bases
ATGAGTATCCAAGTTAATGACGTGTACGAACCAGTAACCGAAGATGCAATAATAAGTACTTTATCTCGCGTGCTATGGGT
GGGTCATAATGAAGGCGTGATAGCCTTTATTGATATTTCATCAAGCTTGGCGAAAAAACCTTTTCTTGTGGGTCTTGATG
AATTTAAAAGGTCAGTTAAGTCTGGTGACGTCAAGCTGACGAAAATAAAAGTGCCTCAGTACATTCTTATTCTAGATGAA
GAACTCAGTACTGAGCAGAAACAAGATCGCGATAAAAAGTGGGAGATGATTGCACCTATTGTCAACCATGAAGTACCCGG
CTACGTATTTTTTCCGGGCGAACTCGAAGCGTTAGTAGCTGCACGAGCATTGGCACTAGGTGTGTACACAAAGAAAATCT
CCCGGCTGCTCCATCGATATTGGGTTAATGGTCAGGTCAGAAATTCTCTTTTAAAAGATTACTCTAAGGTTGGGAAAACA
ACTCGAATATTCGTAGAAGGAAAAAAGCCAGGGCGAAAAGCCACTTACCAGGGTGAGCTTGTAACTCAGTACAAGATGCT
AGAGTCCGTTGACTTTAAATGTATAAAAGCGGGATATGCGTTATTTGTAGACGACGAAGCTGCCTCTCGGCGTGCCGCCT
ACGACAAAATGCTGTCTATGTTTTACAAGGTCAAGGATCTATCTAAACATCCGGAATCTTTGGGAAAGCTGCTACCCACA
TCGGAAATCCCATCCTACCGGCAATTTATGCATCATGGGAAAAGGTTTTTTGATGAAACCACTACCGCTAGAGGGCGGAT
GGGAGAAAAAAAATGGCAGAAAGACGGTCGTGGATTAGAGGGAACTGTTAGGGACAATTTGCGAGGGCCGTGTCATCAGT
TTGAAATTGACTCAACGGTTGCCGATATTTATTTAGTTAACAGTTATTCGCGACACATGCTTATCGGGCGTCCGATCATT
TATGTTGTCATAGATAGCTACTCTGGGATGATTGTTGGGCTATATGTGGGGTTAGAGGGGCCAAGTTGGAACGGTGCTCG
GCAAGCGCTTTTCAATGCCTTCACCCCAAAGCAGAAGTTTTGCGCCATGAATGGTGTCAATATTAGCGAAGATGATTGGC
CATGCCATCATTTGCCTTATGAAGTGTTCGCCGACCGTGCTGAAATGCTCAGTGAAGGAGCGGAAGGCCTCGCATCTGGC
TTGGGAGTTGACATAGGGATTGCACCGCCTTTCAGACCAGACTGGAAGTCCATGGTGGAAAGTCGTTTCAATATCTTAAA
CAGCCTGTCTGGTATACGATGGATGCCAGGTGGAGTAGCGGCGCGTAATAGAGAGCGTGGAGAGCGGGATTATCGCTTGG
ATGCCACTATGAACATGCGAGAGTTTACCAAGACAATCATAAAATGCGTACTTCACTACAATCAGTTTAATCGACAACCG
GCACGGCTATCAAAACAGATGGTCGATGATCAGGTTGAGCCTACTCCAATTGCTATTTGGAATTGGGCCATTGAGAAAGA
TTTTATGAGGGACAATAACAAAAGCGAAGAGCTTGTTTATTTAAATTTACTTCCCAGAGATACAGGATCTGTCAGGAAGG
GCGGAATCTTGTTTCGGGGCATGCATTACGTTAGCGAGTATCTCACGCATAATAATTGGACTGCTATTGCGAGGAATCAA
GGTAGTTGGCCAATCGAGTGTTGGCATCTTCACGAGGCGGCAAACCATATTTGGATACAGGATTCCAATAAGCAATTTGT
GAGATGCGATTTACGTAAGTCAGATCGAAAATATGCTGATTATCGATCTGATGAAGTGTATGACATGCTTGAGGCATACC
GCCAGGTGCCTCCTTCACATGCAAGGGATGAACTCGAAAGCAGGGTCGCGCTGCTGGATGACTTTGAGGGGACGTTCAAC
ACTGCTGCTTTGGAAAAAAAACAGACCCCGGCGCCCAAATCCAAGGCGCAGGCCACTGGAAATATTCGCGAACAGCGCTC
AGAAGAACTGGCTCGTGAACGCGCCTCGGCTCCGATCCCAGACGGCGTTCTTCCAGCAAAATCCGCGGCGCAAGAACCCA
CAAAAACTGATCATGCCCACCATTTTGCTGGGGCGCGTGGTGCTGTGATCATCGACATGCTGAAGCGAATTCGGCCTGGT
AATAAGCCATGA

Upstream 100 bases:

>100_bases
TAGACTTGGCCATCGCACCTATACAAACAGGCCTTCCGTTACCCTTCTTTGAAGTAGTTGGTGCGGTGCAGTCGCAGCCT
TTCAGCTTTGGGGAAGCGAT

Downstream 100 bases:

>100_bases
CGAGCACTCCTACCTTTGCCAGTTACACGAAGCAGGTCATCTCTGACTACGCAGGCAACCCTCTCATTGAGGCACTTCCA
CCTATTTTGTCATGGACAGA

Product: Tn7-like transposition protein B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 723; Mature: 722

Protein sequence:

>723_residues
MSIQVNDVYEPVTEDAIISTLSRVLWVGHNEGVIAFIDISSSLAKKPFLVGLDEFKRSVKSGDVKLTKIKVPQYILILDE
ELSTEQKQDRDKKWEMIAPIVNHEVPGYVFFPGELEALVAARALALGVYTKKISRLLHRYWVNGQVRNSLLKDYSKVGKT
TRIFVEGKKPGRKATYQGELVTQYKMLESVDFKCIKAGYALFVDDEAASRRAAYDKMLSMFYKVKDLSKHPESLGKLLPT
SEIPSYRQFMHHGKRFFDETTTARGRMGEKKWQKDGRGLEGTVRDNLRGPCHQFEIDSTVADIYLVNSYSRHMLIGRPII
YVVIDSYSGMIVGLYVGLEGPSWNGARQALFNAFTPKQKFCAMNGVNISEDDWPCHHLPYEVFADRAEMLSEGAEGLASG
LGVDIGIAPPFRPDWKSMVESRFNILNSLSGIRWMPGGVAARNRERGERDYRLDATMNMREFTKTIIKCVLHYNQFNRQP
ARLSKQMVDDQVEPTPIAIWNWAIEKDFMRDNNKSEELVYLNLLPRDTGSVRKGGILFRGMHYVSEYLTHNNWTAIARNQ
GSWPIECWHLHEAANHIWIQDSNKQFVRCDLRKSDRKYADYRSDEVYDMLEAYRQVPPSHARDELESRVALLDDFEGTFN
TAALEKKQTPAPKSKAQATGNIREQRSEELARERASAPIPDGVLPAKSAAQEPTKTDHAHHFAGARGAVIIDMLKRIRPG
NKP

Sequences:

>Translated_723_residues
MSIQVNDVYEPVTEDAIISTLSRVLWVGHNEGVIAFIDISSSLAKKPFLVGLDEFKRSVKSGDVKLTKIKVPQYILILDE
ELSTEQKQDRDKKWEMIAPIVNHEVPGYVFFPGELEALVAARALALGVYTKKISRLLHRYWVNGQVRNSLLKDYSKVGKT
TRIFVEGKKPGRKATYQGELVTQYKMLESVDFKCIKAGYALFVDDEAASRRAAYDKMLSMFYKVKDLSKHPESLGKLLPT
SEIPSYRQFMHHGKRFFDETTTARGRMGEKKWQKDGRGLEGTVRDNLRGPCHQFEIDSTVADIYLVNSYSRHMLIGRPII
YVVIDSYSGMIVGLYVGLEGPSWNGARQALFNAFTPKQKFCAMNGVNISEDDWPCHHLPYEVFADRAEMLSEGAEGLASG
LGVDIGIAPPFRPDWKSMVESRFNILNSLSGIRWMPGGVAARNRERGERDYRLDATMNMREFTKTIIKCVLHYNQFNRQP
ARLSKQMVDDQVEPTPIAIWNWAIEKDFMRDNNKSEELVYLNLLPRDTGSVRKGGILFRGMHYVSEYLTHNNWTAIARNQ
GSWPIECWHLHEAANHIWIQDSNKQFVRCDLRKSDRKYADYRSDEVYDMLEAYRQVPPSHARDELESRVALLDDFEGTFN
TAALEKKQTPAPKSKAQATGNIREQRSEELARERASAPIPDGVLPAKSAAQEPTKTDHAHHFAGARGAVIIDMLKRIRPG
NKP
>Mature_722_residues
SIQVNDVYEPVTEDAIISTLSRVLWVGHNEGVIAFIDISSSLAKKPFLVGLDEFKRSVKSGDVKLTKIKVPQYILILDEE
LSTEQKQDRDKKWEMIAPIVNHEVPGYVFFPGELEALVAARALALGVYTKKISRLLHRYWVNGQVRNSLLKDYSKVGKTT
RIFVEGKKPGRKATYQGELVTQYKMLESVDFKCIKAGYALFVDDEAASRRAAYDKMLSMFYKVKDLSKHPESLGKLLPTS
EIPSYRQFMHHGKRFFDETTTARGRMGEKKWQKDGRGLEGTVRDNLRGPCHQFEIDSTVADIYLVNSYSRHMLIGRPIIY
VVIDSYSGMIVGLYVGLEGPSWNGARQALFNAFTPKQKFCAMNGVNISEDDWPCHHLPYEVFADRAEMLSEGAEGLASGL
GVDIGIAPPFRPDWKSMVESRFNILNSLSGIRWMPGGVAARNRERGERDYRLDATMNMREFTKTIIKCVLHYNQFNRQPA
RLSKQMVDDQVEPTPIAIWNWAIEKDFMRDNNKSEELVYLNLLPRDTGSVRKGGILFRGMHYVSEYLTHNNWTAIARNQG
SWPIECWHLHEAANHIWIQDSNKQFVRCDLRKSDRKYADYRSDEVYDMLEAYRQVPPSHARDELESRVALLDDFEGTFNT
AALEKKQTPAPKSKAQATGNIREQRSEELARERASAPIPDGVLPAKSAAQEPTKTDHAHHFAGARGAVIIDMLKRIRPGN
KP

Specific function: Sequence-specific, DNA-binding protein required for Tn7 transposition. Recognizes sequences necessary for recombination at both left and right ends of Tn7 and, together with tnsA, forms the transposase. TnsB executes the 5'-DNA strand breakage and joining

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 82317; Mature: 82186

Theoretical pI: Translated: 9.03; Mature: 9.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIQVNDVYEPVTEDAIISTLSRVLWVGHNEGVIAFIDISSSLAKKPFLVGLDEFKRSVK
CEEECCCCCCCHHHHHHHHHHHHHHEECCCCCEEEEEECCHHHCCCCEEEEHHHHHHHHH
SGDVKLTKIKVPQYILILDEELSTEQKQDRDKKWEMIAPIVNHEVPGYVFFPGELEALVA
CCCEEEEEEECCEEEEEECCHHCHHHHHHHCCHHHHHHHHHCCCCCCEEEECCHHHHHHH
ARALALGVYTKKISRLLHRYWVNGQVRNSLLKDYSKVGKTTRIFVEGKKPGRKATYQGEL
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHH
VTQYKMLESVDFKCIKAGYALFVDDEAASRRAAYDKMLSMFYKVKDLSKHPESLGKLLPT
HHHHHHHHHCCCCEEECCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCC
SEIPSYRQFMHHGKRFFDETTTARGRMGEKKWQKDGRGLEGTVRDNLRGPCHQFEIDSTV
CCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCHHHHCCCCCCCEEECCCCE
ADIYLVNSYSRHMLIGRPIIYVVIDSYSGMIVGLYVGLEGPSWNGARQALFNAFTPKQKF
EEEEEECCCCCCEEECCCEEEEEEECCCCEEEEEEEECCCCCCCHHHHHHHHHCCCHHHH
CAMNGVNISEDDWPCHHLPYEVFADRAEMLSEGAEGLASGLGVDIGIAPPFRPDWKSMVE
HCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHH
SRFNILNSLSGIRWMPGGVAARNRERGERDYRLDATMNMREFTKTIIKCVLHYNQFNRQP
HHHHHHHHCCCCEECCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCH
ARLSKQMVDDQVEPTPIAIWNWAIEKDFMRDNNKSEELVYLNLLPRDTGSVRKGGILFRG
HHHHHHHHHCCCCCCCEEEEEHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCCHHHH
MHYVSEYLTHNNWTAIARNQGSWPIECWHLHEAANHIWIQDSNKQFVRCDLRKSDRKYAD
HHHHHHHHHCCCCEEEEECCCCCCEEEEEEHHHCCEEEEECCCCCEEEEECCCCCCHHHC
YRSDEVYDMLEAYRQVPPSHARDELESRVALLDDFEGTFNTAALEKKQTPAPKSKAQATG
CCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCHHHHHCC
NIREQRSEELARERASAPIPDGVLPAKSAAQEPTKTDHAHHFAGARGAVIIDMLKRIRPG
CHHHHHHHHHHHHHCCCCCCCCCCCCCHHCCCCCCCCCHHHHCCCCCHHHHHHHHHHCCC
NKP
CCC
>Mature Secondary Structure 
SIQVNDVYEPVTEDAIISTLSRVLWVGHNEGVIAFIDISSSLAKKPFLVGLDEFKRSVK
EEECCCCCCCHHHHHHHHHHHHHHEECCCCCEEEEEECCHHHCCCCEEEEHHHHHHHHH
SGDVKLTKIKVPQYILILDEELSTEQKQDRDKKWEMIAPIVNHEVPGYVFFPGELEALVA
CCCEEEEEEECCEEEEEECCHHCHHHHHHHCCHHHHHHHHHCCCCCCEEEECCHHHHHHH
ARALALGVYTKKISRLLHRYWVNGQVRNSLLKDYSKVGKTTRIFVEGKKPGRKATYQGEL
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHH
VTQYKMLESVDFKCIKAGYALFVDDEAASRRAAYDKMLSMFYKVKDLSKHPESLGKLLPT
HHHHHHHHHCCCCEEECCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCC
SEIPSYRQFMHHGKRFFDETTTARGRMGEKKWQKDGRGLEGTVRDNLRGPCHQFEIDSTV
CCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCHHHHCCCCCCCEEECCCCE
ADIYLVNSYSRHMLIGRPIIYVVIDSYSGMIVGLYVGLEGPSWNGARQALFNAFTPKQKF
EEEEEECCCCCCEEECCCEEEEEEECCCCEEEEEEEECCCCCCCHHHHHHHHHCCCHHHH
CAMNGVNISEDDWPCHHLPYEVFADRAEMLSEGAEGLASGLGVDIGIAPPFRPDWKSMVE
HCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHH
SRFNILNSLSGIRWMPGGVAARNRERGERDYRLDATMNMREFTKTIIKCVLHYNQFNRQP
HHHHHHHHCCCCEECCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCH
ARLSKQMVDDQVEPTPIAIWNWAIEKDFMRDNNKSEELVYLNLLPRDTGSVRKGGILFRG
HHHHHHHHHCCCCCCCEEEEEHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCCHHHH
MHYVSEYLTHNNWTAIARNQGSWPIECWHLHEAANHIWIQDSNKQFVRCDLRKSDRKYAD
HHHHHHHHHCCCCEEEEECCCCCCEEEEEEHHHCCEEEEECCCCCEEEEECCCCCCHHHC
YRSDEVYDMLEAYRQVPPSHARDELESRVALLDDFEGTFNTAALEKKQTPAPKSKAQATG
CCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCHHHHHCC
NIREQRSEELARERASAPIPDGVLPAKSAAQEPTKTDHAHHFAGARGAVIIDMLKRIRPG
CHHHHHHHHHHHHHCCCCCCCCCCCCCHHCCCCCCCCCHHHHCCCCCHHHHHHHHHHCCC
NKP
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2156235; 1655576; 1657979; 8947057; 10704304 [H]