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The map label for this gene is tnsA [H]

Identifier: 77461942

GI number: 77461942

Start: 6417063

End: 6417950

Strand: Reverse

Name: tnsA [H]

Synonym: Pfl01_5725

Alternate gene names: 77461942

Gene position: 6417950-6417063 (Counterclockwise)

Preceding gene: 77461943

Following gene: 77461941

Centisome position: 99.68

GC content: 46.73

Gene sequence:

>888_bases
GTGAAGCTATGCGATGCGGCGGGGGCTCAGGAGGTCCAGTTGCGCGGTCGCAGATTTGCTACAGAAGCTGACATCAGTCG
GCATATCTCCAACGGTTTCGGAGGCGGTGCCGGAAGCAGTTACATCCCATGGTTGCGCGTGCAGGATGTGCCATCGCTCG
GCCATTCTCGAAAGGTCCACGGCGTTAAAGTCGATCGCATTCATCACCTTCTCTCCAATCTTGAACACGGTGCCTTTCTG
GCCGCAGAATTCTCGGAAGTGGTCGTAGACATCAGAGAGCAGTTCCCTCTCTTGCCGCGTGGAACCGCCCAAGCAATAGC
GAGCACTTTGGGTATTCGCTATCCGCTATACCCAAAAACTTCATTACCTTTAGTTATGACTACGGATTTACTGCTTACCG
TGCGAGAGGCTGATGGGAACCATAAACAAGTGGCCTGGTCGGTAAAATACACCAAAGATTTAGATGATCTAAGAACAGTT
GAAAAGTTGGAAATAGAAAAAGAATTCTGGAATTCGCAAGGAGTGGCGTGGTCAATTGTGACTGAGGAGCAGTTTACTAT
TGATCTAATTAACAATTTAACAGTTCTTAGAAAGTATGCAGACCTTCCTAAGGCGCTGCTTCAATCCGATTTGATTGAAG
GTTTTTTATATTATTTAAAGCAGTGTAGTGGTTACGGCTGGCGAACCGGTGTCGCGCTAAGAAAAATTGCAGCTAAGCTT
TACATTACTTATGGTGATGCAAAAATCCTATACCATTATTTGATTTGGTCAAAGCGAATCAAAATAGACTTGGCCATCGC
ACCTATACAAACAGGCCTTCCGTTACCCTTCTTTGAAGTAGTTGGTGCGGTGCAGTCGCAGCCTTTCAGCTTTGGGGAAG
CGATATGA

Upstream 100 bases:

>100_bases
TGTCCGGATCTGCACCTGTGTTGCGTTGAGGGAGTAGGCTTGATGGCTGGAGCCTTTCTGAGGGATGAGCTCATCAGTTT
CCTAAGCTTATTTTTTTGGG

Downstream 100 bases:

>100_bases
GTATCCAAGTTAATGACGTGTACGAACCAGTAACCGAAGATGCAATAATAAGTACTTTATCTCGCGTGCTATGGGTGGGT
CATAATGAAGGCGTGATAGC

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MKLCDAAGAQEVQLRGRRFATEADISRHISNGFGGGAGSSYIPWLRVQDVPSLGHSRKVHGVKVDRIHHLLSNLEHGAFL
AAEFSEVVVDIREQFPLLPRGTAQAIASTLGIRYPLYPKTSLPLVMTTDLLLTVREADGNHKQVAWSVKYTKDLDDLRTV
EKLEIEKEFWNSQGVAWSIVTEEQFTIDLINNLTVLRKYADLPKALLQSDLIEGFLYYLKQCSGYGWRTGVALRKIAAKL
YITYGDAKILYHYLIWSKRIKIDLAIAPIQTGLPLPFFEVVGAVQSQPFSFGEAI

Sequences:

>Translated_295_residues
MKLCDAAGAQEVQLRGRRFATEADISRHISNGFGGGAGSSYIPWLRVQDVPSLGHSRKVHGVKVDRIHHLLSNLEHGAFL
AAEFSEVVVDIREQFPLLPRGTAQAIASTLGIRYPLYPKTSLPLVMTTDLLLTVREADGNHKQVAWSVKYTKDLDDLRTV
EKLEIEKEFWNSQGVAWSIVTEEQFTIDLINNLTVLRKYADLPKALLQSDLIEGFLYYLKQCSGYGWRTGVALRKIAAKL
YITYGDAKILYHYLIWSKRIKIDLAIAPIQTGLPLPFFEVVGAVQSQPFSFGEAI
>Mature_295_residues
MKLCDAAGAQEVQLRGRRFATEADISRHISNGFGGGAGSSYIPWLRVQDVPSLGHSRKVHGVKVDRIHHLLSNLEHGAFL
AAEFSEVVVDIREQFPLLPRGTAQAIASTLGIRYPLYPKTSLPLVMTTDLLLTVREADGNHKQVAWSVKYTKDLDDLRTV
EKLEIEKEFWNSQGVAWSIVTEEQFTIDLINNLTVLRKYADLPKALLQSDLIEGFLYYLKQCSGYGWRTGVALRKIAAKL
YITYGDAKILYHYLIWSKRIKIDLAIAPIQTGLPLPFFEVVGAVQSQPFSFGEAI

Specific function: Required for Tn7 transposition. Forms the transposase, together with TnsB. TnsA executes the 5'-DNA strand breakage reaction. TnsABC and tnsD promote high-frequency insertion of Tn7 into a specific target site known as ATT-Tn7 whereas tnsABC and tnsD prom

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011856
- InterPro:   IPR011335
- InterPro:   IPR014832
- InterPro:   IPR014833
- InterPro:   IPR011991 [H]

Pfam domain/function: PF08721 TnsA_C; PF08722 TnsA_N [H]

EC number: NA

Molecular weight: Translated: 33060; Mature: 33060

Theoretical pI: Translated: 8.59; Mature: 8.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLCDAAGAQEVQLRGRRFATEADISRHISNGFGGGAGSSYIPWLRVQDVPSLGHSRKVH
CCCCCCCCCHHHHHCCCHHCHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCCCCCCC
GVKVDRIHHLLSNLEHGAFLAAEFSEVVVDIREQFPLLPRGTAQAIASTLGIRYPLYPKT
CCHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCC
SLPLVMTTDLLLTVREADGNHKQVAWSVKYTKDLDDLRTVEKLEIEKEFWNSQGVAWSIV
CCCEEEECCEEEEEECCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCEEEEE
TEEQFTIDLINNLTVLRKYADLPKALLQSDLIEGFLYYLKQCSGYGWRTGVALRKIAAKL
ECCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHEE
YITYGDAKILYHYLIWSKRIKIDLAIAPIQTGLPLPFFEVVGAVQSQPFSFGEAI
EEEECCHHHHHHHHHHHCEEEEEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKLCDAAGAQEVQLRGRRFATEADISRHISNGFGGGAGSSYIPWLRVQDVPSLGHSRKVH
CCCCCCCCCHHHHHCCCHHCHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCCCCCCC
GVKVDRIHHLLSNLEHGAFLAAEFSEVVVDIREQFPLLPRGTAQAIASTLGIRYPLYPKT
CCHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCC
SLPLVMTTDLLLTVREADGNHKQVAWSVKYTKDLDDLRTVEKLEIEKEFWNSQGVAWSIV
CCCEEEECCEEEEEECCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCEEEEE
TEEQFTIDLINNLTVLRKYADLPKALLQSDLIEGFLYYLKQCSGYGWRTGVALRKIAAKL
ECCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHEE
YITYGDAKILYHYLIWSKRIKIDLAIAPIQTGLPLPFFEVVGAVQSQPFSFGEAI
EEEECCHHHHHHHHHHHCEEEEEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2156235; 1655576; 3010949; 8947057; 10704304 [H]