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The map label for this gene is emrB [H]

Identifier: 77460016

GI number: 77460016

Start: 4295606

End: 4297198

Strand: Direct

Name: emrB [H]

Synonym: Pfl01_3794

Alternate gene names: 77460016

Gene position: 4295606-4297198 (Clockwise)

Preceding gene: 77460015

Following gene: 77460018

Centisome position: 66.72

GC content: 62.65

Gene sequence:

>1593_bases
ATGAGCACCGCCCTCACCGCCGCCGCGCAGCCATTCAATGCCGCCGAGATGGCGACGGCGACCAAGGTGTTCGCCTTCGC
CACAATGTGCATCGGCATGTTCATCGCGTTGCTGGACATCCAGATCGTCTCGGCCTCGCTGCGCGATATCGGCGGCGGGT
TGTCCGCCGGCACCGATGAAACGGCGTGGGTGCAGACCAGTTATCTGATCGCCGAGATCATCGTGATTCCACTGTCGGGT
TGGCTGTCGCGGGTTTTTTCCACGCGCTGGCTGTTCTGCGCCTCGGCGGTCGGCTTCACCCTCGCCAGCCTGCTCTGCGG
CATCGCCTGGAACATCCAGAGCATGATCGCCTTTCGCGCGCTGCAAGGGTTTCTCGGTGGGTCGATGATTCCGCTGGTGT
TCACCACGGCGTTCTTTTTCTTCACCGGCAAGCAGCGAGTGATTGCTGCCGCGACCATCGGCGCCGTGGCTTCGCTGGCA
CCGACGTTGGGCCCGGTGATCGGCGGCTGGATCACCGATATTTCGTCCTGGCACTGGCTATTCTACATCAATCTGGTGCC
GGGGATTTTCGTCGCCGTGGCGGTGCCGATGCTGGTGAAGATCGACCAGCCGGAACTGTCGCTGCTCAAAGGCGCGGACT
ATTTGAGCATGGTGTTTCTGGCGCTGTTTCTCGGCTGCCTGGAATACACCCTCGAAGAAGGCCCGCGCTGGAACTGGTTC
AGCGACCAGACGATCCTGACCACCGCGTGGATCAGTGGCCTGGCCGGGCTGGCCTTCGTCGGCCGCACGCTGCACGTCGC
CAATCCGATCGTCGACCTGCGCGCGCTCAAGGATCGCAACTTCGCCCTTGGCTGCTTCTTTTCCTTCGTCACCGGCATCG
GCCTGTTCGCGACGATTTACCTGACGCCGCTGTTTCTCGGCCGGGTACGCGGCTACGGCGCGCTGGATATTGGTCTGGCA
GTTTTCTCCACCGGGGTGTTCCAGATCATGGCGATTCCGCTGTACGCCTTTCTCGCCAATCGCATCGATCTGCGCTGGAT
CATGATGGCGGGCCTGGCGTTGTTTGCCTTGTCGATGTGGGAATTCAGTCCGATCAACCACGATTGGGGCGCCGGAGAAT
TGCTGCTGCCGCAAGCCCTGCGCGGGATTGCCCAGCAACTGGCGGTGCCGCCGGCGGTGACATTGACTTTAGGGGGCTTG
GCACCGGCGCGGCTCAGGCATGCGTCGGGCCTGTTCAACCTGATGCGCAATCTGGGCGGTGCTATCGGGATTGCCGCCTG
CGCGACCATCCTCAATGACCGCACCAACCTGCATTTCACCCGGTTGGCGGAGCATCTGAACAGCAGCAACGAAGCGGTGA
ATCAGTGGCTGGCCCAGGTCGGCGGCAACTTTGCCGCGCTGGGTCAGAGCGGCGACGTCGGCCTCACCGCCAGCCTGCGT
CAGCTATGGCTGCTGACCTACCGTGAAGCGCAGACGCAAACCTACGGCGACACGTTCCTGATGATAATGGTGTGCTTCGG
CATCGCCACGGCGATGGTGCCCTTGATGCGCAAGGTGCAACCACCGGCCGCGCCGAGTGCCGATGCACATTGA

Upstream 100 bases:

>100_bases
CGGCGGACGGCGTGCTCGGCCATCTGCGTCCCGGCCTGTCGGTGACCGCTGAAGTCGACACCCGCGCCCAACCTGAAACC
ACTGCCGTGGCCAGCGCGCC

Downstream 100 bases:

>100_bases
TCGATCAGGCTTGCGGAGTTTTACGGAAGCCCACGGCCAGGCGGTTCCAGCTGTTGATGGTGCTGATTGCGACCGTCAGG
TCGACCATTTCCTGAGGCGA

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 530; Mature: 529

Protein sequence:

>530_residues
MSTALTAAAQPFNAAEMATATKVFAFATMCIGMFIALLDIQIVSASLRDIGGGLSAGTDETAWVQTSYLIAEIIVIPLSG
WLSRVFSTRWLFCASAVGFTLASLLCGIAWNIQSMIAFRALQGFLGGSMIPLVFTTAFFFFTGKQRVIAAATIGAVASLA
PTLGPVIGGWITDISSWHWLFYINLVPGIFVAVAVPMLVKIDQPELSLLKGADYLSMVFLALFLGCLEYTLEEGPRWNWF
SDQTILTTAWISGLAGLAFVGRTLHVANPIVDLRALKDRNFALGCFFSFVTGIGLFATIYLTPLFLGRVRGYGALDIGLA
VFSTGVFQIMAIPLYAFLANRIDLRWIMMAGLALFALSMWEFSPINHDWGAGELLLPQALRGIAQQLAVPPAVTLTLGGL
APARLRHASGLFNLMRNLGGAIGIAACATILNDRTNLHFTRLAEHLNSSNEAVNQWLAQVGGNFAALGQSGDVGLTASLR
QLWLLTYREAQTQTYGDTFLMIMVCFGIATAMVPLMRKVQPPAAPSADAH

Sequences:

>Translated_530_residues
MSTALTAAAQPFNAAEMATATKVFAFATMCIGMFIALLDIQIVSASLRDIGGGLSAGTDETAWVQTSYLIAEIIVIPLSG
WLSRVFSTRWLFCASAVGFTLASLLCGIAWNIQSMIAFRALQGFLGGSMIPLVFTTAFFFFTGKQRVIAAATIGAVASLA
PTLGPVIGGWITDISSWHWLFYINLVPGIFVAVAVPMLVKIDQPELSLLKGADYLSMVFLALFLGCLEYTLEEGPRWNWF
SDQTILTTAWISGLAGLAFVGRTLHVANPIVDLRALKDRNFALGCFFSFVTGIGLFATIYLTPLFLGRVRGYGALDIGLA
VFSTGVFQIMAIPLYAFLANRIDLRWIMMAGLALFALSMWEFSPINHDWGAGELLLPQALRGIAQQLAVPPAVTLTLGGL
APARLRHASGLFNLMRNLGGAIGIAACATILNDRTNLHFTRLAEHLNSSNEAVNQWLAQVGGNFAALGQSGDVGLTASLR
QLWLLTYREAQTQTYGDTFLMIMVCFGIATAMVPLMRKVQPPAAPSADAH
>Mature_529_residues
STALTAAAQPFNAAEMATATKVFAFATMCIGMFIALLDIQIVSASLRDIGGGLSAGTDETAWVQTSYLIAEIIVIPLSGW
LSRVFSTRWLFCASAVGFTLASLLCGIAWNIQSMIAFRALQGFLGGSMIPLVFTTAFFFFTGKQRVIAAATIGAVASLAP
TLGPVIGGWITDISSWHWLFYINLVPGIFVAVAVPMLVKIDQPELSLLKGADYLSMVFLALFLGCLEYTLEEGPRWNWFS
DQTILTTAWISGLAGLAFVGRTLHVANPIVDLRALKDRNFALGCFFSFVTGIGLFATIYLTPLFLGRVRGYGALDIGLAV
FSTGVFQIMAIPLYAFLANRIDLRWIMMAGLALFALSMWEFSPINHDWGAGELLLPQALRGIAQQLAVPPAVTLTLGGLA
PARLRHASGLFNLMRNLGGAIGIAACATILNDRTNLHFTRLAEHLNSSNEAVNQWLAQVGGNFAALGQSGDVGLTASLRQ
LWLLTYREAQTQTYGDTFLMIMVCFGIATAMVPLMRKVQPPAAPSADAH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=457, Percent_Identity=30.6345733041575, Blast_Score=212, Evalue=6e-56,
Organism=Escherichia coli, GI1788710, Length=450, Percent_Identity=30, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI1790195, Length=418, Percent_Identity=24.1626794258373, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI87081983, Length=428, Percent_Identity=27.1028037383178, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1788392, Length=330, Percent_Identity=26.969696969697, Blast_Score=86, Evalue=7e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 57042; Mature: 56910

Theoretical pI: Translated: 7.47; Mature: 7.47

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
STALTAAAQPFNAAEMATATKVFAFATMCIGMFIALLDIQIVSASLRDIGGGLSAGTDE
CCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC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HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]