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The map label for this gene is fdoH [H]

Identifier: 77458924

GI number: 77458924

Start: 3109991

End: 3110926

Strand: Direct

Name: fdoH [H]

Synonym: Pfl01_2699

Alternate gene names: 77458924

Gene position: 3109991-3110926 (Clockwise)

Preceding gene: 77458923

Following gene: 77458925

Centisome position: 48.3

GC content: 64.1

Gene sequence:

>936_bases
ATGGCCAGCCAAGACATCATTGCCCGCTCGGCCACCACCACGCCGGCGCCTTCGATACGCGGCCAGGAACAGGTGGCCAA
GCTGATCGACACCACCAAATGCATCGGCTGCAAGGCCTGCCAGGTCGCGTGCTCGGAATGGAACGAGCTGCGCGACGAGG
TCGGCCACAACCACGGCACCTACGACAACCCGCAGGATTTGAGCGCCGATTCATGGACGCTGATGCGCTTCACCGAGCAT
GAAACCGACGCCGGTAACCTCGAGTGGCTGATCCGCAAGGACGGCTGCATGCACTGCGCCGAACCCGGTTGCCTGGCAGC
GTGCCCCAGCCCCGGCGCGATCATCAAGCACGCCAACGGCATCGTCGATTTCGATCAGGATCACTGCATCGGCTGCGGTT
ATTGCATCACCGGTTGCCCGTTCAACATCCCGCGCATTTCGCAGAAGGATCACAAGGCCTACAAATGCACGCTGTGTTCG
GACCGTGTGGCGGTGGGGCTGGAGCCGGCCTGCGTGAAAACCTGCCCGACCGGGGCCATCGTGTTTGGCACCAAGGACGA
CATGAAGACCCACGCCGCCGAACGCATCGTCGACCTGAAAAGCCGTGGCTTCGACAACGCCGGCCTGTATGACCCGCAAG
GCGTCGGCGGCACCCACGTCATGTACGTGCTGCACCACGCCGACACCCCGAAAATCTACGCCGGCCTGCCGGACAACCCC
GAGATCAGTCCGCTGGTGGGTTTGTGGAAAGGCATCAGCAAGCCGTTGGGTCTGCTGGCCATGGGCGCGGCGGTGCTGGC
CGGGTTCTTCCATTACGTGCGGATCGGCCCGAACCGGGTCGAGGAAGATGAACATCCCGATCCGCCCGACACCTCGGTGC
ACGTCGTCGATCCGGCAGTGCACACCTTCGACCCACGCGGGGAGGGCCGGCCATGA

Upstream 100 bases:

>100_bases
CCAACACCCTGGTGCCGTTTCTCGGCGATGGCAATACACAGACCCCGGAATCCAAGTCATTCCTGGTCAACGTGGAGAAG
CTCTGATGGAGAATCTTTAG

Downstream 100 bases:

>100_bases
GCAACAAGACGATCCTGCGCTACACCGCCAACCAGCGCACCAATCACTGGCTGGTGGCGATTCTGTTCTTCATGGCCGGG
CTGTCGGGGCTGGCGCTGTT

Product: formate dehydrogenase subunit beta

Products: CO2; NADH

Alternate protein names: Aerobic formate dehydrogenase iron-sulfur subunit; FDH-Z subunit beta; Formate dehydrogenase-O subunit beta [H]

Number of amino acids: Translated: 311; Mature: 310

Protein sequence:

>311_residues
MASQDIIARSATTTPAPSIRGQEQVAKLIDTTKCIGCKACQVACSEWNELRDEVGHNHGTYDNPQDLSADSWTLMRFTEH
ETDAGNLEWLIRKDGCMHCAEPGCLAACPSPGAIIKHANGIVDFDQDHCIGCGYCITGCPFNIPRISQKDHKAYKCTLCS
DRVAVGLEPACVKTCPTGAIVFGTKDDMKTHAAERIVDLKSRGFDNAGLYDPQGVGGTHVMYVLHHADTPKIYAGLPDNP
EISPLVGLWKGISKPLGLLAMGAAVLAGFFHYVRIGPNRVEEDEHPDPPDTSVHVVDPAVHTFDPRGEGRP

Sequences:

>Translated_311_residues
MASQDIIARSATTTPAPSIRGQEQVAKLIDTTKCIGCKACQVACSEWNELRDEVGHNHGTYDNPQDLSADSWTLMRFTEH
ETDAGNLEWLIRKDGCMHCAEPGCLAACPSPGAIIKHANGIVDFDQDHCIGCGYCITGCPFNIPRISQKDHKAYKCTLCS
DRVAVGLEPACVKTCPTGAIVFGTKDDMKTHAAERIVDLKSRGFDNAGLYDPQGVGGTHVMYVLHHADTPKIYAGLPDNP
EISPLVGLWKGISKPLGLLAMGAAVLAGFFHYVRIGPNRVEEDEHPDPPDTSVHVVDPAVHTFDPRGEGRP
>Mature_310_residues
ASQDIIARSATTTPAPSIRGQEQVAKLIDTTKCIGCKACQVACSEWNELRDEVGHNHGTYDNPQDLSADSWTLMRFTEHE
TDAGNLEWLIRKDGCMHCAEPGCLAACPSPGAIIKHANGIVDFDQDHCIGCGYCITGCPFNIPRISQKDHKAYKCTLCSD
RVAVGLEPACVKTCPTGAIVFGTKDDMKTHAAERIVDLKSRGFDNAGLYDPQGVGGTHVMYVLHHADTPKIYAGLPDNPE
ISPLVGLWKGISKPLGLLAMGAAVLAGFFHYVRIGPNRVEEDEHPDPPDTSVHVVDPAVHTFDPRGEGRP

Specific function: Allows to use formate as major electron donor during aerobic respiration. The beta chain is an electron transfer unit containing 4 cysteine clusters involved in the formation of iron- sulfur centers. Electrons are transferred from the gamma chain to the m

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1790326, Length=286, Percent_Identity=69.2307692307692, Blast_Score=419, Evalue=1e-118,
Organism=Escherichia coli, GI1787749, Length=290, Percent_Identity=63.7931034482759, Blast_Score=391, Evalue=1e-110,
Organism=Escherichia coli, GI1789370, Length=300, Percent_Identity=28.6666666666667, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1787122, Length=166, Percent_Identity=34.3373493975904, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1787872, Length=166, Percent_Identity=33.1325301204819, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI2367345, Length=168, Percent_Identity=31.547619047619, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI226510944, Length=194, Percent_Identity=28.3505154639175, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1787478, Length=173, Percent_Identity=29.4797687861272, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787740, Length=112, Percent_Identity=34.8214285714286, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI87082179, Length=174, Percent_Identity=29.8850574712644, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787963, Length=148, Percent_Identity=25, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1788811, Length=175, Percent_Identity=25.7142857142857, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI87082180, Length=155, Percent_Identity=30.3225806451613, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI2367245, Length=166, Percent_Identity=31.3253012048193, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006470
- InterPro:   IPR014603
- InterPro:   IPR015246 [H]

Pfam domain/function: PF09163 Form-deh_trans [H]

EC number: 1.2.1.2

Molecular weight: Translated: 33610; Mature: 33479

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
5.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASQDIIARSATTTPAPSIRGQEQVAKLIDTTKCIGCKACQVACSEWNELRDEVGHNHGT
CCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCC
YDNPQDLSADSWTLMRFTEHETDAGNLEWLIRKDGCMHCAEPGCLAACPSPGAIIKHANG
CCCCCCCCCCCEEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCEEECCCCCCEEECCCC
IVDFDQDHCIGCGYCITGCPFNIPRISQKDHKAYKCTLCSDRVAVGLEPACVKTCPTGAI
CEECCCCCCEECCHHHCCCCCCCCCCCCCCCCEEEEEEECCCEEECCCHHHHHHCCCCEE
VFGTKDDMKTHAAERIVDLKSRGFDNAGLYDPQGVGGTHVMYVLHHADTPKIYAGLPDNP
EEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEECCCCEEEECCCCCC
EISPLVGLWKGISKPLGLLAMGAAVLAGFFHYVRIGPNRVEEDEHPDPPDTSVHVVDPAV
CCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCHH
HTFDPRGEGRP
CCCCCCCCCCC
>Mature Secondary Structure 
ASQDIIARSATTTPAPSIRGQEQVAKLIDTTKCIGCKACQVACSEWNELRDEVGHNHGT
CCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCC
YDNPQDLSADSWTLMRFTEHETDAGNLEWLIRKDGCMHCAEPGCLAACPSPGAIIKHANG
CCCCCCCCCCCEEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCEEECCCCCCEEECCCC
IVDFDQDHCIGCGYCITGCPFNIPRISQKDHKAYKCTLCSDRVAVGLEPACVKTCPTGAI
CEECCCCCCEECCHHHCCCCCCCCCCCCCCCCEEEEEEECCCEEECCCHHHHHHCCCCEE
VFGTKDDMKTHAAERIVDLKSRGFDNAGLYDPQGVGGTHVMYVLHHADTPKIYAGLPDNP
EEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEECCCCEEEECCCCCC
EISPLVGLWKGISKPLGLLAMGAAVLAGFFHYVRIGPNRVEEDEHPDPPDTSVHVVDPAV
CCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCHH
HTFDPRGEGRP
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]