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The map label for this gene is fdoI [H]

Identifier: 77458925

GI number: 77458925

Start: 3110923

End: 3111576

Strand: Direct

Name: fdoI [H]

Synonym: Pfl01_2700

Alternate gene names: 77458925

Gene position: 3110923-3111576 (Clockwise)

Preceding gene: 77458924

Following gene: 77458926

Centisome position: 48.32

GC content: 59.33

Gene sequence:

>654_bases
ATGAGCAACAAGACGATCCTGCGCTACACCGCCAACCAGCGCACCAATCACTGGCTGGTGGCGATTCTGTTCTTCATGGC
CGGGCTGTCGGGGCTGGCGCTGTTTCATCCGTCGATGTTCTGGCTGACAAACCTGTTCGGCGGCGGGCCGTGGACGCGAA
TCCTGCACCCGTTCATGGGCGTGCTGATGTTCGTGTTCTTCCTCGGCCTGGTGTTTCGCTTCTGGCGCTCGAACTTCTTC
ATCGACAACGACTGGAAATGGCTGCGGCGCATCGACCGGGTGATGGTCAACGATGAAGAAACCGTGCCGGCGGTGGGCAA
GTACAACGCCGGGCAAAAGCTGCTGTTCTGGACTTTACTGCTGTGCATGCTCGGATTGCTGTTCACGGGACTGGTGATCT
GGCGCGCGTATTTCAGTCATTACTTCGGCATCACCACCATCCGCTGGGCGATGTTGCTGCACGCACTGGCGGGGTTTGTG
CTGATCCTGAGCATCATCGTTCACATCTACGCCGGAATCTGGATCAAGGGTTCGGTGGACGCGATGATGCACGGCTGGGT
CAGCCGCGCCTGGGCCAGGAAACACCATGCACTCTGGTATCGCGAGGTGGAAAGCCAGGATCCGCCGGAGCGACCGGTCA
CGAAAAAGGGCTGA

Upstream 100 bases:

>100_bases
ACCGGGTCGAGGAAGATGAACATCCCGATCCGCCCGACACCTCGGTGCACGTCGTCGATCCGGCAGTGCACACCTTCGAC
CCACGCGGGGAGGGCCGGCC

Downstream 100 bases:

>100_bases
CAGTTGCCAACCATCCTCGAACCCGGAGAAATCGAAGCGGCGGCCAGTTCGCCGCCGTTTCTGCATTTGCCGCCACCCAA
CCTGTTCATGCTGCGAGCCC

Product: formate dehydrogenase subunit gamma

Products: CO2; NADH

Alternate protein names: Aerobic formate dehydrogenase cytochrome b556 subunit; FDH-Z subunit gamma; Formate dehydrogenase-O subunit gamma [H]

Number of amino acids: Translated: 217; Mature: 216

Protein sequence:

>217_residues
MSNKTILRYTANQRTNHWLVAILFFMAGLSGLALFHPSMFWLTNLFGGGPWTRILHPFMGVLMFVFFLGLVFRFWRSNFF
IDNDWKWLRRIDRVMVNDEETVPAVGKYNAGQKLLFWTLLLCMLGLLFTGLVIWRAYFSHYFGITTIRWAMLLHALAGFV
LILSIIVHIYAGIWIKGSVDAMMHGWVSRAWARKHHALWYREVESQDPPERPVTKKG

Sequences:

>Translated_217_residues
MSNKTILRYTANQRTNHWLVAILFFMAGLSGLALFHPSMFWLTNLFGGGPWTRILHPFMGVLMFVFFLGLVFRFWRSNFF
IDNDWKWLRRIDRVMVNDEETVPAVGKYNAGQKLLFWTLLLCMLGLLFTGLVIWRAYFSHYFGITTIRWAMLLHALAGFV
LILSIIVHIYAGIWIKGSVDAMMHGWVSRAWARKHHALWYREVESQDPPERPVTKKG
>Mature_216_residues
SNKTILRYTANQRTNHWLVAILFFMAGLSGLALFHPSMFWLTNLFGGGPWTRILHPFMGVLMFVFFLGLVFRFWRSNFFI
DNDWKWLRRIDRVMVNDEETVPAVGKYNAGQKLLFWTLLLCMLGLLFTGLVIWRAYFSHYFGITTIRWAMLLHALAGFVL
ILSIIVHIYAGIWIKGSVDAMMHGWVSRAWARKHHALWYREVESQDPPERPVTKKG

Specific function: Allows to use formate as major electron donor during aerobic respiration. Subunit gamma is probably the cytochrome b556(FDO) component of the formate dehydrogenase [H]

COG id: COG2864

COG function: function code C; Cytochrome b subunit of formate dehydrogenase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the formate dehydrogenase gamma subunit family [H]

Homologues:

Organism=Escherichia coli, GI1790325, Length=208, Percent_Identity=45.1923076923077, Blast_Score=201, Evalue=4e-53,
Organism=Escherichia coli, GI1787750, Length=210, Percent_Identity=43.3333333333333, Blast_Score=183, Evalue=6e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005797
- InterPro:   IPR016174
- InterPro:   IPR006471 [H]

Pfam domain/function: PF00033 Cytochrom_B_N [H]

EC number: 1.2.1.2

Molecular weight: Translated: 25413; Mature: 25282

Theoretical pI: Translated: 10.76; Mature: 10.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNKTILRYTANQRTNHWLVAILFFMAGLSGLALFHPSMFWLTNLFGGGPWTRILHPFMG
CCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
VLMFVFFLGLVFRFWRSNFFIDNDWKWLRRIDRVMVNDEETVPAVGKYNAGQKLLFWTLL
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHH
LCMLGLLFTGLVIWRAYFSHYFGITTIRWAMLLHALAGFVLILSIIVHIYAGIWIKGSVD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
AMMHGWVSRAWARKHHALWYREVESQDPPERPVTKKG
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
SNKTILRYTANQRTNHWLVAILFFMAGLSGLALFHPSMFWLTNLFGGGPWTRILHPFMG
CCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
VLMFVFFLGLVFRFWRSNFFIDNDWKWLRRIDRVMVNDEETVPAVGKYNAGQKLLFWTLL
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHH
LCMLGLLFTGLVIWRAYFSHYFGITTIRWAMLLHALAGFVLILSIIVHIYAGIWIKGSVD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
AMMHGWVSRAWARKHHALWYREVESQDPPERPVTKKG
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]