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The map label for this gene is gcvA [H]

Identifier: 77458668

GI number: 77458668

Start: 2795287

End: 2796189

Strand: Direct

Name: gcvA [H]

Synonym: Pfl01_2443

Alternate gene names: 77458668

Gene position: 2795287-2796189 (Clockwise)

Preceding gene: 77458664

Following gene: 77458669

Centisome position: 43.42

GC content: 60.8

Gene sequence:

>903_bases
ATGCTGCCCCGATTGCCATCGCTTAACGGATTGCGCGCCTTCGAATGCGCCGCCCGGCATATGAGCTTCACCCGCGCCGC
CGAAGAACTGAACGTCACGCAGACCGCGATCAGCCATCAGATCCGTCGCCTCGAGGATGAGCTTGGCGTGCGTCTGTTCA
TGCGCTTGAAGGACGGTTTGGCGTTGACCGAGGAGGGCAATGCTTACTTGCCCGGCATTCGTTCGGCGTTTCTTGAATTG
CGCTACTCGACTGAAAAGCTGCTGGAATCCAGCCACAACAGCGTCCTGACCATCAGCACGCTGGTGTCGGTGGCATCGAA
ATGGTTGTTGCCGCGATTGCCATCGTTTCGCGAGGCGCATCCGGACATTGATGTGCGGATCAGTGCTTCCACCGAGTGGG
TGGACTTTCGCAAGGGCGGCATCGATGCGGCGATCCGCTACGGCGACGGGAATTGGCCTGGACTTCGGGCCGACTGGTTG
ATGGCCGACGAGATCTTTCCGGTATGCAGCCCGCGCTTGCTGACCGGCGACAAGCCGTTGAATACGCCGGCGGACCTGGC
CCATCACCCTTTGCTGCAAGTCAGCGGCGTGACCGCCAATGACTGGAACGACTGGCTGCATGCGGCAGGCCAGCCACCGC
TGACGGCCAAAGGCCCACGATTGACCTTCGACCTGGCGATGATGGCGGTGCAGGCGGCGATTGACGGGCAGGGCGTGTGC
ATCGGTCGCTCGACCTATGTCGATGACGATCTGCGCGCAGGCAGGCTGGTGGCGCCGTTTGATTTGCGCTTGAAATCCGC
TTCCGGCTTCTATCTCGTCACGCCCCACGATCAGGCCGAATCGAAAAAGATCGTGGCGTTCAGAGGCTGGCTATCGCAGG
TGCTGGCCAAACCCTTTGTATAA

Upstream 100 bases:

>100_bases
CTTGCAGATGGAGCCTCAGAATCCGCTATCGCCGCGCCGCTTCGCAAAGGACAAATGCAGTGCTAGCGTATGACAAAATC
TCATGGACTGAAGAGGTCAT

Downstream 100 bases:

>100_bases
GCGTGTATCCATCGCGCCGCCGTACACAAACGGGTAACAAACCCCGTCATCAGGACACATCCTGCGTACATGCCTCGGCC
AAAGTAGTCGTCAACACATC

Product: DNA-binding transcriptional activator GcvA

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MLPRLPSLNGLRAFECAARHMSFTRAAEELNVTQTAISHQIRRLEDELGVRLFMRLKDGLALTEEGNAYLPGIRSAFLEL
RYSTEKLLESSHNSVLTISTLVSVASKWLLPRLPSFREAHPDIDVRISASTEWVDFRKGGIDAAIRYGDGNWPGLRADWL
MADEIFPVCSPRLLTGDKPLNTPADLAHHPLLQVSGVTANDWNDWLHAAGQPPLTAKGPRLTFDLAMMAVQAAIDGQGVC
IGRSTYVDDDLRAGRLVAPFDLRLKSASGFYLVTPHDQAESKKIVAFRGWLSQVLAKPFV

Sequences:

>Translated_300_residues
MLPRLPSLNGLRAFECAARHMSFTRAAEELNVTQTAISHQIRRLEDELGVRLFMRLKDGLALTEEGNAYLPGIRSAFLEL
RYSTEKLLESSHNSVLTISTLVSVASKWLLPRLPSFREAHPDIDVRISASTEWVDFRKGGIDAAIRYGDGNWPGLRADWL
MADEIFPVCSPRLLTGDKPLNTPADLAHHPLLQVSGVTANDWNDWLHAAGQPPLTAKGPRLTFDLAMMAVQAAIDGQGVC
IGRSTYVDDDLRAGRLVAPFDLRLKSASGFYLVTPHDQAESKKIVAFRGWLSQVLAKPFV
>Mature_300_residues
MLPRLPSLNGLRAFECAARHMSFTRAAEELNVTQTAISHQIRRLEDELGVRLFMRLKDGLALTEEGNAYLPGIRSAFLEL
RYSTEKLLESSHNSVLTISTLVSVASKWLLPRLPSFREAHPDIDVRISASTEWVDFRKGGIDAAIRYGDGNWPGLRADWL
MADEIFPVCSPRLLTGDKPLNTPADLAHHPLLQVSGVTANDWNDWLHAAGQPPLTAKGPRLTFDLAMMAVQAAIDGQGVC
IGRSTYVDDDLRAGRLVAPFDLRLKSASGFYLVTPHDQAESKKIVAFRGWLSQVLAKPFV

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=292, Percent_Identity=49.6575342465753, Blast_Score=265, Evalue=4e-72,
Organism=Escherichia coli, GI1786448, Length=289, Percent_Identity=37.3702422145329, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI1788706, Length=294, Percent_Identity=35.7142857142857, Blast_Score=155, Evalue=4e-39,
Organism=Escherichia coli, GI1786401, Length=276, Percent_Identity=28.2608695652174, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1787589, Length=274, Percent_Identity=26.6423357664234, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI145693193, Length=303, Percent_Identity=25.7425742574257, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1787128, Length=142, Percent_Identity=30.9859154929577, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI87081978, Length=257, Percent_Identity=26.0700389105058, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1789639, Length=251, Percent_Identity=25.4980079681275, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI145693105, Length=123, Percent_Identity=30.8943089430894, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33196; Mature: 33196

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: PS50931 HTH_LYSR ; PS00290 IG_MHC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPRLPSLNGLRAFECAARHMSFTRAAEELNVTQTAISHQIRRLEDELGVRLFMRLKDGL
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEHHCCE
ALTEEGNAYLPGIRSAFLELRYSTEKLLESSHNSVLTISTLVSVASKWLLPRLPSFREAH
EEECCCCEECCHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHCCCCCHHHCC
PDIDVRISASTEWVDFRKGGIDAAIRYGDGNWPGLRADWLMADEIFPVCSPRLLTGDKPL
CCEEEEEECCCCEEHHHCCCCEEEEEECCCCCCCCCCCEEEHHHHCCCCCCEEECCCCCC
NTPADLAHHPLLQVSGVTANDWNDWLHAAGQPPLTAKGPRLTFDLAMMAVQAAIDGQGVC
CCCHHHHHCCCEEECCCCCCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEE
IGRSTYVDDDLRAGRLVAPFDLRLKSASGFYLVTPHDQAESKKIVAFRGWLSQVLAKPFV
EECCCCCCCCCCCCCEECCCEEEEECCCCEEEECCCCCCCCCEEEEHHHHHHHHHHCCCC
>Mature Secondary Structure
MLPRLPSLNGLRAFECAARHMSFTRAAEELNVTQTAISHQIRRLEDELGVRLFMRLKDGL
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEHHCCE
ALTEEGNAYLPGIRSAFLELRYSTEKLLESSHNSVLTISTLVSVASKWLLPRLPSFREAH
EEECCCCEECCHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHCCCCCHHHCC
PDIDVRISASTEWVDFRKGGIDAAIRYGDGNWPGLRADWLMADEIFPVCSPRLLTGDKPL
CCEEEEEECCCCEEHHHCCCCEEEEEECCCCCCCCCCCEEEHHHHCCCCCCEEECCCCCC
NTPADLAHHPLLQVSGVTANDWNDWLHAAGQPPLTAKGPRLTFDLAMMAVQAAIDGQGVC
CCCHHHHHCCCEEECCCCCCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEE
IGRSTYVDDDLRAGRLVAPFDLRLKSASGFYLVTPHDQAESKKIVAFRGWLSQVLAKPFV
EECCCCCCCCCCCCCEECCCEEEEECCCCEEEECCCCCCCCCEEEEHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]