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The map label for this gene is fosA [H]

Identifier: 77457548

GI number: 77457548

Start: 1495726

End: 1496142

Strand: Direct

Name: fosA [H]

Synonym: Pfl01_1321

Alternate gene names: 77457548

Gene position: 1495726-1496142 (Clockwise)

Preceding gene: 77457547

Following gene: 77457549

Centisome position: 23.23

GC content: 66.19

Gene sequence:

>417_bases
ATGCTCACCGGCCTCAACCACCTGACCCTCGCCGTCACCGACCTGGACAGCAGCCTCGCGTTCTACCGCGACGTGCTGAA
GCTGCGAGTCGAGGCCACCTGGGACGCCGGTGCCTACCTGTCGCTCCCGGGCCTGTGGTTGTGCCTGTCCCTCGATCCGA
AGCGCCATCCCGAGCCCGCCGCCGACTACACCCACTACGCCTTCAGCATTGGCGCGGCGGATTTCCCGCTGTTCGTGCAA
CTGCTTCGCGCCGCCAACGTGCAGGAATGGCGCGACAACCGCAGCGAAGGCGCGTCGTTCTACTTCCTCGACCCGGACGG
TCACAAGCTCGAAGCCCACGTCGGCGATCTGGCCTCGCGACTGGCCGCGTGTCGGCAAAAGCCCTACGCGGGCATGGGTT
TTTTCAACGAGCCGTGA

Upstream 100 bases:

>100_bases
TGCGTTTCCAAAACCACCGACCTGAAGAAAACCCAAATCACCCTGACCTACGACGGCAAGCAATACCTCATCCCCGAAGA
CCTCAGAGGTTACTGACCCG

Downstream 100 bases:

>100_bases
TCATCCGTTAATCGCTGATATAGACTGGCGGCCACGTTTTCCGGGACTTGCAGGTTTTCCATGACTCCATCGTTGCTAAT
GGCCGTGCTGGCCTCCGGTT

Product: glyoxalase/bleomycin resistance protein/dioxygenase

Products: NA

Alternate protein names: Fosfomycin resistance protein [H]

Number of amino acids: Translated: 138; Mature: 138

Protein sequence:

>138_residues
MLTGLNHLTLAVTDLDSSLAFYRDVLKLRVEATWDAGAYLSLPGLWLCLSLDPKRHPEPAADYTHYAFSIGAADFPLFVQ
LLRAANVQEWRDNRSEGASFYFLDPDGHKLEAHVGDLASRLAACRQKPYAGMGFFNEP

Sequences:

>Translated_138_residues
MLTGLNHLTLAVTDLDSSLAFYRDVLKLRVEATWDAGAYLSLPGLWLCLSLDPKRHPEPAADYTHYAFSIGAADFPLFVQ
LLRAANVQEWRDNRSEGASFYFLDPDGHKLEAHVGDLASRLAACRQKPYAGMGFFNEP
>Mature_138_residues
MLTGLNHLTLAVTDLDSSLAFYRDVLKLRVEATWDAGAYLSLPGLWLCLSLDPKRHPEPAADYTHYAFSIGAADFPLFVQ
LLRAANVQEWRDNRSEGASFYFLDPDGHKLEAHVGDLASRLAACRQKPYAGMGFFNEP

Specific function: Metalloglutathione transferase which confers resistance to fosfomycin by catalyzing the addition of glutathione to fosfomycin [H]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fosfomycin resistance protein family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =2.5.1.18 [H]

Molecular weight: Translated: 15388; Mature: 15388

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTGLNHLTLAVTDLDSSLAFYRDVLKLRVEATWDAGAYLSLPGLWLCLSLDPKRHPEPA
CCCCCCEEEEEEECCCHHHHHHHHHHHHHEEECCCCCCEEECCCEEEEEECCCCCCCCCC
ADYTHYAFSIGAADFPLFVQLLRAANVQEWRDNRSEGASFYFLDPDGHKLEAHVGDLASR
CCCCEEEEEECCCCHHHHHHHHHHCCHHHHHCCCCCCCEEEEECCCCCEEHHHHHHHHHH
LAACRQKPYAGMGFFNEP
HHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MLTGLNHLTLAVTDLDSSLAFYRDVLKLRVEATWDAGAYLSLPGLWLCLSLDPKRHPEPA
CCCCCCEEEEEEECCCHHHHHHHHHHHHHEEECCCCCCEEECCCEEEEEECCCCCCCCCC
ADYTHYAFSIGAADFPLFVQLLRAANVQEWRDNRSEGASFYFLDPDGHKLEAHVGDLASR
CCCCEEEEEECCCCHHHHHHHHHHCCHHHHHCCCCCCCEEEEECCCCCEEHHHHHHHHHH
LAACRQKPYAGMGFFNEP
HHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]