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The map label for this gene is kgtP [H]

Identifier: 77457499

GI number: 77457499

Start: 1445006

End: 1446340

Strand: Direct

Name: kgtP [H]

Synonym: Pfl01_1272

Alternate gene names: 77457499

Gene position: 1445006-1446340 (Clockwise)

Preceding gene: 77457498

Following gene: 77457500

Centisome position: 22.44

GC content: 60.45

Gene sequence:

>1335_bases
ATGTCTAGACTGTCACTGTCCCTATCGAGTGAAAAAATAATGACAACCACCACCTCCCATTACACCGGCGAAGAGCGCAG
CAAGCGCATCTTTGCCATTGTCGGTGCCTCGTCCGGCAACCTCGTCGAATGGTTCGACTTCTACGTCTACGCTTTCTGCG
CGATCTACTTCGCCCCGGCGTTTTTTCCCTCGGACAACCCCACGGTGCAGCTGGTCAACACCGCTGGCGTTTTCGCCGCC
GGGTTTCTGATGCGACCGATCGGCGGCTGGATTTTCGGCCGGGTCGCAGACAAGCACGGGCGCAAGAACTCGATGCTGAT
CTCGATCCTGATGATGTGCTTCGGCTCGTTGTTGATCGCCTGTCTGCCGACCTACAAGGACATCGGCGTCTGGGCGCCGC
TGTTGCTGTTGTTCGCGCGTCTGCTGCAAGGCCTGTCGGTGGGCGGTGAGTACGGCACCACCGCGACCTACATGAGCGAA
GTCGCGCTCAAGGGCCAGCGCGGGTTCTTCGCCTCGTTCCAGTACGTGACCCTGATCGGCGGGCAACTGCTGGCGGTGCT
GCTGGTGGTGATCCTGCAACAGTTCCTCACCGAAGAAGAATTGCGTGCCTACGGCTGGCGGATTCCGTTCGTGGTCGGTG
CGGCCGCCGCGCTGATTTCGCTGTTCCTGCGTCGCTCGCTGAAAGAAACCACGAGCAAGGAAATGCGCGAGAACAAGGAC
GCCGGCAGCATCCGCGCACTGTTCCGCGACCACAAGGCTGCGTTCATCACCGTGCTGGGTTACACCGCCGGCGGCTCGCT
GATTTTCTACACCTTCACCACGTACATGCAGAAGTACCTGGTGAACACCGCCGGCATGCACGCCAAGACCGCCAGCTACA
TCATGACCGGCGCGCTGTTCCTGTACATGTGCATGCAGCCGCTGTTCGGCATGCTCGCCGACAAGATCGGCCGGCGTAAC
TCGATGCTCTGGTTCGGCGGCCTCGGCACGTTGTTCACAGTGCCGATCCTGCTCAGCCTGAAAAGTGTCGGCAGTCCGTT
CCTGGCCTTTGTGCTGATCACCCTGGCGCTGGCGATCGTCAGTTTCTACACCTCGATCAGCGGTCTGGTGAAAGCCGAGA
TGTTCCCGCCGGAAGTCCGCGCCCTCGGCGTCGGCCTGGCCTACGCGGTGGCTAACGCGATCTTCGGCGGGTCGGCCGAA
TACGTGGCCCTGAGCCTCAAGGCTGGCGGCATGGAAAACGCGTTCTACTGGTATGTCACGATCATGATGGCCGTGGCATT
CCTGTTCAGCCTGCGCTTGCCCAAAGAAGCGAAGTATTTGCACCACGACCTTTAA

Upstream 100 bases:

>100_bases
ATTATTGAAAGAACACCGATCGCCACGCTCCGCGCTGATCGTTCCCACGCGGAGCGTGGGAACGATCAATACAGGATGTG
GAACGGGACTGTTGCGAAGT

Downstream 100 bases:

>100_bases
ACCGATGCGCGCGGGCCACACCGGCCCGCGCCGGCAAGGACTGTTTATGACTCAGCGACCGGGCAATCAATTGTTCGATG
CCTACTTTACTGCCCGCGAT

Product: citrate-proton symport

Products: Proton [Cytoplasm]; 2-oxoglutarate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 444; Mature: 443

Protein sequence:

>444_residues
MSRLSLSLSSEKIMTTTTSHYTGEERSKRIFAIVGASSGNLVEWFDFYVYAFCAIYFAPAFFPSDNPTVQLVNTAGVFAA
GFLMRPIGGWIFGRVADKHGRKNSMLISILMMCFGSLLIACLPTYKDIGVWAPLLLLFARLLQGLSVGGEYGTTATYMSE
VALKGQRGFFASFQYVTLIGGQLLAVLLVVILQQFLTEEELRAYGWRIPFVVGAAAALISLFLRRSLKETTSKEMRENKD
AGSIRALFRDHKAAFITVLGYTAGGSLIFYTFTTYMQKYLVNTAGMHAKTASYIMTGALFLYMCMQPLFGMLADKIGRRN
SMLWFGGLGTLFTVPILLSLKSVGSPFLAFVLITLALAIVSFYTSISGLVKAEMFPPEVRALGVGLAYAVANAIFGGSAE
YVALSLKAGGMENAFYWYVTIMMAVAFLFSLRLPKEAKYLHHDL

Sequences:

>Translated_444_residues
MSRLSLSLSSEKIMTTTTSHYTGEERSKRIFAIVGASSGNLVEWFDFYVYAFCAIYFAPAFFPSDNPTVQLVNTAGVFAA
GFLMRPIGGWIFGRVADKHGRKNSMLISILMMCFGSLLIACLPTYKDIGVWAPLLLLFARLLQGLSVGGEYGTTATYMSE
VALKGQRGFFASFQYVTLIGGQLLAVLLVVILQQFLTEEELRAYGWRIPFVVGAAAALISLFLRRSLKETTSKEMRENKD
AGSIRALFRDHKAAFITVLGYTAGGSLIFYTFTTYMQKYLVNTAGMHAKTASYIMTGALFLYMCMQPLFGMLADKIGRRN
SMLWFGGLGTLFTVPILLSLKSVGSPFLAFVLITLALAIVSFYTSISGLVKAEMFPPEVRALGVGLAYAVANAIFGGSAE
YVALSLKAGGMENAFYWYVTIMMAVAFLFSLRLPKEAKYLHHDL
>Mature_443_residues
SRLSLSLSSEKIMTTTTSHYTGEERSKRIFAIVGASSGNLVEWFDFYVYAFCAIYFAPAFFPSDNPTVQLVNTAGVFAAG
FLMRPIGGWIFGRVADKHGRKNSMLISILMMCFGSLLIACLPTYKDIGVWAPLLLLFARLLQGLSVGGEYGTTATYMSEV
ALKGQRGFFASFQYVTLIGGQLLAVLLVVILQQFLTEEELRAYGWRIPFVVGAAAALISLFLRRSLKETTSKEMRENKDA
GSIRALFRDHKAAFITVLGYTAGGSLIFYTFTTYMQKYLVNTAGMHAKTASYIMTGALFLYMCMQPLFGMLADKIGRRNS
MLWFGGLGTLFTVPILLSLKSVGSPFLAFVLITLALAIVSFYTSISGLVKAEMFPPEVRALGVGLAYAVANAIFGGSAEY
VALSLKAGGMENAFYWYVTIMMAVAFLFSLRLPKEAKYLHHDL

Specific function: Uptake of alpha-ketoglutarate across the boundary membrane with the concomitant import of a cation (symport system) [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788942, Length=423, Percent_Identity=69.5035460992908, Blast_Score=571, Evalue=1e-164,
Organism=Escherichia coli, GI1790550, Length=403, Percent_Identity=30.272952853598, Blast_Score=202, Evalue=4e-53,
Organism=Escherichia coli, GI1788292, Length=409, Percent_Identity=31.0513447432763, Blast_Score=184, Evalue=8e-48,
Organism=Escherichia coli, GI1789941, Length=440, Percent_Identity=31.5909090909091, Blast_Score=161, Evalue=6e-41,
Organism=Escherichia coli, GI87082404, Length=317, Percent_Identity=28.7066246056782, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI87082231, Length=332, Percent_Identity=25.3012048192771, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 48819; Mature: 48688

Theoretical pI: Translated: 9.70; Mature: 9.70

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLSLSLSSEKIMTTTTSHYTGEERSKRIFAIVGASSGNLVEWFDFYVYAFCAIYFAPA
CCCEEEECCCCEEEEEECCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHC
FFPSDNPTVQLVNTAGVFAAGFLMRPIGGWIFGRVADKHGRKNSMLISILMMCFGSLLIA
CCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
CLPTYKDIGVWAPLLLLFARLLQGLSVGGEYGTTATYMSEVALKGQRGFFASFQYVTLIG
HHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHH
GQLLAVLLVVILQQFLTEEELRAYGWRIPFVVGAAAALISLFLRRSLKETTSKEMRENKD
HHHHHHHHHHHHHHHHCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
AGSIRALFRDHKAAFITVLGYTAGGSLIFYTFTTYMQKYLVNTAGMHAKTASYIMTGALF
CHHHHHHHHHCCCEEEEEEEECCCCCEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
LYMCMQPLFGMLADKIGRRNSMLWFGGLGTLFTVPILLSLKSVGSPFLAFVLITLALAIV
HHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
SFYTSISGLVKAEMFPPEVRALGVGLAYAVANAIFGGSAEYVALSLKAGGMENAFYWYVT
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHH
IMMAVAFLFSLRLPKEAKYLHHDL
HHHHHHHHHHHCCCHHHHHHHCCC
>Mature Secondary Structure 
SRLSLSLSSEKIMTTTTSHYTGEERSKRIFAIVGASSGNLVEWFDFYVYAFCAIYFAPA
CCEEEECCCCEEEEEECCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHC
FFPSDNPTVQLVNTAGVFAAGFLMRPIGGWIFGRVADKHGRKNSMLISILMMCFGSLLIA
CCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
CLPTYKDIGVWAPLLLLFARLLQGLSVGGEYGTTATYMSEVALKGQRGFFASFQYVTLIG
HHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHH
GQLLAVLLVVILQQFLTEEELRAYGWRIPFVVGAAAALISLFLRRSLKETTSKEMRENKD
HHHHHHHHHHHHHHHHCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
AGSIRALFRDHKAAFITVLGYTAGGSLIFYTFTTYMQKYLVNTAGMHAKTASYIMTGALF
CHHHHHHHHHCCCEEEEEEEECCCCCEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
LYMCMQPLFGMLADKIGRRNSMLWFGGLGTLFTVPILLSLKSVGSPFLAFVLITLALAIV
HHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
SFYTSISGLVKAEMFPPEVRALGVGLAYAVANAIFGGSAEYVALSLKAGGMENAFYWYVT
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHH
IMMAVAFLFSLRLPKEAKYLHHDL
HHHHHHHHHHHCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; 2-oxoglutarate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + 2-oxoglutarate [Periplasm] = Proton [Cytoplasm] + 2-oxoglutarate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2118499; 9205837; 9278503; 1709493; 2190187; 2053984; 8419306 [H]