Click here to switch to the map view.

The map label for this gene is pcaB [H]

Identifier: 77457500

GI number: 77457500

Start: 1446387

End: 1447751

Strand: Direct

Name: pcaB [H]

Synonym: Pfl01_1273

Alternate gene names: 77457500

Gene position: 1446387-1447751 (Clockwise)

Preceding gene: 77457499

Following gene: 77457501

Centisome position: 22.46

GC content: 65.93

Gene sequence:

>1365_bases
ATGACTCAGCGACCGGGCAATCAATTGTTCGATGCCTACTTTACTGCCCGCGATATGCGCGACGTGTTCTGCGATCAGGG
CCGGGTGCAGGCGATGCTCGACTTCGAAGCGGCGCTGGCCCGGGCCGAGGCGCGGGTCGGCTTGATTCCGGCGGATGCTG
TCGCGGCCATCGAAAACGCCTGCCGCGCCGGGCTGTTTGATTTCGCAGCGCTGGGCGAGGCGATTGCCACGGCCGGCAAT
TCGGCGATTCCACTGGTCAAGGCATTGGGCAAACAGATTGCCGCGACCGATGCCGAAGCCGAGCGCTATGTGCATCTGGG
CGCCACCAGCCAGGACGTGATGGATTCCGGGTTGGTGCTGCAATTGCGTCAGGCGCTGGAATTGATTGAAAGGGAGTTGG
GGCAACTGGCCGACGCCCTCGCCACTCAGGCGCAGCGCCATGCCGCGACGCCGCTGGCAGGACGTACCTGGCTGCAACAT
GCGACGCCGGTGACGCTCGGCATGAAGATCGCCGGTTGGCTCGGTGCGATCACGCGCAGCCGTCAGCGTCTGCGCGAGCT
CAAACCGCGACTGCTGGTGCTGCAATTCGGCGGCGCGTCCGGCACCCTCGCGGCGCTCGGCGAACAGGCGTTGCCGATCG
CCCAGGCGCTGGCCGAAGAACTGCACCTGAACCTGCCGGAACAACCGTGGCACACCCAGCGCGATCGCATCGTCGAGTTC
GGCGCAGTACTCGGGCTGATCGCCGGCAGCCTCGGCAAGTTCGGCCGCGACATCAGCTTGTTGATGCAGACCGAAGCGGC
GGAAGTGTTCGAGCCGTCGGCGCCGGGCAAGGGCGGTTCCTCGACCATGCCGCACAAGCGCAATCCGGTGGGCGCGGCGG
TGTTGATCGGTGCCGCGACCCGGGTGCCGGGGCTGGTCTCGACGCTGTTCAGCGCCATGCCGCAGGAACACGAGCGCAGC
CTTGGTCTGTGGCACGCCGAATGGGAAACCCTGCCGGAAATCTGCTGCCTGGTGTCGGGCGCACTGCAACAGGCGCGGCT
GCTGGCCGATGGTCTGGAAGTCGATGCCGCGCGCATGGCCCGCAACCTGGAGTTGACTCAAGGCCTGGTGCTGGCCGAAG
CGGTGAGCATCGTGCTCGCGCAACGGGTCGGTCGTGATACCGCGCATCATCTGCTCGAGCAATGCTGCAAACGAGCGGTG
GCCGAACAGCGTCACCTGCGTGCGGTTCTCGGTGACGAGCCGCAGGTGACCGCCGAACTGAGCGCAAGCGAACTGGATCA
TCTGCTGGATCCCGCCCACTACCTCGGTCAGGCGCAGGTCTGGGTCGAGCGGGCAGTAGCCGAACACAACGCATTGTCTG
ACTGA

Upstream 100 bases:

>100_bases
CTGTTCAGCCTGCGCTTGCCCAAAGAAGCGAAGTATTTGCACCACGACCTTTAAACCGATGCGCGCGGGCCACACCGGCC
CGCGCCGGCAAGGACTGTTT

Downstream 100 bases:

>100_bases
AAGGAGAGGGCTGTGGCTTTCGTTCAACTCGCCGATGGCGAACTGCATTACTCATTGGAAAGCCCGGTCGATGCGCCGGT
GCTGGTGCTGTCCAACTCGC

Product: 3-carboxy-cis,cis-muconate cycloisomerase

Products: NA

Alternate protein names: 3-carboxymuconate lactonizing enzyme; CMLE [H]

Number of amino acids: Translated: 454; Mature: 453

Protein sequence:

>454_residues
MTQRPGNQLFDAYFTARDMRDVFCDQGRVQAMLDFEAALARAEARVGLIPADAVAAIENACRAGLFDFAALGEAIATAGN
SAIPLVKALGKQIAATDAEAERYVHLGATSQDVMDSGLVLQLRQALELIERELGQLADALATQAQRHAATPLAGRTWLQH
ATPVTLGMKIAGWLGAITRSRQRLRELKPRLLVLQFGGASGTLAALGEQALPIAQALAEELHLNLPEQPWHTQRDRIVEF
GAVLGLIAGSLGKFGRDISLLMQTEAAEVFEPSAPGKGGSSTMPHKRNPVGAAVLIGAATRVPGLVSTLFSAMPQEHERS
LGLWHAEWETLPEICCLVSGALQQARLLADGLEVDAARMARNLELTQGLVLAEAVSIVLAQRVGRDTAHHLLEQCCKRAV
AEQRHLRAVLGDEPQVTAELSASELDHLLDPAHYLGQAQVWVERAVAEHNALSD

Sequences:

>Translated_454_residues
MTQRPGNQLFDAYFTARDMRDVFCDQGRVQAMLDFEAALARAEARVGLIPADAVAAIENACRAGLFDFAALGEAIATAGN
SAIPLVKALGKQIAATDAEAERYVHLGATSQDVMDSGLVLQLRQALELIERELGQLADALATQAQRHAATPLAGRTWLQH
ATPVTLGMKIAGWLGAITRSRQRLRELKPRLLVLQFGGASGTLAALGEQALPIAQALAEELHLNLPEQPWHTQRDRIVEF
GAVLGLIAGSLGKFGRDISLLMQTEAAEVFEPSAPGKGGSSTMPHKRNPVGAAVLIGAATRVPGLVSTLFSAMPQEHERS
LGLWHAEWETLPEICCLVSGALQQARLLADGLEVDAARMARNLELTQGLVLAEAVSIVLAQRVGRDTAHHLLEQCCKRAV
AEQRHLRAVLGDEPQVTAELSASELDHLLDPAHYLGQAQVWVERAVAEHNALSD
>Mature_453_residues
TQRPGNQLFDAYFTARDMRDVFCDQGRVQAMLDFEAALARAEARVGLIPADAVAAIENACRAGLFDFAALGEAIATAGNS
AIPLVKALGKQIAATDAEAERYVHLGATSQDVMDSGLVLQLRQALELIERELGQLADALATQAQRHAATPLAGRTWLQHA
TPVTLGMKIAGWLGAITRSRQRLRELKPRLLVLQFGGASGTLAALGEQALPIAQALAEELHLNLPEQPWHTQRDRIVEFG
AVLGLIAGSLGKFGRDISLLMQTEAAEVFEPSAPGKGGSSTMPHKRNPVGAAVLIGAATRVPGLVSTLFSAMPQEHERSL
GLWHAEWETLPEICCLVSGALQQARLLADGLEVDAARMARNLELTQGLVLAEAVSIVLAQRVGRDTAHHLLEQCCKRAVA
EQRHLRAVLGDEPQVTAELSASELDHLLDPAHYLGQAQVWVERAVAEHNALSD

Specific function: Catalyzes an anti cycloisomerization [H]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=456, Percent_Identity=26.0964912280702, Blast_Score=90, Evalue=5e-18,
Organism=Homo sapiens, GI183227688, Length=397, Percent_Identity=24.6851385390428, Blast_Score=73, Evalue=6e-13,
Organism=Escherichia coli, GI1790398, Length=386, Percent_Identity=24.3523316062176, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1787896, Length=352, Percent_Identity=25.5681818181818, Blast_Score=62, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17508577, Length=377, Percent_Identity=23.0769230769231, Blast_Score=68, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI32564234, Length=265, Percent_Identity=24.5283018867925, Blast_Score=65, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6324993, Length=377, Percent_Identity=25.7294429708223, Blast_Score=72, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6323391, Length=369, Percent_Identity=24.390243902439, Blast_Score=64, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24647570, Length=460, Percent_Identity=25.4347826086956, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI78710009, Length=277, Percent_Identity=27.7978339350181, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24640179, Length=326, Percent_Identity=27.6073619631902, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24640177, Length=338, Percent_Identity=26.9230769230769, Blast_Score=66, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR012789 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =5.5.1.2 [H]

Molecular weight: Translated: 48702; Mature: 48571

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQRPGNQLFDAYFTARDMRDVFCDQGRVQAMLDFEAALARAEARVGLIPADAVAAIENA
CCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
CRAGLFDFAALGEAIATAGNSAIPLVKALGKQIAATDAEAERYVHLGATSQDVMDSGLVL
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHEEECCCCHHHHHHCHHHH
QLRQALELIERELGQLADALATQAQRHAATPLAGRTWLQHATPVTLGMKIAGWLGAITRS
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHH
RQRLRELKPRLLVLQFGGASGTLAALGEQALPIAQALAEELHLNLPEQPWHTQRDRIVEF
HHHHHHCCCEEEEEEECCCCCHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
GAVLGLIAGSLGKFGRDISLLMQTEAAEVFEPSAPGKGGSSTMPHKRNPVGAAVLIGAAT
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
RVPGLVSTLFSAMPQEHERSLGLWHAEWETLPEICCLVSGALQQARLLADGLEVDAARMA
HCCHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
RNLELTQGLVLAEAVSIVLAQRVGRDTAHHLLEQCCKRAVAEQRHLRAVLGDEPQVTAEL
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHC
SASELDHLLDPAHYLGQAQVWVERAVAEHNALSD
CHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TQRPGNQLFDAYFTARDMRDVFCDQGRVQAMLDFEAALARAEARVGLIPADAVAAIENA
CCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
CRAGLFDFAALGEAIATAGNSAIPLVKALGKQIAATDAEAERYVHLGATSQDVMDSGLVL
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHEEECCCCHHHHHHCHHHH
QLRQALELIERELGQLADALATQAQRHAATPLAGRTWLQHATPVTLGMKIAGWLGAITRS
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHH
RQRLRELKPRLLVLQFGGASGTLAALGEQALPIAQALAEELHLNLPEQPWHTQRDRIVEF
HHHHHHCCCEEEEEEECCCCCHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
GAVLGLIAGSLGKFGRDISLLMQTEAAEVFEPSAPGKGGSSTMPHKRNPVGAAVLIGAAT
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
RVPGLVSTLFSAMPQEHERSLGLWHAEWETLPEICCLVSGALQQARLLADGLEVDAARMA
HCCHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
RNLELTQGLVLAEAVSIVLAQRVGRDTAHHLLEQCCKRAVAEQRHLRAVLGDEPQVTAEL
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHC
SASELDHLLDPAHYLGQAQVWVERAVAEHNALSD
CHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1390752 [H]