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The map label for this gene is oprF [H]

Identifier: 77457447

GI number: 77457447

Start: 1391627

End: 1392322

Strand: Direct

Name: oprF [H]

Synonym: Pfl01_1220

Alternate gene names: 77457447

Gene position: 1391627-1392322 (Clockwise)

Preceding gene: 77457442

Following gene: 77457448

Centisome position: 21.61

GC content: 60.78

Gene sequence:

>696_bases
ATGAGCATAGTTCGGACAGCATTACCCTTGGTTCTGCTAACCAGTGTGTTGACTGGTTGCGCAGGTTTGCAGAAAACCGA
CTGGCCGACCTGTGCGGCAGTCGGTGGTGTCGTCGGTGCGGGTCTCGGCGCGACCGAAAGCTCCGCATGGGCGGGGTATG
GCGCGTTACTCGTCGGCGGTACGGCAGCAGCCTATTGCTGGGTTCACGGTGATGGCGACGAAGACGGCGATGGTGTGCCG
GACAGTCGCGACAAGTGCCCGGGCACGCCTAAAGGCGTACAGGTCGATGCTGACGGCTGCCCTCCACCAGCCCCTGCACC
AGTGGTCGAAGAAGCTGTAGTGGTCAAGGAAGAAACCATTGTCATCCGCGATGTTCACTTCCAGTTCGACAAGGCCACGT
TGACCCCGGCCGACAAACTGGTCCTGGATAAGGTCGCTTCGCGCCTGAAACAGGAATCCAGCACGGCGCAACTGACCGTG
ACCGGCCACACCGACAGCGTCGGCAGCGATGCCTACAACCAGAAACTGTCGGATCGCCGCGCGCACTCGGTGGTCAAGTA
CCTGGTTGAAAGCGGCGTACCGCAAAGCAGCTTCGTGTCGGTGACCGGTGCCGGTGAAAGCCAGCCGGTGGCAGACAACA
AAACTGCCGACGGTCGCGCGCTGAACCGTCGTACCGAAATCAAAATCAACCGCTAG

Upstream 100 bases:

>100_bases
ACAGGCGCACAAACAGCAATGGCCTATTGCTGGCGGAGATTAAAACTCCGGTCTTTACTCCCTGAATCGGTTCTAAGCCG
ATCCCTCAGGAGAAAACTAG

Downstream 100 bases:

>100_bases
CCCCCTCGCATCCGCGGCTTGTGCAGTCGCGGATGCGGGTCTTTACTCCTGTGTAACCGGTATGGGCCGGTGACACAGGA
GCTTTAACGATGAGTGTTCT

Product: OmpA/MotB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 231; Mature: 230

Protein sequence:

>231_residues
MSIVRTALPLVLLTSVLTGCAGLQKTDWPTCAAVGGVVGAGLGATESSAWAGYGALLVGGTAAAYCWVHGDGDEDGDGVP
DSRDKCPGTPKGVQVDADGCPPPAPAPVVEEAVVVKEETIVIRDVHFQFDKATLTPADKLVLDKVASRLKQESSTAQLTV
TGHTDSVGSDAYNQKLSDRRAHSVVKYLVESGVPQSSFVSVTGAGESQPVADNKTADGRALNRRTEIKINR

Sequences:

>Translated_231_residues
MSIVRTALPLVLLTSVLTGCAGLQKTDWPTCAAVGGVVGAGLGATESSAWAGYGALLVGGTAAAYCWVHGDGDEDGDGVP
DSRDKCPGTPKGVQVDADGCPPPAPAPVVEEAVVVKEETIVIRDVHFQFDKATLTPADKLVLDKVASRLKQESSTAQLTV
TGHTDSVGSDAYNQKLSDRRAHSVVKYLVESGVPQSSFVSVTGAGESQPVADNKTADGRALNRRTEIKINR
>Mature_230_residues
SIVRTALPLVLLTSVLTGCAGLQKTDWPTCAAVGGVVGAGLGATESSAWAGYGALLVGGTAAAYCWVHGDGDEDGDGVPD
SRDKCPGTPKGVQVDADGCPPPAPAPVVEEAVVVKEETIVIRDVHFQFDKATLTPADKLVLDKVASRLKQESSTAQLTVT
GHTDSVGSDAYNQKLSDRRAHSVVKYLVESGVPQSSFVSVTGAGESQPVADNKTADGRALNRRTEIKINR

Specific function: Has porin activity, forming small water-filled channels. Also has a structural role in determining cell shape and ability to grow in low-osmolarity medium [H]

COG id: COG2885

COG function: function code M; Outer membrane protein and related peptidoglycan-associated (lipo)proteins

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 OmpA-like domain [H]

Homologues:

Organism=Escherichia coli, GI1787191, Length=116, Percent_Identity=43.1034482758621, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI48994946, Length=107, Percent_Identity=42.0560747663551, Blast_Score=72, Evalue=3e-14,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1480 Molecules/Cell In: Growth Phase, Mi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011250
- InterPro:   IPR006664
- InterPro:   IPR006690
- InterPro:   IPR006665
- InterPro:   IPR008722 [H]

Pfam domain/function: PF00691 OmpA; PF05736 OprF [H]

EC number: NA

Molecular weight: Translated: 23955; Mature: 23824

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS51123 OMPA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIVRTALPLVLLTSVLTGCAGLQKTDWPTCAAVGGVVGAGLGATESSAWAGYGALLVGG
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEECC
TAAAYCWVHGDGDEDGDGVPDSRDKCPGTPKGVQVDADGCPPPAPAPVVEEAVVVKEETI
CEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCHHHHHEEEECCEE
VIRDVHFQFDKATLTPADKLVLDKVASRLKQESSTAQLTVTGHTDSVGSDAYNQKLSDRR
EEEEEEEEECCEECCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHH
AHSVVKYLVESGVPQSSFVSVTGAGESQPVADNKTADGRALNRRTEIKINR
HHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEECC
>Mature Secondary Structure 
SIVRTALPLVLLTSVLTGCAGLQKTDWPTCAAVGGVVGAGLGATESSAWAGYGALLVGG
CHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEECC
TAAAYCWVHGDGDEDGDGVPDSRDKCPGTPKGVQVDADGCPPPAPAPVVEEAVVVKEETI
CEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCHHHHHEEEECCEE
VIRDVHFQFDKATLTPADKLVLDKVASRLKQESSTAQLTVTGHTDSVGSDAYNQKLSDRR
EEEEEEEEECCEECCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHH
AHSVVKYLVESGVPQSSFVSVTGAGESQPVADNKTADGRALNRRTEIKINR
HHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2447060; 10984043 [H]