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The map label for this gene is oprF [H]

Identifier: 77457448

GI number: 77457448

Start: 1392412

End: 1393134

Strand: Direct

Name: oprF [H]

Synonym: Pfl01_1221

Alternate gene names: 77457448

Gene position: 1392412-1393134 (Clockwise)

Preceding gene: 77457447

Following gene: 77457449

Centisome position: 21.63

GC content: 63.07

Gene sequence:

>723_bases
ATGAGTGTTCTTACAAGGTCCGTCTTGCCGGTTCTGCTGCTGGGCAGCCTGTTGACCGGTTGCGCCACCCACAGCGATGG
CACCGCTCCCCTCAATCAACGTACCTGGCCGATCTGCAGCCTCATTGGCGGACTGGTCGGCGGCGGTCTCGGCGCGCTCG
AAAGTGGCGGCTGGGCCGGCGGTGGCGCTGCGCTGGGGATCCTCACCGGCGGATTGATCTGCTATGCCCAGGATGGCGAT
GAAGACGACGACGGCGTGTTCGATCGCCGCGACCGTTGCCCCGATACCCCGGCCAACACCCCGGTCGACCACCGTGGCTG
TCCGCTGCCGCAATATCCGGTCACCGAGAAACCTGCCGAACCGGCCCCGCAAACCGAAGTCATCACCCTCAACGATGCCG
GCAACGTGCTGTTCGACTTCGACAAGTCCGATCTGAAACCGGCCGCGAAAAGCCAGCTGGACACGCTGATGGACAAACTG
CGCAATGCCGACGTGGTGAGCATCAAGGTCATCGGTCACACCGACAGCAAGGGTTCGGACGCCTACAACCAGGCACTTTC
GGAGCGCCGCGCCAGCAGTGTGGCGGAGTATTTGCTGAGTCAGGGCCTGGCGCCGAACAAACTCACCAGTGAAGGGCGTG
GCGAGAGCGAACCGGTCGCCGATAACAACACGGACGAGGGGCGTGCACAAAACCGTCGGGTGGAACTGCACATCAATCGT
TAG

Upstream 100 bases:

>100_bases
TCAACCGCTAGCCCCCTCGCATCCGCGGCTTGTGCAGTCGCGGATGCGGGTCTTTACTCCTGTGTAACCGGTATGGGCCG
GTGACACAGGAGCTTTAACG

Downstream 100 bases:

>100_bases
GATGCGTCTGTAGTCCGCAGGCTAGAGCTGTCGGTCGATGATCCTTGTCGAACCGGCAGCCATCCGGCGGTGGATCGGTT
TTTTTCATTTTTCCCTCTGG

Product: OmpA/MotB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 240; Mature: 239

Protein sequence:

>240_residues
MSVLTRSVLPVLLLGSLLTGCATHSDGTAPLNQRTWPICSLIGGLVGGGLGALESGGWAGGGAALGILTGGLICYAQDGD
EDDDGVFDRRDRCPDTPANTPVDHRGCPLPQYPVTEKPAEPAPQTEVITLNDAGNVLFDFDKSDLKPAAKSQLDTLMDKL
RNADVVSIKVIGHTDSKGSDAYNQALSERRASSVAEYLLSQGLAPNKLTSEGRGESEPVADNNTDEGRAQNRRVELHINR

Sequences:

>Translated_240_residues
MSVLTRSVLPVLLLGSLLTGCATHSDGTAPLNQRTWPICSLIGGLVGGGLGALESGGWAGGGAALGILTGGLICYAQDGD
EDDDGVFDRRDRCPDTPANTPVDHRGCPLPQYPVTEKPAEPAPQTEVITLNDAGNVLFDFDKSDLKPAAKSQLDTLMDKL
RNADVVSIKVIGHTDSKGSDAYNQALSERRASSVAEYLLSQGLAPNKLTSEGRGESEPVADNNTDEGRAQNRRVELHINR
>Mature_239_residues
SVLTRSVLPVLLLGSLLTGCATHSDGTAPLNQRTWPICSLIGGLVGGGLGALESGGWAGGGAALGILTGGLICYAQDGDE
DDDGVFDRRDRCPDTPANTPVDHRGCPLPQYPVTEKPAEPAPQTEVITLNDAGNVLFDFDKSDLKPAAKSQLDTLMDKLR
NADVVSIKVIGHTDSKGSDAYNQALSERRASSVAEYLLSQGLAPNKLTSEGRGESEPVADNNTDEGRAQNRRVELHINR

Specific function: Has porin activity, forming small water-filled channels. Also has a structural role in determining cell shape and ability to grow in low-osmolarity medium [H]

COG id: COG2885

COG function: function code M; Outer membrane protein and related peptidoglycan-associated (lipo)proteins

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 OmpA-like domain [H]

Homologues:

Organism=Escherichia coli, GI1787191, Length=126, Percent_Identity=44.4444444444444, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI48994946, Length=112, Percent_Identity=41.0714285714286, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1788957, Length=110, Percent_Identity=39.0909090909091, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1786962, Length=101, Percent_Identity=31.6831683168317, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1480 Molecules/Cell In: Growth Phase, Mi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011250
- InterPro:   IPR006664
- InterPro:   IPR006690
- InterPro:   IPR006665
- InterPro:   IPR008722 [H]

Pfam domain/function: PF00691 OmpA; PF05736 OprF [H]

EC number: NA

Molecular weight: Translated: 25317; Mature: 25186

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01068 OMPA_1 ; PS51123 OMPA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVLTRSVLPVLLLGSLLTGCATHSDGTAPLNQRTWPICSLIGGLVGGGLGALESGGWAG
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHCCCCCCC
GGAALGILTGGLICYAQDGDEDDDGVFDRRDRCPDTPANTPVDHRGCPLPQYPVTEKPAE
CHHHHHHHHCCEEEEECCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PAPQTEVITLNDAGNVLFDFDKSDLKPAAKSQLDTLMDKLRNADVVSIKVIGHTDSKGSD
CCCCCEEEEEECCCCEEEECCHHHCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCH
AYNQALSERRASSVAEYLLSQGLAPNKLTSEGRGESEPVADNNTDEGRAQNRRVELHINR
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECC
>Mature Secondary Structure 
SVLTRSVLPVLLLGSLLTGCATHSDGTAPLNQRTWPICSLIGGLVGGGLGALESGGWAG
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHCCCCCCC
GGAALGILTGGLICYAQDGDEDDDGVFDRRDRCPDTPANTPVDHRGCPLPQYPVTEKPAE
CHHHHHHHHCCEEEEECCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PAPQTEVITLNDAGNVLFDFDKSDLKPAAKSQLDTLMDKLRNADVVSIKVIGHTDSKGSD
CCCCCEEEEEECCCCEEEECCHHHCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCH
AYNQALSERRASSVAEYLLSQGLAPNKLTSEGRGESEPVADNNTDEGRAQNRRVELHINR
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1898935 [H]