Click here to switch to the map view.

The map label for this gene is prpB [C]

Identifier: 77457301

GI number: 77457301

Start: 1242762

End: 1243598

Strand: Direct

Name: prpB [C]

Synonym: Pfl01_1074

Alternate gene names: 77457301

Gene position: 1242762-1243598 (Clockwise)

Preceding gene: 77457300

Following gene: 77457302

Centisome position: 19.3

GC content: 64.28

Gene sequence:

>837_bases
ATGGACGCTCAAGCCCGTCAACAACTAGCCCGTAAAGCCGAAGCCTTCAAAGCCCTGCACGAACGCCCCGGGATTTTCGT
GATTCCCAATCCATGGGATGCCGGTTCCGCGAAGATGCTCGCCAGTCTCGGCTATCAGGCGCTGGCGACCACCAGCGCCG
GCTATGCGTTTTCCCAGGGCAATCCCGATGGCGGCCTGAGCCTCGACGATACCCTGGCCAATGTCCGGGCGATTGTCGCG
GCCACCGATCTGCCGGTGGCGGTGGATCTGGAAAACGGTTTCGCCGACGCCCCGGCCGAATCGGCGCAGAGTCTGATCCG
CGCCGCCGAGGCGGGCGCTGTGGGCGGCTCGATCGAAGATGCCACCGGCCGGGAAGACTCACCGATCTACTGTTTCGAAC
ACGCTGTGGCGCGGATCGAAACCGCCGTCGCTGCCGTGCGCACCTTGCCGTTTCCCTTCACCTTGACGGCCCGTGCGGAA
AACTACCTGCACGGCAATCCCGATCTCAACGACACCCTCCGCCGTCTGCAGGCCTTTGCCGAAGCCGGCGCCGACGTGCT
GTACGCGCCGGGCCTGCGCACCGCCGAAGAAGTTCTGGCGGTGGTGCGCGCGGTGGCGCCGAAACCGGTCAATGTGTTGA
TGTCCGGCGGCTTGAAGCTGACGGTGCAGCAGCTCGAGGAAATGGGCGTGAAGCGGATCAGCACCGGTTCGGCGCTGGCC
CTGGCGGCGTTCGGCGAGTTTTTCCGCGCGGCTGAAGAAATCCAGCAATCCGGCACGTTCACCTTCACTTCCCGCTCGAT
GCCGTACGCCAAGGCCAATCAATTCTTCAAGGGATGA

Upstream 100 bases:

>100_bases
TGCCTGGCCTGCCGTCCCTGCCTGAGCTATACAGAGAACCCGCCGCCGAGCAACTTGGCCTGCTCTGAGGCGGATTTCTT
TCCTGCCCAGGAGCCTGCCG

Downstream 100 bases:

>100_bases
GCATGGGGCGCTGGCTACTGTTGGCGGGGCTGCTGATCATCGGTGGCGGCCTGATCGGCGTCTGGCGCGGCTGGCTCGAT
GTGCCGCCGCGGTGGAATCC

Product: carboxyvinyl-carboxyphosphonate phosphorylmutase

Products: Pyruvate; Succinate [C]

Alternate protein names: NA

Number of amino acids: Translated: 278; Mature: 278

Protein sequence:

>278_residues
MDAQARQQLARKAEAFKALHERPGIFVIPNPWDAGSAKMLASLGYQALATTSAGYAFSQGNPDGGLSLDDTLANVRAIVA
ATDLPVAVDLENGFADAPAESAQSLIRAAEAGAVGGSIEDATGREDSPIYCFEHAVARIETAVAAVRTLPFPFTLTARAE
NYLHGNPDLNDTLRRLQAFAEAGADVLYAPGLRTAEEVLAVVRAVAPKPVNVLMSGGLKLTVQQLEEMGVKRISTGSALA
LAAFGEFFRAAEEIQQSGTFTFTSRSMPYAKANQFFKG

Sequences:

>Translated_278_residues
MDAQARQQLARKAEAFKALHERPGIFVIPNPWDAGSAKMLASLGYQALATTSAGYAFSQGNPDGGLSLDDTLANVRAIVA
ATDLPVAVDLENGFADAPAESAQSLIRAAEAGAVGGSIEDATGREDSPIYCFEHAVARIETAVAAVRTLPFPFTLTARAE
NYLHGNPDLNDTLRRLQAFAEAGADVLYAPGLRTAEEVLAVVRAVAPKPVNVLMSGGLKLTVQQLEEMGVKRISTGSALA
LAAFGEFFRAAEEIQQSGTFTFTSRSMPYAKANQFFKG
>Mature_278_residues
MDAQARQQLARKAEAFKALHERPGIFVIPNPWDAGSAKMLASLGYQALATTSAGYAFSQGNPDGGLSLDDTLANVRAIVA
ATDLPVAVDLENGFADAPAESAQSLIRAAEAGAVGGSIEDATGREDSPIYCFEHAVARIETAVAAVRTLPFPFTLTARAE
NYLHGNPDLNDTLRRLQAFAEAGADVLYAPGLRTAEEVLAVVRAVAPKPVNVLMSGGLKLTVQQLEEMGVKRISTGSALA
LAAFGEFFRAAEEIQQSGTFTFTSRSMPYAKANQFFKG

Specific function: Catalyzes The Formation Of Pyruvate And Succinate From 2-Methylisocitrate (By Similarity). [C]

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786525, Length=286, Percent_Identity=30.4195804195804, Blast_Score=72, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR015813 [H]

Pfam domain/function: NA

EC number: 4.1.3.30 [C]

Molecular weight: Translated: 29379; Mature: 29379

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDAQARQQLARKAEAFKALHERPGIFVIPNPWDAGSAKMLASLGYQALATTSAGYAFSQG
CCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHCHHHHHHCCCCCEECCC
NPDGGLSLDDTLANVRAIVAATDLPVAVDLENGFADAPAESAQSLIRAAEAGAVGGSIED
CCCCCCCHHHHHHHHHHHEEECCCCEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCC
ATGREDSPIYCFEHAVARIETAVAAVRTLPFPFTLTARAENYLHGNPDLNDTLRRLQAFA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHH
EAGADVLYAPGLRTAEEVLAVVRAVAPKPVNVLMSGGLKLTVQQLEEMGVKRISTGSALA
HCCCCEEECCCCCCHHHHHHHHHHHCCCCHHHHHCCCCEEHHHHHHHCCHHEECCCCHHH
LAAFGEFFRAAEEIQQSGTFTFTSRSMPYAKANQFFKG
HHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHCCC
>Mature Secondary Structure
MDAQARQQLARKAEAFKALHERPGIFVIPNPWDAGSAKMLASLGYQALATTSAGYAFSQG
CCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHCHHHHHHCCCCCEECCC
NPDGGLSLDDTLANVRAIVAATDLPVAVDLENGFADAPAESAQSLIRAAEAGAVGGSIED
CCCCCCCHHHHHHHHHHHEEECCCCEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCC
ATGREDSPIYCFEHAVARIETAVAAVRTLPFPFTLTARAENYLHGNPDLNDTLRRLQAFA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHH
EAGADVLYAPGLRTAEEVLAVVRAVAPKPVNVLMSGGLKLTVQQLEEMGVKRISTGSALA
HCCCCEEECCCCCCHHHHHHHHHHHCCCCHHHHHCCCCEEHHHHHHHCCHHEECCCCHHH
LAAFGEFFRAAEEIQQSGTFTFTSRSMPYAKANQFFKG
HHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: methylisocitrate [C]

Specific reaction: methylisocitrate <==> Pyruvate + Succinate [C]

General reaction: Elimination [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]