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The map label for this gene is lysS [H]

Identifier: 77457287

GI number: 77457287

Start: 1228949

End: 1230448

Strand: Direct

Name: lysS [H]

Synonym: Pfl01_1060

Alternate gene names: 77457287

Gene position: 1228949-1230448 (Clockwise)

Preceding gene: 77457286

Following gene: 77457288

Centisome position: 19.09

GC content: 60.8

Gene sequence:

>1500_bases
ATGAGCGACCAACAACTCGACCAGGCCCTGCAACAGGAAGAAAACTCCCTGATCGCCCTGCGCAAGGAAAAGCTTGCTGC
CGAGCGCGCCAAGGGCAATGCCTTCCCGAACGACTTCCGCCGCGACAACTACTGCGATGCGCTGCAGAAACAGTACGCGG
ACAAGACCAAGGAAGAGCTGGCAGAGGCGGCGATCCCGGTCAAGGTTGCAGGTCGCATCATGCTCAACCGTGGCTCGTTC
ATGGTGATCCAGGACATGACCGGTCGCATCCAGGTCTACGTCAACCGCAAGACCCTGTCCGAAGAAACCCTGGCCGCGGT
GAAAACCTGGGACATGGGCGACATCATCGCCGCCGAAGGCACCCTGGCCCGTTCCGGCAAGGGCGACCTGTACGTCGAGA
TGACCAGCGTGCGCCTGCTGACCAAATCCCTGCGCCCGCTGCCGGACAAGCACCACGGCCTGACCGACACCGAACAGCGC
TACCGTCAGCGCTACGTTGACCTGATCGTCAACGAAGACGTGCGTCAGACTTTCCGCGTGCGTTCGCAAGTGATCGCGCA
CATCCGCAGCTTCCTGATGAAGCGTGACTTCCTCGAAGTCGAAACGCCGATGCTGCAGACCATTCCTGGCGGCGCCGCGG
CCAAACCGTTCGAAACCCACCACAACGCGCTGGACATGGAAATGTTCCTGCGCATCGCGCCAGAGCTGTACCTCAAGCGC
CTGGTGGTTGGCGGTTTCGAGAAAGTATTCGAGATCAACCGCAACTTCCGTAACGAAGGTGTTTCGACGCGTCACAACCC
TGAGTTCACCATGTTGGAGTTCTACCAGGCTTACGCCGACTACGAAGACAACATGGACCTGACCGAAGAACTGTTCCGTG
AACTGGCGCAGTTGGTTCTGGGCAGCACCGACGTGCCGTACGGCGACAAGGTGTTCCACTTCGGCGAGCCGTTCGTGCGT
CTGTCGGTGTTCGACTCGATCCTCAAGTACAACCCTGAGCTGACCGCCGATGACCTGAACGACATCGACAAGGCGCGCGC
CATCGCCAAGAAGGCCGGCGCCAAGGTGCTGGGCTTCGAAGGTCTGGGCAAGCTGCAGGTGATGATTTTCGAAGAGCTGG
TCGAGCACAAGCTGGAACAGCCGCACTTCATTACCCAGTATCCGTTCGAAGTGTCGCCGCTGGCCCGTCGCAACGACGAC
AACCCGAACGTCACCGACCGCTTCGAGCTGTTCATCGGCGGCCGTGAAATCGCCAACGCCTACTCCGAGCTGAACGACGC
GGAAGACCAGGCCGAGCGCTTCATGGCCCAGGTGGCCGACAAGGACGCCGGCGACGACGAAGCCATGCACTACGACGCCG
ACTTCGTTCGTGCGCTGGAGTACGGCATGCCGCCAACGGCCGGTGAAGGCATCGGCATTGACCGTCTGGTGATGCTGCTG
ACCAACTCCCCGTCGATCCGCGACGTGATCCTGTTCCCGCACATGCGGCCACAAGCGTAA

Upstream 100 bases:

>100_bases
ATCGGCTGATTCAACTCGATCCCTGTAGGAGCCAGCCTGCTGGCGATCCTCGGGCCGAAAGGCCCGGGGGCAACGAACCT
GTGATGGAATATTTAAAGAC

Downstream 100 bases:

>100_bases
GTGTTTCAATAAAAAAGCCGCCTGAAATGGGCGGCTTTTTATTGCCTGTCTGGTACAAACGTATCAAATGGTTAATTTTG
AAGAAAACAACAGAGGTATG

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS [H]

Number of amino acids: Translated: 499; Mature: 498

Protein sequence:

>499_residues
MSDQQLDQALQQEENSLIALRKEKLAAERAKGNAFPNDFRRDNYCDALQKQYADKTKEELAEAAIPVKVAGRIMLNRGSF
MVIQDMTGRIQVYVNRKTLSEETLAAVKTWDMGDIIAAEGTLARSGKGDLYVEMTSVRLLTKSLRPLPDKHHGLTDTEQR
YRQRYVDLIVNEDVRQTFRVRSQVIAHIRSFLMKRDFLEVETPMLQTIPGGAAAKPFETHHNALDMEMFLRIAPELYLKR
LVVGGFEKVFEINRNFRNEGVSTRHNPEFTMLEFYQAYADYEDNMDLTEELFRELAQLVLGSTDVPYGDKVFHFGEPFVR
LSVFDSILKYNPELTADDLNDIDKARAIAKKAGAKVLGFEGLGKLQVMIFEELVEHKLEQPHFITQYPFEVSPLARRNDD
NPNVTDRFELFIGGREIANAYSELNDAEDQAERFMAQVADKDAGDDEAMHYDADFVRALEYGMPPTAGEGIGIDRLVMLL
TNSPSIRDVILFPHMRPQA

Sequences:

>Translated_499_residues
MSDQQLDQALQQEENSLIALRKEKLAAERAKGNAFPNDFRRDNYCDALQKQYADKTKEELAEAAIPVKVAGRIMLNRGSF
MVIQDMTGRIQVYVNRKTLSEETLAAVKTWDMGDIIAAEGTLARSGKGDLYVEMTSVRLLTKSLRPLPDKHHGLTDTEQR
YRQRYVDLIVNEDVRQTFRVRSQVIAHIRSFLMKRDFLEVETPMLQTIPGGAAAKPFETHHNALDMEMFLRIAPELYLKR
LVVGGFEKVFEINRNFRNEGVSTRHNPEFTMLEFYQAYADYEDNMDLTEELFRELAQLVLGSTDVPYGDKVFHFGEPFVR
LSVFDSILKYNPELTADDLNDIDKARAIAKKAGAKVLGFEGLGKLQVMIFEELVEHKLEQPHFITQYPFEVSPLARRNDD
NPNVTDRFELFIGGREIANAYSELNDAEDQAERFMAQVADKDAGDDEAMHYDADFVRALEYGMPPTAGEGIGIDRLVMLL
TNSPSIRDVILFPHMRPQA
>Mature_498_residues
SDQQLDQALQQEENSLIALRKEKLAAERAKGNAFPNDFRRDNYCDALQKQYADKTKEELAEAAIPVKVAGRIMLNRGSFM
VIQDMTGRIQVYVNRKTLSEETLAAVKTWDMGDIIAAEGTLARSGKGDLYVEMTSVRLLTKSLRPLPDKHHGLTDTEQRY
RQRYVDLIVNEDVRQTFRVRSQVIAHIRSFLMKRDFLEVETPMLQTIPGGAAAKPFETHHNALDMEMFLRIAPELYLKRL
VVGGFEKVFEINRNFRNEGVSTRHNPEFTMLEFYQAYADYEDNMDLTEELFRELAQLVLGSTDVPYGDKVFHFGEPFVRL
SVFDSILKYNPELTADDLNDIDKARAIAKKAGAKVLGFEGLGKLQVMIFEELVEHKLEQPHFITQYPFEVSPLARRNDDN
PNVTDRFELFIGGREIANAYSELNDAEDQAERFMAQVADKDAGDDEAMHYDADFVRALEYGMPPTAGEGIGIDRLVMLLT
NSPSIRDVILFPHMRPQA

Specific function: Also Can Synthesize A Number Of Adenyl Dinucleotides (In Particular Appppa). These Dinucleotides Have Been Proposed To Act As Modulators Of The Heat-Shock Response And Stress Response. [C]

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI194272210, Length=514, Percent_Identity=41.4396887159533, Blast_Score=361, Evalue=1e-99,
Organism=Homo sapiens, GI5031815, Length=514, Percent_Identity=41.4396887159533, Blast_Score=361, Evalue=1e-99,
Organism=Homo sapiens, GI45439306, Length=356, Percent_Identity=23.876404494382, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI40789249, Length=292, Percent_Identity=23.6301369863014, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1790571, Length=483, Percent_Identity=62.5258799171843, Blast_Score=616, Evalue=1e-178,
Organism=Escherichia coli, GI1789256, Length=488, Percent_Identity=61.6803278688525, Blast_Score=615, Evalue=1e-177,
Organism=Escherichia coli, GI87082379, Length=323, Percent_Identity=33.1269349845201, Blast_Score=145, Evalue=8e-36,
Organism=Escherichia coli, GI1788173, Length=231, Percent_Identity=28.5714285714286, Blast_Score=69, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17535925, Length=500, Percent_Identity=41.4, Blast_Score=348, Evalue=3e-96,
Organism=Caenorhabditis elegans, GI71994340, Length=500, Percent_Identity=41.4, Blast_Score=348, Evalue=4e-96,
Organism=Caenorhabditis elegans, GI17535927, Length=500, Percent_Identity=41.4, Blast_Score=348, Evalue=4e-96,
Organism=Caenorhabditis elegans, GI17551876, Length=489, Percent_Identity=24.3353783231084, Blast_Score=99, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI32566633, Length=237, Percent_Identity=24.8945147679325, Blast_Score=79, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6320242, Length=496, Percent_Identity=41.5322580645161, Blast_Score=348, Evalue=1e-96,
Organism=Saccharomyces cerevisiae, GI6324256, Length=413, Percent_Identity=35.1089588377724, Blast_Score=190, Evalue=5e-49,
Organism=Saccharomyces cerevisiae, GI6323011, Length=360, Percent_Identity=26.6666666666667, Blast_Score=103, Evalue=5e-23,
Organism=Drosophila melanogaster, GI24640849, Length=488, Percent_Identity=40.9836065573771, Blast_Score=370, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24640851, Length=488, Percent_Identity=40.9836065573771, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI17136276, Length=327, Percent_Identity=24.7706422018349, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24584738, Length=261, Percent_Identity=29.5019157088123, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.6 [H]

Molecular weight: Translated: 56919; Mature: 56788

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDQQLDQALQQEENSLIALRKEKLAAERAKGNAFPNDFRRDNYCDALQKQYADKTKEEL
CCHHHHHHHHHHHCCCEEHHHHHHHHHHHHCCCCCCCHHCCCHHHHHHHHHHHHHHHHHH
AEAAIPVKVAGRIMLNRGSFMVIQDMTGRIQVYVNRKTLSEETLAAVKTWDMGDIIAAEG
HHHCCCHHHHCEEEEECCCEEEEEECCCEEEEEEECCCCCHHHHHHHHCCCCCCEEECCC
TLARSGKGDLYVEMTSVRLLTKSLRPLPDKHHGLTDTEQRYRQRYVDLIVNEDVRQTFRV
CCCCCCCCCEEEEEHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHH
RSQVIAHIRSFLMKRDFLEVETPMLQTIPGGAAAKPFETHHNALDMEMFLRIAPELYLKR
HHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHH
LVVGGFEKVFEINRNFRNEGVSTRHNPEFTMLEFYQAYADYEDNMDLTEELFRELAQLVL
HHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
GSTDVPYGDKVFHFGEPFVRLSVFDSILKYNPELTADDLNDIDKARAIAKKAGAKVLGFE
CCCCCCCCCCEEECCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCEEEECC
GLGKLQVMIFEELVEHKLEQPHFITQYPFEVSPLARRNDDNPNVTDRFELFIGGREIANA
CCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHCCCCCCCCCCEEEEEECCHHHHHH
YSELNDAEDQAERFMAQVADKDAGDDEAMHYDADFVRALEYGMPPTAGEGIGIDRLVMLL
HHHHCCHHHHHHHHHHHHHCCCCCCCCCEECCHHHHHHHHHCCCCCCCCCCCHHHHHHHH
TNSPSIRDVILFPHMRPQA
CCCCCCCEEEEECCCCCCC
>Mature Secondary Structure 
SDQQLDQALQQEENSLIALRKEKLAAERAKGNAFPNDFRRDNYCDALQKQYADKTKEEL
CHHHHHHHHHHHCCCEEHHHHHHHHHHHHCCCCCCCHHCCCHHHHHHHHHHHHHHHHHH
AEAAIPVKVAGRIMLNRGSFMVIQDMTGRIQVYVNRKTLSEETLAAVKTWDMGDIIAAEG
HHHCCCHHHHCEEEEECCCEEEEEECCCEEEEEEECCCCCHHHHHHHHCCCCCCEEECCC
TLARSGKGDLYVEMTSVRLLTKSLRPLPDKHHGLTDTEQRYRQRYVDLIVNEDVRQTFRV
CCCCCCCCCEEEEEHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHH
RSQVIAHIRSFLMKRDFLEVETPMLQTIPGGAAAKPFETHHNALDMEMFLRIAPELYLKR
HHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHH
LVVGGFEKVFEINRNFRNEGVSTRHNPEFTMLEFYQAYADYEDNMDLTEELFRELAQLVL
HHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
GSTDVPYGDKVFHFGEPFVRLSVFDSILKYNPELTADDLNDIDKARAIAKKAGAKVLGFE
CCCCCCCCCCEEECCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCEEEECC
GLGKLQVMIFEELVEHKLEQPHFITQYPFEVSPLARRNDDNPNVTDRFELFIGGREIANA
CCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHCCCCCCCCCCEEEEEECCHHHHHH
YSELNDAEDQAERFMAQVADKDAGDDEAMHYDADFVRALEYGMPPTAGEGIGIDRLVMLL
HHHHCCHHHHHHHHHHHHHCCCCCCCCCEECCHHHHHHHHHCCCCCCCCCCCHHHHHHHH
TNSPSIRDVILFPHMRPQA
CCCCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA