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The map label for this gene is prfB [H]

Identifier: 77457286

GI number: 77457286

Start: 1227991

End: 1228833

Strand: Direct

Name: prfB [H]

Synonym: Pfl01_1059

Alternate gene names: 77457286

Gene position: 1227991-1228833 (Clockwise)

Preceding gene: 77457285

Following gene: 77457287

Centisome position: 19.07

GC content: 60.62

Gene sequence:

>843_bases
ATGGCTGTTGAAGAGAACGACGAGGGCGCGGTCAACGACGTCGTCGAAGTCCTTCAAGGTCTCGAAGAGTCCCTGTCCAA
GCTCGAATTCCGCCGTATGTTCAGCCACGAGATGGACCCGAACAACGCCTACCTGGACATCCAGGCCGGTTCCGGCGGCA
CCGAAGCCCAGGACTGGGCCAACATCCTGCTGCGCATGTACCTGCGCTGGGCCGACAAGCGCGGTTTCGACGCAACCATC
ATGGAGCTGTCGGCCGGTGAAGTCGCCGGGATCAAGGGTGCGACCGTGCACATCAAGGGCGAATACGCCTTTGGCTGGCT
GCGTACCGAGATCGGCGTGCACCGTCTGGTGCGCAAGAGCCCGTTCGACTCCGGCAACCGTCGCCACACCTCGTTCTCCG
CGGTTTTTGTCTCGCCCGAGATCGACGACAAGGTGGAAATCGAAATCAACCCGGCAGACCTGCGGATCGACACCTATCGT
TCCTCCGGTGCCGGTGGTCAGCACGTAAACACCACCGACTCGGCCGTACGTATCACCCACGTACCGACCAACACCGTGGT
GAGCTGCCAGAACGAACGTTCCCAGCACGCCAACAAGGACACCGCCATGAAAATGCTGCGGGCCAAGTTGTACGAGCAGG
AAATGCAGAAGCGCAACGCGGCGTCCCAGGCGCTGGAAGACACCAAGTCGGACATCGGCTGGGGTCACCAGATCCGTTCG
TATGTGCTCGATGCGTCGCGGATCAAGGATCTGCGTACCAACATCGAACGCAGCGACTGCGACAAGGTACTCGACGGCGA
CATCGACGAATACCTGGAAGCCAGCCTGAAATCGGGCCTGTAA

Upstream 100 bases:

>100_bases
CGCCCAGGAACTGGGCCGCGAGCGCGCTGCGCTGGCGCAGATCGTCGATACCCTCGACGAACTGAACGGTGGCCTGGCCG
ATTGCCGCGAGCTGCTGGAC

Downstream 100 bases:

>100_bases
GAAACGCAATACAGGATCGGCTGATTCAACTCGATCCCTGTAGGAGCCAGCCTGCTGGCGATCCTCGGGCCGAAAGGCCC
GGGGGCAACGAACCTGTGAT

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 280; Mature: 279

Protein sequence:

>280_residues
MAVEENDEGAVNDVVEVLQGLEESLSKLEFRRMFSHEMDPNNAYLDIQAGSGGTEAQDWANILLRMYLRWADKRGFDATI
MELSAGEVAGIKGATVHIKGEYAFGWLRTEIGVHRLVRKSPFDSGNRRHTSFSAVFVSPEIDDKVEIEINPADLRIDTYR
SSGAGGQHVNTTDSAVRITHVPTNTVVSCQNERSQHANKDTAMKMLRAKLYEQEMQKRNAASQALEDTKSDIGWGHQIRS
YVLDASRIKDLRTNIERSDCDKVLDGDIDEYLEASLKSGL

Sequences:

>Translated_280_residues
MAVEENDEGAVNDVVEVLQGLEESLSKLEFRRMFSHEMDPNNAYLDIQAGSGGTEAQDWANILLRMYLRWADKRGFDATI
MELSAGEVAGIKGATVHIKGEYAFGWLRTEIGVHRLVRKSPFDSGNRRHTSFSAVFVSPEIDDKVEIEINPADLRIDTYR
SSGAGGQHVNTTDSAVRITHVPTNTVVSCQNERSQHANKDTAMKMLRAKLYEQEMQKRNAASQALEDTKSDIGWGHQIRS
YVLDASRIKDLRTNIERSDCDKVLDGDIDEYLEASLKSGL
>Mature_279_residues
AVEENDEGAVNDVVEVLQGLEESLSKLEFRRMFSHEMDPNNAYLDIQAGSGGTEAQDWANILLRMYLRWADKRGFDATIM
ELSAGEVAGIKGATVHIKGEYAFGWLRTEIGVHRLVRKSPFDSGNRRHTSFSAVFVSPEIDDKVEIEINPADLRIDTYRS
SGAGGQHVNTTDSAVRITHVPTNTVVSCQNERSQHANKDTAMKMLRAKLYEQEMQKRNAASQALEDTKSDIGWGHQIRSY
VLDASRIKDLRTNIERSDCDKVLDGDIDEYLEASLKSGL

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=246, Percent_Identity=40.2439024390244, Blast_Score=168, Evalue=6e-42,
Organism=Homo sapiens, GI34577120, Length=251, Percent_Identity=35.0597609561753, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI166795305, Length=150, Percent_Identity=42, Blast_Score=115, Evalue=3e-26,
Organism=Escherichia coli, GI2367172, Length=280, Percent_Identity=69.6428571428571, Blast_Score=417, Evalue=1e-118,
Organism=Escherichia coli, GI1787462, Length=242, Percent_Identity=44.6280991735537, Blast_Score=197, Evalue=9e-52,
Organism=Caenorhabditis elegans, GI17542784, Length=258, Percent_Identity=30.6201550387597, Blast_Score=102, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6321295, Length=275, Percent_Identity=36, Blast_Score=150, Evalue=2e-37,
Organism=Drosophila melanogaster, GI19921226, Length=207, Percent_Identity=39.6135265700483, Blast_Score=151, Evalue=6e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 31322; Mature: 31191

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVEENDEGAVNDVVEVLQGLEESLSKLEFRRMFSHEMDPNNAYLDIQAGSGGTEAQDWA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHH
NILLRMYLRWADKRGFDATIMELSAGEVAGIKGATVHIKGEYAFGWLRTEIGVHRLVRKS
HHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCEEEEECCEEEHHHHHHHHHHHHHHHC
PFDSGNRRHTSFSAVFVSPEIDDKVEIEINPADLRIDTYRSSGAGGQHVNTTDSAVRITH
CCCCCCCCCCEEEEEEECCCCCCEEEEEECCCCEEEEECCCCCCCCCCCCCCCCEEEEEE
VPTNTVVSCQNERSQHANKDTAMKMLRAKLYEQEMQKRNAASQALEDTKSDIGWGHQIRS
CCCCCEEECCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
YVLDASRIKDLRTNIERSDCDKVLDGDIDEYLEASLKSGL
HHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
AVEENDEGAVNDVVEVLQGLEESLSKLEFRRMFSHEMDPNNAYLDIQAGSGGTEAQDWA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHH
NILLRMYLRWADKRGFDATIMELSAGEVAGIKGATVHIKGEYAFGWLRTEIGVHRLVRKS
HHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCEEEEECCEEEHHHHHHHHHHHHHHHC
PFDSGNRRHTSFSAVFVSPEIDDKVEIEINPADLRIDTYRSSGAGGQHVNTTDSAVRITH
CCCCCCCCCCEEEEEEECCCCCCEEEEEECCCCEEEEECCCCCCCCCCCCCCCCEEEEEE
VPTNTVVSCQNERSQHANKDTAMKMLRAKLYEQEMQKRNAASQALEDTKSDIGWGHQIRS
CCCCCEEECCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
YVLDASRIKDLRTNIERSDCDKVLDGDIDEYLEASLKSGL
HHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]