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The map label for this gene is aacA4 [H]

Identifier: 75674327

GI number: 75674327

Start: 148243

End: 148722

Strand: Direct

Name: aacA4 [H]

Synonym: Nwi_0128

Alternate gene names: 75674327

Gene position: 148243-148722 (Clockwise)

Preceding gene: 75674326

Following gene: 75674328

Centisome position: 4.36

GC content: 66.04

Gene sequence:

>480_bases
ATGGCTTACGGCTTTCGCCCGATGACCGCCGCCGACCTGCCGACGATCCGGCGCTGGCTCGACACGCCGCATGTCGCGGA
ATGGTGGGGCGACCCGGCCGAACAGTTCGAACTGGTCAGCGGCGACCTCGATCATCCGGACATGGCGCAGTTCATCGTCA
CCGACGGCGGGCGCGACTTCGCCTATCTTCAGTGCTACCGGCTCAGCGACTGGCATACCGGCCTCGGCGAGCAGCCGGAT
GGAACGCGCGGGCTCGATCAGTTCATCGGCGAAGCCGGCATGATCGGGCGCGGCCATGGCTCCGCGTTCATCCGCTCCTT
TGCCGACAGGCTGCTCATCAAGGGCGTGCCGCGTATCGTGATCGATCCCGATCCGGATAATGCCCGCGCGATTCGCGCCT
ATGAAAAAGCGGGCTTTCATCGCGACCGTCTCGCGGCAACGCCCGACGGCCCCGCGCTTCTGATGATCCGCGACGCATGA

Upstream 100 bases:

>100_bases
CGCGGAAACGATCGTCCGCAGTGCTCTCGATATCGCCGCCGATATCTGTGTTTATACCAATCGAAACCTGACGATCGAAG
CGCTGGCCGCGAGCTGAGCG

Downstream 100 bases:

>100_bases
GTGTCGTCGGTCGGCGCTCCATCATCGTGAACCGCTTGCCGCGGATCGAATCGCAACCTACCTAGGGTGGTCATGACAGA
TATCTCTCCCCGTGAAATCG

Product: GCN5-related N-acetyltransferase

Products: NA

Alternate protein names: AAC(6'); Amikacin resistance protein [H]

Number of amino acids: Translated: 159; Mature: 158

Protein sequence:

>159_residues
MAYGFRPMTAADLPTIRRWLDTPHVAEWWGDPAEQFELVSGDLDHPDMAQFIVTDGGRDFAYLQCYRLSDWHTGLGEQPD
GTRGLDQFIGEAGMIGRGHGSAFIRSFADRLLIKGVPRIVIDPDPDNARAIRAYEKAGFHRDRLAATPDGPALLMIRDA

Sequences:

>Translated_159_residues
MAYGFRPMTAADLPTIRRWLDTPHVAEWWGDPAEQFELVSGDLDHPDMAQFIVTDGGRDFAYLQCYRLSDWHTGLGEQPD
GTRGLDQFIGEAGMIGRGHGSAFIRSFADRLLIKGVPRIVIDPDPDNARAIRAYEKAGFHRDRLAATPDGPALLMIRDA
>Mature_158_residues
AYGFRPMTAADLPTIRRWLDTPHVAEWWGDPAEQFELVSGDLDHPDMAQFIVTDGGRDFAYLQCYRLSDWHTGLGEQPDG
TRGLDQFIGEAGMIGRGHGSAFIRSFADRLLIKGVPRIVIDPDPDNARAIRAYEKAGFHRDRLAATPDGPALLMIRDA

Specific function: Resistance to antibiotics containing the purpurosamine ring including gentamicin, kanamycin, tobramycin, and neomycin [H]

COG id: COG1670

COG function: function code J; Acetyltransferases, including N-acetylases of ribosomal proteins

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 N-acetyltransferase domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000182
- InterPro:   IPR016181
- InterPro:   IPR019432 [H]

Pfam domain/function: PF00583 Acetyltransf_1; PF10331 AlcB [H]

EC number: =2.3.1.82 [H]

Molecular weight: Translated: 17710; Mature: 17579

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYGFRPMTAADLPTIRRWLDTPHVAEWWGDPAEQFELVSGDLDHPDMAQFIVTDGGRDF
CCCCCCCCCHHCCHHHHHHHCCCCHHHHHCCHHHHHEECCCCCCCCCCEEEEEECCCCCE
AYLQCYRLSDWHTGLGEQPDGTRGLDQFIGEAGMIGRGHGSAFIRSFADRLLIKGVPRIV
EEEEEEECCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEE
IDPDPDNARAIRAYEKAGFHRDRLAATPDGPALLMIRDA
ECCCCCCCHHHHHHHHCCCCCHHCCCCCCCCEEEEEECC
>Mature Secondary Structure 
AYGFRPMTAADLPTIRRWLDTPHVAEWWGDPAEQFELVSGDLDHPDMAQFIVTDGGRDF
CCCCCCCCHHCCHHHHHHHCCCCHHHHHCCHHHHHEECCCCCCCCCCEEEEEECCCCCE
AYLQCYRLSDWHTGLGEQPDGTRGLDQFIGEAGMIGRGHGSAFIRSFADRLLIKGVPRIV
EEEEEEECCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEE
IDPDPDNARAIRAYEKAGFHRDRLAATPDGPALLMIRDA
ECCCCCCCHHHHHHHHCCCCCHHCCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2841303; 1963948 [H]