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The map label for this gene is hslU [H]

Identifier: 75674328

GI number: 75674328

Start: 148795

End: 150096

Strand: Direct

Name: hslU [H]

Synonym: Nwi_0129

Alternate gene names: 75674328

Gene position: 148795-150096 (Clockwise)

Preceding gene: 75674327

Following gene: 75674329

Centisome position: 4.37

GC content: 61.75

Gene sequence:

>1302_bases
ATGACAGATATCTCTCCCCGTGAAATCGTTTCTGAACTCGACCGTTTCATTGTCGGTCAGGCCGACGCCAAGCGCGCGGT
CTCCATCGCATTGCGCAATCGCTGGCGACGCCAGCAGTTGACCGGACCGCTGCGCGAGGAGGTGTTGCCGAAGAACATCC
TGATGATCGGCCCGACCGGCGTCGGCAAGACGGAGATCGCGCGACGGCTGGCGAAACTTGCCAACGCTCCCTTTCTCAAG
ATCGAGGCGACCAAGTTCACCGAGGTGGGATATGTCGGACGCGACGTCGAACAGATCGTGCGCGATATCGTCGAGGTCGC
GATCAACCAGACCCGTGAGCGCAAGCGCAAGGACGTTCAGGCGCGCGCGCAGCTTGCCGCGGAGGAGCGCGTTCTGGATG
CTCTGGTCGGCGCCAATGCAAGCGCCGCCACGCGTGATTCATTTCGAAAGAGATTGCGCAGCGGCGAACTCAACGACAAG
GAGATCGAGATCGAGACGCAGACCTCCGGAGGAGGTCCGATGTTCGAGATTCCGGGGATGCCGGGCGCGCAGGTCGGCGC
GATCTCGATCGGCGATATTTTCGGCAAGATGGGCGGGCGAACCAAGACCCGCCGGCTCACCGTCTCGGACTCCTATGAGA
TCCTGATCAACGAGGAATCCGACAAGCTGCTCGATAGCGATCAGCTCACGCAGGAAGCGATCGCCGCGGTCGAGAATAAC
GGCATCGTGTTCCTCGACGAGATCGACAAGATCTGCGTGCGTGACGGGCGCAGCGGCGGCGATGTGTCGCGCGAGGGCGT
GCAGCGCGACCTGCTGCCGCTGATTGAAGGAACCACCGTCTCGACCAAGCACGGCGCCGTTAAGACCGACCATATCCTGT
TCATCGCATCAGGCGCGTTTCACATCGCCAAGCCCTCCGATCTGCTGCCTGAATTGCAGGGCCGGCTGCCGATCCGCGTC
GAACTGGACGCTTTGACGCGCGATGACCTGCGGCGCATCCTGACCGAGCCGGAGGCCTCGCTCATCAAACAGTACGTCGC
ATTGATGGACACCGAGGGAGTCGTGCTCGACTTCACCGATGATGCCGTCGATGCGCTTGCCGATATCGCGGTCGCGGTGA
ATTCGACGGTGGAGAACATCGGCGCGAGGCGGTTGCAGACCGTGATGGAGCGGGTGCTCGACGACATCTCGTTCACGGCC
CCGGATCGCAATGGCGAGACGGTCCGCATCGACGCCGGATATGTCCAGAAAAATATCGGCGACCTGGCCAAGAATGCCGA
CCTGAGCAGGTTCATCCTTTAG

Upstream 100 bases:

>100_bases
CGCGCTTCTGATGATCCGCGACGCATGAGTGTCGTCGGTCGGCGCTCCATCATCGTGAACCGCTTGCCGCGGATCGAATC
GCAACCTACCTAGGGTGGTC

Downstream 100 bases:

>100_bases
ACCACGATGGTTCTGGATCGTATCGATCCAGAACCATGAACGTGATCGATTCCAATATTTCGAAGCGGGATGCAGGCGGA
AAACCGCACCACACTTTTCC

Product: ATP-dependent protease ATP-binding subunit HslU

Products: NA

Alternate protein names: Unfoldase HslU [H]

Number of amino acids: Translated: 433; Mature: 432

Protein sequence:

>433_residues
MTDISPREIVSELDRFIVGQADAKRAVSIALRNRWRRQQLTGPLREEVLPKNILMIGPTGVGKTEIARRLAKLANAPFLK
IEATKFTEVGYVGRDVEQIVRDIVEVAINQTRERKRKDVQARAQLAAEERVLDALVGANASAATRDSFRKRLRSGELNDK
EIEIETQTSGGGPMFEIPGMPGAQVGAISIGDIFGKMGGRTKTRRLTVSDSYEILINEESDKLLDSDQLTQEAIAAVENN
GIVFLDEIDKICVRDGRSGGDVSREGVQRDLLPLIEGTTVSTKHGAVKTDHILFIASGAFHIAKPSDLLPELQGRLPIRV
ELDALTRDDLRRILTEPEASLIKQYVALMDTEGVVLDFTDDAVDALADIAVAVNSTVENIGARRLQTVMERVLDDISFTA
PDRNGETVRIDAGYVQKNIGDLAKNADLSRFIL

Sequences:

>Translated_433_residues
MTDISPREIVSELDRFIVGQADAKRAVSIALRNRWRRQQLTGPLREEVLPKNILMIGPTGVGKTEIARRLAKLANAPFLK
IEATKFTEVGYVGRDVEQIVRDIVEVAINQTRERKRKDVQARAQLAAEERVLDALVGANASAATRDSFRKRLRSGELNDK
EIEIETQTSGGGPMFEIPGMPGAQVGAISIGDIFGKMGGRTKTRRLTVSDSYEILINEESDKLLDSDQLTQEAIAAVENN
GIVFLDEIDKICVRDGRSGGDVSREGVQRDLLPLIEGTTVSTKHGAVKTDHILFIASGAFHIAKPSDLLPELQGRLPIRV
ELDALTRDDLRRILTEPEASLIKQYVALMDTEGVVLDFTDDAVDALADIAVAVNSTVENIGARRLQTVMERVLDDISFTA
PDRNGETVRIDAGYVQKNIGDLAKNADLSRFIL
>Mature_432_residues
TDISPREIVSELDRFIVGQADAKRAVSIALRNRWRRQQLTGPLREEVLPKNILMIGPTGVGKTEIARRLAKLANAPFLKI
EATKFTEVGYVGRDVEQIVRDIVEVAINQTRERKRKDVQARAQLAAEERVLDALVGANASAATRDSFRKRLRSGELNDKE
IEIETQTSGGGPMFEIPGMPGAQVGAISIGDIFGKMGGRTKTRRLTVSDSYEILINEESDKLLDSDQLTQEAIAAVENNG
IVFLDEIDKICVRDGRSGGDVSREGVQRDLLPLIEGTTVSTKHGAVKTDHILFIASGAFHIAKPSDLLPELQGRLPIRVE
LDALTRDDLRRILTEPEASLIKQYVALMDTEGVVLDFTDDAVDALADIAVAVNSTVENIGARRLQTVMERVLDDISFTAP
DRNGETVRIDAGYVQKNIGDLAKNADLSRFIL

Specific function: ATPase subunit of a proteasome-like degradation complex; this subunit has chaperone activity. The binding of ATP and its subsequent hydrolysis by HslU are essential for unfolding of protein substrates subsequently hydrolyzed by HslV. HslU recognizes the N

COG id: COG1220

COG function: function code O; ATP-dependent protease HslVU (ClpYQ), ATPase subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ClpX chaperone family. HslU subfamily [H]

Homologues:

Organism=Homo sapiens, GI7242140, Length=109, Percent_Identity=35.7798165137615, Blast_Score=69, Evalue=8e-12,
Organism=Escherichia coli, GI1790366, Length=446, Percent_Identity=58.2959641255605, Blast_Score=509, Evalue=1e-145,
Organism=Escherichia coli, GI1786642, Length=226, Percent_Identity=35.8407079646018, Blast_Score=95, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6319704, Length=141, Percent_Identity=35.4609929078014, Blast_Score=74, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24648289, Length=257, Percent_Identity=31.9066147859922, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24648291, Length=257, Percent_Identity=31.9066147859922, Blast_Score=97, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR019489
- InterPro:   IPR004491 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 47560; Mature: 47429

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDISPREIVSELDRFIVGQADAKRAVSIALRNRWRRQQLTGPLREEVLPKNILMIGPTG
CCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCEEEECCCC
VGKTEIARRLAKLANAPFLKIEATKFTEVGYVGRDVEQIVRDIVEVAINQTRERKRKDVQ
CCHHHHHHHHHHHHCCCEEEEECCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ARAQLAAEERVLDALVGANASAATRDSFRKRLRSGELNDKEIEIETQTSGGGPMFEIPGM
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCEEECCCC
PGAQVGAISIGDIFGKMGGRTKTRRLTVSDSYEILINEESDKLLDSDQLTQEAIAAVENN
CCCCCCEEEHHHHHHHCCCCCEEEEEEECCCEEEEEECCCHHHCCHHHHHHHHHHHHCCC
GIVFLDEIDKICVRDGRSGGDVSREGVQRDLLPLIEGTTVSTKHGAVKTDHILFIASGAF
CEEEEECHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCEEECCCCCCEECEEEEEECCCE
HIAKPSDLLPELQGRLPIRVELDALTRDDLRRILTEPEASLIKQYVALMDTEGVVLDFTD
EECCCHHHHHHHHCCCCEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEECCH
DAVDALADIAVAVNSTVENIGARRLQTVMERVLDDISFTAPDRNGETVRIDAGYVQKNIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEECCCCCCCCEEEEECHHHHHHHH
DLAKNADLSRFIL
HHHHCCCHHHHCC
>Mature Secondary Structure 
TDISPREIVSELDRFIVGQADAKRAVSIALRNRWRRQQLTGPLREEVLPKNILMIGPTG
CCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCEEEECCCC
VGKTEIARRLAKLANAPFLKIEATKFTEVGYVGRDVEQIVRDIVEVAINQTRERKRKDVQ
CCHHHHHHHHHHHHCCCEEEEECCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ARAQLAAEERVLDALVGANASAATRDSFRKRLRSGELNDKEIEIETQTSGGGPMFEIPGM
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCEEECCCC
PGAQVGAISIGDIFGKMGGRTKTRRLTVSDSYEILINEESDKLLDSDQLTQEAIAAVENN
CCCCCCEEEHHHHHHHCCCCCEEEEEEECCCEEEEEECCCHHHCCHHHHHHHHHHHHCCC
GIVFLDEIDKICVRDGRSGGDVSREGVQRDLLPLIEGTTVSTKHGAVKTDHILFIASGAF
CEEEEECHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCEEECCCCCCEECEEEEEECCCE
HIAKPSDLLPELQGRLPIRVELDALTRDDLRRILTEPEASLIKQYVALMDTEGVVLDFTD
EECCCHHHHHHHHCCCCEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEECCH
DAVDALADIAVAVNSTVENIGARRLQTVMERVLDDISFTAPDRNGETVRIDAGYVQKNIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEECCCCCCCCEEEEECHHHHHHHH
DLAKNADLSRFIL
HHHHCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]