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The map label for this gene is yfcG [H]

Identifier: 75674230

GI number: 75674230

Start: 35623

End: 36219

Strand: Direct

Name: yfcG [H]

Synonym: Nwi_0031

Alternate gene names: 75674230

Gene position: 35623-36219 (Clockwise)

Preceding gene: 75674229

Following gene: 75674234

Centisome position: 1.05

GC content: 65.33

Gene sequence:

>597_bases
ATGATGAAACTCTACTGGTCGCCGCGCTCGCGCTCGTTCACCGCGCTCTGGCTGATGGAGGAAACCGGACAACCCTATGA
GCGGGTTCTGATCGACATCTCCACCGGCGCGCAGAAGACGCCGGAGTTTCTCGCCGTCAACCCGATGGGGAAGGTGCCCG
CGCTGACCGACGGCGCTATCGCGATCGCCGAAGCGGCGGCGATCTGCGCCTACGTCGCCGAGCGTTTCCCGGATGCGAAG
CTGGCCCCGCCGCTCGGGGATGCGCGCCGGGCAAAATATCTGCAATGGCTGTTCTTCGGACCGAGTTGCATTGAACCGGC
GCTGCTCCAGATCTTCGCCAAGCTCCAGGTGCCATCGAGCACGGCCGCGTGGGGGGACGCCACGCAGGTGTTCGACGTTC
TCGACAAGGCCGTGGAAAAGGGGCCGTGGATTCTCGGCGACGACTTCTCCGCCGCCGACATCGTGATCGGCTCCGGGCTG
AACTTTGCGATCCGCCAGTTCAAGATGGTGCCGCCGCGGCCAGCTTTCGATCGCTACATCGACCGCTGCGTGGCGCGGCC
GGCGTTTCAACGCGCGGAGAAAATCGCGGCGGGCTGA

Upstream 100 bases:

>100_bases
GGAAAACCGCCGCACACTTTTCCTCATCCCGCTCCCGTGTCGCGATATGGCCATCCCCTCCTGTTTATGCGGGATCGCCT
GAAGAAAGGACCGCTGTTCG

Downstream 100 bases:

>100_bases
TCCACCTCTCCCCTTGTGAGAGCAGATGCGACCGCCGCACCGCTTCGCGCCGGAGCCCTACTTCGCCGTGTCGTTCGGCG
CGCCGTTGCTCATGCCTTCG

Product: glutathione S-transferase

Products: NA

Alternate protein names: GST-like protein yfcG [H]

Number of amino acids: Translated: 198; Mature: 198

Protein sequence:

>198_residues
MMKLYWSPRSRSFTALWLMEETGQPYERVLIDISTGAQKTPEFLAVNPMGKVPALTDGAIAIAEAAAICAYVAERFPDAK
LAPPLGDARRAKYLQWLFFGPSCIEPALLQIFAKLQVPSSTAAWGDATQVFDVLDKAVEKGPWILGDDFSAADIVIGSGL
NFAIRQFKMVPPRPAFDRYIDRCVARPAFQRAEKIAAG

Sequences:

>Translated_198_residues
MMKLYWSPRSRSFTALWLMEETGQPYERVLIDISTGAQKTPEFLAVNPMGKVPALTDGAIAIAEAAAICAYVAERFPDAK
LAPPLGDARRAKYLQWLFFGPSCIEPALLQIFAKLQVPSSTAAWGDATQVFDVLDKAVEKGPWILGDDFSAADIVIGSGL
NFAIRQFKMVPPRPAFDRYIDRCVARPAFQRAEKIAAG
>Mature_198_residues
MMKLYWSPRSRSFTALWLMEETGQPYERVLIDISTGAQKTPEFLAVNPMGKVPALTDGAIAIAEAAAICAYVAERFPDAK
LAPPLGDARRAKYLQWLFFGPSCIEPALLQIFAKLQVPSSTAAWGDATQVFDVLDKAVEKGPWILGDDFSAADIVIGSGL
NFAIRQFKMVPPRPAFDRYIDRCVARPAFQRAEKIAAG

Specific function: Has disulfide bond reductase activity (in vitro). Has low hydroperoxidase activity with cumene hydroperoxide. Has very low glutathione-S-transferase activity (in vitro) [H]

COG id: COG0625

COG function: function code O; Glutathione S-transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1788640, Length=205, Percent_Identity=29.7560975609756, Blast_Score=87, Evalue=8e-19,
Organism=Escherichia coli, GI87081789, Length=184, Percent_Identity=27.7173913043478, Blast_Score=62, Evalue=2e-11,
Organism=Escherichia coli, GI1787923, Length=198, Percent_Identity=25.2525252525253, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: NA

Molecular weight: Translated: 21754; Mature: 21754

Theoretical pI: Translated: 8.01; Mature: 8.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKLYWSPRSRSFTALWLMEETGQPYERVLIDISTGAQKTPEFLAVNPMGKVPALTDGAI
CCEEEECCCCCCEEEEEEECCCCCCHHHEEEECCCCCCCCCCEEEECCCCCCCCCCCCHH
AIAEAAAICAYVAERFPDAKLAPPLGDARRAKYLQWLFFGPSCIEPALLQIFAKLQVPSS
HHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHEEECCHHHHHHHHHHHHHHCCCCC
TAAWGDATQVFDVLDKAVEKGPWILGDDFSAADIVIGSGLNFAIRQFKMVPPRPAFDRYI
CCCCCCHHHHHHHHHHHHHHCCEEECCCCCCCEEEEECCCHHHHHHEECCCCCCHHHHHH
DRCVARPAFQRAEKIAAG
HHHHCCHHHHHHHHHCCC
>Mature Secondary Structure
MMKLYWSPRSRSFTALWLMEETGQPYERVLIDISTGAQKTPEFLAVNPMGKVPALTDGAI
CCEEEECCCCCCEEEEEEECCCCCCHHHEEEECCCCCCCCCCEEEECCCCCCCCCCCCHH
AIAEAAAICAYVAERFPDAKLAPPLGDARRAKYLQWLFFGPSCIEPALLQIFAKLQVPSS
HHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHEEECCHHHHHHHHHHHHHHCCCCC
TAAWGDATQVFDVLDKAVEKGPWILGDDFSAADIVIGSGLNFAIRQFKMVPPRPAFDRYI
CCCCCCHHHHHHHHHHHHHHCCEEECCCCCCCEEEEECCCHHHHHHEECCCCCCHHHHHH
DRCVARPAFQRAEKIAAG
HHHHCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]