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The map label for this gene is pcaF [H]

Identifier: 73539409

GI number: 73539409

Start: 2404863

End: 2406065

Strand: Direct

Name: pcaF [H]

Synonym: Reut_B5587

Alternate gene names: 73539409

Gene position: 2404863-2406065 (Clockwise)

Preceding gene: 73539408

Following gene: 73539410

Centisome position: 88.21

GC content: 67.25

Gene sequence:

>1203_bases
ATGACCGAAGCCTTTATCTGCGACGCCATCCGTACCCCCATCGGCCGTTACGGCGGCAGCCTGTCCGCGGTACGCGCGGA
CGACCTGGGCGCGGTGCCCCTGAAGGCGCTGATGGCGCGCAATCCGAACGTGGACTGGAAAGCCGTCGACGACGTGATCT
ACGGTAATGCCAACCAGGCCGGCGAAGATAACCGCAACGTGGCACGCATGTCGTCGCTGCTGGCCGGTCTGCCGCAGGAC
GTGCCGGGTGCCACCATCAACCGCCTGTGCGGTTCCGGCATGGACGCAATCGGCACGGCCGCGCGCGCCATCAAGGCCGG
CGAAGCGACCCTGATGATCGCCGGCGGCGTGGAAAGCATGAGCCGCGCTCCGTTCGTGATGGGCAAGGCCACCAGCGCGT
TCTCGCGCGATGCGCAGATCTTCGACACCACGATCGGCTGGCGCTTCATCAACCCGGCGATGCGGGCTGCGTATGGCGTT
GACTCGATGCCCGAGACCGCCGAGAACGTCGCGACCGACTACAAGATCAGCCGCGAAGACCAGGATCTGATGGCGCTGCG
CAGCCAGGAAAAGGCCTCGCGCGCACAGGCCGACGGTACGCTGGCCCAGGAAATCACCGCAGTGACGATCGCCCAGAAGA
AGGGCGACCCGATCATCGTCGAGCGTGACGAGCATCCGCGTGCGACCAGCATGGAAGCGCTGGGCAAGCTGCGCGGCGTG
GTCCGTGCTGACGGCACCGTGACTGCCGGCAATGCATCGGGCGTGAACGACGGCGCCTGCGCGATCCTGCTCGCGAGCGA
AGCCGGCATCAAGCAACACGGCCTGACCCCGCGTGCGCGCATCGTCGGTATGGCAACGGCTGGCGTGGCACCGCGTGTGA
TGGGCATCGGCCCGGCACCGGCCACGCAGAAGCTGATGAAGCAGCTCGGCATGACGCTGGATCAGATCGACGTGATCGAA
CTGAACGAAGCGTTCGCCGCGCAAGGCCTGGCCGTGCTGCGCGAACTGGGCATCGCCGACGACGACAAGCGCGTCAACCC
GAACGGCGGCGCGATCGCGCTGGGCCACCCGCTCGGCATGAGCGGCGCGCGTCTGGTGACCACCGCGATGTACCAGCTGC
ATCGCACCGGCGGCCGCTTCGCGCTGTGCACGATGTGCATCGGCGTGGGCCAGGGTATCGCCATGGTGATCGAACGCGTC
TGA

Upstream 100 bases:

>100_bases
CGCGACCTGGTGGAAGGGCTGAGCTTTGACGAGTTGCAGCGCCTGACCGGCGTTACCCTGATCCAGGGCTGACCACTGAT
ATCAAGACCGAGAGAGAGAC

Downstream 100 bases:

>100_bases
CGCTTCGGCATAGCGCATGCGCCGCGGCCCGCTACGGGACGCGGCGCAGTATCGTCTCCCTCCCCCGTGACGGAGCCATG
CTGCAGGGCCGACAGGCTCG

Product: beta-ketoadipyl CoA thiolase

Products: NA

Alternate protein names: 3-oxoadipyl-CoA thiolase [H]

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MTEAFICDAIRTPIGRYGGSLSAVRADDLGAVPLKALMARNPNVDWKAVDDVIYGNANQAGEDNRNVARMSSLLAGLPQD
VPGATINRLCGSGMDAIGTAARAIKAGEATLMIAGGVESMSRAPFVMGKATSAFSRDAQIFDTTIGWRFINPAMRAAYGV
DSMPETAENVATDYKISREDQDLMALRSQEKASRAQADGTLAQEITAVTIAQKKGDPIIVERDEHPRATSMEALGKLRGV
VRADGTVTAGNASGVNDGACAILLASEAGIKQHGLTPRARIVGMATAGVAPRVMGIGPAPATQKLMKQLGMTLDQIDVIE
LNEAFAAQGLAVLRELGIADDDKRVNPNGGAIALGHPLGMSGARLVTTAMYQLHRTGGRFALCTMCIGVGQGIAMVIERV

Sequences:

>Translated_400_residues
MTEAFICDAIRTPIGRYGGSLSAVRADDLGAVPLKALMARNPNVDWKAVDDVIYGNANQAGEDNRNVARMSSLLAGLPQD
VPGATINRLCGSGMDAIGTAARAIKAGEATLMIAGGVESMSRAPFVMGKATSAFSRDAQIFDTTIGWRFINPAMRAAYGV
DSMPETAENVATDYKISREDQDLMALRSQEKASRAQADGTLAQEITAVTIAQKKGDPIIVERDEHPRATSMEALGKLRGV
VRADGTVTAGNASGVNDGACAILLASEAGIKQHGLTPRARIVGMATAGVAPRVMGIGPAPATQKLMKQLGMTLDQIDVIE
LNEAFAAQGLAVLRELGIADDDKRVNPNGGAIALGHPLGMSGARLVTTAMYQLHRTGGRFALCTMCIGVGQGIAMVIERV
>Mature_399_residues
TEAFICDAIRTPIGRYGGSLSAVRADDLGAVPLKALMARNPNVDWKAVDDVIYGNANQAGEDNRNVARMSSLLAGLPQDV
PGATINRLCGSGMDAIGTAARAIKAGEATLMIAGGVESMSRAPFVMGKATSAFSRDAQIFDTTIGWRFINPAMRAAYGVD
SMPETAENVATDYKISREDQDLMALRSQEKASRAQADGTLAQEITAVTIAQKKGDPIIVERDEHPRATSMEALGKLRGVV
RADGTVTAGNASGVNDGACAILLASEAGIKQHGLTPRARIVGMATAGVAPRVMGIGPAPATQKLMKQLGMTLDQIDVIEL
NEAFAAQGLAVLRELGIADDDKRVNPNGGAIALGHPLGMSGARLVTTAMYQLHRTGGRFALCTMCIGVGQGIAMVIERV

Specific function: Catalyzes thiolytic cleavage of beta-ketoadipyl-CoA to succinyl-CoA and acetyl-CoA [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI167614485, Length=397, Percent_Identity=42.8211586901763, Blast_Score=292, Evalue=4e-79,
Organism=Homo sapiens, GI148539872, Length=398, Percent_Identity=40.2010050251256, Blast_Score=271, Evalue=6e-73,
Organism=Homo sapiens, GI4557237, Length=401, Percent_Identity=35.4114713216958, Blast_Score=226, Evalue=3e-59,
Organism=Homo sapiens, GI4501853, Length=399, Percent_Identity=38.8471177944862, Blast_Score=223, Evalue=3e-58,
Organism=Homo sapiens, GI4504327, Length=427, Percent_Identity=30.6791569086651, Blast_Score=178, Evalue=9e-45,
Organism=Homo sapiens, GI194353979, Length=390, Percent_Identity=28.974358974359, Blast_Score=128, Evalue=1e-29,
Organism=Escherichia coli, GI1787663, Length=401, Percent_Identity=67.8304239401496, Blast_Score=558, Evalue=1e-160,
Organism=Escherichia coli, GI1788554, Length=403, Percent_Identity=47.1464019851117, Blast_Score=303, Evalue=1e-83,
Organism=Escherichia coli, GI87082165, Length=401, Percent_Identity=43.142144638404, Blast_Score=282, Evalue=3e-77,
Organism=Escherichia coli, GI48994986, Length=405, Percent_Identity=42.4691358024691, Blast_Score=278, Evalue=5e-76,
Organism=Escherichia coli, GI1788683, Length=424, Percent_Identity=31.1320754716981, Blast_Score=168, Evalue=5e-43,
Organism=Caenorhabditis elegans, GI133906874, Length=401, Percent_Identity=43.8902743142145, Blast_Score=322, Evalue=3e-88,
Organism=Caenorhabditis elegans, GI25147385, Length=398, Percent_Identity=34.9246231155779, Blast_Score=228, Evalue=3e-60,
Organism=Caenorhabditis elegans, GI17535921, Length=400, Percent_Identity=35.5, Blast_Score=207, Evalue=1e-53,
Organism=Caenorhabditis elegans, GI17551802, Length=432, Percent_Identity=30.0925925925926, Blast_Score=171, Evalue=9e-43,
Organism=Caenorhabditis elegans, GI17535917, Length=405, Percent_Identity=29.6296296296296, Blast_Score=159, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6325229, Length=400, Percent_Identity=38.5, Blast_Score=241, Evalue=2e-64,
Organism=Saccharomyces cerevisiae, GI6322031, Length=412, Percent_Identity=34.7087378640777, Blast_Score=206, Evalue=5e-54,
Organism=Drosophila melanogaster, GI24655093, Length=401, Percent_Identity=44.6384039900249, Blast_Score=320, Evalue=8e-88,
Organism=Drosophila melanogaster, GI17648125, Length=403, Percent_Identity=40.4466501240695, Blast_Score=266, Evalue=1e-71,
Organism=Drosophila melanogaster, GI24640423, Length=405, Percent_Identity=34.0740740740741, Blast_Score=219, Evalue=2e-57,
Organism=Drosophila melanogaster, GI17137578, Length=424, Percent_Identity=32.0754716981132, Blast_Score=179, Evalue=3e-45,
Organism=Drosophila melanogaster, GI19921506, Length=320, Percent_Identity=24.6875, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012793
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.174 [H]

Molecular weight: Translated: 41817; Mature: 41685

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEAFICDAIRTPIGRYGGSLSAVRADDLGAVPLKALMARNPNVDWKAVDDVIYGNANQA
CCCHHHHHHHHCCHHHCCCCEEEEECCCCCCHHHHHHHHCCCCCCCEEHHHHHCCCCCCC
GEDNRNVARMSSLLAGLPQDVPGATINRLCGSGMDAIGTAARAIKAGEATLMIAGGVESM
CCCCHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHCCCEEEEEECCHHHH
SRAPFVMGKATSAFSRDAQIFDTTIGWRFINPAMRAAYGVDSMPETAENVATDYKISRED
CCCCEEEECCHHHHCCCCHHEEHHCCCEECCHHHHHHHCCCCCCHHHHHHHHCCEECCCC
QDLMALRSQEKASRAQADGTLAQEITAVTIAQKKGDPIIVERDEHPRATSMEALGKLRGV
HHHHHHHHHHHHHHHHCCCHHHHHHHEEEEEECCCCEEEEECCCCCCHHHHHHHHHHHHH
VRADGTVTAGNASGVNDGACAILLASEAGIKQHGLTPRARIVGMATAGVAPRVMGIGPAP
EECCCEEECCCCCCCCCCCEEEEEECCCCCHHCCCCCHHHEEEEECCCCCCCEEECCCCH
ATQKLMKQLGMTLDQIDVIELNEAFAAQGLAVLRELGIADDDKRVNPNGGAIALGHPLGM
HHHHHHHHHCCCHHCEEEEECCHHHHHHHHHHHHHCCCCCCCCEECCCCCEEEECCCCCC
SGARLVTTAMYQLHRTGGRFALCTMCIGVGQGIAMVIERV
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHCC
>Mature Secondary Structure 
TEAFICDAIRTPIGRYGGSLSAVRADDLGAVPLKALMARNPNVDWKAVDDVIYGNANQA
CCHHHHHHHHCCHHHCCCCEEEEECCCCCCHHHHHHHHCCCCCCCEEHHHHHCCCCCCC
GEDNRNVARMSSLLAGLPQDVPGATINRLCGSGMDAIGTAARAIKAGEATLMIAGGVESM
CCCCHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHCCCEEEEEECCHHHH
SRAPFVMGKATSAFSRDAQIFDTTIGWRFINPAMRAAYGVDSMPETAENVATDYKISRED
CCCCEEEECCHHHHCCCCHHEEHHCCCEECCHHHHHHHCCCCCCHHHHHHHHCCEECCCC
QDLMALRSQEKASRAQADGTLAQEITAVTIAQKKGDPIIVERDEHPRATSMEALGKLRGV
HHHHHHHHHHHHHHHHCCCHHHHHHHEEEEEECCCCEEEEECCCCCCHHHHHHHHHHHHH
VRADGTVTAGNASGVNDGACAILLASEAGIKQHGLTPRARIVGMATAGVAPRVMGIGPAP
EECCCEEECCCCCCCCCCCEEEEEECCCCCHHCCCCCHHHEEEEECCCCCCCEEECCCCH
ATQKLMKQLGMTLDQIDVIELNEAFAAQGLAVLRELGIADDDKRVNPNGGAIALGHPLGM
HHHHHHHHHCCCHHCEEEEECCHHHHHHHHHHHHHCCCCCCCCEECCCCCEEEECCCCCC
SGARLVTTAMYQLHRTGGRFALCTMCIGVGQGIAMVIERV
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]