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The map label for this gene is pcaJ [H]

Identifier: 73539408

GI number: 73539408

Start: 2404196

End: 2404834

Strand: Direct

Name: pcaJ [H]

Synonym: Reut_B5586

Alternate gene names: 73539408

Gene position: 2404196-2404834 (Clockwise)

Preceding gene: 73539407

Following gene: 73539409

Centisome position: 88.19

GC content: 64.48

Gene sequence:

>639_bases
ATGCAAAAACTGACCCGCGACCAAATGGCCGCCCGCGTGGCCAAAGACATCCCCGAAGGTGCCGTGGTGAACCTCGGCAT
CGGACTGCCCACGCTGGTGGGCAACCACCTGCCCGCAGACAAGGAAATCCTGCTGCACAGCGAGAATGGCCTGCTCGGCA
TGGGCCCCGCGCCCGCGGCCGGTGAAGAAGACGGCGACCTGATCAACGCCGGCAAGCAGCCAGTGACGATCAAGGCTGGC
GGTTCCTATTTCCATCACGCCGATTCGTTCGCCATGATGCGCGGCGGCCACCTCGACTTCTGCGTGCTGGGTGCGTTCCA
GGTCTCCGAGAAGGGCGACCTGGCCAACTGGCACACCGGCGCACCGGGCGCCATCCCCGCCGTTGGCGGTGCGATGGACC
TCGCCATCGGGGCCAAGCAGGTGTTCGTGATGATGGAACACCTTACCAAGCAGGGCGAAAGCAAGATCGTGCCGCAGTGC
TCCTATCCGCTGACGGGCATCGGTTGCGTGACGCGCATCTACACTGATCTGGCCACCATCGACGTAACCACCGACGGGCT
GGTTGCGCGCGACCTGGTGGAAGGGCTGAGCTTTGACGAGTTGCAGCGCCTGACCGGCGTTACCCTGATCCAGGGCTGA

Upstream 100 bases:

>100_bases
AGCCACGTGGTGGAACTGGGCGAGCTGGATCCCGAGCACATCGTGACGCCGGGCCTGTTCGTCAAGCGCGTGGTGAAGGT
CTGAGCGAAGGAGAGCGAAC

Downstream 100 bases:

>100_bases
CCACTGATATCAAGACCGAGAGAGAGACATGACCGAAGCCTTTATCTGCGACGCCATCCGTACCCCCATCGGCCGTTACG
GCGGCAGCCTGTCCGCGGTA

Product: butyryl-CoA:acetate CoA transferase

Products: NA

Alternate protein names: Beta-ketoadipate:succinyl-CoA transferase subunit B [H]

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MQKLTRDQMAARVAKDIPEGAVVNLGIGLPTLVGNHLPADKEILLHSENGLLGMGPAPAAGEEDGDLINAGKQPVTIKAG
GSYFHHADSFAMMRGGHLDFCVLGAFQVSEKGDLANWHTGAPGAIPAVGGAMDLAIGAKQVFVMMEHLTKQGESKIVPQC
SYPLTGIGCVTRIYTDLATIDVTTDGLVARDLVEGLSFDELQRLTGVTLIQG

Sequences:

>Translated_212_residues
MQKLTRDQMAARVAKDIPEGAVVNLGIGLPTLVGNHLPADKEILLHSENGLLGMGPAPAAGEEDGDLINAGKQPVTIKAG
GSYFHHADSFAMMRGGHLDFCVLGAFQVSEKGDLANWHTGAPGAIPAVGGAMDLAIGAKQVFVMMEHLTKQGESKIVPQC
SYPLTGIGCVTRIYTDLATIDVTTDGLVARDLVEGLSFDELQRLTGVTLIQG
>Mature_212_residues
MQKLTRDQMAARVAKDIPEGAVVNLGIGLPTLVGNHLPADKEILLHSENGLLGMGPAPAAGEEDGDLINAGKQPVTIKAG
GSYFHHADSFAMMRGGHLDFCVLGAFQVSEKGDLANWHTGAPGAIPAVGGAMDLAIGAKQVFVMMEHLTKQGESKIVPQC
SYPLTGIGCVTRIYTDLATIDVTTDGLVARDLVEGLSFDELQRLTGVTLIQG

Specific function: SHORT-CHAIN FATTY ACIDS METABOLISM. [C]

COG id: COG2057

COG function: function code I; Acyl CoA:acetate/3-ketoacid CoA transferase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-oxoacid CoA-transferase subunit B family [H]

Homologues:

Organism=Homo sapiens, GI4557817, Length=204, Percent_Identity=48.5294117647059, Blast_Score=181, Evalue=6e-46,
Organism=Homo sapiens, GI11545841, Length=207, Percent_Identity=47.3429951690821, Blast_Score=181, Evalue=6e-46,
Organism=Escherichia coli, GI1788552, Length=176, Percent_Identity=47.7272727272727, Blast_Score=163, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI17531517, Length=209, Percent_Identity=47.8468899521531, Blast_Score=186, Evalue=6e-48,
Organism=Drosophila melanogaster, GI21358325, Length=210, Percent_Identity=44.2857142857143, Blast_Score=171, Evalue=4e-43,
Organism=Drosophila melanogaster, GI24655794, Length=210, Percent_Identity=44.2857142857143, Blast_Score=170, Evalue=5e-43,

Paralogues:

None

Copy number: 369 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012791
- InterPro:   IPR004165
- InterPro:   IPR004164 [H]

Pfam domain/function: PF01144 CoA_trans [H]

EC number: =2.8.3.6 [H]

Molecular weight: Translated: 22216; Mature: 22216

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS01274 COA_TRANSF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKLTRDQMAARVAKDIPEGAVVNLGIGLPTLVGNHLPADKEILLHSENGLLGMGPAPAA
CCCCHHHHHHHHHHHHCCCCCEEEECCCCHHHHCCCCCCCCEEEEECCCCEEECCCCCCC
GEEDGDLINAGKQPVTIKAGGSYFHHADSFAMMRGGHLDFCVLGAFQVSEKGDLANWHTG
CCCCCCEECCCCCCEEEEECCCEECCCCCHHHHCCCCCCEEEEEEEEECCCCCCCCCCCC
APGAIPAVGGAMDLAIGAKQVFVMMEHLTKQGESKIVPQCSYPLTGIGCVTRIYTDLATI
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHEE
DVTTDGLVARDLVEGLSFDELQRLTGVTLIQG
EECCCCHHHHHHHHCCCHHHHHHHHCEEEECC
>Mature Secondary Structure
MQKLTRDQMAARVAKDIPEGAVVNLGIGLPTLVGNHLPADKEILLHSENGLLGMGPAPAA
CCCCHHHHHHHHHHHHCCCCCEEEECCCCHHHHCCCCCCCCEEEEECCCCEEECCCCCCC
GEEDGDLINAGKQPVTIKAGGSYFHHADSFAMMRGGHLDFCVLGAFQVSEKGDLANWHTG
CCCCCCEECCCCCCEEEEECCCEECCCCCHHHHCCCCCCEEEEEEEEECCCCCCCCCCCC
APGAIPAVGGAMDLAIGAKQVFVMMEHLTKQGESKIVPQCSYPLTGIGCVTRIYTDLATI
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHEE
DVTTDGLVARDLVEGLSFDELQRLTGVTLIQG
EECCCCHHHHHHHHCCCHHHHHHHHCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]