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The map label for this gene is amnE [H]

Identifier: 73539322

GI number: 73539322

Start: 2297463

End: 2298263

Strand: Direct

Name: amnE [H]

Synonym: Reut_B5500

Alternate gene names: 73539322

Gene position: 2297463-2298263 (Clockwise)

Preceding gene: 73539321

Following gene: 73539323

Centisome position: 84.27

GC content: 66.29

Gene sequence:

>801_bases
ATGACCGACATGAGCCAGACGATCGTCGACGCGGCGCTCGCGCTCGACAACGCCGCCATGCAGGCGCAGCCGATGCCGCA
GTTCGAAGCCGGCCATTTCGATTTGCCGACGGCGTATGCCATCCAGCGCGCGTCGATCCAGCACCGCGTCACACGCGGCG
AACAGGTCAAGGGCATCAAGCTTGGTTTCACCAGCCGCGCGAAGATGGTGCAGATGGGCGTCGACAGCCTGATCTGGGGC
TTCCTTACCGACGCGATGCTCGAGGAAGATGGCGGTACGGTGCACCTTGGGCGCTATATCCATCCGCGCGTGGAGCCCGA
AGTCTGCTTCGTGACGCGCCGGACCATCGACGCGCCGCTGACGGCGCTGGAAGCGGCGGACTATCTCGAAGCGGTCGCGC
CGGCGATGGAGATCATCGATTCGCGCTACCGCGATTTCCGCTTCAGCCTGGAAGATGTCGTGGCCGACAATTGTTCGTCG
GCCGGACTCGTGGTGGGTGCGTGGTCGCGCGACTTTGCGGGGCTTGCGAACCTAGGCGTACGCATGCAGTTCGATGGCCG
TGATGTGCAGGTCGGCTCGACAGGCGCGATTCTCGGCCATCCGCTGCGCACCGTCGTGCAGGCATCACGCCTGCTGCACG
CGGCGCAGGTCAGCCTGCCGGCGGGCTCGCTGATCATGGCCGGCGCGGCCACCGCGGCCGAGGCGTTGCGCGCGGGGCTG
CATGTGAGTTGCCATATCGGCAGCGGCGTCAACACGCTGGGCAGCGTGTCGTTCACTACGCGTGCGGGCATGCCAGACTG
A

Upstream 100 bases:

>100_bases
GCGACTGTGCAGCCGGGCAATGTTTTCACCGCACGTATGGAAGGCCTCGGTGCCGTGACCGCCGCGTTCCGCGCCGATAG
CCAGCAAGGAGATGCCTGAG

Downstream 100 bases:

>100_bases
CTGCCGTATCCGCATCGACAACGCATTCAACAGCTACCAGGAGACCAACCATGCCGACAGAGGCGCATCTGGTGCAGGGC
AAGGCAAAGCCACGGGGCCG

Product: 4-oxalocrotonate decarboxylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 266; Mature: 265

Protein sequence:

>266_residues
MTDMSQTIVDAALALDNAAMQAQPMPQFEAGHFDLPTAYAIQRASIQHRVTRGEQVKGIKLGFTSRAKMVQMGVDSLIWG
FLTDAMLEEDGGTVHLGRYIHPRVEPEVCFVTRRTIDAPLTALEAADYLEAVAPAMEIIDSRYRDFRFSLEDVVADNCSS
AGLVVGAWSRDFAGLANLGVRMQFDGRDVQVGSTGAILGHPLRTVVQASRLLHAAQVSLPAGSLIMAGAATAAEALRAGL
HVSCHIGSGVNTLGSVSFTTRAGMPD

Sequences:

>Translated_266_residues
MTDMSQTIVDAALALDNAAMQAQPMPQFEAGHFDLPTAYAIQRASIQHRVTRGEQVKGIKLGFTSRAKMVQMGVDSLIWG
FLTDAMLEEDGGTVHLGRYIHPRVEPEVCFVTRRTIDAPLTALEAADYLEAVAPAMEIIDSRYRDFRFSLEDVVADNCSS
AGLVVGAWSRDFAGLANLGVRMQFDGRDVQVGSTGAILGHPLRTVVQASRLLHAAQVSLPAGSLIMAGAATAAEALRAGL
HVSCHIGSGVNTLGSVSFTTRAGMPD
>Mature_265_residues
TDMSQTIVDAALALDNAAMQAQPMPQFEAGHFDLPTAYAIQRASIQHRVTRGEQVKGIKLGFTSRAKMVQMGVDSLIWGF
LTDAMLEEDGGTVHLGRYIHPRVEPEVCFVTRRTIDAPLTALEAADYLEAVAPAMEIIDSRYRDFRFSLEDVVADNCSSA
GLVVGAWSRDFAGLANLGVRMQFDGRDVQVGSTGAILGHPLRTVVQASRLLHAAQVSLPAGSLIMAGAATAAEALRAGLH
VSCHIGSGVNTLGSVSFTTRAGMPD

Specific function: Involved in the modified meta-cleavage pathway for the 2-aminophenol catabolism [H]

COG id: COG3971

COG function: function code Q; 2-keto-4-pentenoate hydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hydratase/decarboxylase family [H]

Homologues:

Organism=Escherichia coli, GI87081722, Length=231, Percent_Identity=33.7662337662338, Blast_Score=115, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002529
- InterPro:   IPR011234 [H]

Pfam domain/function: PF01557 FAA_hydrolase [H]

EC number: =4.1.1.77 [H]

Molecular weight: Translated: 28354; Mature: 28223

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDMSQTIVDAALALDNAAMQAQPMPQFEAGHFDLPTAYAIQRASIQHRVTRGEQVKGIK
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEE
LGFTSRAKMVQMGVDSLIWGFLTDAMLEEDGGTVHLGRYIHPRVEPEVCFVTRRTIDAPL
ECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCEECCCCCCCEEEEEHHCCCCHH
TALEAADYLEAVAPAMEIIDSRYRDFRFSLEDVVADNCSSAGLVVGAWSRDFAGLANLGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHCCCE
RMQFDGRDVQVGSTGAILGHPLRTVVQASRLLHAAQVSLPAGSLIMAGAATAAEALRAGL
EEEECCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHCCC
HVSCHIGSGVNTLGSVSFTTRAGMPD
EEEEEECCCCCCCCCEEEEECCCCCC
>Mature Secondary Structure 
TDMSQTIVDAALALDNAAMQAQPMPQFEAGHFDLPTAYAIQRASIQHRVTRGEQVKGIK
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEE
LGFTSRAKMVQMGVDSLIWGFLTDAMLEEDGGTVHLGRYIHPRVEPEVCFVTRRTIDAPL
ECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCEECCCCCCCEEEEEHHCCCCHH
TALEAADYLEAVAPAMEIIDSRYRDFRFSLEDVVADNCSSAGLVVGAWSRDFAGLANLGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHCCCE
RMQFDGRDVQVGSTGAILGHPLRTVVQASRLLHAAQVSLPAGSLIMAGAATAAEALRAGL
EEEECCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHCCC
HVSCHIGSGVNTLGSVSFTTRAGMPD
EEEEEECCCCCCCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9169437; 11081795 [H]