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The map label for this gene is todJ [H]

Identifier: 73539321

GI number: 73539321

Start: 2296637

End: 2297461

Strand: Direct

Name: todJ [H]

Synonym: Reut_B5499

Alternate gene names: 73539321

Gene position: 2296637-2297461 (Clockwise)

Preceding gene: 73539320

Following gene: 73539322

Centisome position: 84.24

GC content: 67.15

Gene sequence:

>825_bases
ATGCTGAACCCTGAACTTATCGAGGCCGCGGACCAGCTCTGGTTCGCTGCGGAACGCCGCCAGCCGTGCCTGCCCGTGCG
CGATGCCATTGCACACGCCAGCACCGGTGGCGCGGACCCGATCGCGCAGGCCTATGCGATCCAGCAGTACAACACCGAGC
GCGCGCTGCGCGCCGGGCGCCGCCTGGTTGGCCGCAAGATTGGGCTGACCGCAAAGTCGGTGCAGCGCCAGCTTGGCGTG
GACCAGCCGGACTTCGGCATGCTGTTCGCCGATATGGCGCGACAGGACGGCGAAGACATCGACTGCGCCGATGTCCTGCA
GCCAAAGGTCGAGGCCGAAGTCGCGCTGGTGCTCGAACGCGATCTGCCAGATGAGGTGAACACCGTAGCGGACGTGATCC
GTGCCACGGCCTTTGCGCTGCCGGCCATCGAAATCGTCGGCAGCCGCGTGGCCGGGTGGGATATCCGTCTGACCGACACC
ATTGCCGACAACGCATCGTCGGGCCTGTTCGTGCTGGGCACGCGGCCGGTATTGCTCGGTGCGCTGGACCTGGCCAACTG
CGGCATGAGCATGGAGCTCAATGGGGATCCGGTGTCGGTCGGCGCCGGCGCCGCTTGCCTGGGCAATCCGCTCAATGCGG
CAGTGTGGCTTGCCAACACCATGAGCCGCGTCGGTGCGCCGCTCAAGGCCGGCGACGTGATCCTGACGGGGGCACTCGGC
CCGATGGCGACTGTGCAGCCGGGCAATGTTTTCACCGCACGTATGGAAGGCCTCGGTGCCGTGACCGCCGCGTTCCGCGC
CGATAGCCAGCAAGGAGATGCCTGA

Upstream 100 bases:

>100_bases
CGGTGGTACCCGTCTGTCGGGCATCGGGCGCGAAGGCGGGCGCCATTCGCTGGACTTCTATTCGTCCACCACCACCATCT
GCGTCAACCCGTGAGCCCGC

Downstream 100 bases:

>100_bases
GATGACCGACATGAGCCAGACGATCGTCGACGCGGCGCTCGCGCTCGACAACGCCGCCATGCAGGCGCAGCCGATGCCGC
AGTTCGAAGCCGGCCATTTC

Product: 4-oxalocrotonate decarboxylase

Products: 2-oxopent-4-enoate; H2O

Alternate protein names: NA

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MLNPELIEAADQLWFAAERRQPCLPVRDAIAHASTGGADPIAQAYAIQQYNTERALRAGRRLVGRKIGLTAKSVQRQLGV
DQPDFGMLFADMARQDGEDIDCADVLQPKVEAEVALVLERDLPDEVNTVADVIRATAFALPAIEIVGSRVAGWDIRLTDT
IADNASSGLFVLGTRPVLLGALDLANCGMSMELNGDPVSVGAGAACLGNPLNAAVWLANTMSRVGAPLKAGDVILTGALG
PMATVQPGNVFTARMEGLGAVTAAFRADSQQGDA

Sequences:

>Translated_274_residues
MLNPELIEAADQLWFAAERRQPCLPVRDAIAHASTGGADPIAQAYAIQQYNTERALRAGRRLVGRKIGLTAKSVQRQLGV
DQPDFGMLFADMARQDGEDIDCADVLQPKVEAEVALVLERDLPDEVNTVADVIRATAFALPAIEIVGSRVAGWDIRLTDT
IADNASSGLFVLGTRPVLLGALDLANCGMSMELNGDPVSVGAGAACLGNPLNAAVWLANTMSRVGAPLKAGDVILTGALG
PMATVQPGNVFTARMEGLGAVTAAFRADSQQGDA
>Mature_274_residues
MLNPELIEAADQLWFAAERRQPCLPVRDAIAHASTGGADPIAQAYAIQQYNTERALRAGRRLVGRKIGLTAKSVQRQLGV
DQPDFGMLFADMARQDGEDIDCADVLQPKVEAEVALVLERDLPDEVNTVADVIRATAFALPAIEIVGSRVAGWDIRLTDT
IADNASSGLFVLGTRPVLLGALDLANCGMSMELNGDPVSVGAGAACLGNPLNAAVWLANTMSRVGAPLKAGDVILTGALG
PMATVQPGNVFTARMEGLGAVTAAFRADSQQGDA

Specific function: Converts the product of 2-hydroxy-6-oxo-2,4- heptadienoate hydrolase [H]

COG id: COG3971

COG function: function code Q; 2-keto-4-pentenoate hydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hydratase/decarboxylase family [H]

Homologues:

Organism=Escherichia coli, GI87081722, Length=272, Percent_Identity=50, Blast_Score=257, Evalue=7e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002529
- InterPro:   IPR011234 [H]

Pfam domain/function: PF01557 FAA_hydrolase [H]

EC number: 4.2.1.80

Molecular weight: Translated: 28689; Mature: 28689

Theoretical pI: Translated: 4.35; Mature: 4.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNPELIEAADQLWFAAERRQPCLPVRDAIAHASTGGADPIAQAYAIQQYNTERALRAGR
CCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHCHHHHHHHHH
RLVGRKIGLTAKSVQRQLGVDQPDFGMLFADMARQDGEDIDCADVLQPKVEAEVALVLER
HHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCHHHHHCCHHHHHEEEEEEC
DLPDEVNTVADVIRATAFALPAIEIVGSRVAGWDIRLTDTIADNASSGLFVLGTRPVLLG
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHCCCCCCCEEEEECCHHHHH
ALDLANCGMSMELNGDPVSVGAGAACLGNPLNAAVWLANTMSRVGAPLKAGDVILTGALG
HHHHHHCCEEEEECCCCEECCCCHHHHCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCC
PMATVQPGNVFTARMEGLGAVTAAFRADSQQGDA
CCCCCCCCCEEEEHHHCCHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MLNPELIEAADQLWFAAERRQPCLPVRDAIAHASTGGADPIAQAYAIQQYNTERALRAGR
CCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHCHHHHHHHHH
RLVGRKIGLTAKSVQRQLGVDQPDFGMLFADMARQDGEDIDCADVLQPKVEAEVALVLER
HHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCHHHHHCCHHHHHEEEEEEC
DLPDEVNTVADVIRATAFALPAIEIVGSRVAGWDIRLTDTIADNASSGLFVLGTRPVLLG
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHCCCCCCCEEEEECCHHHHH
ALDLANCGMSMELNGDPVSVGAGAACLGNPLNAAVWLANTMSRVGAPLKAGDVILTGALG
HHHHHHCCEEEEECCCCEECCCCHHHHCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCC
PMATVQPGNVFTARMEGLGAVTAAFRADSQQGDA
CCCCCCCCCEEEEHHHCCHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 4-hydroxy-2-oxopentanoate

Specific reaction: 4-hydroxy-2-oxopentanoate = 2-oxopent-4-enoate + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2670929 [H]