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The map label for this gene is glcA [H]

Identifier: 73539267

GI number: 73539267

Start: 2236094

End: 2237803

Strand: Reverse

Name: glcA [H]

Synonym: Reut_B5445

Alternate gene names: 73539267

Gene position: 2237803-2236094 (Counterclockwise)

Preceding gene: 73539268

Following gene: 73539266

Centisome position: 82.09

GC content: 66.08

Gene sequence:

>1710_bases
GTGCAACCCTGGACCCAAATCTATATGCCGCTGGGCAGCCTCTGGCTGTCCTCACTGGCGGCAGCCATCCCCATCCTGTT
CTTCTTCATCGCGCTGGCCGTGTTGCGCCTGAAAGGCCATGTCGCGGCGGCGGGAACGCTGTTGCTTTCGCTGGCCGTGG
CGGTCTTTGCGTACGGCATGCCGGCGCAGCAGGCGCTTGCTGCAGCGGGTTTCGGCTTTGCCTACGGGCTGTGGCCGATC
GCATGGATCATCGTGACAGCGGTGTTCCTCTACAAGATCGTGGTCAAGACCGGCCAGTTCGACGTCATCCGCGCGTCGGT
GCTGTCGATCACCGATGACCAGCGCCTGCAGATGCTGCTGATCGGCTTCGCCTTTGGCGCGTTCCTGGAAGGTGCGGCGG
GCTTCGGTGCGCCGGTGGCCATTACCGCTGCGCTGCTGGTGGGCCTTGGCTTCAATCCGCTCTACGCGGCAGGCCTTTGC
CTGATCGCCAACACCGCGCCGGTGGCCTTCGGTGCGATGGGCATTCCCATCATCGTCGCCGGGCAGGTGACGGGCATCGA
CGCCATGCACATCGGCGCGATGGCCGGGCGCCAGCTTCCGCTGCTGTCGCTGATGGTGCCGTTCTGGCTCGTGTTCATGA
TGGACGGCGCCAAGGGCGTGCGCGAGACGTGGCCAGCCGCGCTGGTGACGGGCGGCAGCTTTGCCGTGACGCAGTACTTC
ACGTCGAACCATATCGGGCCCGAGTTGCCGGACATTACCTCGGCCCTGGTCAGCCTGGTGTCGCTGGCGGCCTTCCTGAA
GGTGTGGCAGCCGCGCAGCGCGTCGCAACGCGCTGGCGGCAGCGTTGGGGGCGGCGCCATGGCGATGTCGGCATTCGGCG
GCGGCGGCTTCAGCAACGGCGTGCAGAACGGCGGCAATCGCAAGTCGCCGTACACCACGGCGCAGACAATCCGTGCGTGG
GCGCCGTTCGGCATCCTGACGGCTATCGTCACGGTATGGAGCCTGCAGCCGTTCAAGGCGCTGTTCGCCGGTAACGGTGC
GCTGGCCGGTACGGTGCTGAAGTTCAAGGTGCCGGGGCTGGACCAGCTGGTCCTCAAGGCCGCGCCGATCGTCGCTTCGC
CCAAGCCGTACGAAGCTGTGCTCAAGCTCGACCTGCTGTCCGCCGTTGGCACCGCCATCCTGCTGACTGCGCTGATCTCG
GCCGTGCTGCTGCGCATGAAGCCGCGCGACCTGGTGACCACGTTCGGCGAAACGCTGGTGGAATTGGGCCGCCCGATCCT
GTCGATCGGACTGGTGCTGGCGTTTGCGTTCGTGGCCAACTACTCGGGCATGTCGTCCACGCTGGCGCTGCTGCTGGCCG
GTACGGGCGCCGCATTCCCGTTCTTCTCGCCGTTCCTCGGCTGGCTCGGCGTCTTCCTGACGGGTTCGGACACGTCGTCG
AACGCGCTGTTCTGTTCGCTGCAGAACACTACGGCGCATCAGATCGGCGTGTCGGACACGCTGCTGGTCGCCGCCAACAC
CACGGGCGGCGTGACGGCCAAGATGATCTCGCCGCAATCGATCGCGGTGGCCTGCGCGGCGACGGGGCTCGTCGGCAAGG
AGTCCGAACTGTTCCGCTTCACCGTCAAGCACAGCCTGCTGTTCGCGGTGATCGTCGGCATCATCACCGTGGTACAGGCG
TATCTGCTGCCGGGAATGATCCCCGGTTAA

Upstream 100 bases:

>100_bases
GCTCGCGGGAGAAAAAAAGAACATCGTCGCGACGGACACGGCCGCGTGGCGCCAACAACAACAAGGATTTCCCGAAACAC
CATTGAAGTAGGAGACGAGC

Downstream 100 bases:

>100_bases
GCCTCTCCGGCCCAGGGCGGGTTTCGTCGCATCCGACGATGCCCGCCGACTATGCGGCCTCTTAGCATGATCTGATCCGC
CCGAGCACAAGGGCCGGCTC

Product: L-lactate permease

Products: lactate [Cytoplasm]; Proton [Cytoplasm]; glycolate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 569; Mature: 569

Protein sequence:

>569_residues
MQPWTQIYMPLGSLWLSSLAAAIPILFFFIALAVLRLKGHVAAAGTLLLSLAVAVFAYGMPAQQALAAAGFGFAYGLWPI
AWIIVTAVFLYKIVVKTGQFDVIRASVLSITDDQRLQMLLIGFAFGAFLEGAAGFGAPVAITAALLVGLGFNPLYAAGLC
LIANTAPVAFGAMGIPIIVAGQVTGIDAMHIGAMAGRQLPLLSLMVPFWLVFMMDGAKGVRETWPAALVTGGSFAVTQYF
TSNHIGPELPDITSALVSLVSLAAFLKVWQPRSASQRAGGSVGGGAMAMSAFGGGGFSNGVQNGGNRKSPYTTAQTIRAW
APFGILTAIVTVWSLQPFKALFAGNGALAGTVLKFKVPGLDQLVLKAAPIVASPKPYEAVLKLDLLSAVGTAILLTALIS
AVLLRMKPRDLVTTFGETLVELGRPILSIGLVLAFAFVANYSGMSSTLALLLAGTGAAFPFFSPFLGWLGVFLTGSDTSS
NALFCSLQNTTAHQIGVSDTLLVAANTTGGVTAKMISPQSIAVACAATGLVGKESELFRFTVKHSLLFAVIVGIITVVQA
YLLPGMIPG

Sequences:

>Translated_569_residues
MQPWTQIYMPLGSLWLSSLAAAIPILFFFIALAVLRLKGHVAAAGTLLLSLAVAVFAYGMPAQQALAAAGFGFAYGLWPI
AWIIVTAVFLYKIVVKTGQFDVIRASVLSITDDQRLQMLLIGFAFGAFLEGAAGFGAPVAITAALLVGLGFNPLYAAGLC
LIANTAPVAFGAMGIPIIVAGQVTGIDAMHIGAMAGRQLPLLSLMVPFWLVFMMDGAKGVRETWPAALVTGGSFAVTQYF
TSNHIGPELPDITSALVSLVSLAAFLKVWQPRSASQRAGGSVGGGAMAMSAFGGGGFSNGVQNGGNRKSPYTTAQTIRAW
APFGILTAIVTVWSLQPFKALFAGNGALAGTVLKFKVPGLDQLVLKAAPIVASPKPYEAVLKLDLLSAVGTAILLTALIS
AVLLRMKPRDLVTTFGETLVELGRPILSIGLVLAFAFVANYSGMSSTLALLLAGTGAAFPFFSPFLGWLGVFLTGSDTSS
NALFCSLQNTTAHQIGVSDTLLVAANTTGGVTAKMISPQSIAVACAATGLVGKESELFRFTVKHSLLFAVIVGIITVVQA
YLLPGMIPG
>Mature_569_residues
MQPWTQIYMPLGSLWLSSLAAAIPILFFFIALAVLRLKGHVAAAGTLLLSLAVAVFAYGMPAQQALAAAGFGFAYGLWPI
AWIIVTAVFLYKIVVKTGQFDVIRASVLSITDDQRLQMLLIGFAFGAFLEGAAGFGAPVAITAALLVGLGFNPLYAAGLC
LIANTAPVAFGAMGIPIIVAGQVTGIDAMHIGAMAGRQLPLLSLMVPFWLVFMMDGAKGVRETWPAALVTGGSFAVTQYF
TSNHIGPELPDITSALVSLVSLAAFLKVWQPRSASQRAGGSVGGGAMAMSAFGGGGFSNGVQNGGNRKSPYTTAQTIRAW
APFGILTAIVTVWSLQPFKALFAGNGALAGTVLKFKVPGLDQLVLKAAPIVASPKPYEAVLKLDLLSAVGTAILLTALIS
AVLLRMKPRDLVTTFGETLVELGRPILSIGLVLAFAFVANYSGMSSTLALLLAGTGAAFPFFSPFLGWLGVFLTGSDTSS
NALFCSLQNTTAHQIGVSDTLLVAANTTGGVTAKMISPQSIAVACAATGLVGKESELFRFTVKHSLLFAVIVGIITVVQA
YLLPGMIPG

Specific function: Transports glycolate across the membrane. Can also transport L-lactate and D-lactate. Seems to be driven by a proton motive force [H]

COG id: COG1620

COG function: function code C; L-lactate permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lactate permease family [H]

Homologues:

Organism=Escherichia coli, GI1789347, Length=567, Percent_Identity=70.3703703703704, Blast_Score=768, Evalue=0.0,
Organism=Escherichia coli, GI1790031, Length=568, Percent_Identity=65.3169014084507, Blast_Score=697, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003804 [H]

Pfam domain/function: PF02652 Lactate_perm [H]

EC number: NA

Molecular weight: Translated: 58981; Mature: 58981

Theoretical pI: Translated: 9.95; Mature: 9.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPWTQIYMPLGSLWLSSLAAAIPILFFFIALAVLRLKGHVAAAGTLLLSLAVAVFAYGM
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCC
PAQQALAAAGFGFAYGLWPIAWIIVTAVFLYKIVVKTGQFDVIRASVLSITDDQRLQMLL
CHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHH
IGFAFGAFLEGAAGFGAPVAITAALLVGLGFNPLYAAGLCLIANTAPVAFGAMGIPIIVA
HHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHEECCCCHHHCCCCCCEEEE
GQVTGIDAMHIGAMAGRQLPLLSLMVPFWLVFMMDGAKGVRETWPAALVTGGSFAVTQYF
CCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHCCHHEEECCCCEEEHHH
TSNHIGPELPDITSALVSLVSLAAFLKVWQPRSASQRAGGSVGGGAMAMSAFGGGGFSNG
HCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCHHHHHHHCCCCCCCCC
VQNGGNRKSPYTTAQTIRAWAPFGILTAIVTVWSLQPFKALFAGNGALAGTVLKFKVPGL
CCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHCCCHHHHHHCCCCCCEEEEEEEECCCH
DQLVLKAAPIVASPKPYEAVLKLDLLSAVGTAILLTALISAVLLRMKPRDLVTTFGETLV
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
ELGRPILSIGLVLAFAFVANYSGMSSTLALLLAGTGAAFPFFSPFLGWLGVFLTGSDTSS
HHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHEEECCCCCC
NALFCSLQNTTAHQIGVSDTLLVAANTTGGVTAKMISPQSIAVACAATGLVGKESELFRF
CEEEEEECCCCHHHCCCCCEEEEEECCCCCEEEEEECCCHHHHHHHHHCCCCCCCHHHHH
TVKHSLLFAVIVGIITVVQAYLLPGMIPG
HHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: lactate [Periplasm]; Proton [Periplasm]; glycolate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + lactate [Periplasm] = Proton [Cytoplasm] + lactate [Cytoplasm] Proton [Periplasm] + glycolate [Periplasm] = Proton [Cytoplasm] + glycolate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9278503; 11283302; 11785976 [H]