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The map label for this gene is lutB [H]

Identifier: 73539268

GI number: 73539268

Start: 2237930

End: 2239375

Strand: Reverse

Name: lutB [H]

Synonym: Reut_B5446

Alternate gene names: 73539268

Gene position: 2239375-2237930 (Counterclockwise)

Preceding gene: 73539269

Following gene: 73539267

Centisome position: 82.14

GC content: 65.63

Gene sequence:

>1446_bases
ATGAGCGAGACCACGCTGCACTTCGTCCCGACACAGGACTTCAAGGCGCGCGCCCGCGAGGCGCTCGACGATCCCAAGCT
GCGCCAGAGCTTCCGCGGCGCGATGGATTTTCTGCAGGGCAAGCGCCGGTCGCAGTTTCCGGATACCGATGAGCTGGAGC
ATCTGCGCGATATTGGCGAGGCGATCCGCCAGCACGCGCTGGCCAATTTGCCCGACCTCCTGGTGCAGCTCGAAAGCAAC
TTGACCGCTGCCGGCGTGCAGGTGCACTGGGCCGAGACCGCCGAGGAGGCCAACGCGATCATCCATCGCATCGCCGAATC
GAAGCAGGCGAAGCGAGTGATCAAGGGCAAGTCGATGGCCAGCGAGGAAATCGAGCTGAACCATTACCTCGCCGAGCGCG
GCGTGGATTGCATCGAGTCGGACATGGGCGAGTACATCGTCCAGCTTGCCGGCGAGAAGCCCTCGCACATCGTCATGCCG
GCGATCCACAAGACCAAGGGCGACATCGCCACGCTGTTCGAGCAGCATATCCCCGACACGCCCTATACCGAGGACGTCGA
CGCGCTGATCCAGACCGGCCGCCGCGCGTTGCGCCAGGCGTTCGTCGACGCCGATATCGGGCTGTCCGGCGTGAACTTCG
CCGCGGCGGACACGGGCACGCTGTGGCTCGTGGAGAACGAGGGCAACGGACGGCTGTCGACGACGGTGCCGGACGTGCAT
ATCGCCATCATGGGGATGGAGAAGGTGGTGGCGCGGCTGGAGCATATCGTGCCGCTGTCGAGCCTTCTGACGCGCTCGGC
CACGGGGCAGCCCATCACGACCTACTTCAACCTGATCTCGGGCCCGCGCCGTGAGGGCGAGCGCGATGGGCCGCGCGAGG
TGCATCTGGTGCTGCTCGACAACGGGCGCACGCAGGCCTATGCCGATGCGCAGTTGCGCGCGACGCTGCAGTGCATCCGT
TGCGGAGCCTGCATGAACCATTGCCCGGTTTATACCCGGATCGGCGGCCACGCTTATGGCACGACGTATCCGGGGCCCAT
CGGCAAGATCATCTCGCCGCATCTGCTGGGGCTGGAGGCGACGGCCGATCTCGCTACGGCGTCGAGTCTGTGCGGTGCGT
GCGGGGAAGTGTGCCCCGTGCGCATTCCGATTCCGCAATTGCTGGTGCGTCTGCGTACCGAAGCCAACCGCAATCCCGAC
GAGCAGGTCGAGCATCCGCTGCGTGGCCAGGGGGCCAAGTACAGCCGTGGCGAGCATCTGATCTGGCGCTTCTGGAGCGG
CGCGTTCTCGCATCCGAAGGCTTATCGCATGTTCCGCTGGGCGGCTACGCGGATGCGTGCGCTGACGCCGGCGAAGCAGA
TGGGATGGACGCAGCACCGGACGCCACTGACGCCGGCGAAGCGGAGCTTGTCGGATTTGCTCAAGGATAAAGGCCAGCCG
GAGTAG

Upstream 100 bases:

>100_bases
CGAAGACCTCCGATATCCAGCAAACCCTGGCTTACGGCGCGCACGGTCCGCGCCAGCTCTGGGTCGTGATCGTCGATGAC
AGCCACGCAGGGAGCCCGGC

Downstream 100 bases:

>100_bases
GAGTCCGCCTGGTTCCCCCGTCTCCCGCTCGCGGGAGAAAAAAAGAACATCGTCGCGACGGACACGGCCGCGTGGCGCCA
ACAACAACAAGGATTTCCCG

Product: Iron-sulfur cluster binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 481; Mature: 480

Protein sequence:

>481_residues
MSETTLHFVPTQDFKARAREALDDPKLRQSFRGAMDFLQGKRRSQFPDTDELEHLRDIGEAIRQHALANLPDLLVQLESN
LTAAGVQVHWAETAEEANAIIHRIAESKQAKRVIKGKSMASEEIELNHYLAERGVDCIESDMGEYIVQLAGEKPSHIVMP
AIHKTKGDIATLFEQHIPDTPYTEDVDALIQTGRRALRQAFVDADIGLSGVNFAAADTGTLWLVENEGNGRLSTTVPDVH
IAIMGMEKVVARLEHIVPLSSLLTRSATGQPITTYFNLISGPRREGERDGPREVHLVLLDNGRTQAYADAQLRATLQCIR
CGACMNHCPVYTRIGGHAYGTTYPGPIGKIISPHLLGLEATADLATASSLCGACGEVCPVRIPIPQLLVRLRTEANRNPD
EQVEHPLRGQGAKYSRGEHLIWRFWSGAFSHPKAYRMFRWAATRMRALTPAKQMGWTQHRTPLTPAKRSLSDLLKDKGQP
E

Sequences:

>Translated_481_residues
MSETTLHFVPTQDFKARAREALDDPKLRQSFRGAMDFLQGKRRSQFPDTDELEHLRDIGEAIRQHALANLPDLLVQLESN
LTAAGVQVHWAETAEEANAIIHRIAESKQAKRVIKGKSMASEEIELNHYLAERGVDCIESDMGEYIVQLAGEKPSHIVMP
AIHKTKGDIATLFEQHIPDTPYTEDVDALIQTGRRALRQAFVDADIGLSGVNFAAADTGTLWLVENEGNGRLSTTVPDVH
IAIMGMEKVVARLEHIVPLSSLLTRSATGQPITTYFNLISGPRREGERDGPREVHLVLLDNGRTQAYADAQLRATLQCIR
CGACMNHCPVYTRIGGHAYGTTYPGPIGKIISPHLLGLEATADLATASSLCGACGEVCPVRIPIPQLLVRLRTEANRNPD
EQVEHPLRGQGAKYSRGEHLIWRFWSGAFSHPKAYRMFRWAATRMRALTPAKQMGWTQHRTPLTPAKRSLSDLLKDKGQP
E
>Mature_480_residues
SETTLHFVPTQDFKARAREALDDPKLRQSFRGAMDFLQGKRRSQFPDTDELEHLRDIGEAIRQHALANLPDLLVQLESNL
TAAGVQVHWAETAEEANAIIHRIAESKQAKRVIKGKSMASEEIELNHYLAERGVDCIESDMGEYIVQLAGEKPSHIVMPA
IHKTKGDIATLFEQHIPDTPYTEDVDALIQTGRRALRQAFVDADIGLSGVNFAAADTGTLWLVENEGNGRLSTTVPDVHI
AIMGMEKVVARLEHIVPLSSLLTRSATGQPITTYFNLISGPRREGERDGPREVHLVLLDNGRTQAYADAQLRATLQCIRC
GACMNHCPVYTRIGGHAYGTTYPGPIGKIISPHLLGLEATADLATASSLCGACGEVCPVRIPIPQLLVRLRTEANRNPDE
QVEHPLRGQGAKYSRGEHLIWRFWSGAFSHPKAYRMFRWAATRMRALTPAKQMGWTQHRTPLTPAKRSLSDLLKDKGQPE

Specific function: Is essential for L-lactate degradation and allows cells to grow with lactate as the sole carbon source. Has probably a role as an electron transporter during oxidation of L-lactate. May also allow cells to utilize an alternative carbon source during biofi

COG id: COG1139

COG function: function code C; Uncharacterized conserved protein containing a ferredoxin-like domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1786498, Length=384, Percent_Identity=44.53125, Blast_Score=348, Evalue=4e-97,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017900
- InterPro:   IPR003741
- InterPro:   IPR004452
- InterPro:   IPR002698
- InterPro:   IPR012285
- InterPro:   IPR009051
- InterPro:   IPR022825 [H]

Pfam domain/function: PF02589 DUF162 [H]

EC number: NA

Molecular weight: Translated: 53310; Mature: 53179

Theoretical pI: Translated: 7.31; Mature: 7.31

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSETTLHFVPTQDFKARAREALDDPKLRQSFRGAMDFLQGKRRSQFPDTDELEHLRDIGE
CCCCEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
AIRQHALANLPDLLVQLESNLTAAGVQVHWAETAEEANAIIHRIAESKQAKRVIKGKSMA
HHHHHHHHCHHHHHHHHHCCCEECCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SEEIELNHYLAERGVDCIESDMGEYIVQLAGEKPSHIVMPAIHKTKGDIATLFEQHIPDT
HHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCEEEECCHHCCCCHHHHHHHHHCCCC
PYTEDVDALIQTGRRALRQAFVDADIGLSGVNFAAADTGTLWLVENEGNGRLSTTVPDVH
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCEEEEEEECCCCEEEECCCCEE
IAIMGMEKVVARLEHIVPLSSLLTRSATGQPITTYFNLISGPRREGERDGPREVHLVLLD
EEEECHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCEEEEEEEE
NGRTQAYADAQLRATLQCIRCGACMNHCPVYTRIGGHAYGTTYPGPIGKIISPHLLGLEA
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCHHHCCCHHCCCCH
TADLATASSLCGACGEVCPVRIPIPQLLVRLRTEANRNPDEQVEHPLRGQGAKYSRGEHL
HHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCCEE
IWRFWSGAFSHPKAYRMFRWAATRMRALTPAKQMGWTQHRTPLTPAKRSLSDLLKDKGQP
EEEECCCCCCCCHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCHHHHHHHHHHHCCCCC
E
C
>Mature Secondary Structure 
SETTLHFVPTQDFKARAREALDDPKLRQSFRGAMDFLQGKRRSQFPDTDELEHLRDIGE
CCCEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
AIRQHALANLPDLLVQLESNLTAAGVQVHWAETAEEANAIIHRIAESKQAKRVIKGKSMA
HHHHHHHHCHHHHHHHHHCCCEECCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SEEIELNHYLAERGVDCIESDMGEYIVQLAGEKPSHIVMPAIHKTKGDIATLFEQHIPDT
HHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCEEEECCHHCCCCHHHHHHHHHCCCC
PYTEDVDALIQTGRRALRQAFVDADIGLSGVNFAAADTGTLWLVENEGNGRLSTTVPDVH
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCEEEEEEECCCCEEEECCCCEE
IAIMGMEKVVARLEHIVPLSSLLTRSATGQPITTYFNLISGPRREGERDGPREVHLVLLD
EEEECHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCEEEEEEEE
NGRTQAYADAQLRATLQCIRCGACMNHCPVYTRIGGHAYGTTYPGPIGKIISPHLLGLEA
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCHHHCCCHHCCCCH
TADLATASSLCGACGEVCPVRIPIPQLLVRLRTEANRNPDEQVEHPLRGQGAKYSRGEHL
HHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCCEE
IWRFWSGAFSHPKAYRMFRWAATRMRALTPAKQMGWTQHRTPLTPAKRSLSDLLKDKGQP
EEEECCCCCCCCHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCHHHHHHHHHHHCCCCC
E
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]