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The map label for this gene is prfC [H]

Identifier: 73539156

GI number: 73539156

Start: 2101276

End: 2102877

Strand: Reverse

Name: prfC [H]

Synonym: Reut_B5334

Alternate gene names: 73539156

Gene position: 2102877-2101276 (Counterclockwise)

Preceding gene: 73539157

Following gene: 73539155

Centisome position: 77.14

GC content: 64.48

Gene sequence:

>1602_bases
GTGAGCTCGCTCGTATCCGAAATTGCGCGTCGACGCACCTTTGCCATCATCTCCCACCCCGACGCGGGCAAGACCACTCT
GACCGAAAAGCTGCTGTGGTTCGGCGGCGCGATCCAGGTGGCCGGCGAAGTGCGCGCGCGCAAGGCTGACCGCCATGCCA
CGTCGGACTGGATGGAGCTGGAAAAGCAGCGCGGCATTTCGGTGACCTCGTCGGTGATGCAGTTCCCGTACCGCCGCGAA
GCTGCCGACGGCAAGGCCGAGGAGAACATCGTCAACCTGCTCGACACGCCGGGCCACGAGGATTTCTCCGAAGATACCTA
CCGCACGCTGACTGCCGTGGACTCGGCCGTGATGGTGATCGACTCGGTCAATGGCGTGGAAGCGCAGACCATCAAGCTGC
TCAACGTCTGCCGCCTGCGCGCTACGCCGATCCTCACCTTCATCAACAAGCTCGACCGCGAAGGCCGTTCGCCGATCGAG
TTGCTCGACGAAATCGAAGACGTGCTGCAGATTCAGTGCGCGCCGATGACCTGGCCGATCGGCATGGGCAAGGCCTTCCG
GGGCGTGTACCACCTGATCGACGACAAGGTGCAGCTGTTCGATCCGCACGGTGACAAGGGTACGGCGGCCATTCTCGATG
GCCTCGACAACCCGGAGCTTGACCGCATCCTTGGCAGCCAGGCCGACGAGCTGCGCCTCGAGATCGAACTGGTGCGCGGT
GCCTCGCACACGTTCGACAAGGAAGCGTTCCTGGCCGGCAAGCAGACGCCGGTCTACTTCGGCTCCGCCATCAACAACTT
CGGTGTGCAATCTCTGCTCGACGCGCTGTGCCAGCTGTCGCCCCCGCCGCTCGCGCGCAATACCGAGTCGCGCGTGGTCG
AGCCGCAGGAAGGCAAGTTCACGGGTTTCGTGTTCAAGATCCAGGCCAATATGGATCCGCGCCACCGCGACCGCATTGCC
TTCGTGCGCGTGTGCTCGGGCCGCTTCGAGCGCGGCATGAAGCTGCTGCACGTGTCGCAGGCCAAGACCGTGGCGATCAA
CAACGCCATCACGTTCATGGCGCAGGACCGCAACACGACGGAAGAGGCCTACGCGGGCGACATCATCGGCGTGCCGAACC
ACGGCACCATCCGGCTGGGCGATGTCTTCACCGAGGGTGAGCCGCTGAAGTTCACGGGCATCCCGTCGTTCGCGCCCGAG
TTCTTCCGCCGCGCGCGACTGAACAACCCGCTCAAGGTCAAGCAACTGCAGAAGGGCCTGCAGCAGCTCGCGGAAGAGGG
CGCCACGCAGATGTTCCGCCCGCTCGCGTCGAACGAACTCGTGCTTGGCGCGGTCGGTATCCTGCAGTTCGACGTGGTGG
CGCACCGGCTCGAGCACGAGTACGGCGTCGATGCCATTTTCGAGTCGCACGAATGCGCGACCGCGCGCTGGCTCAAGGGC
GCGCCGGCCGAGATCGAAAAGCTCATTGCCAAGGCCGGCCACAACGTGGCGCTCGACGGCGCCGGCGACCATGTGTACCT
GGCGCCCAGCATGGTCAACCTGCGCCTCACCCAGGAAAAATTCCCGGACGTGCAGTTCCTGGAAACGCGCGAGATCGTCT
GA

Upstream 100 bases:

>100_bases
CCCGCAGTGCCGGCTCGACCGCCACCCTGCGGTCCTCGTCGTTGCCCGGTCGGCTCCCGCATGCCGGGCCCTGCAATCTA
CCCGATCCCTATCCGCTGCC

Downstream 100 bases:

>100_bases
CCCGAGCGCATCCCGGATGCAAAACGGCCCGCAGCAGTGCGGGCCGTTTTGCATGGAAGTGCCGTTTTGCGCGCATTGGC
GCCTGGGATAGCAACGCGGC

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 533; Mature: 532

Protein sequence:

>533_residues
MSSLVSEIARRRTFAIISHPDAGKTTLTEKLLWFGGAIQVAGEVRARKADRHATSDWMELEKQRGISVTSSVMQFPYRRE
AADGKAEENIVNLLDTPGHEDFSEDTYRTLTAVDSAVMVIDSVNGVEAQTIKLLNVCRLRATPILTFINKLDREGRSPIE
LLDEIEDVLQIQCAPMTWPIGMGKAFRGVYHLIDDKVQLFDPHGDKGTAAILDGLDNPELDRILGSQADELRLEIELVRG
ASHTFDKEAFLAGKQTPVYFGSAINNFGVQSLLDALCQLSPPPLARNTESRVVEPQEGKFTGFVFKIQANMDPRHRDRIA
FVRVCSGRFERGMKLLHVSQAKTVAINNAITFMAQDRNTTEEAYAGDIIGVPNHGTIRLGDVFTEGEPLKFTGIPSFAPE
FFRRARLNNPLKVKQLQKGLQQLAEEGATQMFRPLASNELVLGAVGILQFDVVAHRLEHEYGVDAIFESHECATARWLKG
APAEIEKLIAKAGHNVALDGAGDHVYLAPSMVNLRLTQEKFPDVQFLETREIV

Sequences:

>Translated_533_residues
MSSLVSEIARRRTFAIISHPDAGKTTLTEKLLWFGGAIQVAGEVRARKADRHATSDWMELEKQRGISVTSSVMQFPYRRE
AADGKAEENIVNLLDTPGHEDFSEDTYRTLTAVDSAVMVIDSVNGVEAQTIKLLNVCRLRATPILTFINKLDREGRSPIE
LLDEIEDVLQIQCAPMTWPIGMGKAFRGVYHLIDDKVQLFDPHGDKGTAAILDGLDNPELDRILGSQADELRLEIELVRG
ASHTFDKEAFLAGKQTPVYFGSAINNFGVQSLLDALCQLSPPPLARNTESRVVEPQEGKFTGFVFKIQANMDPRHRDRIA
FVRVCSGRFERGMKLLHVSQAKTVAINNAITFMAQDRNTTEEAYAGDIIGVPNHGTIRLGDVFTEGEPLKFTGIPSFAPE
FFRRARLNNPLKVKQLQKGLQQLAEEGATQMFRPLASNELVLGAVGILQFDVVAHRLEHEYGVDAIFESHECATARWLKG
APAEIEKLIAKAGHNVALDGAGDHVYLAPSMVNLRLTQEKFPDVQFLETREIV
>Mature_532_residues
SSLVSEIARRRTFAIISHPDAGKTTLTEKLLWFGGAIQVAGEVRARKADRHATSDWMELEKQRGISVTSSVMQFPYRREA
ADGKAEENIVNLLDTPGHEDFSEDTYRTLTAVDSAVMVIDSVNGVEAQTIKLLNVCRLRATPILTFINKLDREGRSPIEL
LDEIEDVLQIQCAPMTWPIGMGKAFRGVYHLIDDKVQLFDPHGDKGTAAILDGLDNPELDRILGSQADELRLEIELVRGA
SHTFDKEAFLAGKQTPVYFGSAINNFGVQSLLDALCQLSPPPLARNTESRVVEPQEGKFTGFVFKIQANMDPRHRDRIAF
VRVCSGRFERGMKLLHVSQAKTVAINNAITFMAQDRNTTEEAYAGDIIGVPNHGTIRLGDVFTEGEPLKFTGIPSFAPEF
FRRARLNNPLKVKQLQKGLQQLAEEGATQMFRPLASNELVLGAVGILQFDVVAHRLEHEYGVDAIFESHECATARWLKGA
PAEIEKLIAKAGHNVALDGAGDHVYLAPSMVNLRLTQEKFPDVQFLETREIV

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=495, Percent_Identity=29.0909090909091, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI19923640, Length=513, Percent_Identity=26.3157894736842, Blast_Score=139, Evalue=9e-33,
Organism=Homo sapiens, GI25306283, Length=384, Percent_Identity=28.125, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI25306287, Length=482, Percent_Identity=26.5560165975104, Blast_Score=125, Evalue=9e-29,
Organism=Homo sapiens, GI157426893, Length=173, Percent_Identity=29.4797687861272, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI94966754, Length=268, Percent_Identity=26.4925373134328, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI4503483, Length=165, Percent_Identity=31.5151515151515, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI310132016, Length=131, Percent_Identity=35.8778625954198, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI310110807, Length=131, Percent_Identity=35.8778625954198, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI310123363, Length=131, Percent_Identity=35.8778625954198, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1790835, Length=537, Percent_Identity=60.8938547486033, Blast_Score=669, Evalue=0.0,
Organism=Escherichia coli, GI1789738, Length=495, Percent_Identity=27.8787878787879, Blast_Score=158, Evalue=1e-39,
Organism=Escherichia coli, GI1788922, Length=160, Percent_Identity=34.375, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI48994988, Length=191, Percent_Identity=30.8900523560209, Blast_Score=86, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17533571, Length=502, Percent_Identity=27.2908366533865, Blast_Score=158, Evalue=7e-39,
Organism=Caenorhabditis elegans, GI17556745, Length=486, Percent_Identity=23.2510288065844, Blast_Score=111, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI17557151, Length=144, Percent_Identity=31.25, Blast_Score=77, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17506493, Length=162, Percent_Identity=32.0987654320988, Blast_Score=75, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6323098, Length=484, Percent_Identity=27.8925619834711, Blast_Score=173, Evalue=6e-44,
Organism=Saccharomyces cerevisiae, GI6322359, Length=437, Percent_Identity=26.5446224256293, Blast_Score=127, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6324707, Length=178, Percent_Identity=32.5842696629214, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6320593, Length=178, Percent_Identity=32.5842696629214, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6323320, Length=142, Percent_Identity=28.8732394366197, Blast_Score=74, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6324166, Length=149, Percent_Identity=34.2281879194631, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24582462, Length=521, Percent_Identity=29.3666026871401, Blast_Score=177, Evalue=2e-44,
Organism=Drosophila melanogaster, GI221458488, Length=552, Percent_Identity=25, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI78706572, Length=159, Percent_Identity=33.3333333333333, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24585709, Length=154, Percent_Identity=32.4675324675325, Blast_Score=75, Evalue=9e-14,
Organism=Drosophila melanogaster, GI24585711, Length=154, Percent_Identity=32.4675324675325, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24585713, Length=154, Percent_Identity=32.4675324675325, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: NA

Molecular weight: Translated: 59125; Mature: 58994

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSLVSEIARRRTFAIISHPDAGKTTLTEKLLWFGGAIQVAGEVRARKADRHATSDWMEL
CHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHCCCEEEHHHHHHHHHHCCCCHHHHHH
EKQRGISVTSSVMQFPYRREAADGKAEENIVNLLDTPGHEDFSEDTYRTLTAVDSAVMVI
HHHCCCHHHHHHHHCCCHHHCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHEEEEE
DSVNGVEAQTIKLLNVCRLRATPILTFINKLDREGRSPIELLDEIEDVLQIQCAPMTWPI
ECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHEEEECCCCCCC
GMGKAFRGVYHLIDDKVQLFDPHGDKGTAAILDGLDNPELDRILGSQADELRLEIELVRG
CCCHHHHHHHHHHCCCEEEECCCCCCCCCHHHCCCCCCHHHHHHCCCCHHEEEEEEEEEC
ASHTFDKEAFLAGKQTPVYFGSAINNFGVQSLLDALCQLSPPPLARNTESRVVEPQEGKF
CCCCCCCHHHHCCCCCCEEECCHHHHCCHHHHHHHHHCCCCCCCCCCCHHCCCCCCCCCE
TGFVFKIQANMDPRHRDRIAFVRVCSGRFERGMKLLHVSQAKTVAINNAITFMAQDRNTT
EEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEECCEEEEEECCCCCC
EEAYAGDIIGVPNHGTIRLGDVFTEGEPLKFTGIPSFAPEFFRRARLNNPLKVKQLQKGL
HHHHCCCEEECCCCCEEEECCEECCCCCEEECCCCCHHHHHHHHHHCCCCCHHHHHHHHH
QQLAEEGATQMFRPLASNELVLGAVGILQFDVVAHRLEHEYGVDAIFESHECATARWLKG
HHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHCC
APAEIEKLIAKAGHNVALDGAGDHVYLAPSMVNLRLTQEKFPDVQFLETREIV
CHHHHHHHHHHCCCCEEEECCCCEEEECCCEEEEEECHHHCCCCEEHHCCCCC
>Mature Secondary Structure 
SSLVSEIARRRTFAIISHPDAGKTTLTEKLLWFGGAIQVAGEVRARKADRHATSDWMEL
HHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHCCCEEEHHHHHHHHHHCCCCHHHHHH
EKQRGISVTSSVMQFPYRREAADGKAEENIVNLLDTPGHEDFSEDTYRTLTAVDSAVMVI
HHHCCCHHHHHHHHCCCHHHCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHEEEEE
DSVNGVEAQTIKLLNVCRLRATPILTFINKLDREGRSPIELLDEIEDVLQIQCAPMTWPI
ECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHEEEECCCCCCC
GMGKAFRGVYHLIDDKVQLFDPHGDKGTAAILDGLDNPELDRILGSQADELRLEIELVRG
CCCHHHHHHHHHHCCCEEEECCCCCCCCCHHHCCCCCCHHHHHHCCCCHHEEEEEEEEEC
ASHTFDKEAFLAGKQTPVYFGSAINNFGVQSLLDALCQLSPPPLARNTESRVVEPQEGKF
CCCCCCCHHHHCCCCCCEEECCHHHHCCHHHHHHHHHCCCCCCCCCCCHHCCCCCCCCCE
TGFVFKIQANMDPRHRDRIAFVRVCSGRFERGMKLLHVSQAKTVAINNAITFMAQDRNTT
EEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEECCEEEEEECCCCCC
EEAYAGDIIGVPNHGTIRLGDVFTEGEPLKFTGIPSFAPEFFRRARLNNPLKVKQLQKGL
HHHHCCCEEECCCCCEEEECCEECCCCCEEECCCCCHHHHHHHHHHCCCCCHHHHHHHHH
QQLAEEGATQMFRPLASNELVLGAVGILQFDVVAHRLEHEYGVDAIFESHECATARWLKG
HHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHCC
APAEIEKLIAKAGHNVALDGAGDHVYLAPSMVNLRLTQEKFPDVQFLETREIV
CHHHHHHHHHHCCCCEEEECCCCEEEECCCEEEEEECHHHCCCCEEHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA