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The map label for this gene is leuD [H]

Identifier: 73538667

GI number: 73538667

Start: 1549956

End: 1550498

Strand: Reverse

Name: leuD [H]

Synonym: Reut_B4842

Alternate gene names: 73538667

Gene position: 1550498-1549956 (Counterclockwise)

Preceding gene: 73538668

Following gene: 73538666

Centisome position: 56.87

GC content: 66.48

Gene sequence:

>543_bases
ATGAACGACACGCTCATGCCCGCCACAGGGCACTTCACCGGTCGCGCCTGGGTGTTCGGCGACGATATCAATACCGACCT
GCTGGCGCCCGGCGCGTATATGAAGTACGGCATCGATGCGCTCGCGCGCCATTGCATGGAGCATGTCGATCCCGGGTTTG
CCGCCGCGGTGCGGCCCGGAGACATCGTGTTCGGCGGGCGCAATTTCGGCGCGGGCTCGTCGCGCGAACAGGCGGTGGAG
GTCTTGCGGCATCTGGGCGTGGCAGCCGTAGTCGCGCCGTCGTTCGCCGGGCTGTTCTATCGCAACGGCTTCAACCTGGG
CCTCCCGCTGTTCACTTGCCCGTCGCTGCCGCGTATTGCGGCGGGCCAGCGCGCCGGTTGCGATCTTGCGCGTGCGCAGG
TCTTTGTCGAAGGCCTCGCGCCACTACAATGCGAACCGGTTCCGCCGCACCTGGTGGACATGATCCAAGACGGCGGCCTG
GTGCCGCACCTCGCCCGCAAGATCGCGGCGGGCCAACTTTCCGTGAACGGAAATCCAGCATGA

Upstream 100 bases:

>100_bases
GCATGGGCGCCGCATCGTCGCAGGTTTATCTCGGTTCGCCCTACACGGTGGCCGCGTCTGCAATTGCCGGCCGAATCGCC
GATCCACGGGGGATGCTGTC

Downstream 100 bases:

>100_bases
CACTGAAGCAACGCCTGCAACAACCTGGCATCGTGACCGCACCGGGTGTCTATGACGCCTTTTCCGCACTGCTGGTCGAA
CAGGCCGGGTTCCAGGCTGC

Product: 3-isopropylmalate dehydratase, small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 180; Mature: 180

Protein sequence:

>180_residues
MNDTLMPATGHFTGRAWVFGDDINTDLLAPGAYMKYGIDALARHCMEHVDPGFAAAVRPGDIVFGGRNFGAGSSREQAVE
VLRHLGVAAVVAPSFAGLFYRNGFNLGLPLFTCPSLPRIAAGQRAGCDLARAQVFVEGLAPLQCEPVPPHLVDMIQDGGL
VPHLARKIAAGQLSVNGNPA

Sequences:

>Translated_180_residues
MNDTLMPATGHFTGRAWVFGDDINTDLLAPGAYMKYGIDALARHCMEHVDPGFAAAVRPGDIVFGGRNFGAGSSREQAVE
VLRHLGVAAVVAPSFAGLFYRNGFNLGLPLFTCPSLPRIAAGQRAGCDLARAQVFVEGLAPLQCEPVPPHLVDMIQDGGL
VPHLARKIAAGQLSVNGNPA
>Mature_180_residues
MNDTLMPATGHFTGRAWVFGDDINTDLLAPGAYMKYGIDALARHCMEHVDPGFAAAVRPGDIVFGGRNFGAGSSREQAVE
VLRHLGVAAVVAPSFAGLFYRNGFNLGLPLFTCPSLPRIAAGQRAGCDLARAQVFVEGLAPLQCEPVPPHLVDMIQDGGL
VPHLARKIAAGQLSVNGNPA

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 2 subfamily [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6320440, Length=101, Percent_Identity=45.5445544554455, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- InterPro:   IPR011827 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 18931; Mature: 18931

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDTLMPATGHFTGRAWVFGDDINTDLLAPGAYMKYGIDALARHCMEHVDPGFAAAVRPG
CCCCCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCC
DIVFGGRNFGAGSSREQAVEVLRHLGVAAVVAPSFAGLFYRNGFNLGLPLFTCPSLPRIA
CEEECCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCEECCCCCCCC
AGQRAGCDLARAQVFVEGLAPLQCEPVPPHLVDMIQDGGLVPHLARKIAAGQLSVNGNPA
CCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHCEEECCCCCC
>Mature Secondary Structure
MNDTLMPATGHFTGRAWVFGDDINTDLLAPGAYMKYGIDALARHCMEHVDPGFAAAVRPG
CCCCCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCC
DIVFGGRNFGAGSSREQAVEVLRHLGVAAVVAPSFAGLFYRNGFNLGLPLFTCPSLPRIA
CEEECCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCEECCCCCCCC
AGQRAGCDLARAQVFVEGLAPLQCEPVPPHLVDMIQDGGLVPHLARKIAAGQLSVNGNPA
CCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA