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The map label for this gene is leuC2 [H]

Identifier: 73538668

GI number: 73538668

Start: 1550495

End: 1551802

Strand: Reverse

Name: leuC2 [H]

Synonym: Reut_B4843

Alternate gene names: 73538668

Gene position: 1551802-1550495 (Counterclockwise)

Preceding gene: 73538669

Following gene: 73538667

Centisome position: 56.92

GC content: 68.81

Gene sequence:

>1308_bases
ATGGCGCAGGTGTCCGCACAGGCCGTCGCAGCGGCCACGCCCCAGACGCTCGCCCAGAAACTGCTGGCCCGCGCATCCGG
CCGCGCGCATGTCACGCCGGGCGAGATCGTCACCTGCCGTGTCGATCTGGCCATGATGCATGATTCGGGTGGCCCGCGGC
GCGTCAAGCCGCTGCTGGAGAAGATCGGCGCCCGGGTCTGGGACCCGTCCAGGGTGGTGGTGGTCACAGACCACTATGTG
CCGGCCCATGACGCCGACAGCCGCAAGATCGTGCAGATCGCGCGCGAGTGGGTCAGGGAGGAGGGCGTGGCGCGCTTCCA
TGACATGGAGGGCATCTGCCATGTGGTGGTGCCGCAGAAGGGCTACCTGCGTCCGGGCATGATGGCGGTGGGTGGTGACA
GCCACTCCTCAACGGGTGGGGCCTTCGGCACATACATGTTCGGCATCGGGGCGACAGAGATGCTCGGCGTACTGGTGACC
GGCGAGATCTGGGTGCGCGTACCGCAGACCCTCCGCATGCAATGGCACGGCCGGCTGCGCCCCGGCGTGTCGGCAAAGGA
CATGATGCTGCGCATGTGCACGCAATTCGGCATGGACGGGGGCCAGTATCAGGCCGTTGAATATGCCGGCACCGCCGTGT
CGGCGTTGTCGATGCAGGAGCGCATGACGCTGTCGAACATGACCGCGGAGCTGGGCGCCCAGGTCGGCCTGGTGGCGCCC
GACGCGGTGACGCGCGACTGGCTGCTCGACGCCGGCGTGGAGCCATCGGAGATTGACGTCGACCGCTGGCAGTCGGACGC
CGGCGCCGCATGCCTGGCTACGCACGACTTCGACGCTGCGTCGCTGGCGCCGCAAGTGTCGCGGCCGCATACCCCGGCCG
ATGCCAGCGACGTCGACAGCCTGGGCAATGTGGCGGTCAATATCGCATACATCGGTGCCTGCACCGGCGCCAAGCTCGAC
GACCTGCGCATGGCCGCGCAAGTGCTGCGCGGACAGCGCGTGGCGCGCAATAGCCGGCTGCTGGTGGCGCCGGCCAGCGC
CGCGGACCAGGCGCAGGCCGAGCGCGAGGGCACGCTGGGCGCGCTGGTGGCGGCCGGCGCCGAGCTTCTGCCAAACGCTT
GCGGCGCGTGCGCTGGCTATGGCGAGCACCGCTTTGGCGAGGACACCGTGGTGATCAGTACCACTGCGCGCAATTTCAAG
GGACGCATGGGCGCCGCATCGTCGCAGGTTTATCTCGGTTCGCCCTACACGGTGGCCGCGTCTGCAATTGCCGGCCGAAT
CGCCGATCCACGGGGGATGCTGTCATGA

Upstream 100 bases:

>100_bases
GCGCGGTGAGCATGACGCCGCAGGCGTTCGCGCAGTTTGTCCGCAGCGATATCCAGCACTGGGCCAAGGTGATCCAGGCA
TCGGGCACACGGGTGGAATG

Downstream 100 bases:

>100_bases
ACGACACGCTCATGCCCGCCACAGGGCACTTCACCGGTCGCGCCTGGGTGTTCGGCGACGATATCAATACCGACCTGCTG
GCGCCCGGCGCGTATATGAA

Product: 3-isopropylmalate dehydratase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase 2; IPMI 2; Isopropylmalate isomerase 2 [H]

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MAQVSAQAVAAATPQTLAQKLLARASGRAHVTPGEIVTCRVDLAMMHDSGGPRRVKPLLEKIGARVWDPSRVVVVTDHYV
PAHDADSRKIVQIAREWVREEGVARFHDMEGICHVVVPQKGYLRPGMMAVGGDSHSSTGGAFGTYMFGIGATEMLGVLVT
GEIWVRVPQTLRMQWHGRLRPGVSAKDMMLRMCTQFGMDGGQYQAVEYAGTAVSALSMQERMTLSNMTAELGAQVGLVAP
DAVTRDWLLDAGVEPSEIDVDRWQSDAGAACLATHDFDAASLAPQVSRPHTPADASDVDSLGNVAVNIAYIGACTGAKLD
DLRMAAQVLRGQRVARNSRLLVAPASAADQAQAEREGTLGALVAAGAELLPNACGACAGYGEHRFGEDTVVISTTARNFK
GRMGAASSQVYLGSPYTVAASAIAGRIADPRGMLS

Sequences:

>Translated_435_residues
MAQVSAQAVAAATPQTLAQKLLARASGRAHVTPGEIVTCRVDLAMMHDSGGPRRVKPLLEKIGARVWDPSRVVVVTDHYV
PAHDADSRKIVQIAREWVREEGVARFHDMEGICHVVVPQKGYLRPGMMAVGGDSHSSTGGAFGTYMFGIGATEMLGVLVT
GEIWVRVPQTLRMQWHGRLRPGVSAKDMMLRMCTQFGMDGGQYQAVEYAGTAVSALSMQERMTLSNMTAELGAQVGLVAP
DAVTRDWLLDAGVEPSEIDVDRWQSDAGAACLATHDFDAASLAPQVSRPHTPADASDVDSLGNVAVNIAYIGACTGAKLD
DLRMAAQVLRGQRVARNSRLLVAPASAADQAQAEREGTLGALVAAGAELLPNACGACAGYGEHRFGEDTVVISTTARNFK
GRMGAASSQVYLGSPYTVAASAIAGRIADPRGMLS
>Mature_434_residues
AQVSAQAVAAATPQTLAQKLLARASGRAHVTPGEIVTCRVDLAMMHDSGGPRRVKPLLEKIGARVWDPSRVVVVTDHYVP
AHDADSRKIVQIAREWVREEGVARFHDMEGICHVVVPQKGYLRPGMMAVGGDSHSSTGGAFGTYMFGIGATEMLGVLVTG
EIWVRVPQTLRMQWHGRLRPGVSAKDMMLRMCTQFGMDGGQYQAVEYAGTAVSALSMQERMTLSNMTAELGAQVGLVAPD
AVTRDWLLDAGVEPSEIDVDRWQSDAGAACLATHDFDAASLAPQVSRPHTPADASDVDSLGNVAVNIAYIGACTGAKLDD
LRMAAQVLRGQRVARNSRLLVAPASAADQAQAEREGTLGALVAAGAELLPNACGACAGYGEHRFGEDTVVISTTARNFKG
RMGAASSQVYLGSPYTVAASAIAGRIADPRGMLS

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=381, Percent_Identity=27.2965879265092, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI8659555, Length=484, Percent_Identity=23.3471074380165, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI41352693, Length=395, Percent_Identity=24.0506329113924, Blast_Score=83, Evalue=4e-16,
Organism=Escherichia coli, GI1786259, Length=470, Percent_Identity=31.7021276595745, Blast_Score=202, Evalue=2e-53,
Organism=Escherichia coli, GI2367097, Length=443, Percent_Identity=20.9932279909707, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787531, Length=356, Percent_Identity=23.5955056179775, Blast_Score=66, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI32564738, Length=345, Percent_Identity=28.1159420289855, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI25149337, Length=345, Percent_Identity=28.1159420289855, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI25149342, Length=282, Percent_Identity=28.0141843971631, Blast_Score=108, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17568399, Length=389, Percent_Identity=24.6786632390745, Blast_Score=82, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6321429, Length=446, Percent_Identity=31.1659192825112, Blast_Score=187, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6320440, Length=430, Percent_Identity=30.6976744186047, Blast_Score=181, Evalue=3e-46,
Organism=Saccharomyces cerevisiae, GI6323335, Length=338, Percent_Identity=27.810650887574, Blast_Score=127, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6322261, Length=371, Percent_Identity=24.5283018867925, Blast_Score=112, Evalue=1e-25,
Organism=Drosophila melanogaster, GI28571643, Length=336, Percent_Identity=26.4880952380952, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI281365315, Length=337, Percent_Identity=25.5192878338279, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI17864292, Length=337, Percent_Identity=25.5192878338279, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI161076999, Length=337, Percent_Identity=25.5192878338279, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI24645686, Length=368, Percent_Identity=23.9130434782609, Blast_Score=75, Evalue=8e-14,
Organism=Drosophila melanogaster, GI17137564, Length=369, Percent_Identity=23.8482384823848, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR011826
- InterPro:   IPR015936
- InterPro:   IPR006251 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 45936; Mature: 45805

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQVSAQAVAAATPQTLAQKLLARASGRAHVTPGEIVTCRVDLAMMHDSGGPRRVKPLLE
CCCCCCHHHHHCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEEEECCCCCHHHHHHHH
KIGARVWDPSRVVVVTDHYVPAHDADSRKIVQIAREWVREEGVARFHDMEGICHVVVPQK
HHCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHCCCCCEEEEECCC
GYLRPGMMAVGGDSHSSTGGAFGTYMFGIGATEMLGVLVTGEIWVRVPQTLRMQWHGRLR
CCCCCCCEEECCCCCCCCCCCHHHHHHHCCHHHHHHEEEECCEEEECCHHHHHHHCCCCC
PGVSAKDMMLRMCTQFGMDGGQYQAVEYAGTAVSALSMQERMTLSNMTAELGAQVGLVAP
CCCCHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECC
DAVTRDWLLDAGVEPSEIDVDRWQSDAGAACLATHDFDAASLAPQVSRPHTPADASDVDS
CHHHHHHHHHCCCCCCCCCHHHHCCCCCCEEEEECCCCHHHCCCCCCCCCCCCCCHHHHH
LGNVAVNIAYIGACTGAKLDDLRMAAQVLRGQRVARNSRLLVAPASAADQAQAEREGTLG
HCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHCCCHH
ALVAAGAELLPNACGACAGYGEHRFGEDTVVISTTARNFKGRMGAASSQVYLGSPYTVAA
HHHHHCHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCEEEECCCHHHHH
SAIAGRIADPRGMLS
HHHHHCCCCCCCCCC
>Mature Secondary Structure 
AQVSAQAVAAATPQTLAQKLLARASGRAHVTPGEIVTCRVDLAMMHDSGGPRRVKPLLE
CCCCCHHHHHCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEEEECCCCCHHHHHHHH
KIGARVWDPSRVVVVTDHYVPAHDADSRKIVQIAREWVREEGVARFHDMEGICHVVVPQK
HHCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHCCCCCEEEEECCC
GYLRPGMMAVGGDSHSSTGGAFGTYMFGIGATEMLGVLVTGEIWVRVPQTLRMQWHGRLR
CCCCCCCEEECCCCCCCCCCCHHHHHHHCCHHHHHHEEEECCEEEECCHHHHHHHCCCCC
PGVSAKDMMLRMCTQFGMDGGQYQAVEYAGTAVSALSMQERMTLSNMTAELGAQVGLVAP
CCCCHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECC
DAVTRDWLLDAGVEPSEIDVDRWQSDAGAACLATHDFDAASLAPQVSRPHTPADASDVDS
CHHHHHHHHHCCCCCCCCCHHHHCCCCCCEEEEECCCCHHHCCCCCCCCCCCCCCHHHHH
LGNVAVNIAYIGACTGAKLDDLRMAAQVLRGQRVARNSRLLVAPASAADQAQAEREGTLG
HCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHCCCHH
ALVAAGAELLPNACGACAGYGEHRFGEDTVVISTTARNFKGRMGAASSQVYLGSPYTVAA
HHHHHCHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCEEEECCCHHHHH
SAIAGRIADPRGMLS
HHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10567266 [H]