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The map label for this gene is prpB [H]

Identifier: 73538666

GI number: 73538666

Start: 1549105

End: 1549959

Strand: Reverse

Name: prpB [H]

Synonym: Reut_B4841

Alternate gene names: 73538666

Gene position: 1549959-1549105 (Counterclockwise)

Preceding gene: 73538667

Following gene: 73538665

Centisome position: 56.86

GC content: 66.2

Gene sequence:

>855_bases
ATGACACTGAAGCAACGCCTGCAACAACCTGGCATCGTGACCGCACCGGGTGTCTATGACGCCTTTTCCGCACTGCTGGT
CGAACAGGCCGGGTTCCAGGCTGCCTATCTTTCCGGTGCCAGCCTTGCCTACACGCGCTTTGGCCGGCCGGATATCGGCT
TACTGTCGCTTGATGACGTGGCTTCTGTCACCCGCAATATCCGCGAGCGCGTGGCGCTGCCGCTGATCGTCGATGCCGAC
ACCGGCTTCGGCAATGCCCTCAACGTCGTGCAGACCGTACGCGTGCTCGAGCGTGCCGGCGCATCGGCGATCCAGCTGGA
AGACCAGGCGATGCCGAAGCGCTGCGGGCACCTCGATGGCAAGTCGGTGATCCCGGCCGCCGAGATGGCGGGCAAGATCC
GCGCTGCCTGCGACGCGCGCCGCGATGCGAACACGCTGATCATCGCCCGTACCGACGCCGTGGCGGTGGAGGGCATGGAA
GCGGCGCTGGAACGCGCCGAGCGCTACGCCGAGGCCGGCGCCGATCTGCTGTTCGTGGAGGCGCTGCGCAGCCGCGAAGA
CATGTCGGCGGCCATCGCCAGGCTCGGGACACGCGCGCCGCTGCTGGCCAATATGGTCGAAGGCGGCAAGACGCCGGTGC
TGCCAGCGCCGGAACTGGAAGAGATCGGCTTTCGTGTCGTGATCTTCCCCGGCGGTACCGTACGGGCGCTGGCCTTTGCG
CTGCGCGACTACCTGCAAAGCCTGGGGACGCATCAGACCACCACACCGTACCTGGACCGGATGCTGTCGTTCCAGGCGCT
CAACGAGCTGATCGGCACACCTGAAATGCTGGCGCTGGGCAAGCGCTACGAATAG

Upstream 100 bases:

>100_bases
TTCCGCCGCACCTGGTGGACATGATCCAAGACGGCGGCCTGGTGCCGCACCTCGCCCGCAAGATCGCGGCGGGCCAACTT
TCCGTGAACGGAAATCCAGC

Downstream 100 bases:

>100_bases
GGCAGCATCGCTTGTTCAACTAGCGCGACGCGCGTACCGTGGCGGCAATGCCCGTACGCTTGTCAGCCTATTGCCCATGC
CTTTGGATGCCAACCCCGAT

Product: 2,3-dimethylmalate lyase

Products: NA

Alternate protein names: 2-methylisocitrate lyase [H]

Number of amino acids: Translated: 284; Mature: 283

Protein sequence:

>284_residues
MTLKQRLQQPGIVTAPGVYDAFSALLVEQAGFQAAYLSGASLAYTRFGRPDIGLLSLDDVASVTRNIRERVALPLIVDAD
TGFGNALNVVQTVRVLERAGASAIQLEDQAMPKRCGHLDGKSVIPAAEMAGKIRAACDARRDANTLIIARTDAVAVEGME
AALERAERYAEAGADLLFVEALRSREDMSAAIARLGTRAPLLANMVEGGKTPVLPAPELEEIGFRVVIFPGGTVRALAFA
LRDYLQSLGTHQTTTPYLDRMLSFQALNELIGTPEMLALGKRYE

Sequences:

>Translated_284_residues
MTLKQRLQQPGIVTAPGVYDAFSALLVEQAGFQAAYLSGASLAYTRFGRPDIGLLSLDDVASVTRNIRERVALPLIVDAD
TGFGNALNVVQTVRVLERAGASAIQLEDQAMPKRCGHLDGKSVIPAAEMAGKIRAACDARRDANTLIIARTDAVAVEGME
AALERAERYAEAGADLLFVEALRSREDMSAAIARLGTRAPLLANMVEGGKTPVLPAPELEEIGFRVVIFPGGTVRALAFA
LRDYLQSLGTHQTTTPYLDRMLSFQALNELIGTPEMLALGKRYE
>Mature_283_residues
TLKQRLQQPGIVTAPGVYDAFSALLVEQAGFQAAYLSGASLAYTRFGRPDIGLLSLDDVASVTRNIRERVALPLIVDADT
GFGNALNVVQTVRVLERAGASAIQLEDQAMPKRCGHLDGKSVIPAAEMAGKIRAACDARRDANTLIIARTDAVAVEGMEA
ALERAERYAEAGADLLFVEALRSREDMSAAIARLGTRAPLLANMVEGGKTPVLPAPELEEIGFRVVIFPGGTVRALAFAL
RDYLQSLGTHQTTTPYLDRMLSFQALNELIGTPEMLALGKRYE

Specific function: Catalyzes the formation of pyruvate and succinate from 2-methylisocitrate [H]

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily. Methylisocitrate lyase family [H]

Homologues:

Organism=Escherichia coli, GI1786525, Length=257, Percent_Identity=41.6342412451362, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI1790445, Length=181, Percent_Identity=31.4917127071823, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000918
- InterPro:   IPR018523
- InterPro:   IPR012695
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00463 ICL [H]

EC number: =4.1.3.30 [H]

Molecular weight: Translated: 30499; Mature: 30367

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS00161 ISOCITRATE_LYASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLKQRLQQPGIVTAPGVYDAFSALLVEQAGFQAAYLSGASLAYTRFGRPDIGLLSLDDV
CCCHHHHCCCCCEECCCHHHHHHHHHHHHCCCCEEHHCCCHHEEHCCCCCCCCEEEHHHH
ASVTRNIRERVALPLIVDADTGFGNALNVVQTVRVLERAGASAIQLEDQAMPKRCGHLDG
HHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHCCCCC
KSVIPAAEMAGKIRAACDARRDANTLIIARTDAVAVEGMEAALERAERYAEAGADLLFVE
CCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCHHHHHH
ALRSREDMSAAIARLGTRAPLLANMVEGGKTPVLPAPELEEIGFRVVIFPGGTVRALAFA
HHHCHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCHHHCCEEEEEECCCHHHHHHHH
LRDYLQSLGTHQTTTPYLDRMLSFQALNELIGTPEMLALGKRYE
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHCCCCCC
>Mature Secondary Structure 
TLKQRLQQPGIVTAPGVYDAFSALLVEQAGFQAAYLSGASLAYTRFGRPDIGLLSLDDV
CCHHHHCCCCCEECCCHHHHHHHHHHHHCCCCEEHHCCCHHEEHCCCCCCCCEEEHHHH
ASVTRNIRERVALPLIVDADTGFGNALNVVQTVRVLERAGASAIQLEDQAMPKRCGHLDG
HHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHCCCCC
KSVIPAAEMAGKIRAACDARRDANTLIIARTDAVAVEGMEAALERAERYAEAGADLLFVE
CCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCHHHHHH
ALRSREDMSAAIARLGTRAPLLANMVEGGKTPVLPAPELEEIGFRVVIFPGGTVRALAFA
HHHCHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCHHHCCEEEEEECCCHHHHHHHH
LRDYLQSLGTHQTTTPYLDRMLSFQALNELIGTPEMLALGKRYE
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10382966 [H]