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The map label for this gene is oprM [H]

Identifier: 73538313

GI number: 73538313

Start: 1155482

End: 1157020

Strand: Reverse

Name: oprM [H]

Synonym: Reut_B4486

Alternate gene names: 73538313

Gene position: 1157020-1155482 (Counterclockwise)

Preceding gene: 73538314

Following gene: 73538312

Centisome position: 42.44

GC content: 65.24

Gene sequence:

>1539_bases
ATGAACGCCGCCGATTTCTCCATGCGGGCCTCGATCCTGGCGATACTGGCCTGTCTTGCACTGGCGGGGTGTGCCGTGGG
GCCCGATTTCGAGCGCCCGACGCCTTCTGCGCCAGATGTCTTCCATCGCATCCAGTCCGCCCAGGCAAGCAGCAAGGCTG
TTGACGCGGAGTTCAACGTGGAGTGGTGGACGCTGCTGAACGATCCCCTGCTGAACAATCTCGAGAGCCGGCTGGTCGAC
GCGAATCTCGACGTGGCCGCGGCATCGGCGCGTTTGCGGCAAAGCCGCTCGACCCAACAGGTTGTCGGCGCGGCCCAGCT
TCCGCGGCTTGACGGCGCCGCATCCTATTATCGCGAGCGCGGGAGTCCGAACGGCATTGTCTCGCTGTTGGGGGTCAGCC
CAACGGGGAGCCCGCCGCAACCGGCGTCGGGTTCGTCGCCGCTCGGGGTTGCGCCGGTGCCAGGATCGAGCGGTTCCCCG
CCCTTTGACCTGTACCAGTTTGGCTTCGATGCGTCCTGGGAAATCGACATCTGGGGCCGGGTGCGGCGCAGTGTCGAAGC
TGCCGGCGCATTGACGGAAGCTTCGTTCGAGGACAGGAACGCCATCCTCTTGTCTGCCCGCGCCGAACTTGCACGGGACT
ATGTGGAACTTCGCGACCTCCAGTCGCTGCTGGAAATCACGAAGCAGAACCTTGAGATTGCTCGTGACACCGCCAGGCTG
ACGCAGGTCCGTGCACGCGAGGGCGTGACAACGGATCTCGATGTAGCCAATGCCGCAGCCCAGGTGGCGTCGATCGAAAG
CCTGATGCCGACGCTGGAAGCGCGGCTCGAAGCTGCCATCAACGCCATCGGCTTGCTGCTGGCCGAGGAGCCTGGCGCGT
TGCGGCAGGAACTTGCGGAACGGCGTGAAGTGCCAGAGCTGCCGGAGCGCGTGCCGGTCGGCTTCCCGTCGGAGATCGTG
CAGCGCAGGCCTGACATCAGGCGAGCCGAGGCGCAGTTGCACGCGGCCACGGCATCGATCGGGATGGCCAAGGCCGACTT
CTATCCGCGGATTTCGCTGAACGGAAGCGCCGGATTCCAGAGTCTGCAGCTTTCCAGCCTTGCCGACTGGGCCTCGCGCC
AGTTTGTGGTCGGGCCGTCGATCACGTTTCCGATCTTCGAGGGCGGTCGACTCAAGGGAACGCTGCATCTGCGCGAGGCC
CAGCAACAGGAGGCCGCTATCGCGTACAAGCGCACCGTGCTCGACGCATGGCGCGAGGTTGACGACGCGCTGGTCGCCTA
CGATGCCGAGCAGCGGCGGCGCGACCGGCTCGTGACGGTCGTGAGCGAGAACCAGCGTGCTCTCGCGATTGCGCAGCGGC
GGTACAAGGCCGGAGCAGTCGATTATCTCGACGTGCTGAACGTGCAGAGACAGCTCCTGCGGTCGCAAAGCAATCTCGAG
CAAAGCCGCGCAACGGCGGCCACGAACCTCATAACCCTGTGCAAGGTCCTCGGCGGAGGCTGGGAATCTGCGTACGCCAG
GACCCCGACTGCGAACTGA

Upstream 100 bases:

>100_bases
GGCAGAAGGCCGCATCCGGATTGAGCGTGGGTCTTTCGGTAGAGGTGGACGTCGCCATTGCCCGTCGTGAAGATGTGCTG
CCCCAGGGAGGTAACAGAGG

Downstream 100 bases:

>100_bases
GCGCGCGAGTGGCCAGAAACACGCAGAATCTGGCCACAATCCTGATCAAGTCGTTGGATCGGCACGCGATGTCCAAACCT
ATACTTGCCCGGGCAGTGCA

Product: RND efflux system outer membrane lipoprotein NodT

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 512; Mature: 512

Protein sequence:

>512_residues
MNAADFSMRASILAILACLALAGCAVGPDFERPTPSAPDVFHRIQSAQASSKAVDAEFNVEWWTLLNDPLLNNLESRLVD
ANLDVAAASARLRQSRSTQQVVGAAQLPRLDGAASYYRERGSPNGIVSLLGVSPTGSPPQPASGSSPLGVAPVPGSSGSP
PFDLYQFGFDASWEIDIWGRVRRSVEAAGALTEASFEDRNAILLSARAELARDYVELRDLQSLLEITKQNLEIARDTARL
TQVRAREGVTTDLDVANAAAQVASIESLMPTLEARLEAAINAIGLLLAEEPGALRQELAERREVPELPERVPVGFPSEIV
QRRPDIRRAEAQLHAATASIGMAKADFYPRISLNGSAGFQSLQLSSLADWASRQFVVGPSITFPIFEGGRLKGTLHLREA
QQQEAAIAYKRTVLDAWREVDDALVAYDAEQRRRDRLVTVVSENQRALAIAQRRYKAGAVDYLDVLNVQRQLLRSQSNLE
QSRATAATNLITLCKVLGGGWESAYARTPTAN

Sequences:

>Translated_512_residues
MNAADFSMRASILAILACLALAGCAVGPDFERPTPSAPDVFHRIQSAQASSKAVDAEFNVEWWTLLNDPLLNNLESRLVD
ANLDVAAASARLRQSRSTQQVVGAAQLPRLDGAASYYRERGSPNGIVSLLGVSPTGSPPQPASGSSPLGVAPVPGSSGSP
PFDLYQFGFDASWEIDIWGRVRRSVEAAGALTEASFEDRNAILLSARAELARDYVELRDLQSLLEITKQNLEIARDTARL
TQVRAREGVTTDLDVANAAAQVASIESLMPTLEARLEAAINAIGLLLAEEPGALRQELAERREVPELPERVPVGFPSEIV
QRRPDIRRAEAQLHAATASIGMAKADFYPRISLNGSAGFQSLQLSSLADWASRQFVVGPSITFPIFEGGRLKGTLHLREA
QQQEAAIAYKRTVLDAWREVDDALVAYDAEQRRRDRLVTVVSENQRALAIAQRRYKAGAVDYLDVLNVQRQLLRSQSNLE
QSRATAATNLITLCKVLGGGWESAYARTPTAN
>Mature_512_residues
MNAADFSMRASILAILACLALAGCAVGPDFERPTPSAPDVFHRIQSAQASSKAVDAEFNVEWWTLLNDPLLNNLESRLVD
ANLDVAAASARLRQSRSTQQVVGAAQLPRLDGAASYYRERGSPNGIVSLLGVSPTGSPPQPASGSSPLGVAPVPGSSGSP
PFDLYQFGFDASWEIDIWGRVRRSVEAAGALTEASFEDRNAILLSARAELARDYVELRDLQSLLEITKQNLEIARDTARL
TQVRAREGVTTDLDVANAAAQVASIESLMPTLEARLEAAINAIGLLLAEEPGALRQELAERREVPELPERVPVGFPSEIV
QRRPDIRRAEAQLHAATASIGMAKADFYPRISLNGSAGFQSLQLSSLADWASRQFVVGPSITFPIFEGGRLKGTLHLREA
QQQEAAIAYKRTVLDAWREVDDALVAYDAEQRRRDRLVTVVSENQRALAIAQRRYKAGAVDYLDVLNVQRQLLRSQSNLE
QSRATAATNLITLCKVLGGGWESAYARTPTAN

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=499, Percent_Identity=28.6573146292585, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI2367348, Length=449, Percent_Identity=24.4988864142539, Blast_Score=115, Evalue=8e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 55519; Mature: 55519

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAADFSMRASILAILACLALAGCAVGPDFERPTPSAPDVFHRIQSAQASSKAVDAEFNV
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCC
EWWTLLNDPLLNNLESRLVDANLDVAAASARLRQSRSTQQVVGAAQLPRLDGAASYYRER
EEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHC
GSPNGIVSLLGVSPTGSPPQPASGSSPLGVAPVPGSSGSPPFDLYQFGFDASWEIDIWGR
CCCCCEEEEEECCCCCCCCCCCCCCCCCCEECCCCCCCCCCCHHHHCCCCCCEEEEHHHH
VRRSVEAAGALTEASFEDRNAILLSARAELARDYVELRDLQSLLEITKQNLEIARDTARL
HHHHHHHHCCHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TQVRAREGVTTDLDVANAAAQVASIESLMPTLEARLEAAINAIGLLLAEEPGALRQELAE
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
RREVPELPERVPVGFPSEIVQRRPDIRRAEAQLHAATASIGMAKADFYPRISLNGSAGFQ
HCCCCCCHHHCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHCCHHCCCCCEEEECCCCCCH
SLQLSSLADWASRQFVVGPSITFPIFEGGRLKGTLHLREAQQQEAAIAYKRTVLDAWREV
HHHHHHHHHHHCCCEEECCCEEEEEECCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHH
DDALVAYDAEQRRRDRLVTVVSENQRALAIAQRRYKAGAVDYLDVLNVQRQLLRSQSNLE
HHHHHHCCHHHHHHHHEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
QSRATAATNLITLCKVLGGGWESAYARTPTAN
HHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCC
>Mature Secondary Structure
MNAADFSMRASILAILACLALAGCAVGPDFERPTPSAPDVFHRIQSAQASSKAVDAEFNV
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCC
EWWTLLNDPLLNNLESRLVDANLDVAAASARLRQSRSTQQVVGAAQLPRLDGAASYYRER
EEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHC
GSPNGIVSLLGVSPTGSPPQPASGSSPLGVAPVPGSSGSPPFDLYQFGFDASWEIDIWGR
CCCCCEEEEEECCCCCCCCCCCCCCCCCCEECCCCCCCCCCCHHHHCCCCCCEEEEHHHH
VRRSVEAAGALTEASFEDRNAILLSARAELARDYVELRDLQSLLEITKQNLEIARDTARL
HHHHHHHHCCHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TQVRAREGVTTDLDVANAAAQVASIESLMPTLEARLEAAINAIGLLLAEEPGALRQELAE
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
RREVPELPERVPVGFPSEIVQRRPDIRRAEAQLHAATASIGMAKADFYPRISLNGSAGFQ
HCCCCCCHHHCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHCCHHCCCCCEEEECCCCCCH
SLQLSSLADWASRQFVVGPSITFPIFEGGRLKGTLHLREAQQQEAAIAYKRTVLDAWREV
HHHHHHHHHHHCCCEEECCCEEEEEECCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHH
DDALVAYDAEQRRRDRLVTVVSENQRALAIAQRRYKAGAVDYLDVLNVQRQLLRSQSNLE
HHHHHHCCHHHHHHHHEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
QSRATAATNLITLCKVLGGGWESAYARTPTAN
HHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]