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The map label for this gene is emrA [H]

Identifier: 73538314

GI number: 73538314

Start: 1157017

End: 1158093

Strand: Reverse

Name: emrA [H]

Synonym: Reut_B4487

Alternate gene names: 73538314

Gene position: 1158093-1157017 (Counterclockwise)

Preceding gene: 73538315

Following gene: 73538313

Centisome position: 42.48

GC content: 63.97

Gene sequence:

>1077_bases
ATGTCATCCGCTACCCGCCCGCCAATCAGGAATATCCGCCTTGCAGCGATCTTCATCGCGGTCGGCATCGTTGCGTGGGC
CTGCGCAACCCTCGTTCCAGGCTCAAGTGCGCAGTCCACCAATGATGCCTATGTAACGGCAGACTTCACGCTCGTAGCCC
CACGTATCGCTGGCCAGATTTCCGAGGTGCTTGTGGATGACAACCAATGGGTCAAGGCCGGGCAGGTCCTGGTCCGTATC
GATGACCGCGACTTCAGGGCGGCGCTGCAAAGTGCGCAGGCAGACGTTCTGGCGGCCAAAGCCATGGTGGCGAACTATGA
CGCGGAGATCGCCCGGCATCCGTCCCTGGTCGAGCAGGCACGGGCGAAGCTCCGGTCCGACGAAGCATCGGTCGAGTTTG
CCCGCGCCAACGCGTCGCGCTACCGGAATCTTTCGGAAGCCGGAGCCGGCACCGAGCAGGAGCAGCAACGTGCGTCCAGT
ACGCTTGCCGAGCAGGTAGCGCAGCAGTCCCGCGACCGCGCCGCGCTGAGCACCACCGAACAGAACCTCATGGTCCTGAA
CACGCAGCGCGACAAGGCCGCGGGAGCCCTGGCGCGTGCCGAAGCCGCACTGGAACAGGCAAGGCTCAATCTGTCGTATG
CGGAAATCCACGCGCCGGTGGATGGCAAGATCGGCAGGCGTTCGGCGCGTGTCGGTGCATACGTGACGGCAGGCGCTTCG
GTCCTTGCCATCGTGCCGGTGTCGGACGCGTACGTGGTGGCCAATTTCCAGGAGAACCAGATTGCCGGCATGCGGCCGGG
ACAGAGCGCGCGCATCAAGGTTGACAGCCTGCCTGGCGTCGAGATCCAGGGCCATATCCACAGCCTGGCGCCCGCTACGG
GAGTGAGCTTCGCGCCCATTGCCCCGGATAACGCCACGGGCAACTTCACCAAGATCGTCCAACGCGTGCCGGTCAAGATC
ACCATCGATCGCGGGCAGAAGGCCGCATCCGGATTGAGCGTGGGTCTTTCGGTAGAGGTGGACGTCGCCATTGCCCGTCG
TGAAGATGTGCTGCCCCAGGGAGGTAACAGAGGATGA

Upstream 100 bases:

>100_bases
CGTTGCCATTGCCTTGCTGTTTCTCGTTCCGATATTGCCCGTGCGCATCTATCCGCCCTGGAGCACTACGCCACCCTCTT
CACACTAAGGGAAGATCATC

Downstream 100 bases:

>100_bases
ACGCCGCCGATTTCTCCATGCGGGCCTCGATCCTGGCGATACTGGCCTGTCTTGCACTGGCGGGGTGTGCCGTGGGGCCC
GATTTCGAGCGCCCGACGCC

Product: secretion protein HlyD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 358; Mature: 357

Protein sequence:

>358_residues
MSSATRPPIRNIRLAAIFIAVGIVAWACATLVPGSSAQSTNDAYVTADFTLVAPRIAGQISEVLVDDNQWVKAGQVLVRI
DDRDFRAALQSAQADVLAAKAMVANYDAEIARHPSLVEQARAKLRSDEASVEFARANASRYRNLSEAGAGTEQEQQRASS
TLAEQVAQQSRDRAALSTTEQNLMVLNTQRDKAAGALARAEAALEQARLNLSYAEIHAPVDGKIGRRSARVGAYVTAGAS
VLAIVPVSDAYVVANFQENQIAGMRPGQSARIKVDSLPGVEIQGHIHSLAPATGVSFAPIAPDNATGNFTKIVQRVPVKI
TIDRGQKAASGLSVGLSVEVDVAIARREDVLPQGGNRG

Sequences:

>Translated_358_residues
MSSATRPPIRNIRLAAIFIAVGIVAWACATLVPGSSAQSTNDAYVTADFTLVAPRIAGQISEVLVDDNQWVKAGQVLVRI
DDRDFRAALQSAQADVLAAKAMVANYDAEIARHPSLVEQARAKLRSDEASVEFARANASRYRNLSEAGAGTEQEQQRASS
TLAEQVAQQSRDRAALSTTEQNLMVLNTQRDKAAGALARAEAALEQARLNLSYAEIHAPVDGKIGRRSARVGAYVTAGAS
VLAIVPVSDAYVVANFQENQIAGMRPGQSARIKVDSLPGVEIQGHIHSLAPATGVSFAPIAPDNATGNFTKIVQRVPVKI
TIDRGQKAASGLSVGLSVEVDVAIARREDVLPQGGNRG
>Mature_357_residues
SSATRPPIRNIRLAAIFIAVGIVAWACATLVPGSSAQSTNDAYVTADFTLVAPRIAGQISEVLVDDNQWVKAGQVLVRID
DRDFRAALQSAQADVLAAKAMVANYDAEIARHPSLVEQARAKLRSDEASVEFARANASRYRNLSEAGAGTEQEQQRASST
LAEQVAQQSRDRAALSTTEQNLMVLNTQRDKAAGALARAEAALEQARLNLSYAEIHAPVDGKIGRRSARVGAYVTAGASV
LAIVPVSDAYVVANFQENQIAGMRPGQSARIKVDSLPGVEIQGHIHSLAPATGVSFAPIAPDNATGNFTKIVQRVPVKIT
IDRGQKAASGLSVGLSVEVDVAIARREDVLPQGGNRG

Specific function: The emr locus confers resistance to substances of high hydrophobicity. EmrA probably participate in a transport system to extrude toxins and drugs from the cell [H]

COG id: COG1566

COG function: function code V; Multidrug resistance efflux pump

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789041, Length=336, Percent_Identity=29.1666666666667, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1788711, Length=319, Percent_Identity=29.7805642633229, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1790519, Length=311, Percent_Identity=29.2604501607717, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1789637, Length=317, Percent_Identity=28.391167192429, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI87081951, Length=333, Percent_Identity=26.1261261261261, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1790024, Length=244, Percent_Identity=23.7704918032787, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1789900, Length=344, Percent_Identity=26.1627906976744, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1790012, Length=252, Percent_Identity=25.3968253968254, Blast_Score=71, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005694
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 37836; Mature: 37705

Theoretical pI: Translated: 8.98; Mature: 8.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSATRPPIRNIRLAAIFIAVGIVAWACATLVPGSSAQSTNDAYVTADFTLVAPRIAGQI
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEECHHHHHHH
SEVLVDDNQWVKAGQVLVRIDDRDFRAALQSAQADVLAAKAMVANYDAEIARHPSLVEQA
HHHHCCCCCEEECCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCHHHHHHH
RAKLRSDEASVEFARANASRYRNLSEAGAGTEQEQQRASSTLAEQVAQQSRDRAALSTTE
HHHHCCCCCCEEEECCCHHHHCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
QNLMVLNTQRDKAAGALARAEAALEQARLNLSYAEIHAPVDGKIGRRSARVGAYVTAGAS
CCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCHHEEECCCCCCCCCCHHHCCEEEECCCE
VLAIVPVSDAYVVANFQENQIAGMRPGQSARIKVDSLPGVEIQGHIHSLAPATGVSFAPI
EEEEEECCCEEEEEECCCCCCCCCCCCCCCEEEEECCCCEEEECCHHHCCCCCCCEECCC
APDNATGNFTKIVQRVPVKITIDRGQKAASGLSVGLSVEVDVAIARREDVLPQGGNRG
CCCCCCCHHHHHHHHCCEEEEECCCCCCCCCCEEEEEEEEEEEEECCCCCCCCCCCCC
>Mature Secondary Structure 
SSATRPPIRNIRLAAIFIAVGIVAWACATLVPGSSAQSTNDAYVTADFTLVAPRIAGQI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEECHHHHHHH
SEVLVDDNQWVKAGQVLVRIDDRDFRAALQSAQADVLAAKAMVANYDAEIARHPSLVEQA
HHHHCCCCCEEECCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCHHHHHHH
RAKLRSDEASVEFARANASRYRNLSEAGAGTEQEQQRASSTLAEQVAQQSRDRAALSTTE
HHHHCCCCCCEEEECCCHHHHCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
QNLMVLNTQRDKAAGALARAEAALEQARLNLSYAEIHAPVDGKIGRRSARVGAYVTAGAS
CCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCHHEEECCCCCCCCCCHHHCCEEEECCCE
VLAIVPVSDAYVVANFQENQIAGMRPGQSARIKVDSLPGVEIQGHIHSLAPATGVSFAPI
EEEEEECCCEEEEEECCCCCCCCCCCCCCCEEEEECCCCEEEECCHHHCCCCCCCEECCC
APDNATGNFTKIVQRVPVKITIDRGQKAASGLSVGLSVEVDVAIARREDVLPQGGNRG
CCCCCCCHHHHHHHHCCEEEEECCCCCCCCCCEEEEEEEEEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]